IL126192A - Method for detection of contaminants - Google Patents
Method for detection of contaminantsInfo
- Publication number
- IL126192A IL126192A IL12619297A IL12619297A IL126192A IL 126192 A IL126192 A IL 126192A IL 12619297 A IL12619297 A IL 12619297A IL 12619297 A IL12619297 A IL 12619297A IL 126192 A IL126192 A IL 126192A
- Authority
- IL
- Israel
- Prior art keywords
- membrane
- test device
- disposable test
- disposable
- fluid
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 115
- 238000001514 detection method Methods 0.000 title claims description 23
- 239000000356 contaminant Substances 0.000 title description 12
- 239000012528 membrane Substances 0.000 claims abstract description 126
- 238000012360 testing method Methods 0.000 claims abstract description 126
- 239000012530 fluid Substances 0.000 claims abstract description 78
- 230000001580 bacterial effect Effects 0.000 claims abstract description 43
- 239000005089 Luciferase Substances 0.000 claims abstract description 26
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 24
- 239000000126 substance Substances 0.000 claims abstract description 16
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 13
- 108060001084 Luciferase Proteins 0.000 claims abstract description 12
- IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 claims abstract description 9
- CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 claims abstract description 9
- BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 claims abstract description 9
- DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000012141 concentrate Substances 0.000 claims abstract description 6
- 244000005700 microbiome Species 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 50
- ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 claims description 44
- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 44
- 239000002250 absorbent Substances 0.000 claims description 32
- 230000002745 absorbent Effects 0.000 claims description 32
- 238000006243 chemical reaction Methods 0.000 claims description 28
- 239000012491 analyte Substances 0.000 claims description 26
- 239000003795 chemical substances by application Substances 0.000 claims description 21
- 239000011521 glass Substances 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 18
- 210000004027 cell Anatomy 0.000 claims description 17
- 239000000427 antigen Substances 0.000 claims description 14
- 102000036639 antigens Human genes 0.000 claims description 14
- 108091007433 antigens Proteins 0.000 claims description 14
- 239000003599 detergent Substances 0.000 claims description 13
- 239000004033 plastic Substances 0.000 claims description 13
- 239000000123 paper Substances 0.000 claims description 12
- 239000002245 particle Substances 0.000 claims description 11
- 239000000758 substrate Substances 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 11
- 150000003839 salts Chemical class 0.000 claims description 9
- 108090000790 Enzymes Proteins 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 8
- 239000011230 binding agent Substances 0.000 claims description 8
- 241000700605 Viruses Species 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 6
- 239000011111 cardboard Substances 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 150000002500 ions Chemical class 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 2
- HUDPLKWXRLNSPC-UHFFFAOYSA-N 4-aminophthalhydrazide Chemical compound O=C1NNC(=O)C=2C1=CC(N)=CC=2 HUDPLKWXRLNSPC-UHFFFAOYSA-N 0.000 claims description 2
- 229920000742 Cotton Polymers 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 claims description 2
- 239000007995 HEPES buffer Substances 0.000 claims description 2
- 102000004856 Lectins Human genes 0.000 claims description 2
- 108090001090 Lectins Proteins 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- 108090000854 Oxidoreductases Proteins 0.000 claims description 2
