JP2005239984A - Antioxidant composition - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide an antioxidant composition which is useful for foods, cosmetics and medicines, can inhibit the generation of active oxygen, and can reduce the generated active oxygen. <P>SOLUTION: The antioxidant composition contains theanine, which inhibits the generation of active oxygen selected from the group consisting of O<SB>2</SB><SP>-</SP>, H<SB>2</SB>O<SB>2</SB>and HOCl or reduces the amount of the active oxygen. <P>COPYRIGHT: (C)2005,JPO&NCIPI

Description

  The present invention relates to an antioxidant composition (hereinafter sometimes simply referred to as a composition) characterized by containing theanine.

  In recent years, it has become clear that active oxygen plays an important role in living body defense systems, intracellular signal systems, etc., while causing various oxidative damages to biological components, causing aging and diseases. Yes.

The active oxygen, superoxide (· _{O 2} ^-), hydroxyl radical (HO ·), Hydroperoxyl (HOO ·), alkoxyl radicals (LO ·), alkyl peroxyl radical (LOO ·), nitric oxide ( Radicals such as NO), and hydrogen peroxide (H ₂ O ₂ ), singlet oxygen ( ¹ O ₂ ), peroxynitrite (ONOO ⁻ ), lipid hydroperoxide (LOOH), hypochlorous acid (HOCl), Non-radical species such as ozone (O ₃ ) can be mentioned. Of these, superoxide (· _{O 2} ^-), hydroxyl radical (HO ·), hydrogen peroxide _(H 2 _{O 2)} and singlet oxygen ⁽¹ _{O 2)} is called the narrow sense of active oxygen.

  Examples of the effects of these active oxygens on the living body include partial cleavage of DNA helix and tissue damage due to generation of chain radicals. As a result, allergic reactions such as bronchial asthma are induced; inflammation is induced by histamine release; ischemic heart diseases such as myocardial infarction and angina pectoris; exacerbation of diseases such as hepatic disorders such as fulminant hepatitis and fatty liver; Induction of dementia due to cerebrovascular disorders such as cerebral infarction due to active oxygen caused by excessive stimulation of various receptors by glutamate, which is important for signal transduction between cells, Alzheimer type dementia, Parkinson's disease, destruction of brain tissue, etc. It is known to contribute to diseases such as retinopathy of childhood, pulmonary emphysema, diabetes, gout, skin cancer, pregnancy toxemia, glomerulonephritis, diabetic nephritis, cataract, retinal iron rust, stains and wrinkles.

  Further, it is well known that active oxygen not only affects the living body as described above, but also triggers alteration or deterioration in food or the like, or accelerates alteration or deterioration.

  Therefore, suppressing the generation of active oxygen in the living body or food, or reducing the active oxygen in the living body or food is a point for treating or preventing these diseases, or suppressing or preventing the deterioration or deterioration of food. It is very important. For this reason, there is a substance (hereinafter referred to as an antioxidant substance) having an action (that is, an antioxidant action) that suppresses the generation of active oxygen in the living body or food or reduces the active oxygen generated in the living body or food. Search) has been widely conducted.

  Antioxidants include those derived from natural products such as fat-soluble tocopherol (vitamin E), carotenoids, water-soluble ascorbic acid (vitamin C), and synthetic compounds such as BHT (butylhydroxytoluene) and BHA. (Butylhydroxyaniline) and the like.

  Furthermore, attempts have been made to use superoxide desmutase (SOD) for the purpose of reducing active oxygen.

  In addition, it has been reported that effects such as prevention of skin aging and prevention of various diseases are expressed by using amino acids as antioxidants (see, for example, Patent Document 1).

Recently, many attempts have been made to obtain a substance having an antioxidative action from a herbal extract in search of sufficient medicinal efficacy and safety (for example, see Patent Documents 2 to 14). Also uses a crude drug or a plant-derived antioxidant substance.
International Publication No. 94/14755 Pamphlet JP-A-60-224629 JP-A 61-24522 Japanese Patent Laid-Open No. 2-193930 JP-A-2-243632 JP-A-2-264727 JP-A-3-153629 Japanese Patent Laid-Open No. 3-221588 JP-A-4-69343 JP-A-4-202138 JP-A-4-247010 JP-A-10-226787 JP 2000-129256 A JP 2002-308767 A

  However, the above-mentioned antioxidants are not sufficient in reducing active oxygen, and BHT and BHA are suspected to be carcinogenic, and neither of them is practical.

