JP2001061370A - Aquatic life based on microorganism-culture solution, activating agent for hydrosphere for breeding it, activation of aquatic life by using the same and breeding of aquatic life - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an activating agent capable of activating aquatic life in an environment notwithstanding that it is a closed environment such as an aquarium or an open type environment e.g. installed in the sea, etc., and also maintaining a hydrosphere which is a living zone of such aquatic life at a constant state, and a method for doing it. SOLUTION: This activating agent is based on a microorganism and its metabolite capable of activating an aquatic life and optimizing a hydrosphere for bleeding the aquatic life, and provided by pouring a microorganism culture solution in which (a) aerobic microorganisms, (b) anaerobic microorganisms, (c) at least one kind of Basidiomycetes belonging to the genus Pleurotus and (d) at least photosynthetic bacteria containing green bacteria as essential components are living together, also containing enzymes derived from these microorganisms and carbon-decomposing enzyme, as the activating agent into the aquatic life and its hydrosphere. Thereby, it is possible to activate the exhausted aquatic life and also treat for cleaning green algae, algae, excrements, seston, etc., presenting in the hydrosphere of the aquatic life. Also in the hydrosphere cleaned by such treatment, it becomes possible to breed the aquatic life. Especially even in a closed system, it becomes possible to breed small fishes, etc., for a long period.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to (a) an aerobic microorganism group, (b) an anaerobic microorganism group, (c) a basidiomycete belonging to at least one kind of Pleurotus, and (d) a photosynthetic fungus. And a microorganism culture solution containing an enzyme derived from these microorganism group metabolites and a carbon-decomposing enzyme (hereinafter abbreviated as OME)
The present invention relates to a use for activating an aquatic organism and at the same time improving a hydrosphere as its living environment.

[0002]

2. Description of the Related Art In recent years, at homes, aquaculture companies, aquariums, and the like,
Ornamental fish such as goldfish, carp, tropical fish, tropical crabs, shellfish for watching shrimp, etc., cultivation and rearing of shellfish, etc., edible fish,
In aquaculture of crustaceans and the like, so-called fish cages and aquariums are used. Hereinafter, in the present invention, the aqueous organisms provided for such breeding are referred to as aqueous organisms. When these aquatic organisms are bred for a long time in a cage or an aquarium, depending on the breeding environment, the aquatic environment, which is a living environment, may be significantly contaminated by the discharge of aquatic organisms and the propagation of algae. Also, in open environments such as fishing ponds and fish farms, the breeding environment of aquatic organisms is significantly impaired by the occurrence of blue-green algae and sestons due to eutrophication of the hydrosphere, and the inflow of chemical hazards and the like. There are cases. When breeding aquatic organisms in a closed environment such as an aquarium, the water must be changed frequently or algae attached to the aquarium, indoor cages, etc. must be maintained in order to maintain the water quality in the aquatic area. Had to be washed frequently.

[0003] In addition, many aquatic organisms such as fish for appreciation are expensive and are often imported from distant places, especially from overseas, by ship or airplane. Aquatic organisms can be significantly degraded due to environmental changes and care must be taken in their transport. Therefore, there is a demand for activating these weakened aquatic organisms. Also, in recent years, the value of aquatic plants for appreciation has changed, and there is a demand for raising aquatic organisms in various environments.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to simultaneously activate these aqueous organisms regardless of the closed environment such as a water tank or the open environment provided in the sea or the like. It is an object of the present invention to provide an activator and a method capable of maintaining a constant state of the hydrosphere, which is the living area of these aquatic organisms. Another object of the present invention is to provide a method for easily breeding a wide variety of aquatic organisms even in a closed environment.

[0005]

Means for Solving the Problems The present inventors have made intensive studies in view of the above problems and found that (a) a group of aerobic microorganisms,
(B) anaerobic microorganisms, (c) basidiomycetes belonging to at least one kind of Pleurotus, (d) photosynthetic fungi containing green bacteria as an essential component, and enzymes derived from metabolites of these microorganisms The use of a microbial culture solution characterized by containing a microbial culture solution characterized by the fact that it contains carbon-degrading enzymes not only purifies the hydrosphere in which aquatic organisms grow, but also activates the aquatic organisms themselves. The inventors have found that the present invention can be used, and have led to the creation of the present invention.

