JP2006333844A - Compounding agent for fodder - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a compounding agent for fodder comprising safe ingredients derived from natural sources, more effectively preventing diarrhea of animals, improving healthy conditions of animals through regulation of intestinal environment and further promoting their growth, and a fodder compounded with the same. <P>SOLUTION: The compounding agent for fodder contains mannose and/or manno-oligosaccharide and sterilized microbe cells as effective ingredients. In the agent, mannose and/or manno-oligosaccharide are preferably decomposition products of mannan and the sterilized microbe cells are preferably crushed product of yeast cells or sterilized cells of lactobacillus. The fodder for animals is compounded with the compounding agent for fodder. <P>COPYRIGHT: (C)2007,JPO&INPIT

Description

  The present invention relates to a feed formulation for animal feed.

  Livestock such as chickens, pigs, sheep and cows are known to cause diarrhea when infected with harmful bacteria such as Escherichia coli or Salmonella or pathogenic viruses during childhood. If these livestock cause diarrhea, they will not be able to absorb enough nutrients from the intestinal tract and growth will stagnate, and their physical strength and resistance will decrease, making it more susceptible to other infections. is there. In addition, fish and shellfish such as aquaculture fish also have a problem that if they are infected with pathogenic microorganisms such as bacteria and viruses, their growth stagnate and the death rate increases.

  On the other hand, in the recent livestock industry, efforts have been made to increase the productivity of livestock and obtain higher economic effects. For example, it has been attempted to increase productivity by increasing the number of captive animals per area, but conversely, stress due to overcrowded breeding is said to reduce the physical strength and resistance of livestock and adversely affect productivity. Yes. Furthermore, once an infection occurs in an overcrowded barn, it spreads throughout the barn and further deteriorates productivity. On the other hand, antibiotics and antibacterial drugs have been mixed with feed for the purpose of preventing disease and promoting growth in livestock. The problem of the safety of livestock products has been pointed out, and there is a demand for safe materials to replace antibiotics and other drugs.

In order to meet such a demand, additives derived from natural products have been proposed. Specifically, feeds for improving meat quality and promoting growth of pigs containing koji leaves, malt, koji leaves, carrots, daikon, mountain medicines, yellow spirits, gold and silver flowers, koji kinko, or chickweed are known. (For example, refer to Patent Document 1). Moreover, the feed additive for livestock which consists of sugar flavor, licorice, and a stevia extract for the purpose of the palatability improvement and diarrhea reduction is known (for example, refer patent document 2). Furthermore, a feed additive for pigs containing nine kinds of herbs, yeast, lactic acid bacteria, and organic acids is known (see, for example, Patent Document 3). Or the feed additive for animals which consists of an herb, a yeast cell wall, a living fungus, and an organic acid is known (for example, refer to patent documents 4).
JP 2004-222547 A JP 2001-95502 A JP 2004-49174 A JP 2002-58434 A

  However, the conventional technique has not been fully effective.

  The present invention is composed of safe ingredients derived from natural products, and more effectively prevents animal diarrhea, improves the intestinal environment more effectively, improves animal health, and further promotes growth. It aims at providing the feed compounding agent for this and the feed which mix | blended it.

  As a result of diligent studies to solve such problems, the present inventors have prevented diarrhea by providing animals with a diet containing mannose and / or mannooligosaccharides, or both, and dead cells of microorganisms. In addition, the present inventors have found that when the health condition is synergistically improved, and when these feeds are given to livestock, the productivity of livestock is improved and the present invention has been achieved.

  That is, the first of the present invention is a feed formulation characterized by comprising mannose and / or mannooligosaccharide and dead microorganisms of microorganisms as active ingredients, preferably mannose and / or Alternatively, the manno-oligosaccharide is a mannan degradation product, and preferably, the dead cell of the microorganism is a yeast cell disrupted product or a lactic acid bacteria killed cell.

  The second aspect of the present invention is an animal feed characterized by blending the above-mentioned feed compounding agent.

  According to the present invention, a safe ingredient derived from a natural product can sufficiently exhibit effects such as preventing diarrhea in animals and promoting growth more effectively. It is effective when applied to young livestock and poultry such as chicks.

