JP2000236874A5 - - Google Patents
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- Publication number
- JP2000236874A5 JP2000236874A5 JP1999314907A JP31490799A JP2000236874A5 JP 2000236874 A5 JP2000236874 A5 JP 2000236874A5 JP 1999314907 A JP1999314907 A JP 1999314907A JP 31490799 A JP31490799 A JP 31490799A JP 2000236874 A5 JP2000236874 A5 JP 2000236874A5
- Authority
- JP
- Japan
- Prior art keywords
- hydrazine
- cysteine
- hydroxylamine
- analogs
- compound selected
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 description 8
- 150000001875 compounds Chemical class 0.000 description 6
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 description 4
- 102000004317 Lyases Human genes 0.000 description 3
- 108090000856 Lyases Proteins 0.000 description 3
- 239000004202 carbamide Substances 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- PWKSKIMOESPYIA-UHFFFAOYSA-N 2-acetamido-3-sulfanylpropanoic acid Chemical compound CC(=O)NC(CS)C(O)=O PWKSKIMOESPYIA-UHFFFAOYSA-N 0.000 description 2
- -1 L-cysteine, L-cysteine analogs Chemical class 0.000 description 2
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 2
- 150000002429 hydrazines Chemical class 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- DUIOPKIIICUYRZ-UHFFFAOYSA-N semicarbazide Chemical compound NNC(N)=O DUIOPKIIICUYRZ-UHFFFAOYSA-N 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
Description
【特許請求の範囲】
【請求項１】
Ｌ−システイン、Ｌ−システイン類縁体、ヒドラジン、ヒドラジン類縁体及びヒドロキシルアミンから選ばれる少なくとも１種の化合物と、ウレアアミドリアーゼとを共存させる、ウレアアミドリアーゼの安定化方法。
【請求項２】
前記化合物が、Ｌ−システイン、ヒドラジン、セミカルバジド及びヒドロキシルアミンから選ばれる少なくとも１種の化合物である請求項１に記載の安定化方法。
【請求項３】
Ｌ−システイン、Ｌ−システイン類縁体、ヒドラジン、ヒドラジン類縁体及びヒドロキシルアミンから選ばれる少なくとも１種の化合物と、ウレアアミドリアーゼを含有する尿素窒素測定試薬。
【請求項４】
前記化合物が、Ｌ−システイン、ヒドラジン、セミカルバジド及びヒドロキシルアミンから選ばれる少なくとも１種の化合物である請求項３に記載の安尿素窒素測定試薬。
【請求項５】
酸化防止剤を含有する請求項４に記載の尿素窒素測定試薬。
[Claims]
(1)
A method for stabilizing urea amide lyase, comprising allowing at least one compound selected from L-cysteine, L-cysteine analogs, hydrazine, hydrazine analogs and hydroxylamine to coexist with urea amide lyase.
(2)
The method according to claim 1, wherein the compound is at least one compound selected from L-cysteine, hydrazine, semicarbazide, and hydroxylamine.
(3)
A urea nitrogen measurement reagent containing at least one compound selected from L-cysteine, L-cysteine analogs, hydrazine, hydrazine analogs and hydroxylamine, and urea amide lyase.
(4)
The reagent according to claim 3, wherein the compound is at least one compound selected from L-cysteine, hydrazine, semicarbazide, and hydroxylamine.
Claim 5.
The reagent for measuring urea nitrogen according to claim 4, further comprising an antioxidant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31490799A JP4413340B2 (en) | 1998-12-25 | 1999-11-05 | Enzyme stabilization method and stabilized measurement reagent |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10-370649 | 1998-12-25 | ||
| JP37064998 | 1998-12-25 | ||
| JP31490799A JP4413340B2 (en) | 1998-12-25 | 1999-11-05 | Enzyme stabilization method and stabilized measurement reagent |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2000236874A JP2000236874A (en) | 2000-09-05 |
| JP2000236874A5 true JP2000236874A5 (en) | 2006-12-14 |
| JP4413340B2 JP4413340B2 (en) | 2010-02-10 |
Family
ID=26568108
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP31490799A Expired - Fee Related JP4413340B2 (en) | 1998-12-25 | 1999-11-05 | Enzyme stabilization method and stabilized measurement reagent |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4413340B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2011024513A (en) * | 2009-07-28 | 2011-02-10 | Nitto Boseki Co Ltd | Method for measuring dehydrogenase or the corresponding substrate by dry chemistry, and inspection instrument to be used therefor |
| JP2011103826A (en) * | 2009-11-19 | 2011-06-02 | Nitto Boseki Co Ltd | Method for measuring adp or specific substance producing adp by enzymatic reaction by dry chemistry and examination instrument used therefor |
| JP5843072B2 (en) * | 2010-04-30 | 2016-01-13 | 日東紡績株式会社 | Method for measuring specific substance and kit for measuring specific substance |
| CN110702676A (en) * | 2019-11-14 | 2020-01-17 | 北京华宇亿康生物工程技术有限公司 | Kit and method for detecting 1,5-AG with good stability |
-
1999
- 1999-11-05 JP JP31490799A patent/JP4413340B2/en not_active Expired - Fee Related
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