That is, the present invention relates to an activator based on a microorganism and a metabolite thereof that activates an aqueous organism and optimizes the aquatic area in which the aqueous organism is bred, and (a) a group of aerobic microorganisms, b) anaerobic microorganisms, (c) basidiomycetes belonging to at least one kind of Pleurotus, (d) photosynthetic fungi containing green bacteria as an essential component, and enzymes derived from metabolites of these microorganisms. The present invention relates to an activator, comprising a microbial culture solution containing a carbon-decomposing enzyme.

[0007] The present invention is also a method for optimizing the aquatic zone for breeding the aquatic organisms while activating the aquatic organisms, wherein the activator is introduced into the aquatic area where the aquatic organisms live. And purifying the hydrosphere and activating aqueous organisms. The method is applicable to a hydrosphere contaminated by eutrophication or a closed hydrosphere. Further, in the above method, it is preferable to add 0.01 to 1.0 cc of the activator to 1 liter of hydrosphere. The term "activate aquatic organisms" used in the present invention means activating aquatic animals that have been consumed or weakened due to contamination of the aquatic area, high population density, long-term transportation, and the like.

[0008] The present invention further relates to a method for activating an aqueous organism, wherein the activator is applied to a target aqueous organism. Furthermore, the present invention relates to a method for breeding an aqueous organism, which comprises introducing the active agent into the hydrosphere of the aqueous organism to be bred. According to this method, it is possible to breed an aqueous organism in a closed container or an accessory such as a pendant.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION Hereinafter, the present invention will be described in detail.
Aqueous organisms that are the subject of the present invention are not particularly limited as long as they live in the water and are bred or cultivated by humans, and appreciable and edible freshwater or marine fish,
Crustaceans and shellfish are included. In addition, the hydrosphere in the present invention may be used for homes and aquaculture companies, for transportation, in canteens, and in closed systems such as aquariums such as aquariums, but also for fish cages installed in the sea, rivers, lakes and marshes. It may be an open system. In the present invention, an activator based on a microorganism and a metabolite thereof that activates the aquatic organism and optimizes the hydrosphere in which the aquatic organism is bred refers to an aquatic organism, for example, long-term transport, an excessively high individual density, and water quality. It has the ability to activate aquatic organisms that have been depleted or weakened due to various reasons such as pollution, and removes algae contained in the aquatic area where the aquatic organisms inhabit, such as blue-green algae and algae attached to the aquarium. And has the ability to improve the environment unfavorable for the propagation of aquatic organisms such as effluents of aqueous organisms and chloride ions contained in water. Specifically, the PCT / JP99 / 1234
The base is OME or a diluent thereof as described in detail in the specification of US Pat.

[0010] Specifically, the OME used as an active agent in the present invention means that an aerobic microorganism and a specific basidiomycete are cultured in a bioactive agent previously applied for by the present inventors as a specific culture medium, Next, a different microorganism obtained by adding a plant-derived carbon source to a mixture of a microorganism group obtained by culturing an anaerobic microorganism in this culture solution and an enzyme that is a metabolite thereof to produce a carbon-degrading enzyme. It refers to a solution containing at least green bacteria as a photosynthetic bacterium based on a solution in which a group coexists and contains an enzyme as a metabolite and a carbon-degrading enzyme. Such activators are PCT / J
In the method for producing OME described in the specification of P99 / 12346, when culturing photosynthetic bacteria, it is obtained by introducing and culturing green bacteria. Also, OME
Green bacteria may be added to the mixture.

The activator of the present invention thus obtained is usually 300 to 3000 times, preferably 500 to 20 times.
Dilute with water 00 times before use. The OME on which this active agent is based has been shown to be harmless in oral toxicity tests in mice, as disclosed in the international patent application, and is applicable to various aqueous organisms of interest.
It also has the following features regarding optimization of the hydrosphere.