- 102000004316 Oxidoreductases Human genes 0.000 claims description 2
- 102000003992 Peroxidases Human genes 0.000 claims description 2
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 2
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims description 2
- 230000001420 bacteriolytic effect Effects 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 239000011152 fibreglass Substances 0.000 claims description 2
- 239000002523 lectin Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 2
- 238000005286 illumination Methods 0.000 claims 4
- 230000000717 retained effect Effects 0.000 claims 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000007244 Zea mays Nutrition 0.000 claims 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims 1
- 229940089639 cornsilk Drugs 0.000 claims 1
- 239000012266 salt solution Substances 0.000 claims 1
- 239000001231 zea mays silk Substances 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 abstract description 23
- 238000011109 contamination Methods 0.000 abstract description 15
- 239000007788 liquid Substances 0.000 abstract description 13
- 238000001914 filtration Methods 0.000 abstract description 9
- 238000004020 luminiscence type Methods 0.000 abstract description 3
- 230000000392 somatic effect Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 41
- 241000894006 Bacteria Species 0.000 description 28
- 239000012071 phase Substances 0.000 description 21
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 235000013305 food Nutrition 0.000 description 10
- 238000003556 assay Methods 0.000 description 9
- 238000012545 processing Methods 0.000 description 8
- 239000000725 suspension Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 241000607142 Salmonella Species 0.000 description 6
- 230000002934 lysing effect Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 239000010802 sludge Substances 0.000 description 6
- 229920001213 Polysorbate 20 Polymers 0.000 description 5
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 5
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000006150 trypticase soy agar Substances 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000005415 bioluminescence Methods 0.000 description 3
- 230000029918 bioluminescence Effects 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 230000002452 interceptive effect Effects 0.000 description 3
- 239000004816 latex Substances 0.000 description 3
- 229920000126 latex Polymers 0.000 description 3
- 239000006194 liquid suspension Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 239000012465 retentate Substances 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 230000014670 detection of bacterium Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 230000016507 interphase Effects 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 235000020030 perry Nutrition 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000008399 tap water Substances 0.000 description 2
- 235000020679 tap water Nutrition 0.000 description 2
- 210000005253 yeast cell Anatomy 0.000 description 2
- 241000607534 Aeromonas Species 0.000 description 1
- 241000607528 Aeromonas hydrophila Species 0.000 description 1
- 108090000363 Bacterial Luciferases Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 206010016952 Food poisoning Diseases 0.000 description 1
- 208000019331 Foodborne disease Diseases 0.000 description 1
- 241001245789 Goodea atripinnis Species 0.000 description 1
- 241000254158 Lampyridae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000293871 Salmonella enterica subsp. enterica serovar Typhi Species 0.000 description 1