  In addition, since SOD is a protein, not only is it difficult to obtain it, but also there is a problem that it is digested by oral administration. Even when administered by injection to solve this problem, the half-life in blood However, it was not satisfactory as an antioxidant.

  Furthermore, since the above amino acids are not naturally occurring amino acids and are not approved for use as foods or food additives, they cannot be used in conventional foods.

  Therefore, the objective of this invention is providing the antioxidant composition which can be used as a foodstuff, cosmetics, and a pharmaceutical, can suppress generation | occurrence | production of active oxygen, and can reduce the generated active oxygen.

That is, the present invention
(1) an antioxidant composition comprising theanine,
(2) The antioxidant composition according to the above (1), which suppresses the generation of active oxygen selected from the group consisting of O ₂ ⁻ , H ₂ O ₂ and HOCl, or reduces the amount of the active oxygen.
(3) The antioxidant composition according to (1) or (2), which is a food or pharmaceutical product, and (4) the antioxidant composition according to (3), which is either a tablet or a capsule.

  According to the antioxidant composition of the present invention, there are effects that it is safe, the generation of active oxygen can be suppressed, and the generated active oxygen can be reduced. Furthermore, the antioxidant composition of the present invention can be used as foods, cosmetics and pharmaceuticals.

  The antioxidant composition of the present invention has one major characteristic in that it contains theanine.

  In the present invention, the term “antioxidation” means suppression of generation of active oxygen or reduction of active oxygen.

As the active oxygen, for example, superoxide (· _{O 2} ^-), hydroxyl radical (HO ·), Hydroperoxyl (HOO ·), alkoxyl radicals (LO ·), alkyl peroxyl radical (LOO ·), monoxide Radical species such as nitrogen (NO), and hydrogen peroxide (H ₂ O ₂ ), singlet oxygen ( ¹ O ₂ ), peroxynitrite (ONOO ⁻ ), lipid hydroperoxide (LOOH), hypochlorous acid (HOCl) ), Non-radical species such as ozone (O ₃ ). Of these, superoxide (· _{O 2} ^-), hydroxyl radical (HO ·), hydrogen peroxide _(H 2 _{O 2)} and singlet oxygen ⁽¹ _{O 2)} is called the narrow sense of active oxygen, the present invention It is an active oxygen that has a particularly remarkable antioxidant effect by the antioxidant composition.

  Expression of antioxidant action by theanine is, for example, iodine titration method, peroxide value measurement method such as rhodan iron method, decomposition product measurement method for measuring carbonyl value, TBA value, volatile substances, dissolved oxygen meter or Warburg meter A method of measuring oxygen consumption by using a gravimetric method, an ultraviolet absorption analysis, an infrared absorption analysis, an electron spin resonance analysis, a fluorescence analysis, a very weak emission analysis, or a biological system It can be evaluated by a method or the like.

  Theanine used in the present invention is a derivative of glutamic acid (γ-glutarethylamide), which is an amino acid component that is naturally contained in tea leaves. Examples of the method for producing theanine used in the present invention include extraction from tea leaves, organic synthesis (Chem. Pharm. Bull., 19 (7) 1301-1307 (1971)), a mixture of glutamine and ethylamine. A method for obtaining theanine by acting glutaminase (Japanese Patent Publication No. 7-55154), culturing a cultured cell group of tea in a medium containing ethylamine, and increasing the amount of accumulated theanine in the cultured cell group, A method for promoting growth (JP-A-5-123166), and replacement of ethylamine with an ethylamine derivative such as ethylamine hydrochloride in JP-B-7-55154 and JP-A-5-123166 provides L-theanine. There are methods, and any method may be used. Examples of the tea leaves include green tea leaves, oolong tea leaves, and black tea leaves.

  As for theanine, any of L-theanine, D-theanine, and DL-theanine can be used. Among them, the L-form is recognized as a food additive and is easily used economically. In L, the L-form is preferred. The form of theanine used may be any form such as a purified product, a crude product, or an extract. A commercially available product (Suntheanine (registered trademark) Taiyo Kagaku Co., Ltd.) may also be used.

  The content of theanine in the composition of the present invention is not particularly limited, and may be appropriately adjusted as desired. For example, the suitable theanine content in the composition is preferably 0.01 to 99.9% by weight, more preferably 0.1 to 99% by weight in consideration of expression of the desired effect of the present invention. is there.

  A method for detecting theanine in the composition of the present invention is not particularly limited, but high-performance liquid column chromatography (HPLC) using an ODS column after derivatization with orthophthalaldehyde (OPA) in a precolumn. ), And detection and quantification with a fluorescence detector, separation by HPLC using an ODS column, and detection and quantification at a wavelength of 210 nm.

  The composition of the present invention preferably further contains an active oxygen scavenger from the viewpoint of further enhancing the antioxidant effect. The content of the active oxygen scavenger in the composition of the present invention is not particularly limited, and may be appropriately adjusted as desired. For example, the suitable active oxygen scavenger content in the composition is preferably 0.01 to 99.9% by weight, more preferably 0.1 to 99% by weight in consideration of the expression of the desired effect of the present invention. %. The active oxygen scavenger is a substance having the property of removing active oxygen such as superoxycide, hydrogen peroxide, hydroxy radical, singlet oxygen, etc., for example, catechins, tannins, anthocyanins, flavonoids, saponins And each of these related components. You may use these individually or in mixture of 2 or more types. Many of these active oxygen scavengers have been confirmed to have a scavenger action or an antioxidant action, although they are weak.

  Although it does not specifically limit as catechin, Catechin, catechin gallate, epicatechin, epicatechin gallate, epigallocatechin, epigallocatechin gallate, gallocatechin, gallocatechin gallate etc. are mentioned.

  The tannins are roughly classified into hydrolyzable tannins and condensed tannins. Hydrolyzable tannin refers to tannin that generates gallic acid or ellagic acid by hydrolysis, and is not particularly limited, but includes gallotannin, ellagitannin, theogarin, chlorogenic acid, neochlorogenic acid, etc. Type tannin refers to tannin obtained by condensation polymerization of catechins having a flavan-3-ol skeleton, and is not particularly limited, and examples thereof include proanthocyanidins such as procyanidins and prodelphinidins.

  The anthocyanins are not particularly limited, but non-acylated anthocyanins, aliphatic organic acylated anthocyanins as acylated anthocyanins, monoacylated anthocyanins, polyacylated anthocyanins, co-pigmented anthocyanins, metallo Examples include anthocyanins, hordemins, and red wine pigments.

  Flavonoids include, but are not limited to, flavones, flavonols, flavanones, flavanols, isoflavones and the like.

  The saponins are roughly classified into triterpenoid saponins and steroid saponins. The triterpenoid saponin is not particularly limited, and examples thereof include oleanan saponin contained in kyoto, maroni, cyclamen, and damaran saponin contained in medicinal carrot. The steroidal saponin is not particularly limited, and examples thereof include diosin contained in yam, thymosaponin A-III contained in timo, offiopogonin A contained in bacmond, digitonin contained in digitalis, and the like.

  These active oxygen scavengers are included in herbal medicines, folk medicines, herbs, plant foods (eg beans, grains, vegetables, fruits, etc.), animal feeds (grass, straw, etc.), and so on. What was extracted from the raw material / raw material using a known method may be used, or the raw material / raw material may be used as they are. These materials and raw materials may be used alone or in combination of two or more.

  Herbal medicines are not particularly limited, but valerian, toki, glaze, peony, ginseng, kyokyo (root), licorice (rhizome), senega (root) kiraya (bark), jikitarisu (leaf), onidokoroko (Rhizome), salsa (root), chimo (flower root). The form is not particularly limited, and examples thereof include extracts and dried products.

  Examples of the folk medicine include, but are not limited to, turmeric, sweet tea, fig, chamomile, saffron, carrot, and coconut.

  The herbs are not particularly limited, but anise, carrot seed, clove, coriander, cypress, cinnamon, juniper, ginger, sweet orange, pine needle, basil, patchouli, bitter orange, fennel, black pepper, bay, Peppermint, Bergamot, Mandarin, Myrrh, Lemongrass, Rosemary, Grapefruit, Cedarwood, Citronella, Sage, Thyme, Tea Tree, Violet Leaf, Vanilla, Hyssop, Eucalyptus, Lime, Lemon, Ylang Ylang, Cardamom, Clarisage, Jasmine, Geranium , Chamomile, Bulgarian rose, rose, olivenum, lavender, chamomile, geranium, sandalwood neroli, verbena, petit glen, vetiver, ma Joramu, Melissa, rosewood, St. John's wort, Thane Tojonzuwato, and the like Kawakawa. Although the form is not specifically limited, Extract extract, essential oil, herbal tea, etc. are mentioned.

  Plant foods are not particularly limited, but include dice, azuki bean, green beans, spinach, sugar beet, Chinese yam, edible lily, leek, leek, radicchi, garlic, yuzu, rakanka, grape, carrot, tomato and onion. , Guava, and cranberries.

  Examples of animal feed include, but are not limited to, cereals such as grass, corn, grain sorghum, and wheat, sucrose such as bran and nutca, and vegetable oil.

  The composition of the present invention may further contain amino acids, vitamins, minerals, and other materials and raw materials acceptable for food. You may use these individually or in mixture of 2 or more types.

  Examples of amino acids include, but are not limited to, glutamine, glutamic acid, inosinic acid, alanine, arginine, aspartic acid, threonine, serine, γ-aminobutyric acid, taurine, thiotaurine, and hypotaurine.

The vitamin is not particularly limited, but vitamin A, vitamin B ₁ , vitamin B ₂ , vitamin B ₆ , vitamin B ₁₂ , vitamin C, vitamin D, vitamin E, vitamin K, folic acid, nicotinic acid, lipo Examples thereof include acids, pantothenic acid, biotin, ubiquinone, prostaglandins, and derivatives thereof.

  Although it does not specifically limit as a mineral, Calcium, iron, magnesium, copper, zinc, selenium, potassium etc. are mentioned.

  Other ingredients that are acceptable for food include aloe, royal jelly, melatonin, placenta, propolis, isoflavone, soy lecithin, egg yolk lecithin, egg yolk oil, chondroitin, cacao mass, collagen, vinegar, chlorella, spirulina, ginkgo biloba, green tea, Tochu tea, Huangcha tea, oolong tea, mulberry leaves, persimmon tea, banaba tea, unsaturated fatty acids, sugars such as sugar alcohol and oligosaccharide, fungi such as bifidobacteria and red yeast rice, agaricus persimmon, princess matsutake, ganoderma, maitake Mushrooms such as, blueberries, prunes, grapes, olives, fruits such as ume and citrus fruits, peanuts, almonds, sesame seeds such as sesame and pepper, peppers, chili, leeks, pumpkins, cucumbers, carrots, burdock, morohaya, garlic, Vegetables such as perilla, wasabi, tomato, raccoon, leafy vegetables, persimmons and beans Seaweeds such as wakame, seafood, Shishitorikujira meat, cereals etc., per se or, these extracts, dried products, crude products, purified products, processed products, and the like brewing product.

  Moreover, as a composition of this invention, a foodstuff or a pharmaceutical is preferable from a viewpoint that it is suitable for daily use.

  The food in the present invention includes not only food containing theanine but also food additives containing theanine.

  Examples of foods included in the present invention include solid foods such as dried foods and supplements, and liquid foods such as soft drinks, mineral water, taste drinks, and alcoholic drinks. Examples of the solid food include, but are not limited to, kneaded products, processed soybean products, seasonings, mousses, jelly, frozen desserts, strawberries, chocolate, gum, crackers, cakes, breads and the like. The liquid food is not particularly limited, but teas such as green tea, oolong tea, black tea, herbal tea, concentrated fruit juice, concentrated reduced juice, straight juice, fruit mixed juice, fruit juice with fruit juice, beverage with fruit juice , Fruit / vegetable mixed juice, vegetable juice, carbonated drink, soft drink, milk drink, sake, beer, wine, cocktail, shochu, whiskey, etc.

