JP2006220531A - Biological sample discrimination plate - Google Patents

Abstract

PROBLEM TO BE SOLVED: To accurately detect detection results of a plurality of test items in a short time at the time of detection of a transport reaction when a biological sample is moved in a buffer filled in a channel, and is easy to handle. A small, lightweight and inexpensive biological sample discrimination plate is provided.
SOLUTION: A quantitative fractionation part for taking a certain amount of biological sample into the buffer flow path is provided at a confluence of a sample flow path through which a biological sample flows and a buffer flow path through which a buffer flows. A closed flow path that forms the buffer flow path in a biological sample determination plate that detects a transport reaction obtained by moving a captured biological sample in a buffer and determines the biological sample. The sample flow path is provided inside.
[Selection] Figure 2a

Description

  The present invention relates to a biological sample discriminating plate for discriminating a biological sample by moving DNA, protein or other biological sample in a buffer and detecting its transport reaction.

  When a general biological sample is considered, DNA and protein exist largely. In recent years, with the rapid development of molecular biology, the involvement of genes in various diseases has been understood fairly accurately, and attention has been focused on medical treatments targeting genes.

  With regard to DNA, SNPs (abbreviation of single nucleotide polymorphism, which is generally translated as “single nucleotide polymorphism” and is a general term for the difference of one code (one base) in a gene) are currently attracting attention. The reason for this is that the classification of SNPs can predict the prevalence of many diseases and the effects and susceptibility of each individual to drugs, and there are absolutely no humans on the planet who have the same SNPs, even if they are parents and siblings. This is because it is considered that the individual can be completely identified from not doing so.

  Currently, as a method for examining SNPs, sequencing (determination of base sequence) in which the base sequence of DNA is directly read from the end is most commonly used. There are several reports on the sequencing method, but the most common method is dideoxy sequencing (Sanger method). Sequencing is based on a technique that separates and identifies the difference in length of one base length by denaturing polyacrylamide gel electrophoresis with high resolution or capillary electrophoresis in any method including the Sanger method. This is true.

  Another method is affinity ligand capillary electrophoresis.

  Affinity ligand capillary electrophoresis uses intermolecular affinity, particularly specific affinity in the ecosystem (enzyme and substrate, antigen and antibody affinity, etc.) to give specificity to separation. Specifically, When an affinity ligand that specifically recognizes the base sequence is added to the electrophoresis solution in the capillary tube and the sample is electrophoresed, only the molecular species that interact in the sample mixture will change in the moving speed. The analysis is performed with attention (for example, see Patent Document 1).

  On the other hand, proteins exist in cells, tissues, and biological fluids, regulate biological activities, supply energy to cells, synthesize important substances, maintain biological structures, and communicate between cells and intracellular information. Involved in transmission. Currently, it has become clear that proteins have multiple functions depending on the various environments, the presence of other interacting proteins, and the degree and type of modification that the protein has undergone.

  Proteins are made by connecting 20 types of amino acids in order according to gene instructions (sequence information), and the types are said to be tens of millions. If you know the sequence of the gene, what amino acid is what? You can get information on whether they are connected in order. A complete set of proteins made from the genes (genomes) of organisms is called a proteome, and now the analysis of the proteome has been actively promoted after the nucleotide sequence of the human genome has been deciphered.

Such functional analysis of proteins requires not only identification and characterization, but also biochemical assays, protein-protein interaction studies, protein networks, and intracellular and extracellular signaling elucidation. Research on this protein function uses a variety of techniques, including enzyme assays, yeast two-hybrid assays, chromatographic purification, information tools and databases, etc. Protein discrimination by electrophoresis is particularly important. It is a technique. Then, as in electrophoresis, the transport reaction obtained when moving liquids such as samples, analytes, buffers, and reagents in capillary tubes is detected, and analysis, discrimination, determination, etc. of the samples are performed. There are various reports regarding the transportation and orientation of the liquid when it is performed (for example, Patent Documents 2 to 5).
JP 7-311198 A Special Table 2000-513813 JP-T-2001-523341 JP 2000-514928 gazette JP 2003-28883 A

  As described above, in the analysis of biological samples, a method using a capillary electrophoresis apparatus is widely used.

