JP2007016013A - Oral composition for menopausal skin improvement - Google Patents

Abstract

[PROBLEMS] To provide an oral composition having an effect of improving skin wrinkles, sagging, hardness, and elasticity by improving skin thickness which decreases in menopause.
An oral composition for improving the skin of menopause is used, which contains diosgenin contained in a rhizome powder or an extract obtained from the rhizome of a genus Phyllidae plant. In particular, the genus Dioscorea virosa is used.
[Effect] It is highly safe and can reduce skin changes that occur after menopause and after menopause.
[Selection figure] None

Description

  The present invention relates to a menopausal oral improvement composition for skin improvement comprising diosgenin as an active ingredient.

  Organs of all living organisms including humans are born and grow, then gradually decline with age, eventually stop functioning, and die when a certain part of the function is stopped. A state in which the function gradually declines is called aging. The skin is directly affected by the surrounding environment, and since it has an important function to maintain the environment inside the living body, it is not likely that everything will stop functioning, but aging such as wrinkles, spots, dullness, and tarmi This is an organ in which signs are easily manifested, and is particularly prominent in exposed areas exposed to sunlight.

  The female body matures from early childhood to adolescence, and the spring process that is the completion of the maturation process repeats a periodic change in sex hormone balance called the sexual cycle, enabling pregnancy It becomes a state. Thereafter, although there are individual differences, the sex hormone secretion becomes irregular from the late 30s to the 50s with the decline of ovarian function, and when the ovarian function is lost, the amount of sex hormone in the body drastically decreases. The period until this sex hormone secretion is irregular and the ovarian function is lost is called menopause, and many people suffer from indefinite complaints such as hot flashes along with dramatic changes in the internal environment. In addition, since the skin is also affected by sex hormones derived from the ovaries, the skin layer becomes thinner after menopause and after menopause, resulting in an unfavorable cosmetic condition such as increased wrinkle formation.

  In response to various changes in menopause, administration of sex steroid hormones has been performed mainly in the United States, and this method is effective in reducing excessive climacteric symptoms. On the other hand, sex steroid hormones work by binding to receptors in the cell nucleus (Non-patent Document 1), and it is also known that administration of sex steroid hormone agents increases the risk of developing diseases such as breast cancer. (Non-patent document 2). Therefore, the administration of sex steroid hormone agents is being avoided in Japan as well as in Japan in terms of safety. In addition, steroid hormone precursors are converted into sex steroid hormones (Non-patent Document 3), and there is a report (Patent Document 1) that application of the precursor has improved skin aging signs and dryness, and oral intake Reported reduction or prevention of skin paper-like appearance (Patent Document 2) and improvement of skin moisture content (Patent Document 3). However, ingestion of sex steroid hormone precursors is also converted from sex steroid hormones, which is problematic in terms of safety.

  There are reports that the symptoms of menopause are lighter in women in Japan than in Western women, and that the factor is due to the intake of soy isoflavone having a female hormone-like action (Non-patent Document 4). However, having a female hormone-like action increases the risk of breast cancer, and it has been reported that soybean isoflavone has increased the risk of breast cancer (Non-patent Document 5). However, these effects are insufficient to improve menopausal skin symptoms, and there are doubts about their safety. Therefore, there are no satisfactory substances with effective drugs that are highly safe for menopausal symptoms, especially skin symptoms.

On the other hand, as a substance for improving pigmentation due to overproduction of melanin pigments with aging by oral administration, Dioscorea compositor which is a genus Dioscorea and rhizome ground extract or extract of Virosa have been proposed (Patent Literature) 4). However, its effect prevents melanin pigment overproduction in melanocytes. It has also been reported that melanin synthesis decreases and skin color turns white in menopause and non-pigmented skin after menopause (Non-patent Document 6). Therefore, it is unclear whether diosgenin, the active main component of Dioscorea virosa, has an effect on menopausal skin.

JP 2003-26697 A JP 2003-519648 A JP 2003-520238 A JP 2004-066763 A Beato M. et al. “Gene regulation by steroid homone.” Cell. 1989; 56 (3): p. 335-44 “Estrogen plus Progestin Effects on Breast Cancer and Mammograms” [online] Woman's Health Initiative [Search June 1, 2005] Internet <URL: http://www.whi.org/findings/epluspbc > Arlt W.H. “Dehydroepiandrosterone and ageing.” Best Pract Res Clin Endocrinol Metab. 2004; 18 (3): p. 363-80 Wu AH, Pike MC. “Dietary soy protein and harmonic status in females.” Am J Clin Nutr. 1995; 62 (1): p. 151-3 McMichael-Phillips DF, Harding C, Morton M, et al. “Effects of soy-protein supplementation on epithelial proliferation in histologic normal human breast.” Am J Clin Nutr. 1998; 68 (Suppl): p. 1431-5 Roh KY, Kim D, Ha S-J, Ro Y J, et al. “Pigmentation in Koreas: study of the differentials from caucasians in age, gender and seasonal variations.” Br J Dermatol. 2001; 144 (1): p. 94-9

  Under such circumstances, an object of the present invention is to provide a highly safe oral menopausal skin-improving agent capable of reducing skin changes occurring after menopause and after menopause.

