JP2005330228A - Hair growth inhibitor, skin care preparation and cosmetic each compounded therewith - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a body hair growth inhibitor enabling body hair removing treatment to be mitigated, and to provide skin care preparations and cosmetics each compounded with the inhibitor. <P>SOLUTION: The body hair growth inhibitor contains Urtica thunbergiana Siebold et extract and Belamcanda chinensis D.C extract as active ingredients. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a hair growth inhibitor for body hair.

  In recent years, there is an increasing tendency that hair in hands, feet, heels and the like is preferable in terms of aesthetic appearance, and various hair removal methods have been developed and used. Specific examples include a mechanical removal method using a shaver, hair remover, etc., a method of removing hair from the hair root using a hair removal agent, and a method of removing body hair by its chemical action using a hair removal agent.

  However, these hair removal methods involve physical or chemical irritation to the skin, and the durability of the hair removal effect is limited although there are some differences depending on the hair removal method. For this reason, the hair removal process must be performed again after a certain period of time, and reduction of the hair removal process is desired.

From such a viewpoint, an inhibitor that suppresses hair growth has been developed, and as a patent application related to such an inhibitor, for example, an application as shown in Patent Document 1 below has been filed. The hair growth inhibitor described in Patent Document 1 contains pantothenic acid.
Japanese National Patent Publication No. 8-503220

  However, this Patent Document 1 does not clearly describe the action of pantothenic acid to suppress hair growth, and only discloses the test results obtained by weighing the amount of hair. Not sure.

On the other hand, an application such as the following Patent Document 2 has been made as a patent application relating to a hair growth inhibitor and a cosmetic containing the inhibitor. The hair growth inhibitor described in Patent Document 2 contains a flavonol glycoside as an active ingredient.
JP 2003-121727 A

This Patent Document 2 shows the effect of inhibiting growth by calculating the cell growth inhibition rate by a growth inhibition test of immortalized human outer root sheath cells. However, also in this patent document 2,
The effect of suppressing hair growth is not clear, and the effect is not always sufficient.

  The present invention has been made in order to solve such problems, and includes a hair growth inhibitor capable of reducing body hair removal treatment, and a skin external preparation and a cosmetic containing the hair growth inhibitor. The issue is to provide.

  There is a tissue called a hair papilla in the hair root of body hair, and it has recently been reported that the hair papilla produces various cytokines to control hair growth. Examples of cytokines that promote hair growth include HGF (hepatocyte growth factor), IGF-I (insulin-like growth factor-I), and TGF-β (transforming growth factor-β) as a cytokine that suppresses hair growth. )and so on. Therefore, it suppresses the growth of body hair by suppressing the production of cytokines that promote hair growth such as HGF produced by the hair papilla, or by promoting the production of cytokines that suppress hair growth such as TGF-β. It is considered possible.

  The present inventors pay attention to such points, and as a result of intensive studies on the hair growth inhibiting action and hair growth promoting action of various plant extracts, the nettle extract promotes the production of TGF-β by the hair papilla, The present invention has been completed by focusing on the combined use of these extracts with the ability to extract HGF by hair papilla.

  That is, the invention described in claim 1 is characterized in that the hair growth inhibitor contains the nettle extract and the cedar extract as active ingredients. Here, “contains nettle extract and capsicum extract as active ingredients” means that the hair growth inhibitor may consist of nettle extract and capsicum extract alone, and other than nettle extract and capsicum extract It means that the component of this may be included.

  The invention described in claim 2 is an invention for an external preparation for skin, wherein the hair growth inhibitor described in claim 1 is blended. Furthermore, invention of Claim 3 is invention of the cosmetics characterized by mix | blending the hair growth inhibitor composition of Claim 1.

  According to the present invention, it is possible to provide a hair growth inhibitor that has an excellent hair growth inhibitory effect and is highly safe for the human body, and an external preparation for skin and a cosmetic that contain the hair growth inhibitor. It was.

  Nettle (Urtica thubergiana Siebold et), one of the active ingredients in the hair growth inhibitor of the present invention, belongs to the nettle family and is a perennial plant distributed in Honshu, Shikoku and Kyushu. On the other hand, the other active ingredient, Higashigi (Belamcanda chinensis D.C), is a plant belonging to the family Iridaceae and the genus Hiogi.