  In many cases, the capillary, which actually performs the transport reaction, uses a glass tube having an outer diameter of about 300 microns and an inner diameter of about 100 microns, and the surface is coated with polyimide or the like to make it difficult to break.

  However, in order to detect the internal sample, a part of the coating is peeled off by baking as a detection window or dissolving with chemicals.

  At this time, the part from which the coating has been peeled easily breaks, and it is necessary to be careful in handling. It is still dangerous if it breaks.

  In addition, sample injection is generally performed by pressurization or suction. However, it is necessary to inject a fixed amount, but the injection amount depends on changes in the viscosity and temperature of the buffer in the capillary, although it is adjusted over time. However, there is a problem that it is different for each experiment. The amount of sample has a great influence on the measurement result and is a very important item.

  In addition, in the case of an apparatus using such a capillary, it is difficult to perform electrophoresis in a short flow path because of the configuration, and the measurement takes a longer migration distance and time than necessary.

  Furthermore, in order to separate the sample even when the flow path on the plate is used, for example, in the methods described in Patent Documents 5 and 6, a plurality of cavities channels are crossed and at least three electrodes are provided, The sample is applied to two of the at least three electrodes, and the sample is moved through the intersecting portion. In this method, since the flow paths intersect, the sample is electrophoresed. There is a possibility that migration may not be performed well when performing the measurement, and an accurate measurement result cannot be obtained. For example, in the methods described in Patent Documents 3 and 4, the sample is moved by burying a fine channel in the platform and changing the rotational speed of the platform to change the centripetal force resulting from the rotation. In this method, in addition to the problem that the sample can be moved only in the centripetal direction, there is also a problem that the shape of the microchannel is considerably complicated.

  In the present invention, when a transport reaction is detected when a biological sample is moved in a buffer filled in a flow path, a plurality of test items can be accurately detected in a short time, and handling is easy. Another object is to provide a small, lightweight, and inexpensive biological sample discrimination plate.

  In order to solve the above-described problem, the biological sample discrimination plate of the present invention is configured such that a certain amount of biological sample is placed in a joint portion between a sample channel through which a biological sample flows and a buffer channel through which a buffer flows. A biological sample discriminating plate for determining a biological sample by detecting a transport reaction obtained when a biological sample taken into the quantitative fractionating unit is moved in a buffering agent, The sample channel is provided inside a closed channel forming the buffer channel.

  Furthermore, the length of the portion formed in the outer peripheral direction from the center of the biological sample discrimination plate of the buffer channel is longer than the length of the portion formed in the rotation direction.

  Further, the buffer channel is rectangular in shape.

  Further, the buffer channel is formed on a concentric circle whose center of rotation is the center of gravity of the biological sample discrimination plate.

  Furthermore, the number of concentric circles provided with the buffer flow path is two.

  Furthermore, the flow path cross-sectional area of the inner peripheral flow path is larger than the flow path cross-sectional area of the outer peripheral flow path.

  According to the biological sample discrimination plate of the present invention, since the area occupied by the flow path on the plate is small, the plate size can be further reduced. Furthermore, according to the biological sample discrimination plate of the present invention, since a large number of flow paths can be formed, a plurality of items can be inspected simultaneously.

(Embodiment 1)
Hereinafter, the biological sample discrimination plate according to the first embodiment will be described with reference to FIGS.

  The present invention realizes that biological samples can be easily and inexpensively and accurately distinguished in a short time by moving biological samples in a buffer and performing biological, enzymatic, immunological and chemical reactions. To do.