This invention exists in the oral composition used in order to improve the menopausal skin symptom which contains a diosgenin as an active ingredient.
Further, the present invention is characterized in that the origin of the diosgenin is a rhizome powder or an extract obtained from a rhizome part, and further, the yam plant is a dioscorea virosa (Dioscorea). Villosa).

  The present invention provides an oral composition that is highly safe and can reduce skin changes that occur after menopause and after menopause.

  The diosgenin used in the present invention can be applied to a chemically synthesized product or a naturally derived product extracted from a plant. In particular, a rhizome pulverized product or an extract of a genus Dioscorea plant, and a genus Dioscorea plant is Dioscorea Villosa. A rhizome part pulverized product or extract is preferable because it is easily available and inexpensive.

  The plant of the genus Yamaceae used in the present invention is a vine perennial plant that uses the rhizome as an edible material. In addition, it is known that hydrochalcone derivatives and the like exist as whitening active substances in the rhizomes of Dioscorea Composita produced in Latin America, and are applied as cosmetics (Japanese Patent Laid-Open No. 09-212439). Issue gazette). Furthermore, Dioscorea virosa is also a common plant of the genus Yamaceae, and has an inhibitory effect on tyrosinase activity, and thus has been applied as an agent for improving pigmentation associated with oral aging (Japanese Patent Laid-Open No. 2004-67663).

  The powder is obtained by drying and crushing the rhizome, and as a solvent for obtaining an extract from the powder, water, alcohols such as ethanol, methanol, isopropyl alcohol, ethylene glycol, propylene are generally used. Polyols such as glycol and 1,3-butylene glycol, ketones such as acetone, esters such as ethyl acetate, ethers such as diethyl ether, and one or a mixture of two or more aromatic compounds such as benzene Is used.

  The oral composition of the present invention can be used without particular limitation as long as it is orally ingested, such as specific products / uses, properties, etc., and can be used in liquid, gel or solid foods such as alcoholic beverages, carbonic acid Beverages, beverages with juice, coffee beverages, tea beverages, soft drinks such as fruit syrup, lactic acid bacteria beverages and foods such as soups, dressings, jellies, puddings, yogurts, milk beverages and foods, sprinkles, powdered soups and cake mixes Capsules can be added to powdered products, bread, cookies, strawberries, frozen desserts such as gums and ice cream, and coated with pellets, granules, tablets, or gelatin as appropriate with excipients such as dextrin, fragrances, and pigments. It can be processed into health foods and nutritional supplements. 0.001-30 mass% is preferable, and, as for the compounding quantity of diosgenin in these oral compositions, Most preferably, it is 0.01-16 mass%.

  The oral composition for improving menopause skin of the present invention improves the thinning of the skin and mucous membranes due to a decrease in ovarian function and the accompanying decrease in sex hormone secretion. It has an effect of improving sagging, hardness and beam.

  Hereinafter, the present invention will be described in detail based on examples and comparative examples.

Examples 1-4 and Comparative Examples 1-2
The effects on the skin of ovariectomized (OVX) hairless mice, which are commonly used as menopausal model animals, were examined.

1. Substances used in Examples and Comparative Examples The rhizome part of Dioscorea virosa was dried and cut, and powder extracted with 80 vol% ethanol was used as an evaluation sample. Furthermore, diosgenin (manufactured by Sigma), which is an active body of rhizome pulverized product or extract of Dioscorea vilosa, was purchased and used.

2. Animals used in the examples In this study, females of SKH: HR-1 mice, which are hairless mice, were subjected to ovariectomy (OVX) or sham (Shm) surgery at Charles River, USA, and purchased at 6 weeks of age. Preliminary breeding was carried out in a breeding facility (environmental temperature 22 ± 1 ° C., relative humidity 60 ± 10%, light / dark cycle 12L: 12D), and 10 weeks of age was used.

3. Sample Administration and Experimental Method As test samples, Dioscorea vilosa and diosgenin were dispersed in a base olive oil (manufactured by Wako Pure Chemical Industries, Ltd.) and used. As an example, each individual OVX mouse was administered with the doses of diosgenin shown in Table 1 or Dioscorea virosa directly into the stomach with a sonde. As a comparative example, only the base was administered directly into the stomach for each OVX mouse and Shm mouse. It was administered once a day for 5 consecutive days and administered for 17 weeks. Three days after the last treatment, the back skin of each individual was collected, paraffin sections were prepared according to a conventional method, HE staining was performed, and the epidermis thickness was measured under a microscope.