  The extract used in the present invention is a variety of solvents obtained by extracting nettle and cedar pulverized products with a solvent at room temperature or under heating, or by using an extractor such as a Soxhlet extractor. It means the extract, its diluted solution, its concentrated solution, or its dry powder. The site of the plant (nettle, cypress) to be the extraction material is not particularly limited, but in the case of nettle, it is preferable to use leaves, and in the case of cypress, it is preferable to use rhizomes.

  As a solvent used for extraction, any solvent can be used as long as it is a solvent used for normal plant extraction. For example, water, alcohols such as methanol, ethanol, propylene glycol, 1,3-butylene glycol, glycerin, hydrous alcohols, organic solvents such as chloroform, dichloroethane, carbon tetrachloride, acetone, ethyl acetate, hexane, etc. They can be used alone or in combination.

  The external preparation for skin and cosmetics of the present invention containing the hair growth inhibitor as described above can be made into gel, lotion and other liquid forms, cream forms, aerosol forms, etc. It is not limited. As a skin external preparation, what is applied to a quasi drug, a pharmaceutical, etc. is used, for example.

  It is preferable that the compounding quantity of the nettle extract and the herb extract in the external preparation for skin and cosmetics of the present invention is usually 0.0001 to 50% by weight as a dry solid content. If the amount is less than 0.0001% by weight, the effect of the present invention may not be sufficiently obtained. On the other hand, if the amount exceeds 50% by weight, an improvement in the effect commensurate with the increase is not recognized. From this viewpoint, 0.001 to 10% by weight is particularly preferable.

  The skin external preparation and cosmetics of the present invention require various optional ingredients that are generally used in cosmetics or used in skin external preparations such as quasi-drugs and pharmaceuticals, as long as the effects of the present invention are not impaired. It can mix | blend suitably according to it. Examples of such optional components include purified water, ethanol, oily components, humectants, thickeners, preservatives, emulsifiers, medicinal components, powders, ultraviolet absorbers, dyes, fragrances, and emulsion stabilizers. be able to.

Examples of the present invention will be described below.
(Example 1)
The hair growth inhibitor of this example is composed of an extract component of nettle (Urtica thubergiana Siebold et) leaves and an extract component of rhizomes of bean (Belamcanda chinensis DC).

  One example of production will be described. 200 g of nettle leaves (dried material) are immersed in 2 L of a 50% ethanol solution, extracted at 60-80 ° C. for 2 days, the solvent is removed from the extract, and 15.8 g of An extract was obtained. Moreover, 200 g of rhinoceros rhizome (dried product) was immersed in 2 L of 50% ethanol solution and extracted at 60 to 80 ° C. for 2 days, and the solvent was removed from the extract to obtain 12.1 g of extract. The amount of the extract is shown as a value as a dry solid content.

(Test Example 1)
[Effect of human hair papilla cells on TGF-β production ability and HGF production inhibition ability]
Human hair papilla cells were seeded at 2 × 10 4 / well in a 12-well plate and cultured in Dulbecco's modified MEM medium (DMEM medium) supplemented with 10% fetal bovine serum (FBS) for 5 days. After washing the cells with DMEM medium on the 5th day of culture, the medium was replaced with DMEM containing 0.001% by weight of nettle extract and 0.001% by weight of cypress extract, and the culture was further continued.

After culturing for 3 days, the culture supernatant was collected, and the amount of TGF-β contained in the culture supernatant was measured by ELISA using a human TGF-β Immunoassay kit (manufactured by GT). The amount of HGF contained in the culture supernatant was measured by ELISA using a human HGF Immunoassay kit (manufactured by GT).
The effect of promoting the production of TGF-β and the suppression of the production of HGF by the extract of nettle extract and the extract of Hagigi were shown as values (ratio) when the amount of TGF-β and the amount of HGF were set to 100 when cultured in only DMEM medium. The results are shown in Table 1.