It has a carbonaceous dissolving action. It can be assumed that the OME active ingredient contains a carbon-degrading enzyme. The following microbial / enzymatic reactions occur selectively depending on the substance to be treated. Hydrolysis reaction a. RCO-NHR ′ + H ₂ O → RCOOH + R′NH ₂ b. RCO-OR ′ + H ₂ O → RCOOH + 4R′OH c. RCO-SR ′ + H ₂ O → RCOOH + 4R′SH d. R—CH—OR ′ + H ₂ O → RH + HO—CH—OR ′ (wherein, R and R ′ each independently represent a hydrocarbon group which may be substituted.) II Cleavage reaction a. RCOOH → RH + CO ₂ b. _{HOCRH-CR'H-OH → RCH 2} OH + R'CHO ( where, R, R 'each represent independently optionally substituted hydrocarbon group.) III. Redox reaction a. AH ₂ + B → A + BH ₂ b. AH ₂ + O ₂ → A + H ₂ O ₂ IV dehydrogenation reaction a. CRR'H-CR "H-OH → RR'C = CR" H + H 2 O b. _{CRR'H-CR "H-NH 2} → RR'C = CR" H + NH 2 ( wherein, R, R ', R "each represent independently optionally substituted hydrocarbon group.) V. dehydration Halogenation reaction a. RCX-CR'H → RC = CR '+ HX (wherein R represents an optionally substituted hydrocarbon group and X represents a halogen atom) VI. Substitution reaction a. RXCH ₂ + H ₂ O → RCH ₂ OH + HX b. RXCH ₂ + HS ⁻ → RCH ₂ SH + X ⁻ (wherein R represents a hydrocarbon group which may be substituted, and X represents a halogen atom.) Phenolic OH And halogen elimination reactions

Heavy Metal Removal Action OME has an action of removing heavy metals such as zinc, lead, tin, nickel, chromium, copper, cobalt, manganese, mercury, cadmium, and dross components in semiconductors. Decomposition action of organic compound By the above dehalogenation action, an organic halogen compound, for example, a halogen-substituted aromatic organic compound such as dioxins, polychlorinated biphenyls, and chlorobenzene, tetrachloroethylene, trichloroethylene, dichloromethane, carbon tetrachloride, 1,2 -Dichloroethylene, 1,1-
Dichloroethylene, cis-1,2-dichloroethylene,
It is capable of decomposing aliphatic organic halogen compounds such as 1,1,1-trichloroethane, 1,1,2-trichloroethane, 1,3-dichloropropene, decomposing pigments such as azo dyes, methyl mercaptan, and captans. , Idol,
It has the action of decomposing organic compounds such as skatole.

Decomposition of inorganic compounds Reduction of nitrogen: Anaerobic and facultative anaerobic chemoheterotrophs contained in OME have a mechanism of either anaerobic respiration or fermentation. Anaerobic respiration is essentially the same biochemical pathway as aerobic metabolism (aerobic respiration), where the final electron acceptor of the electron transport chain is nitrate (NO ₃ ⁻ ), sulfuric acid (SO 2) instead of oxygen. ₄ ^2-), fumaric acid or trimethylamine oxide. In the case of NO ₃ ⁻ and SO ₄ ²⁻ , it functions as a final electron acceptor of a reduction product. During the reduction, the denitrifying bacteria, NO ₃ ^{- -} NO ₃ is NO ₂ ^-, and the further reduced _{N 2} O, and the ultimately produce _{N 2} gas, the denitrification in the OM and OME A typical bacterium having is Rhodob.
acter, Thiobacillus, Cytoph
aga and the like.

Decomposition of ammonia Ammonia is made odorless in OM or OME by the following reaction. _{2NH 3 + H 2 O + CO} 2 → (NH 4) 2 CO 3 2 (NH 4 OH) + H 2 O + CO 2 → (NH 4) 2 C
O ₃ + 2H ₂ O ammonia water also presents ammonium carbonate, and when the supply of oxygen is small, ammonium ions (NH ₄ ⁻ )
Is changed from nitrous acid to nitric acid by digestive bacteria and the like, and becomes odorless. 2NH ₃ +20 ₂ → 2NHO + H ₂ O 2NHO ₂ + O ₂ → 2NHO ₃

Decomposition of hydrogen sulfide (H ₂ S) 2H ₂ S + O ₂ → 2NHO ₂ + H ₂ O Decomposition of methyl mercaptan (CH ₃ SH) 2CH ₃ SH + O ₂ → 2CH ₃ OH + S 2CH ₃ OH + 2O ₂ → 2CO ₂ + H ₂ O Seston When OME is put into a hydrosphere containing sestone, it has an effect of decomposing sestone. Water-bloom removal action When OME is applied to water-bloom generated on the water surface due to eutrophication of the hydrosphere, it has an action of instantly decomposing and removing it. OME is harmless.