  In addition, the pharmaceuticals included in the present invention are not particularly limited as long as they contain theanine. For example, the form may be any of a solution, suspension, powder, solid molding, etc., and the dosage form is a tablet, capsule, powder, granule, drink, injection, patch, Suppositories, inhalants and the like can be mentioned.

  The production method of the composition of the present invention is not particularly limited. For example, a production method in which theanine and other raw materials are powder mixed, a production method in which theanine and other raw materials are dissolved in a solvent to obtain a mixed solution, and mixing thereof General food or pharmaceutical production methods such as a method of freeze-drying a solution and a method of spray-drying can be applied.

  For example, for the existing food, theanine is formulated by a conventional method so that the content of theanine in the food of the present invention after production is preferably within the suitable content range of theanine in the food. Food can be produced. In addition, the theanine is preferably used in the same preferred range of theanine content as in the case of food production, for example, with known organic or inorganic carriers, excipients, binders, stabilizers and the like suitable for oral administration. Thus, the pharmaceutical of the present invention can be produced by blending theanine by a conventional method.

The composition of the present invention produced as described above has an antioxidant effect against active oxygen, particularly O ₂ ⁻ , H ₂ O ₂ or HOCl.

  The dose of the composition of the present invention for obtaining the desired effect of the present invention is usually preferably 1 to 1000 mg / day, more preferably 5 to 500 mg / day as theanine. However, since there are individual differences (symptom type, degree, age, etc.), the dosage in the present invention is not limited to such a range, and individual specifics can be obtained so as to obtain the desired effect of the present invention. The dose may be set appropriately.

  The administration method of the composition of the present invention is not particularly limited, but is preferably oral administration, intravenous administration, rectal administration, more preferably oral administration, and may be administered within the above-mentioned preferred range.

  Theanine used in the present invention is highly safe. For example, in an acute toxicity test using mice, there is no death due to oral administration of 5 g / kg, and no abnormality is observed in the general state and body weight. In particular, L-theanine is known as a umami component of tea and is also used as a food additive for the purpose of taste, and there is no limit to the amount of addition in the Food Sanitation Law. Moreover, unlike the conventional drugs, no side effects due to theanine are observed, and therefore the present invention provides a composition having a safe and effective antioxidant action.

  EXAMPLES Hereinafter, although an Example and a test example demonstrate this invention in detail, this invention is not limited to the said Example and test example.

Production Example 1 Production of L-Theanine by Enzymatic Method 0.3 M glutamine and 1.5 M ethylamine hydrochloride in 0.05 M borate buffer (Na ₂ B ₄ O ₇ -NaOH, pH 11) in the presence of 0.3 U glutaminase And reacted at 30 ° C. for 22 hours to obtain 225 nmol of theanine. Next, purification of theanine from the reaction solution was performed by subjecting the reaction solution to chromatography using a Dowex 50 × 8 column and a Dowex 1 × 2 column (both manufactured by Muromachi Chemical Co., Ltd.), followed by ethanol treatment.

  First, L-theanine is confirmed by subjecting the isolated substance obtained by the purification to an amino acid analyzer (manufactured by Hitachi, Ltd.) and paper chromatography, and confirming that the isolated substance exhibits the same behavior as the standard substance. went. Subsequent hydrolysis of the isolated material with hydrochloric acid or glutaminase yielded glutamic acid and ethylamine in a 1: 1 ratio. Thus, since the isolated substance was hydrolyzed by glutaminase, it was shown that ethylamine was bonded to the γ-position of glutamic acid. It was also confirmed by glutamate dehydrogenase that glutamic acid generated by hydrolysis was L-form. From the above, it was finally confirmed that the obtained compound was L-theanine. At this time, L-theanine obtained was 8.5 g.

Production Example 2 Extraction of L-theanine from tea leaves After extraction of 10 kg of tea (Camellia sinensis) leaves with hot water, the obtained extract was applied to a cation exchange resin (Muromachi Chemical Co., Ltd .; Dowex HCR W-2). Eluted with NaOH. The eluted fraction was passed through activated carbon (Nimura Chemical Co., Ltd .; Dazai activated carbon SG), and the eluted fraction with 15% ethanol was concentrated using an RO membrane (Nitto Denko Corporation; NTR 729 HF). Subsequently, the concentrate was purified by column chromatography and recrystallized to obtain 24.8 g of L-theanine.