  In the first embodiment, for the sake of specific explanation, it is assumed that the biological sample is a DNA sample, and the buffering agent includes a separation DNA conjugate and a DNA binding control agent. The plate adds the quantified DNA sample to the separation DNA conjugate packed in the flow path, performs electrophoresis, detects the fluorescence or absorbance in the flow path, and detects the DNA sample. The presence or absence of SNPs (single nucleotide polymorphisms) shall be determined.

First, the configuration of the biological sample discrimination plate according to the first embodiment will be described with reference to FIGS. FIG. 1 is a diagram seen from the flow path forming surface of the biological sample discrimination plate according to the first embodiment.
FIG. 2a is a diagram showing a detailed shape of the outer peripheral side pattern 2 formed on the biological sample discrimination plate 1 shown in FIG.

  The biological sample discriminating plate 1 according to the first embodiment has 16 radially outer peripheral patterns 2 shown in FIG. 2a, and 8 radially inner patterns 2 'are radially formed. Thus, it is possible to discriminate DNA of 24 samples at the same time by the outer peripheral side pattern 2 and the inner peripheral side pattern 2 ′. The outer peripheral side pattern 2 and the inner peripheral side pattern 2 ′ are formed on concentric circles centered on the center of gravity of the biological sample discrimination plate 1.

  As shown in FIG. 1, the external shape of the biological sample discrimination plate 1 according to the first embodiment is an 8 cm square, three of the four corners are provided with R, and the remaining one is chamfered.

  Further, a hole 4 is provided, which is designed so that the pattern location can be specified by making the outer shape asymmetric. The material is acrylic resin and the thickness is 2 mm. Grooves are formed on the flow path forming surface, and a sealed flow path is formed by bonding an acrylic film having a thickness of 50 μm.

  The center of gravity 5 is the center of gravity of the biological sample discriminating plate, and a hole for fixing the biological sample discriminating plate to the rotating portion is provided around the center of gravity 5.

  The buffer injection port 6 shown in FIG. 2a is a buffer injection port for injecting a DNA conjugate as a buffer, and the buffer injection unit 7 is a buffer injection unit for temporarily holding the injected buffer, and a sample injection port. 8 is a sample injection port for injecting a DNA sample, and a sample injection unit 9 is a sample injection unit for temporarily holding the injected DNA sample. The buffer injection part and the sample injection part have the same shape, and a peripheral cross section is shown in FIG.

  FIG. 3 is a view showing a cross section in the vicinity of the buffer injection part and the sample injection part, and the hatched portion is the biological sample discrimination plate 1. 35 is the buffer injection port 6 and the sample injection port 8, and 36 is the buffer injection unit 7 and the sample injection unit 9. 37 is the above-described film, and a sealed flow path is formed by sticking the groove 24 so as to cover it. Incidentally, the flow path forming surface is the lower part.

  The positive electrode insertion portion 12 and the negative electrode insertion portion 13 are connected via a flow path 14, and are further connected to the buffer injection part by flow paths 10 and 11, respectively.

  The chamber part 16 is connected to the sample injection part 9 by a flow path 15.

  Reference numeral 20 denotes a sample holding portion, which is connected to the chamber portion 16 by the flow channel 17 and the flow channel 18, but the flow channel 17 is thin and the flow channel 18 is thicker than the flow channel 17. In the present embodiment, the width of the flow path 17 is 25 μm, and the flow path 18 is 50 μm. Moreover, the depth of the flow path is 30 μm. In the case of the inner peripheral pattern 2 ′ formed on the biological sample discrimination plate 1, the width of the channel 17 ′ is 40 μm, the channel 18 ′ is 80 μm, and the channel depth is all 50 μm. Yes.