In addition to these OME features, the activators according to the invention, which comprise at least green bacteria as photosynthetic fungi, are, more surprisingly, introduced into the aquatic zone where such aquatic organisms are raised, and It has been found that it has an action of decomposing and removing algae adhering to glass, concrete and the like of a fish cage.

Furthermore, when the activator according to the present invention is dripped into a water sphere into which a depleted or weakened aqueous animal, for example, a fish or a crustacean exhausted while being transported from abroad for a long time, is activated instantaneously. Found to be. Specifically, it is about 1 in the state of airlift from the Philippines
When the activator of the present invention obtained by diluting OME containing green bacteria 1000-fold to the shrimp shrimp that kills more than half a day was added, the inhabitants for more than 2 weeks were confirmed without changing the environment. This is expected that the interaction of green bacteria and cyanobacteria contained in the active agent according to the present invention will convert the hydrosphere into vitamin K or an oxygen releasing hydrosphere. It is essential that the activator according to the present invention contains at least green bacteria among photosynthetic bacteria, and it is preferable that substantially all of the photosynthetic bacteria are green bacteria.

Also, surprisingly, small fish can be stored in a closed system, which is usually difficult to breed, for example, in a closed system containing about 80% of water in a sphere having a diameter of several centimeters. It was possible to breed across. In particular,
A plastic container having a diameter of about 2 to 3 cm is filled with water in which 1 liter of OME is added with 0.1 cc of a 1000-fold diluted solution per liter of water, and a red fin, a fish having a total length of about 1 to 1.5 cm, can be bred. It has become possible. Further, when about 1 cc of the active agent of the present invention obtained by diluting OME containing green bacteria 1000 times with respect to about 2 liters of seawater was added, it was possible to breed a seahorse which was difficult to breed conventionally. It has become possible.

If desired, PCT / J by the inventor
DCP described in P99 / 12346
Alternatively, the effects of the present invention can be further improved by dispersing RCS or the like in the hydrosphere.

[0021]

As described above, the present invention has the following excellent effects. 1. When the activator of the present invention is used, exhausted aquatic organisms can be activated irrespective of a closed environment or an open environment, and at the same time, blue water, algae, excrement, seston, etc. existing in the aquatic sphere of the aquatic organism. It is possible to purify chemical hazards and the like released from industry and the like. 2. In addition, in this way, it is possible to effectively breed aqueous animals in the hydrosphere containing the active agent according to the present invention. In particular, even in a closed system, small fish and the like can be bred for a long period of time.

Claims (9)

[Claims] 1. An activator based on a microorganism and a metabolite thereof for activating an aquatic organism and optimizing a hydrosphere for breeding the aquatic organism, comprising: (a) a group of aerobic microorganisms;
Anaerobic microorganisms, (c) Basidiomycetes belonging to at least one kind of Pleurotus, (d) Photosynthetic fungi containing at least green bacteria as essential components, and enzymes and carbon decomposition derived from metabolites of these microorganisms An activator, comprising a microbial culture solution containing an enzyme. 2. A method for optimizing the aquatic zone for breeding said aquatic organisms simultaneously with activating the aquatic organisms, wherein the activator according to claim 1 is used as a living area for the aquatic organisms. A method for purifying a hydrosphere and activating aquatic organisms. 3. The method of claim 2, wherein said hydrosphere is a hydrosphere contaminated by eutrophication. 4. The method of claim 2, wherein said hydrosphere is a closed hydrosphere. 5. The method according to claim 1, wherein the activator is used in an amount of 0.1 to 1 liter of the hydrosphere.
3. The method according to claim 2, wherein the addition amount is from 0.1 to 1.0 cc.
5. The method according to any one of items 4 to 4. 6. A method for activating a weakened aqueous organism, the method comprising activating the active agent according to claim 1 to a target aqueous organism. 7. A method for breeding aquatic organisms, which comprises introducing the active agent according to claim 1 into the hydrosphere of the marine organism to be bred. 8. The method according to claim 6, wherein the aqueous organism is bred in a closed container. 9. An accessory comprising an airtight container, water containing the active agent of claim 1 enclosed in said airtight container, and at least one aqueous organism.