  In the following examples, Santheanine (registered trademark) (manufactured by Taiyo Chemical Co., Ltd.) was used as theanine.

Antioxidant Evaluation Method Antioxidant effect of the composition of the present invention is measured by measuring chemiluminescence from a luminescent substance generated by active oxygen using a chemiluminescence analyzer (CLA-SP2, manufactured by Tohoku Electronics Industrial Co., Ltd.). evaluated. The detection wavelength range was set to 400-870 nm, and the resolution was set to 4 nm in the band wavelength between 270 and 870 nm.

Example 1 Antioxidant Effect of Theanine on Superoxide (O ₂ ⁻ ) Theanine was dissolved in 50 mM phosphate buffer solution (pH 7.4) to a final concentration of 0, 1, 10, and 100 mg / ml. Prepared. 200 μl of the obtained sample was added to a sample chamber of a chemiluminescence analyzer (CLA-SP2, manufactured by Tohoku Electronics Industrial Co., Ltd.), and 100 μl of 0.1 mM lucigenin as a luminescent substance was added to the sample chamber 100 seconds after the sample was added. Then, 150 seconds after sample addition, 500 μl of 0.1 mM xanthine and 50 μl of 0.004 U xanthine oxidase were added to the sample chamber. The results of measuring chemiluminescence during this series of operations are shown in FIG.

  Next, samples were prepared by mixing theanine 0, 100, 150, 200 and 300 mg with 200 μl of 50 mM phosphate buffered saline (pH 7.4). The obtained sample was added to a sample chamber of a chemiluminescence analyzer (CLA-SP2, manufactured by Tohoku Electronics Industrial Co., Ltd.), and 100 μl of 0.1 mM lucigenin as a luminescent substance was added to the sample chamber 100 seconds after the sample was added. Then, 150 seconds after sample addition, 500 μl of 0.1 mM xanthine and 50 μl of 0.004 U xanthine oxidase were added to the sample chamber. Chemiluminescence was measured 150 seconds after addition of xanthine and xanthine oxygenase, and the results are shown in FIG.

From FIG. 1, the chemiluminescence count decreases depending on the concentration of theanine as compared to the blank containing no theanine, and the antioxidant effect of theanine against superoxide (O ₂ ⁻ ) is observed. From FIG. 2, the chemiluminescence count is significantly decreased with respect to the blank by adding 100 mg or more of theanine (p <0.05), and the antioxidant action of theanine against superoxide (O ₂ ⁻ ) is observed.

Example 2 Antioxidant Effect of Theanine on Hypochlorous Acid (HOCl) Theanine 0, 10, 50, 100, 150, 200 and 300 mg were mixed with 200 μl of 50 mM phosphate buffered saline (pH 7.4) to prepare a sample. Prepared. The obtained sample was added to a sample chamber of a chemiluminescence analyzer (CLA-SP2, manufactured by Tohoku Electronics Industrial Co., Ltd.), and 100 μl after adding the sample, 500 μl of 25 μM luminol was added to the sample chamber. Then, 150 seconds after sample addition, 100 μl of 0.12 wt% hypochlorous acid was added to the sample chamber. The chemiluminescence after 150 seconds of hypochlorous acid addition was measured, and the result is shown in FIG.

  The chemiluminescence count is significantly decreased with respect to the blank by the addition of theanine (p <0.05), and the antioxidant effect of theanine on hypochlorous acid (HOCl) is observed.

Example 3 Antioxidant Effect of Theanine on Hydrogen Peroxide (H ₂ O ₂ ) Samples were prepared by mixing 0, 200 mg and 300 mg of theanine with 200 μl of 50 mM phosphate buffered saline (pH 7.4). The obtained sample was added to a sample chamber of a chemiluminescence analyzer (CLA-SP2, manufactured by Tohoku Electronics Industrial Co., Ltd.), and 100 μl after adding the sample, 500 μl of 25 μM luminol was added to the sample chamber. Then, 150 wt.% Hydrogen peroxide (H ₂ O ₂ ) was added to the sample chamber 150 seconds after sample addition. The chemiluminescence after 150 seconds of hydrogen peroxide addition was measured, and the results are shown in FIG.