Reference numeral 21 denotes a buffer unit, which is connected to the chamber unit 16 and the sample holding unit 20 by the channel 17, the channel 18, and the channel 19, respectively, and is further connected to the sample injection unit 9 and the buffer unit 21 by a channel 22 The buffer unit 21 can be vented.
The buffer injection part 7, the flow path 10, the flow path 11, the positive electrode part 12, the negative electrode part 13, and the flow path 14 are closed flow paths, and the buffer material flows through the closed flow paths, A buffer flow path is configured.

  As shown in the inner peripheral side pattern 2 ′ in FIG. 1, the pattern shape of the buffer channel is a rectangular shape arranged in the outer peripheral direction with respect to the center of the plate, and the channel length extending toward the outer periphery This length is longer than the flow path length extending in the rotation direction on the circumference. This is because many flow path patterns are formed on the concentric circles of the plate.

  Subsequently, the sample injection unit 9, the channel 15, the chamber unit 16, the channel 17, the channel 18, the channel 19, the sample holding unit 20, the buffer unit 21, and the channel 22 are also closed channels. The biological sample flows through the closed flow path to form a sample flow path. The buffer flow path and the sample flow path are joined by the sample quantitative sorting unit 23, and the DNA sample quantified by the sample quantitative sorting unit 23 is actually discriminated.

  Here, in the present embodiment, the sample channel is disposed inside the buffer channel. In order to increase the number of inspection items as much as possible, the space in the concentric direction of the flow path pattern was designed to be narrow. If the sample flow paths are arranged concentrically with the buffer flow paths, the space in the concentric direction of the flow path pattern becomes wider as shown in FIG. 2b. Compared to 24 channels, the number is 18 channels.

  The chamber part 16, the sample holding part 20, and the buffer part 21 have the same shape, and a sectional view of the periphery thereof is shown in FIG. In FIG. 4, the lower side is a flow path forming surface.

  The groove 24 is a portion corresponding to the chamber portion 16, the sample holding portion 20, and the buffer portion 21, and has a container shape having a depth of 1.5 mm when viewed from the flow path forming surface. 25 and 26 are flow paths extending from the respective parts.

  Next, the positive electrode portion 12 and the negative electrode portion 13 will be described with reference to FIG.

  FIG. 5 is a sectional view of the periphery of the positive electrode portion 12 and the negative electrode portion 13, and the lower side is a flow path forming surface.

  The hole 30 is a portion corresponding to the positive electrode portion 12 and the negative electrode portion 13 and is a hole penetrating the plate. Although the film 37 and the film 27 are attached to both surfaces, the film 37 may be nonconductive, whereas the film 27 may be conductive. In the case of conductivity, a voltage can be applied to the sample in the electrode section 30 by applying a voltage to the film 27 from the outside. In the case of non-conductivity, a voltage can be applied by inserting a needle-like electrode into the inside.

  Moreover, while the film 37 is affixed on the plate front surface, the film 27 is affixed only in the vicinity of the electrode portion.

  Now, an example of specific operations and operations up to the determination of the presence or absence of SNPs (single nucleotide polymorphisms) in the DNA sample will be described below with reference to FIG. 2 a showing the detailed shape of the outer peripheral side pattern 2. .

  First, a DNA sample as a specimen is prepared.

  Originally, the DNA has a double-stranded helical structure, but in Embodiment 1, a single-stranded DNA having a length of about 60 bases including the SNPs site to be discriminated is prepared.

  Since the extraction method and single strand formation are not directly related to the present invention, detailed description thereof is omitted.

  Next, a DNA conjugate is prepared as a buffer.

  The DNA conjugate is obtained by covalently bonding a high molecular linear polymer to the 5 'end of a 6 to 12 base long single-stranded DNA. Furthermore, DNA is a sequence that is complementary to the normal type but not complementary to the mutant type, has a strong binding force to the normal type DNA, and a weak binding force to the mutant type DNA. There are characteristics. In addition, when electrophoresed, the linear polymer bonded to the 5 'end has a characteristic that the migration speed is considerably slow.