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 241000193996 Streptococcus pyogenes Species 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000013000 chemical inhibitor Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000001332 colony forming effect Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 229920001971 elastomer Polymers 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- LIYGYAHYXQDGEP-UHFFFAOYSA-N firefly oxyluciferin Natural products Oc1csc(n1)-c1nc2ccc(O)cc2s1 LIYGYAHYXQDGEP-UHFFFAOYSA-N 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012569 microbial contaminant Substances 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 239000002991 molded plastic Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- JJVOROULKOMTKG-UHFFFAOYSA-N oxidized Photinus luciferin Chemical compound S1C2=CC(O)=CC=C2N=C1C1=NC(=O)CS1 JJVOROULKOMTKG-UHFFFAOYSA-N 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 230000001175 peptic effect Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 238000003908 quality control method Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- NXLOLUFNDSBYTP-UHFFFAOYSA-N retene Chemical compound C1=CC=C2C3=CC=C(C(C)C)C=C3C=CC2=C1C NXLOLUFNDSBYTP-UHFFFAOYSA-N 0.000 description 1
- 229930187593 rose bengal Natural products 0.000 description 1
- AZJPTIGZZTZIDR-UHFFFAOYSA-L rose bengal Chemical compound [K+].[K+].[O-]C(=O)C1=C(Cl)C(Cl)=C(Cl)C(Cl)=C1C1=C2C=C(I)C(=O)C(I)=C2OC2=C(I)C([O-])=C(I)C=C21 AZJPTIGZZTZIDR-UHFFFAOYSA-L 0.000 description 1
- 229940081623 rose bengal Drugs 0.000 description 1
- STRXNPAVPKGJQR-UHFFFAOYSA-N rose bengal A Natural products O1C(=O)C(C(=CC=C2Cl)Cl)=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 STRXNPAVPKGJQR-UHFFFAOYSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2304/00—Chemical means of detecting microorganisms
- C12Q2304/60—Chemiluminescent detection using ATP-luciferin-luciferase system
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N2001/028—Sampling from a surface, swabbing, vaporising
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- General Engineering & Computer Science (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/785,833 US5736351A (en) | 1995-01-09 | 1997-01-15 | Method for detection of contaminants |
| PCT/US1997/004289 WO1998032020A1 (fr) | 1997-01-15 | 1997-03-25 | Procede de detection de contaminants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL126192A0 IL126192A0 (en) | 1999-05-09 |
| IL126192A true IL126192A (en) | 2001-05-20 |
Family
ID=25136757
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL12619297A IL126192A (en) | 1997-01-15 | 1997-03-25 | Method for detection of contaminants |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US5736351A (fr) |
| EP (2) | EP1460427A1 (fr) |
| JP (1) | JP2002514916A (fr) |
| CN (1) | CN1154847C (fr) |
| AT (1) | ATE274700T1 (fr) |
| AU (1) | AU2533297A (fr) |
| CA (1) | CA2249370A1 (fr) |
| DE (1) | DE69730425T2 (fr) |
| IL (1) | IL126192A (fr) |
| PL (1) | PL189724B1 (fr) |
| WO (1) | WO1998032020A1 (fr) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| AT409190B (de) * | 1999-01-18 | 2002-06-25 | Helmut Dr Pfuetzner | Kontaminationswächter |
| US6174699B1 (en) * | 1999-03-09 | 2001-01-16 | 3M Innovative Properties Company | Disc assay device with inoculation pad and methods of use |
| DE19928887A1 (de) * | 1999-06-24 | 2000-12-28 | Tetra Laval Holdings & Finance | Verfahren und Vorrichtung zur Erfassung der Keimbelastung |
| AU3457101A (en) * | 2000-01-26 | 2001-08-07 | Univ Loma Linda | Method for the evaluation of implantable materials |
| US6548018B2 (en) * | 2000-03-31 | 2003-04-15 | Neogen Corporation | Apparatus for chemiluminescent assays |
| US7060223B2 (en) * | 2000-03-31 | 2006-06-13 | Neogen Corporation | Polymeric medium for the retention of reagent species for use in a hand-held device for the relatively rapid detection of the presence of an analyte of interest in a sample |
| JP2003102496A (ja) * | 2001-09-27 | 2003-04-08 | Matsushita Ecology Systems Co Ltd | 微生物検査システム |
| US6699684B2 (en) * | 2002-07-23 | 2004-03-02 | Nalco Company | Method of monitoring biofouling in membrane separation systems |
| AU2003279759A1 (en) * | 2002-10-03 | 2004-04-23 | Heska Corporation | Fluid collecting device, methods of production and uses thereof |