  As is clear from Table 1, the amount of TGF-β produced from human hair papilla cells cultured in a DMEM medium containing nettle extract is equal to the amount of human hair papilla cells cultured in a DMEM medium (control) containing no extract. Increased from the amount of TGF-β produced from. However, the amount of TGF-β produced from human dermal papilla cells cultured in a DMEM medium containing a barley extract was the same as that in the control. From this, it was recognized that the Hiragi extract promotes TGF-β production by hair papilla cells, but the nettle extract promotes TGF-β production by hair papilla cells.

  On the other hand, the amount of HGF produced from human hair papilla cells cultured in DMEM medium containing nettle extract was the same as the amount of HGF produced from human hair papilla cells cultured in control DMEM medium. The amount of HGF produced from human hair papilla cells cultured in the DMEM medium containing the product was significantly reduced as compared with the control. From this, it was recognized that the nettle extract does not suppress HGF production by hair papilla cells, but the barley extract suppresses HGF production by hair papilla cells.

(Test Example 2)
[Hair growth inhibition test using mice]
The back of C3H / He mice (8 weeks old, male) was shaved, and once a day, a 50% ethanol solution containing 0.01% by weight of nettle extract and 0.01% by weight of barley extract were used. A 50% ethanol solution containing 0.1% by weight of a 50% ethanol solution containing 0.01% by weight of a nettle extract and 0.01% by weight of a barley extract was applied for 20 days. On the other hand, the 50% ethanol solution not containing the above extract was similarly applied once a day to the back of C3H / He mice, and this was used as a control group. These hair growth inhibitory effects were examined by comparing the hair regeneration area ratio of the shaved portion on the 20th day after shaving. The results are shown in Table 2.

  As apparent from Table 2, the hair regeneration area in the control group was 88%, whereas C3H / He mice (nettle extract) coated with a 50% ethanol solution containing 0.01% by weight of nettle extract were used. In the product application group), the hair regeneration area was 39%, and in the C3H / He mice (application group of the cedar extract) coated with a 50% ethanol solution containing 0.01% by weight of the peony extract, the hair regrowth area was 52%. In both cases, the hair regeneration area was remarkably small compared to the control group. From this, it was recognized that both the nettle extract and the cedar extract have a very good inhibitory effect on mouse hair growth.

  Furthermore, in the C3H / He mice (nettle + peel extract application group) coated with a 50% ethanol solution containing both 0.01% by weight nettle extract and 0.01% by weight birch extract, the control group Needless to say, the hair regeneration area was narrower than that of the nettle extract application group and the cypress extract application group. From this, it was recognized that the inhibitory effect of mouse hair growth was further improved by the synergistic effect of the nettle extract and the cedar extract.

(Prescription Example 1)
This prescription example is a prescription example of a skin care lotion as an example of a cosmetic, and its composition is as follows.

Compounding ingredients Weight%
Ethanol 5.0
1,3-butylene glycol 7.0
Sorbit solution 10.0
Nettle extract 0.01
Hinogi extract 0.01
Methyl paraoxybenzoate 0.15
Citric acid 0.05
Sodium citrate 0.2
Edetate 0.05
Fragrance 0.1
POE hydrogenated castor oil 0.5
Purified water remaining

(Prescription example 2)
This prescription example is a prescription example of a skin care cream as an example of a cosmetic, and its composition is as follows.

Compounding ingredients Weight%
Cetanol 2.5
Squalane 10.0
Sara honey bee 1.0
Glyceryl trioctanoate 5.0
Octyldodecyl myristate 15.0
Tocopherol acetate 0.1
1,3-butylene glycol 7.0
Glycerol monostearate 3.0
POE (20) sorbitan monostearate 1.0
Sorbitan monostearate 2.0
Nettle extract 0.1
Hinogi extract 0.1
Concentrated glycerin 5.0
Butyl paraoxybenzoate 0.1
Methyl paraoxybenzoate 0.2
Purified water remaining

  The hair growth inhibitor of the present invention can be widely applied to external preparations for skin, cosmetics and the like.

Claims (3)

  A hair growth inhibitor comprising nettle extract and cypress extract as active ingredients.   An external preparation for skin, comprising the hair growth inhibitor according to claim 1.   A cosmetic comprising the hair growth inhibitor according to claim 1.