The “DNA conjugate” described hereinafter has physical properties including a pH buffer that also serves as an electrolyte and a DNA binding force control agent such as MgCl ₂ .

  When sample preparation is complete, the DNA conjugate and DNA sample are injected into the plate. The DNA conjugate is dispensed from the buffer injection port 6 to the buffer injection unit 7 by a pipetter or the like. Similarly, the DNA sample is dispensed from the sample injection port 8 to the sample injection unit 9.

  Although the amount of dispensing differs depending on the scale of the pattern, in the first embodiment, the DNA conjugate is 3 microliters and the DNA sample is 1 microliter.

  Next, the biological sample discriminating plate 1 is fixed to a motor or the like, and rotated around the center of gravity 5. At this time, the dispensed DNA conjugate and the DNA sample move toward the outer circumference by centrifugal force.

  The DNA conjugate in the buffer injection part 7 passes through the flow path 10 and the flow path 11 and is equally divided into the positive electrode part 12 and the negative electrode part 13, and the DNA conjugate that has moved to the positive electrode part 12 further passes through the flow path 14. It moves to the fixed quantity fractionation part 23. Similarly, the DNA conjugate that has moved to the negative electrode portion 13 also moves to the quantitative sorting portion 23 through the flow path 14. FIG. 6 (a) shows a state where the movement of the DNA conjugate has stopped 2 minutes after the start of rotation. Further, FIG. 6B is an enlarged view of the periphery of the quantitative sorting unit 23.

  The DNA conjugate 31 is filled up to about 70% of the positive electrode portion 12 and the negative electrode portion 13, fills the flow path 14, and reaches the quantitative fractionation portion 23 as shown in FIG.

  The liquid level height of the DNA conjugate existing in the positive electrode part 12 and the negative electrode part 13 and the liquid level height of the sample quantitative fractionation part are on the same circumference with the center of gravity 5 as the center.

  Next, the movement of the injected DNA sample will be described with reference to FIG. 2a and FIG. The DNA sample in the sample injection section 9 passes through the flow path 15 and reaches the sample holding section 20 positioned on the outer peripheral side through the chamber section 16, the flow path 17 and the flow path 18. However, as shown in FIG. 7, the flow path 18 is connected on the outer peripheral side of the sample holding unit 20, and the sample holding unit 20 has no air vent hole. Therefore, the air remaining in the sample holding unit 20 does not escape and is in a pressurized state.

  FIG. 7 shows the state of the DNA sample 2 minutes after the start of rotation, and 32 and 33 are the DNA samples. Reference numeral 34 denotes compressed air, and the centrifugal force and the applied pressure are kept in an equilibrium state only during rotation. In this embodiment, the rotational speed is 4000 rpm.

  Next, the following operation will be described.

The biological sample discriminating plate 1 is rotated for a certain time, and the rotation is suddenly stopped in a state where the movement of the DNA conjugate and the DNA sample is stopped. (For example, stop from 4000 rpm in 2 seconds.)
Then, since the DNA sample 32 existing in the sample holding unit 20 has lost centrifugal force, it tends to flow backward from the sample holding unit 20 to the flow path 18 by the pressure of the air 34. Furthermore, the DNA sample 32 tries to move to the flow path 17 and the flow path 19 until the air 33 in the sample holding unit 20 reaches atmospheric pressure. At this time, the flow path 17 is formed narrower than the flow path 19. The DNA sample 32 tends to flow more into the channel 19 than to the channel 17.

  FIG. 8 shows a state immediately after the sudden stop.

  The DNA sample 32 in the sample holding unit 20 reaches the quantitative fractionation unit 23 and the buffer unit 21 through the flow paths 18 and 19 due to the expansion of the air 34.

  In particular, the quantitative sorting unit 23 comes into contact with the filled DNA conjugate 31.

  At this time, there are some DNA samples that move to the chamber portion 16 through the flow path 17.

