IN2014DE00669AIN669DE2014AIN2014DE00669AIN 2014DE00669 AIN2014DE00669 AIN 2014DE00669AIN 669DE2014 AIN669DE2014 AIN 669DE2014AIN 2014DE00669 AIN2014DE00669 AIN 2014DE00669A
Authority
IN
India
Prior art keywords
malz
aconitase
chaperone
coli
proteins simultaneously
Prior art date
Application number
Inventor
K Chaudhuri Tapan
Goyal Megha
Original Assignee
Indian Inst Technology Delhi
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Publication date
Application filed by Indian Inst Technology DelhifiledCriticalIndian Inst Technology Delhi
Priority to IN669DE2014priorityCriticalpatent/IN2014DE00669A/en
Publication of IN2014DE00669ApublicationCriticalpatent/IN2014DE00669A/en
102000004169proteins and genesHuman genes0.000abstract1
108090000623proteins and genesProteins0.000abstract1
Landscapes
Enzymes And Modification Thereof
(AREA)
Peptides Or Proteins
(AREA)
Abstract
A process has been provided for chaperone assisted folding of multiple aggregation prone recombinant proteins simultaneously in E.coli comprising of preparation of an expression system for co-expressing MalZ and aconitase in presence of GroEL-GroES in E.coli, subjecting MalZ and aconitase to the step of chaperone assisted folding to obtain functional form of both the proteins simultaneously.