Table 1 shows the effect on the skin thickness of OVX mice.
[Table 1]
Group Skin thickness (μm)
Example 1 (OVX mouse: Dioscorea virosa) 16.1 ± 0.77
Example 2 (OVX mouse: diosgenin 20 mg / kg) 15.7 ± 0.68
Example 3 (OVX mouse: diosgenin 40 mg / kg) 16.1 ± 1.14
Example 4 (OVX mouse: diosgenin 80 mg / kg) 16.9 ± 0.64
Comparative Example 1 (Shm mouse: base) 18.3 ± 0.71
Comparative Example 2 (OVX mouse: base) 15.6 ± 0.52
Values are average values ± standard error (n = 8−10)

  Compared with Comparative Example 1, Comparative Example 2 had a thinner skin thickness. This is generally said in menopause, and this model is a model for menopause. Compared to Comparative Example 2, the skin thickness was increased in the Examples. Thus, it has been found that the embodiments of the present invention improve the skin thickness that becomes thinner in the menopause.

  In Examples 1 to 4 and Comparative Examples 1 and 2 of this test, weight loss due to failure of oral administration or the like was not observed, a normal growth curve was obtained, and the result of this test was not simply due to poor physical condition. It became clear.

Example 5, comparative example 3 (human continuous test)
Prepare a juice-containing beverage consisting of the following composition by a regular method, and have 20 persons in their menopause and subsequent 45-60s drink 3 times daily (at meal time) 100 mL / time for 1 month, then skin A questionnaire survey was conducted on wrinkles, sagging, hardness and beam.

[Table 2] (Example 5)
Ingredient name Mass%
Dioscorea virosa powder used in Example 1 5.0
Orange juice 5.0
Fructose glucose liquid sugar 7.0
Vitamin C 1.0
Water 82.0

[Table 3] (Comparative Example 3)
Ingredient name Mass%
Orange juice 5.0
Fructose glucose liquid sugar 7.0
Vitamin C 1.0
Water 87.0

The results are shown below.
[Table 4]
Item Example 5 evaluation Comparative example 3 evaluation
Improvement Invariant Improvement Invariant skin wrinkles 15 5 1 19
Skin sagging 17 3 2 18
Hardness of skin 16 4 3 17
Skin tension 18 2 1 19

  From the above results, it was found that wrinkles, sagging, firmness and elasticity of the skin were improved by ingestion of a beverage containing juice containing Dioscorea virosa.

  In addition, when the binding of diosgenin to the receptor that binds when sex steroid hormones act was examined, no binding of diosgenin was observed in any of the estrogen receptor α, estrogen receptor β, and progesterone receptor. Has no sex hormone-like action, and it has been shown that administration of sex steroid hormones does not increase the risk of developing diseases such as breast cancer.

  Next, formulation examples in the case where the present invention is applied to powdered soups, rice cakes and tablets will be shown.

Powdered soup: According to the following prescription, each component was mixed to obtain a powdered soup. When drinking, 8 g of powder soup is added to 150 mL of hot water and used with sufficient stirring.
[Table 5]
Ingredient name Mass%
Dioscorea virosa powder used in Example 1 5.0
Scallop end 7.0
Salt 20.0
Skipjack extract 30.0
Powdered soy sauce 15.0
Pepper 0.9
Dietary fiber 22.1

飴: According to the following formulation, each component was kneaded uniformly into a water jar heated to 80 ° C., poured into a mold, and cooled to room temperature to obtain cocoons.
[Table 6]
Ingredient name Mass%
Dioscorea virosa powder used in Example 1 10.0
Minamata 30.0
Sucrose 20.0
Lactose 20.0
Dextrin 10.0
Vitamin C 5.0
Vitamin E 3.0
Calcium phosphate 1.0
Fragrance 1.0

Tablet: According to the following formulation, each component was mixed uniformly, and then punched to obtain a tablet.
[Table 7]
Ingredient name Mass%
Dioscorea virosa powder 30.0 used in Example 1
Vitamin C 10.0
Sucrose fatty acid ester 5.0
Crystalline cellulose 20.0
Lactose 35.0

  As described above, the present invention provides an oral skin improver that is excellent in safety, is excellent in the effect of improving skin thickness that decreases in menopause, and has an effect of improving skin wrinkles, sagging, hardness, and elasticity. it can.

Claims (3)

An oral composition for improving skin during menopause, comprising diosgenin. 2. The oral composition for improving skin of menopause according to claim 1, wherein the origin of diosgenin is a rhizome powder or an extract obtained from a rhizome part of the genus Phyllidae. The oral composition for improving the skin of menopause according to claim 2, wherein the genus Phyllidae is Dioscorea Villosa.