  Next, the biological sample discriminating plate 1 is rotated again at a medium speed for several seconds. At this time, it is important to moderate the deceleration.

  FIG. 9 shows the state after the stop.

  Although the DNA sample filled in the flow path 19 flows to the chamber part 33, a small amount of DNA sample remains in the quantitative fractionation part 23. The remaining DNA sample is electrically insulated from other DNA samples.

  The reason why the operation is different from that in the first rotation is that the rotation speed is slow and the deceleration is slow, so that the air 34 in the sample holder 20 is not strongly compressed. Therefore, it does not flow backward and the channel 19 is not filled again.

  The sample DNA remaining in the quantitative sorting unit 23 by the above operation becomes the final sample for determining the SNPs. In addition, since the inner peripheral side pattern 2 ′ has a lower transport capacity due to centrifugal force than the outer peripheral side pattern 2, the difference in centrifugal force is obtained by taking the flow path cross-sectional area larger than the flow path cross-sectional area of the outer peripheral side pattern 2. Thus, it is possible to eliminate the difference in transport capacity, and as a result, the final sample for discriminating SNPs can be left in the quantitative sorting section in all patterns in the plate in one operation.

  Next, electrophoresis is performed. In the electrophoresis, a positive electrode is inserted into the positive electrode portion 12 and a negative electrode is inserted into the negative electrode portion 13, and a voltage of several hundred volts is applied. Then, an electric field is generated in the flow path 14 and further in the sample quantification part 23, and the DNA sample remaining in a certain amount in the quantification part 23 passes through the flow path 14 to the positive electrode side (A direction in FIG. 9). Run.

  The channel 14 is filled with a DNA conjugate, and the DNA sample is electrophoresed while repeatedly binding to the DNA conjugate. At this time, as described above, the normal DNA in the DNA sample has a strong binding force with the DNA conjugate, so the migration speed is slow, and the mutant DNA has a weak binding power, so the migration speed is faster than the normal type. That is, when both the normal type and the mutant type are present in the DNA sample, the normal type DNA and the mutant type DNA are separated, and SNPs can be discriminated.

  FIG. 11 is a graph obtained by scanning DNA during electrophoresis in a part 40 of the flow path 14 shown in FIG.

  The DNA was detected by exciting the DNA modified with a fluorescent label (FITC) with light of 470 nm and detecting light at around 520 nm. DNA may be detected by absorbance at 260 nm.

  The horizontal axis represents the position of the 40 portion, and DNA migrates from left to right. That is, the left side is the quantitative sorting unit 23 and the right side is the positive electrode unit 12 side. The vertical axis represents the fluorescence intensity, and represents a waveform that changes in time every minute from the top. You can see the two mountains gradually separating. In this case, it was determined that the same amount of normal DNA and mutant DNA were present in the DNA sample.

  The mountain on the right side of the graph is a mutant type because the migration speed is fast, and the left side is a normal type because it migrates slowly on the left side.

    As described above, according to the first embodiment, by providing the sample holding unit 20, a certain amount of DNA sample is held in the quantitative sorting unit 23 on the biological sample discrimination plate 1 only by the rotation operation, and the positive electrode The configuration is such that electrophoresis can be performed by the portion 12, the negative electrode portion 13, and the flow path 14. By forming the sample channel inside the buffer channel, the channel pattern can be made compact, and a plurality of items can be inspected with one plate. Furthermore, since a double flow path pattern can be inspected simultaneously by changing the cross-sectional area of the flow path, a plurality of items can be inspected.

  The biological sample discriminating plate of the present invention is useful for enabling discrimination of a biological sample such as a DNA sample at low cost and simply.

The figure which shows the groove | channel formation surface of the biological sample discrimination | determination plate concerning Embodiment 1 of this invention. The figure which shows the pattern formed in the biological sample discrimination | determination plate concerning Embodiment 1 of this invention. The figure which shows the surface where the groove | channel was formed in the flow path pattern which arranged the sample flow path and the buffer flow path in the concentric direction. Sectional drawing of the sample injection part of the biological sample discrimination | determination plate concerning Embodiment 1 of this invention, and a buffer injection part Sectional drawing of the chamber part, sample holding | maintenance part, and buffer part of the biological sample discrimination | determination plate concerning Embodiment 1 of this invention. Sectional drawing of the positive electrode part and negative electrode part of the biological sample discrimination | determination plate concerning Embodiment 1 of this invention. The figure which shows the state of a DNA conjugate when the sample filling process is given to the biological sample discrimination plate pattern concerning Embodiment 1 of this invention. FIG. 2 is an enlarged view of a quantitative fractionation section showing a state of a DNA conjugate when a sample filling process is performed on the biological sample discrimination plate pattern according to the first embodiment of the present invention. The figure which showed the state of each sample currently filled with the DNA conjugate and the DNA sample in the biological sample discrimination | determination plate concerning Embodiment 1 of this invention, and rotating at 4000 rpm. The figure which showed the state of each sample when a biological sample discrimination | determination plate concerning Embodiment 1 of this invention is filled with a DNA conjugate and a DNA sample, and it stops rapidly after rotating at 4000 rpm. The figure which showed the state of each sample immediately after re-rotating after filling the biological sample discrimination | determination plate concerning Embodiment 1 of this invention with a DNA conjugate and a DNA sample, and stopping rotation at 4000 rpm. The figure which showed the part which performs the DNA scan of the plate pattern for biological sample discrimination | determination concerning Embodiment 1 of this invention In the separation DNA conjugate in which the DNA sample is filled in the quantitative fractionation portion formed on the biological sample discrimination plate according to the first embodiment of the present invention, and the DNA sample is filled in the first channel. Figure showing how it moves

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Biological sample discrimination plate 2, 2 'Channel pattern 3 Rotation part fixing hole 4 Positioning hole 5 Plate gravity center 6 Buffer injection port 7 Buffer injection port 8 Sample injection port 9 Sample injection port 10 First flow channel 11 2nd flow path 12 Positive electrode part 13 Negative electrode part 14 3rd flow path 15 Flow path 16 Chamber part 17 Flow path 18 Flow path 19 Flow path 20 Sample holding part 21 Buffer part 22 Flow path 23 Fixed quantity fractionation part 24 Groove 25 Channel 26 Channel 27 Film 28 Channel 29 Channel 30 Hole 31 DNA conjugate 32 DNA conjugate 33 DNA conjugate 34 Air 40 Scan channel A Direction of migration

Claims (6)

A living body that has been taken into the quantitative sorting section by providing a quantitative sorting section for taking a certain amount of biological sample into the buffer flow path at the confluence of the sample flow path through which the biological sample flows and the buffer flow path through which the buffer flows. In a biological sample discrimination plate that detects a transport reaction obtained when a sample is moved in the buffer and discriminates the biological sample, inside the closed channel that forms the buffer channel A biological sample discriminating plate, comprising the sample flow path. 2. The biological sample discrimination plate according to claim 1, wherein the length of the portion of the buffer channel formed in the outer peripheral direction from the center of the biological sample discrimination plate is longer than the length of the portion formed in the rotation direction. A long and long biological sample discrimination plate. The biological sample discrimination plate according to claim 2, wherein the buffer channel has a rectangular shape. The biological sample discrimination plate according to claim 2, wherein the buffer channel is formed on a concentric circle with the center of gravity of the biological sample discrimination plate as the center of rotation. 5. The biological sample discriminating plate according to claim 4, wherein the number of concentric circles provided with the buffer channel is two. 6. The biological sample determination plate according to claim 5, wherein the flow path cross-sectional area of the inner peripheral flow path is larger than the flow path cross-sectional area of the outer peripheral flow path.

Images