IL179559A - Thermotine modulators - Google Patents
Thermotine modulatorsInfo
- Publication number
- IL179559A IL179559A IL179559A IL17955906A IL179559A IL 179559 A IL179559 A IL 179559A IL 179559 A IL179559 A IL 179559A IL 17955906 A IL17955906 A IL 17955906A IL 179559 A IL179559 A IL 179559A
- Authority
- IL
- Israel
- Prior art keywords
- theramutein
- phenoresponse
- prototheramutein
- cell
- substance
- Prior art date
Links
- 238000000034 method Methods 0.000 claims 13
- 210000004027 cell Anatomy 0.000 claims 12
- 239000000126 substance Substances 0.000 claims 8
- 239000012190 activator Substances 0.000 claims 7
- 239000003112 inhibitor Substances 0.000 claims 7
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 102000001253 Protein Kinase Human genes 0.000 claims 1
- 102000004022 Protein-Tyrosine Kinases Human genes 0.000 claims 1
- 108090000412 Protein-Tyrosine Kinases Proteins 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 230000000877 morphologic effect Effects 0.000 claims 1
- 230000026731 phosphorylation Effects 0.000 claims 1
- 238000006366 phosphorylation reaction Methods 0.000 claims 1
- 108060006633 protein kinase Proteins 0.000 claims 1
- 102000027426 receptor tyrosine kinases Human genes 0.000 claims 1
- 108091008598 receptor tyrosine kinases Proteins 0.000 claims 1
- 230000019491 signal transduction Effects 0.000 claims 1
- 210000001768 subcellular fraction Anatomy 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
- 230000001052 transient effect Effects 0.000 claims 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Claims (13)
1. A method. for determining whether a substance is an inhibitor or an activator of a theramutein which is capable of eliciting a detectable phenoresponse, which comprises: a) incubating a first cell which expresses the theramutein at a substantially constant level with the substance; b) incubating a second cell which expresses a corresponding prototheramutein at a substantially constant level with a known inhibitor or activator of the prototheramutein; c) comparing a phenoresponse of the second cell to the known inhibitor or activator of the protothera utein to the phenoresponse of the first cell to the substance; and d) determining that the phenoresponse of the first cell is inhibited or activated to at least the same degree as the phenoresponse of the second cel l is inhibited or activated by the known inhibitor or activator of the prototheramutein, thereby identifying the substance as an inhibitor or an activator of the theramutein.
2. The method of Claim 1 , wherein the phenoresponse of the cell expressing the theramutein to the substance is greater than the phenoresponse of the cell expressing the prototheramutein to the known inhibitor or activator of the prototheramutein.
3. A method for determining whether a substance is a specific inhibitor or specific activator of a theramutein, which comprises: a) providing a test cell which expresses the theramutein and which gives rise to a detectable phenoresponse; b) treating the test cell with the substance; c) examining the treated cell to determine whether the phenoresponse is modulated by treatment with the substance.
4. The method of Claim 1 or 3, wherein the theramutein or prototheramutein is a component of a signal transduction cascade.
5. The method of Claim 1 or 3, wherein the theramutein or prototheramutein is an enzyme. - 78 - 1 79559/4
6. The method of Claim 1 or 3, wherein the theramutein or prototheramutein is a protein kinase.
7. The method of Claim 1 or 3, wherein the theramutein or prototheramutein is a tyrosine kinase.
8. The method of Claim 1 or 3, wherein the theramutein or prototheramutein is a receptor tyrosine kinase.
9. The method of Claim 1 or 3, wherein the prototheramutein is p210Bcr"Abl.
10. The method of Claim 1 or 3, wherein the theramutein is the T3 1 51 mutant of p210Bcr-Abl.
11. 1 1. The method of Claim 1 or 3, wherein the phenoresponse is a change in a cultural, morphological, or transient characteristic of the cell.
12. 1 2. The method of Claim 1 or 3, wherein the phenoresponse includes phosphorylation of an intracellular substrate of the theramutein.
13. The method of Claim 1 or 3, wherein the phenoresponse is detected on a subcellular fraction of the cell. P-9670-1L - 79 -
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57396204P | 2004-05-23 | 2004-05-23 | |
| US63053404P | 2004-11-22 | 2004-11-22 | |
| US63301304P | 2004-12-03 | 2004-12-03 | |
| PCT/US2005/018412 WO2005115992A1 (en) | 2004-05-23 | 2005-05-23 | Theramutein modulators |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL179559A0 IL179559A0 (en) | 2007-05-15 |
| IL179559A true IL179559A (en) | 2014-09-30 |
Family
ID=40290709
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL179559A IL179559A (en) | 2004-05-23 | 2006-11-23 | Thermotine modulators |
Country Status (2)
| Country | Link |
|---|---|
| IL (1) | IL179559A (en) |
| MX (1) | MXPA06013648A (en) |
-
2005
- 2005-05-23 MX MXPA06013648A patent/MXPA06013648A/en active IP Right Grant
-
2006
- 2006-11-23 IL IL179559A patent/IL179559A/en active IP Right Grant
Also Published As
| Publication number | Publication date |
|---|---|
| IL179559A0 (en) | 2007-05-15 |
| MXPA06013648A (en) | 2007-10-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |