IN2012DN03823A - - Google Patents
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- Publication number
- IN2012DN03823A IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
- Authority
- IN
- India
- Prior art keywords
- modified
- vacuolar sorting
- vps10
- sorting activity
- host cell
- Prior art date
Links
- 230000000694 effects Effects 0.000 abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 210000005253 yeast cell Anatomy 0.000 abstract 2
- 241000235058 Komagataella pastoris Species 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 230000003247 decreasing effect Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25637909P | 2009-10-30 | 2009-10-30 | |
| US35066810P | 2010-06-02 | 2010-06-02 | |
| PCT/US2010/053903 WO2011053541A1 (fr) | 2009-10-30 | 2010-10-25 | Procédés de production de protéines recombinantes présentant de meilleurs rendements de sécrétions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2012DN03823A true IN2012DN03823A (fr) | 2015-08-28 |
Family
ID=43922476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN3823DEN2012 IN2012DN03823A (fr) | 2009-10-30 | 2010-10-25 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20130011875A1 (fr) |
| EP (1) | EP2494053A4 (fr) |
| JP (1) | JP2013509180A (fr) |
| KR (1) | KR20140015137A (fr) |
| CN (1) | CN102686731A (fr) |
| AU (1) | AU2010313608A1 (fr) |
| BR (1) | BR112012009886A2 (fr) |
| CA (1) | CA2777487A1 (fr) |
| IN (1) | IN2012DN03823A (fr) |
| MX (1) | MX2012004993A (fr) |
| RU (1) | RU2012122166A (fr) |
| WO (1) | WO2011053541A1 (fr) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120213728A1 (en) * | 2009-10-30 | 2012-08-23 | Merck | Granulocyte-colony stimulating factor produced in glycoengineered pichia pastoris |
| US9102969B2 (en) * | 2011-03-11 | 2015-08-11 | Kaneka Corporation | Method for producing heterologous protein using yeast with disruption of VPS gene |
| US9567596B2 (en) | 2012-01-05 | 2017-02-14 | Glykos Finland Oy | Protease deficient filamentous fungal cells and methods of use thereof |
| CN102559740B (zh) * | 2012-02-25 | 2013-03-27 | 山东大学 | 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株 |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| WO2014136113A1 (fr) | 2013-03-06 | 2014-09-12 | Protalix Ltd. | Polypeptides chimériques, polynucléotides codant pour ceux-ci, cellules exprimant ceux-ci et leurs procédés de production |
| MX2016000306A (es) | 2013-07-10 | 2016-08-08 | Novartis Ag | Celulas de hongos filamentosos deficientes en multiples proteasas y métodos de uso de las mismas. |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| BR112017010555A2 (pt) * | 2014-11-20 | 2018-02-27 | Yissum Res Dev Co Of Hebrew Univ Jerusalem Ltd | composições e métodos para produção de polipeptídeos com padrão de glicosilação modificado em células vegetais |
| ES2902044T3 (es) * | 2015-08-05 | 2022-03-24 | Lonza Ag | Variantes de promotor |
| WO2018132821A2 (fr) | 2017-01-13 | 2018-07-19 | Bolt Threads, Inc. | Protéines élastomères |
| WO2019027364A1 (fr) | 2017-07-31 | 2019-02-07 | Biopetrolia Ab | Cellule fongique à capacité de production de protéine améliorée |
| SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
| CN113604373A (zh) * | 2021-02-08 | 2021-11-05 | 江南大学 | 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株 |
| CN113736818A (zh) * | 2021-10-08 | 2021-12-03 | 江南大学 | 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法 |
| CN113862242B (zh) * | 2021-10-15 | 2024-06-25 | 江南大学 | 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法 |
| EP4194560A1 (fr) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Production améliorée de protéines sécrétées dans des cellules fongiques |
| CN115851468A (zh) * | 2022-07-18 | 2023-03-28 | 江南大学 | 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用 |
| CN116478905B (zh) * | 2023-02-15 | 2025-07-22 | 北京中医药大学 | 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用 |
| WO2025000334A1 (fr) * | 2023-06-29 | 2025-01-02 | 中国科学院深圳先进技术研究院 | Protéine de fusion et son utilisation dans la préparation de métabolites |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| JP4969011B2 (ja) | 2000-05-17 | 2012-07-04 | 田辺三菱製薬株式会社 | 酸性糖鎖減少蛋白質の製造方法および該製造された糖蛋白質 |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| ATE309385T1 (de) | 2000-06-28 | 2005-11-15 | Glycofi Inc | Verfahren für die herstellung modifizierter glykoproteine |
| GB0121088D0 (en) * | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
| MXPA04006357A (es) | 2001-12-27 | 2005-03-31 | Glycofi Inc | Metodos para disenar estructuras de carbohidrato del tipo de mamifero. |
| US7252933B2 (en) * | 2002-06-26 | 2007-08-07 | Flanders Interuniversity Institute For Biotechnology | Protein glycosylation modification in methylotrophic yeast |
| CA2505573A1 (fr) | 2002-11-12 | 2004-05-27 | Purdue Research Foundation | Promoteurs inductibles par du benzoate |
| AU2004311545B2 (en) | 2003-12-24 | 2008-11-06 | Glycofi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
| US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
| AU2005224672B2 (en) | 2004-03-17 | 2011-06-02 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| JP4954866B2 (ja) | 2004-04-29 | 2012-06-20 | グライコフィ， インコーポレイテッド | 糖タンパク質の作製においてアルファ−マンノシダーゼ抵抗性グリカンを減少させるか又は排除する方法 |
| US20060252069A1 (en) | 2005-04-21 | 2006-11-09 | Zhang Kunyan | Pcr for mrsa sccmec typing |
| CN103255162A (zh) * | 2006-05-19 | 2013-08-21 | 格利科菲公司 | 促红细胞生成素组合物 |
| US8741633B2 (en) | 2006-05-19 | 2014-06-03 | Glycofi, Inc. | Recombinant vectors |
-
2010
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/fr not_active Withdrawn
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/fr not_active Ceased
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/pt not_active IP Right Cessation
- 2010-10-25 CA CA2777487A patent/CA2777487A1/fr not_active Abandoned
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/ja not_active Withdrawn
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/ko not_active Withdrawn
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/ru not_active Application Discontinuation
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/zh active Pending
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/es not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| EP2494053A1 (fr) | 2012-09-05 |
| US20130011875A1 (en) | 2013-01-10 |
| EP2494053A4 (fr) | 2013-10-30 |
| KR20140015137A (ko) | 2014-02-06 |
| JP2013509180A (ja) | 2013-03-14 |
| RU2012122166A (ru) | 2013-12-10 |
| BR112012009886A2 (pt) | 2015-09-15 |
| CN102686731A (zh) | 2012-09-19 |
| AU2010313608A1 (en) | 2012-06-07 |
| CA2777487A1 (fr) | 2011-05-05 |
| WO2011053541A1 (fr) | 2011-05-05 |
| MX2012004993A (es) | 2012-06-12 |
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