| JP4157400B2 (ja) * | 2003-03-13 | 2008-10-01 | 株式会社トクヤマ | 抗原抽出液の製造方法。 |
| CN1208113C (zh) * | 2003-05-01 | 2005-06-29 | 东南大学 | 用于空气中微量物质收集的装置及其收集方法 |
| US7132249B1 (en) | 2003-05-12 | 2006-11-07 | Charm Sciences, Inc. | Method of determining allergenic food on surfaces |
| US7494781B1 (en) | 2003-05-12 | 2009-02-24 | Charm Sciences, Inc. | Sensitive method for detecting low levels of ATP |
| JP5026074B2 (ja) * | 2003-08-20 | 2012-09-12 | メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング | 疎水性液体マトリクス中に拡散または分散した親水性化合物の抽出および濃縮の方法 |
| US20050070701A1 (en) * | 2003-09-29 | 2005-03-31 | Hochstetler Spencer Erich | Detection of living cells in polymers or pigments |
| US7419798B2 (en) | 2004-10-19 | 2008-09-02 | Zybac Llc | Rapid and sensitive detection of bacteria in blood products, urine, and other fluids |
| US20080305538A1 (en) * | 2004-10-19 | 2008-12-11 | Medical Innovations International, Inc. | Rapid and Sensitive Detection of Bacteria in Blood Products, Urine, and Other Fluids |
| US7993871B2 (en) | 2004-12-22 | 2011-08-09 | Charm Sciences, Inc. | Sampling method and device |
| FR2880035A1 (fr) * | 2004-12-23 | 2006-06-30 | Eads Ccr Groupement D Interet | Procede de detection de la biocontamination d'un combustible liquide |
| NL1030525C2 (nl) * | 2005-11-25 | 2007-05-29 | Amiris B V | Werkwijze voor het met luminescentie detecteren van ATP in een monster en een computerprogramma daarvoor. |
| CN1804602B (zh) * | 2005-12-15 | 2010-05-05 | 卢麟麟 | 表面清洁程度及细菌污染快速检测方法和装置 |
| US8476064B2 (en) | 2006-05-09 | 2013-07-02 | Charm Sciences, Inc. | Inhibition assay method and device for detection of antibiotics |
| CN101748185A (zh) * | 2008-12-02 | 2010-06-23 | 实创国际生技股份有限公司 | 胃幽门螺旋杆菌的检测试片及其检测方法 |
| CN101348827B (zh) * | 2008-07-29 | 2011-07-20 | 常俊山 | 洗气法检测空气浮游菌及其专用检测瓶 |
| JP5386752B2 (ja) * | 2009-04-21 | 2014-01-15 | 国立大学法人 東京大学 | 微生物の数または量を測定する方法および装置 |
| CN102191162B (zh) * | 2010-03-19 | 2013-08-21 | 南台湾环境科技股份有限公司 | 空气中微生物活性实时侦测装置及方法 |
| US20190300945A1 (en) | 2010-04-05 | 2019-10-03 | Prognosys Biosciences, Inc. | Spatially Encoded Biological Assays |
| RS54482B1 (sr) | 2010-04-05 | 2016-06-30 | Prognosys Biosciences, Inc. | Biološka testiranja sa prostornim kodiranjem |
| US10787701B2 (en) | 2010-04-05 | 2020-09-29 | Prognosys Biosciences, Inc. | Spatially encoded biological assays |
| US10514330B2 (en) * | 2010-07-14 | 2019-12-24 | Qiagen Gmbh | Device for isolation and/or purification of biomolecules |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| TWI406712B (zh) * | 2011-05-18 | 2013-09-01 | Taigen Bioscience Corp | 安全處理生化檢體之裝置 |
| CN103045714B (zh) * | 2011-10-12 | 2015-08-12 | 中国农业科学院棉花研究所 | 用于检测棉花纤维上携带的微生物的方法 |
| CN102367469B (zh) * | 2011-10-24 | 2013-07-31 | 宁波大学 | 一种夜光藻活细胞的定量浓缩方法 |
| EP3901280B1 (fr) | 2012-10-17 | 2025-03-12 | 10x Genomics Sweden AB | Procédés et produits pour optimiser la detection localisée ou spatiale de l'expression génique dans un échantillon de tissu |
| LT3013983T (lt) | 2013-06-25 | 2023-05-10 | Prognosys Biosciences, Inc. | Erdviniai koduoti biologiniai tyrimai, naudojant mikrofluidinį įrenginį |
| ITRM20130128U1 (it) * | 2013-07-23 | 2015-01-24 | Particle Measuring Systems S R L | Dispositivo per il campionamento microbico dell'aria |
| US10955314B1 (en) * | 2015-01-06 | 2021-03-23 | The United States Of America As Represented By The Secretary Of The Army | One-handed surface sampling device |
| JP6821586B2 (ja) * | 2015-03-19 | 2021-01-27 | スリーエム イノベイティブ プロパティズ カンパニー | 流体試料中の微生物株又は標的細胞検体を検出するためのデバイス、方法、キット、及びシステム |
| HK1250245A1 (zh) | 2015-04-10 | 2018-12-07 | Spatial Transcriptomics Ab | 生物样本的空间区别、多重核酸分析 |
| CN104931700B (zh) * | 2015-06-01 | 2017-03-08 | 上海凯创生物技术有限公司 | 沙门氏菌乳胶法检测试剂盒 |
| DE102016123658B4 (de) * | 2016-12-07 | 2021-10-07 | Technische Universität Dresden | Filtrationsvorrichtung und Verfahren zur Anreicherung von Targets und der nachfolgenden Freisetzung biogener Agenzien |
| CN106680474A (zh) * | 2017-02-04 | 2017-05-17 | 上海为然环保科技有限公司 | 一种家用餐具大肠杆菌快速检测仪 |
| GB201703383D0 (en) | 2017-03-02 | 2017-04-19 | Gargle Tech Ltd | Testing for particulates |
| CN107449633B (zh) * | 2017-08-18 | 2023-08-01 | 国家深海基地管理中心 | 一种热液区喷发物质捕集装置 |
| CN115430471A (zh) | 2018-09-05 | 2022-12-06 | 英雄科学有限公司 | 微粒测试的设备及方法 |
| WO2020102032A1 (fr) | 2018-11-16 | 2020-05-22 | Particle Measuring Systems, Inc. | Systèmes et procédés d'échantillonnage de particules destinés à des systèmes de barrière de fabrication commandés par robotique |
| EP4567127A3 (fr) | 2018-12-10 | 2025-09-03 | 10x Genomics, Inc. | Procédés de détermination d'un emplacement d'un analyte biologique dans un échantillon biologique |
| US12157124B2 (en) | 2019-11-06 | 2024-12-03 | 10X Genomics, Inc. | Imaging system hardware |
| TWI801797B (zh) | 2020-01-21 | 2023-05-11 | 美商粒子監測系統有限公司 | 撞擊器及用於對來自一流體流之生物顆粒取樣之方法 |
| US11768175B1 (en) | 2020-03-04 | 2023-09-26 | 10X Genomics, Inc. | Electrophoretic methods for spatial analysis |
| WO2021181339A1 (fr) | 2020-03-11 | 2021-09-16 | Hero Scientific Ltd. | Dispositifs de test |
| KR102333308B1 (ko) * | 2020-05-07 | 2021-12-01 | 전남대학교산학협력단 | 바이러스 또는 미세 입자 검출 장치 |
| US12031177B1 (en) | 2020-06-04 | 2024-07-09 | 10X Genomics, Inc. | Methods of enhancing spatial resolution of transcripts |
| EP4162074B1 (fr) | 2020-06-08 | 2024-04-24 | 10X Genomics, Inc. | Méthodes de détermination de marge chirurgicale et méthodes d'utilisation associées |
| EP4164796A4 (fr) | 2020-06-10 | 2024-03-06 | 10x Genomics, Inc. | Procédés de distribution de fluide |
| EP4491742A3 (fr) | 2020-09-18 | 2025-05-21 | 10x Genomics, Inc. | Appareil de manipulation d'échantillons et procédés d'enregistrement d'images |
| EP4121555A1 (fr) | 2020-12-21 | 2023-01-25 | 10X Genomics, Inc. | Procédés, compositions et systèmes pour capturer des sondes et/ou des codes à barres |
| CA3202405A1 (fr) | 2021-01-06 | 2022-07-14 | Zvi Feldman | Dispositifs d'echantillonnage a filtration |
| CN112986217A (zh) * | 2021-02-09 | 2021-06-18 | 上海英凡环保科技有限公司 | 烟气中多种重金属在线监测系统和在线监测方法 |
| WO2022256503A1 (fr) | 2021-06-03 | 2022-12-08 | 10X Genomics, Inc. | Procédés, compositions, kits et systèmes pour améliorer la capture d'analytes pour une analyse spatiale |
| EP4305195A2 (fr) | 2021-12-01 | 2024-01-17 | 10X Genomics, Inc. | Procédés, compositions et systèmes pour la détection améliorée d'analytes in situ et analyse spatiale |
| WO2023122033A1 (fr) | 2021-12-20 | 2023-06-29 | 10X Genomics, Inc. | Auto-test pour dispositif d'imagerie |
| JP2023105453A (ja) * | 2022-01-19 | 2023-07-31 | 株式会社豊田中央研究所 | 生物由来核酸回収方法、および、生物由来核酸回収装置 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3933592A (en) * | 1965-02-17 | 1976-01-20 | Hazleton Laboratories, Incorporated | Method of detecting living microorganisms |
| US4013418A (en) * | 1975-11-12 | 1977-03-22 | Vitatelt Corporation | Luminescent reaction testing |
| US4144134A (en) * | 1977-01-31 | 1979-03-13 | Vitatect Corp. | Method for detection of bacterial concentration by luminescence |
| US4385113A (en) * | 1978-03-20 | 1983-05-24 | Nasa | Rapid, quantitative determination of bacteria in water |
| US4283490A (en) * | 1978-07-28 | 1981-08-11 | Plakas Chris J | Method for detection of low level bacterial concentration by luminescence |
| EP0116454B1 (fr) * | 1983-02-11 | 1987-04-29 | National Research Development Corporation | Essai luminescent ou luminométrique amélioré |
| DK258787D0 (da) * | 1987-05-21 | 1987-05-21 | Foss Electric As N | Fremgangsmaade til bestemmelse af bakteriekoncentration i en proeve |
| US4978504A (en) * | 1988-02-09 | 1990-12-18 | Nason Frederic L | Specimen test unit |
| US4963325A (en) * | 1988-05-06 | 1990-10-16 | Hygeia Sciences, Inc. | Swab expressor immunoassay device |
| DE563858T1 (de) * | 1992-04-01 | 1994-03-03 | Nihon Millipore Kogyo K K | Verfahren zur Bestimmung der Anzahl lebender Mikroorganismen. |
| US5820826A (en) * | 1992-09-03 | 1998-10-13 | Boehringer Mannheim Company | Casing means for analytical test apparatus |