The chemiluminescence count is significantly decreased with respect to the blank by addition of theanine (p <0.05), and the antioxidant effect of theanine on hydrogen peroxide (H ₂ O ₂ ) is observed.

Example 4 Production of Theanine-Containing Tablet As an example of the theanine-containing antioxidant composition, the following raw materials were mixed, and tableted to 1 tablet 0.75 g after granulation to produce a theanine-containing tablet.
Frost sugar 71.67% by weight (0.5375 g)
Trehalose 10.00% by weight (0.0750g)
Theanine 13.33% by weight (0.1000 g)
Sucrose fatty acid ester 1.00% by weight (0.0075 g)
Fragrance (Takasago Fragrance Industry Co., Ltd.) 4.00% by weight (0.0300 g)
Total 100% by weight (0.7500g)

Example 5 Production of a theanine-containing candy A theanine solution was prepared by dissolving theanine (10 kg) in 10 kg of water. Next, granulated sugar (64 kg) was heated to 110 ° C. while dissolving in 20 kg of water, and theanine solution and chickenpox were added to raise the temperature to 145 ° C. Next, the fire was turned off, and 50 wt% tartaric acid (1 kg) and flavor (1 kg, manufactured by Takasago Fragrance Industry Co., Ltd.) were added and mixed. It cooled to 75-80 degreeC, it shape | molded with the shaping | molding roller, and theanine containing candy was manufactured as an example of the theanine containing antioxidant composition. In addition, as a result of measuring content of theanine in a candy, content was 89.6 mg / g at 1.2 g per 1 piece.

Example 6 Production of theanine-containing blueberry beverage Fructose glucose (12 kg), blueberry concentrated fruit juice (1 kg), 1/5 clear lemon juice (0.4 kg), Na citrate (0.05 kg) and theanine (0.1 kg) Add to water and dissolve with stirring. After adjusting the pH to 3.1 using 50 wt% Na citrate (crystal) and raising the temperature to 95 ° C., fragrance (0.05 kg, manufactured by Takasago Fragrance Co., Ltd.) was added, and water was added to a total amount of 100 kg. The can was filled into a can of 100 g and then sterilized and cooled to produce a theanine-containing blueberry drink as an example of the theanine-containing antioxidant composition. In addition, as a result of measuring the theanine content in a blueberry drink, content was 98.3 mg / 100g.

Example 7 Production of a theanine-containing grapefruit beverage Fructose glucose solution (6 kg), theanine (0.1 kg), ferric pyrophosphate (0.06 kg), placenta extract (0.01 kg) and grapefruit juice 100% (30 kg) Add to water and dissolve with stirring. After adjusting the pH to 3.1 using Na citrate and raising the temperature to 95 ° C., a fragrance (0.05 kg, manufactured by Takasago Fragrance Industry Co., Ltd.) was added, and water was added to a total amount of 100 kg. The can was filled into a can of 100 g and then sterilized and cooled to produce a theanine-containing grapefruit beverage as an example of the theanine-containing antioxidant composition. In addition, as a result of measuring the theanine content in a grapefruit drink, content was 96.4 mg / 100g. 100 ml each was filled and cooled to produce a theanine-containing grapefruit beverage.

  Since the antioxidant composition of the present invention is safe and has an effective antioxidant action, it can be used as food, cosmetics and pharmaceuticals.

FIG. 1 shows the antioxidant effect of theanine on superoxide (O ₂ ⁻ ). FIG. 2 shows the antioxidant effect of theanine on superoxide (O ₂ ⁻ ). FIG. 3 shows the antioxidant effect of theanine on hydroxy radical (HOCl). FIG. 4 shows the antioxidant effect of theanine on hydrogen peroxide (H ₂ O ₂ ).

Claims (4)

An antioxidant composition comprising theanine. O _{2 ^-,} H 2 O 2 and suppressing the occurrence of selected active oxygen from the group consisting of HOCl, or antioxidant composition according to claim 1, wherein for reducing the amount of active oxygen. The antioxidant composition according to claim 1 or 2, which is a food or a medicine. The antioxidant composition according to claim 3, which is either a tablet or a capsule.

Images