  The present invention will be described in detail below. First, mannose and mannooligosaccharide used in the present invention will be described. Mannose and mannooligosaccharide are obtained by hydrolyzing a naturally-occurring mannan-containing product with an acid or an enzyme, and may be a purified product or a crude product. Examples of naturally-occurring mannan-containing products include polysaccharides derived from microorganisms such as yeast cell walls, as well as plant-derived polysaccharides such as guar gum, locust bean gum, palm kernel, and copra meal. In order to obtain mannose and mannooligosaccharides from these mannan-containing materials by enzymatic treatment, a known method in which an enzyme such as mannanase, mannosidase, glucosidase, galactosidase, hemicellulase or the like is used may be used.

  On the other hand, dead cells of microorganisms used in the present invention are those obtained by killing microorganisms such as bacteria and yeasts that are not harmful to animals by methods such as sterilization, sterilization, crushing, autolysis, and enzymatic degradation. It is possible to use a yeast cell disruption product or a lactic acid bacteria killed cell body. As an example, in the case of yeast, yeast (Saccromyces cerevisiae) used for alcoholic brewing, bread production, or yeast such as Torula yeast (Candida utilis), such as autolysis, enzyme treatment, ultrasonic treatment, pressure, etc. The yeast cell disruption material which disrupted the cell by can be used. In addition, lactic acid bacteria killed cells may be those obtained by sterilizing cells such as lactic acid cocci (genus Enterococcus) or lactobacilli (genus Lactobacillus) by a method such as heating. These dead cells are known to stimulate the intestinal immunity system of domestic animals to activate immunity and increase the anti-disease properties of animals.

  The feed formulation of the present invention is obtained by mixing a predetermined amount of the above components. Any mixing method may be adopted as a mixing method.

  As content of the mannose and / or mannooligosaccharide contained in 1g of feed compounding agents of this invention, and the dead cell body of microorganisms, 0.001-0.9g is preferable, and mannose and / or mannooligosaccharide is 0.00. 02 to 0.8 g is more preferable, and 0.05 to 0.5 g is most preferable. The dead cell of the microorganism is preferably 0.05 to 0.99 g, more preferably 0.08 to 0.9 g, and most preferably 0.1 to 0.5.

  In addition, the feed additive of the present invention includes prebiotics such as oligosaccharides, fermentable organic acids, water-soluble dietary fibers, starches, and insoluble dietary fibers for the purpose of assisting intestinal regulation by mannose or mannooligosaccharides. Probiotics such as fungi may be added.

  Examples of viable bacteria include Lactobacillus genus such as Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus casei, Bifidobacterium thermophilum, Bifidobacterium pseudolongum, Bifidobacterium longum, etc. Bifidobacterium genus, Enterococcus faecalis, Enterococcus genus Enterococcus, Streptococcus thermophilus, Streptococcus mutans Streptococcus genus, Pediococcus genus (Leuconostoc) genus, Lactococcus, etc., live bacteria generally called lactic acid bacteria, or Clostridium Butyric acid bacteria such as Chirikamu (Clostridium genus), Bacillus subtilis (B. subtilis), Bacillus cereus, Bacillus badius, such as Bacillus (Bacillus) genus, such as Bacillus coagulans are commonly used as livestock feed.

  Furthermore, a stabilizer and an excipient may be included as necessary. Examples of the stabilizer include silicic acid anhydride, and examples of the excipient include bran, wheat flour, corn starch, corn gluten feed, dextrin, rice bran and the like.

  The 2nd animal feed of this invention mix | blends the above-mentioned feed compounding agent. The blending ratio is preferably 0.005 to 5% by weight, more preferably 0.02 to 2% by weight, and most preferably 0.1 to 2% by weight.

  Hereinafter, the present invention will be described specifically by way of examples. In addition, this invention is not limited to an Example.

Example 1
Enzyme-treated copra meal-derived mannose (trade name “Kocco Ace”, manufactured by Unitika Ltd.) 0.4 g, feed enzyme-treated yeast (trade name “Nutricermos”) 0.4 g, and wheat flour 0.2 g are mixed, The feed formulation of the present invention was prepared.

Example 2
Enzyme-treated copra meal-derived mannose (trade name “Kocco Ace”, manufactured by Unitika Ltd.) 0.4 g, lactococcus (enterococcus faecalis) killed bacteria 0.4 g, and wheat flour 0.2 g are mixed, and the feed composition of the present invention An agent was prepared.