| US5624810A (en) * | 1995-01-09 | 1997-04-29 | New Horizons Diagnostics Corp. | Method for detection of surfaces contaminants |
| WO1998048280A1 (fr) * | 1997-04-24 | 1998-10-29 | Selfcare, Inc. | Dispositif de detection d'une substance dans un echantillon liquide |
-
1997
- 1997-01-15 US US08/785,833 patent/US5736351A/en not_active Expired - Fee Related
- 1997-03-25 AU AU25332/97A patent/AU2533297A/en not_active Abandoned
- 1997-03-25 WO PCT/US1997/004289 patent/WO1998032020A1/fr not_active Ceased
- 1997-03-25 CN CNB971946760A patent/CN1154847C/zh not_active Expired - Fee Related
- 1997-03-25 AT AT97916812T patent/ATE274700T1/de not_active IP Right Cessation
- 1997-03-25 EP EP04011683A patent/EP1460427A1/fr not_active Withdrawn
- 1997-03-25 JP JP53428998A patent/JP2002514916A/ja not_active Ceased
- 1997-03-25 PL PL97329003A patent/PL189724B1/pl unknown
- 1997-03-25 EP EP97916812A patent/EP0901631B1/fr not_active Expired - Lifetime
- 1997-03-25 IL IL12619297A patent/IL126192A/en not_active IP Right Cessation
- 1997-03-25 CA CA002249370A patent/CA2249370A1/fr not_active Abandoned
- 1997-03-25 DE DE69730425T patent/DE69730425T2/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002514916A (ja) | 2002-05-21 |
| WO1998032020A1 (fr) | 1998-07-23 |
| DE69730425T2 (de) | 2005-10-06 |
| EP0901631B1 (fr) | 2004-08-25 |
| CN1218553A (zh) | 1999-06-02 |
| PL189724B1 (pl) | 2005-09-30 |
| EP1460427A1 (fr) | 2004-09-22 |
| PL329003A1 (en) | 1999-03-01 |
| ATE274700T1 (de) | 2004-09-15 |
| CA2249370A1 (fr) | 1998-07-23 |
| EP0901631A4 (fr) | 2002-10-30 |
| CN1154847C (zh) | 2004-06-23 |
| DE69730425D1 (de) | 2004-09-30 |
| US5736351A (en) | 1998-04-07 |
| IL126192A0 (en) | 1999-05-09 |
| EP0901631A1 (fr) | 1999-03-17 |
| AU2533297A (en) | 1998-08-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0901631B1 (fr) | Procede de detection de contaminants | |
| US5624810A (en) | Method for detection of surfaces contaminants | |
| US6395504B1 (en) | Use of phage associated lytic enzymes for the rapid detection of bacterial contaminants | |
| US6548018B2 (en) | Apparatus for chemiluminescent assays | |
| US9068976B2 (en) | Integrated filtration bioanalyzer | |
| US7060223B2 (en) | Polymeric medium for the retention of reagent species for use in a hand-held device for the relatively rapid detection of the presence of an analyte of interest in a sample | |
| US5783399A (en) | Chemiluminescent assay methods and devices for detecting target analytes | |
| CN102325596A (zh) | 使用微粒进行的活生物负载检测 | |
| US6653147B2 (en) | Apparatus and method for chemiluminescent assays | |
| US6541194B2 (en) | Method for the detection of the presence of chemical species known to inhibit a chemiluminescent reaction | |
| US20090148870A1 (en) | Rapid Detection of Mycobacterium Tuberculosis and Antimicrobial Drug Resistance | |
| US20010046687A1 (en) | Method for the enhancement of luminescence intensity from a reaction between adenosine triphosphate (ATP) and a luciferase/luciferin reactant system | |
| JP2008501124A (ja) | マクロおよびミクロマトリックスの迅速な検出および定量方法および装置 | |
| JP6628794B2 (ja) | クロマトグラフ濃縮を使用する微生物の検出方法 | |
| JP2017530366A (ja) | クロマトグラフ濃縮を使用する検体の検出方法 | |
| US20180321202A1 (en) | Methods and devices for detecting methanol poisoning using formate oxidase | |
| MXPA98007574A (en) | Method for detection of contaminants | |
| CA2089186A1 (fr) | Diagnostic de trichomonas vaginitis par la detection de formates dans les secretions vaginales | |
| CN117405887A (zh) | 腺苷酸环化酶作为标志物在制备诊断和/或鉴别腹泻的病原体的产品中的应用 | |
| JPH01145569A (ja) | 環境分析装置 | |
| Naik et al. | A Study of Screening Tests for the Presumptive Diagnosis of Significant Bacteriuria in Urinary Tract Infections | |
| JP2002181824A (ja) | 糖抗原認識性エンドトキシン測定法 | |
| Khattak et al. | Urinalysis and Standardization |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed | ||
| MM9K | Patent not in force due to non-payment of renewal fees |