Example 3
Yeast cell-derived mannose (trade name “Biomos”, manufactured by Oltech) 0.4 g, feed enzyme-treated yeast (trade name “Nutricermos”) 0.4 g, and wheat flour 0.2 g are mixed. A feed formulation was prepared.

Example 4
Yeast cell-derived mannose (trade name “Biomos”, manufactured by Oltech) 0.4 g, Lactococcus (Enterococcus faecalis) killed 0.4 g, and wheat flour 0.2 g are mixed to produce the feed formulation of the present invention. did.

Comparative Example 1
Yeast cell-derived mannose (trade name “Biomos”, manufactured by Oltech) 0.4 g and wheat flour 0.6 g were mixed to prepare a feed formulation.

Comparative Example 2
A feed compounding agent was prepared by mixing 0.4 g of feed enzyme-treated yeast (trade name “Nutricermos”) and 0.6 g of wheat flour.

Comparative Example 3
A feed formulation was prepared by mixing 0.4 g of killed Lactococcus (Enterococcus faecalis) and 0.6 g of wheat flour.

Test example 1
The feed composition prepared in Examples 1 to 4 and Comparative Examples 1 to 3 was added to commercially available artificial milk for piglets to prepare a blended feed. The feed compounding rate was 0.3%, and the artificial milk for piglets was changed according to the growth stage of the piglets. A group of 30 piglets were fed the prepared feed and reared from 5 to 70 days of age. Moreover, as a comparison, only commercially available artificial milk for piglets was fed without adding the feed formulation, and the animals were reared in the same manner (Comparative Example 4).

  The stool state from 5 days to 40 days of age during the breeding period of the domestic pigs was observed.

As a result of the above, compared to the piglets bred with the feed formulation prepared in the comparative example, the diarrhea occurrence days are smaller in the piglets bred with the feed formulation prepared in the example, that is, mannose It was also shown that the effect of preventing diarrhea in piglets is synergistically increased when mannooligosaccharide and dead microorganisms are combined.

  Table 2 shows the results of visual observation of the health condition of the piglets raised in Test Example 1 during the breeding period.

From the above results, compared to the comparative example, the piglet bred with the feed formulation prepared in the example is more vigorous and active, has good skin tone, and the stomach tension is also good. It was good and overall health was found to be good.

  Furthermore, Table 3 shows the results of comparing the average body weights of the pigs raised in Test Example 1 at the time of birth, 21 days of age, and 70 days of age.

From the above results, compared to the comparative example, the piglets bred with the feed formulation prepared in the examples were larger in both 21-day-old weight and 70-day-old weight, that is, death of mannose or mannooligosaccharide and microorganisms It was shown that the growth promotion effect of piglets is synergistically increased when the cells are combined.

Test example 2
The feed formulation prepared in Examples 1 and 3 and Comparative Examples 1 and 3 was added to commercially available calf artificial milk to prepare a feed. The addition rate of the feed formulation was 0.2%, and the feed was changed according to the growth stage of the calf. A group of three beef calves was fed with the prepared feed and raised from 1 month to 3 months of age. In addition, as a comparison, only commercially available artificial milk was fed without adding the feed formulation, and reared in the same manner (Comparative Example 5).

  The stool state of the calves bred in Test Example 2 was observed every day during the test period, and the stool score was recorded. The fecal score was 0 points for normal stool, 1 point for soft stool, 2 points for diarrhea, and 3 points for watery stool. Table 3 shows the average diarrhea days and the average stool score during the observation period.

As a result of the above, compared to the beef calf calf bred with the feed formulation prepared in the comparative example, the beef calf bred with the feed formulation prepared in the example has fewer days of diarrhea, The fecal condition was also good. That is, it was shown that when mannose or mannooligosaccharide was combined with killed microorganisms, the effect of preventing calf diarrhea synergistically increased.

As a result, it was found that feeding the livestock formulation of the present invention to livestock synergistically enhances the prevention of livestock diarrhea, the improvement of health, and the promotion of growth.

Claims (4)

A feed formulation comprising mannose and / or mannooligosaccharide and dead microorganisms as active ingredients. The feed formulation according to claim 1, wherein the mannose and / or mannooligosaccharide is a mannan degradation product. 3. The feed formulation according to claim 1 or 2, wherein the dead cells of microorganisms are yeast cell disrupted matter or lactic acid bacteria dead cells. An animal feed comprising the feed formulation according to any one of claims 1 to 3.