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HK68497A - Production of human parathyroid hormone from microorganisms - Google Patents

Production of human parathyroid hormone from microorganisms Download PDF

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Publication number
HK68497A
HK68497A HK68497A HK68497A HK68497A HK 68497 A HK68497 A HK 68497A HK 68497 A HK68497 A HK 68497A HK 68497 A HK68497 A HK 68497A HK 68497 A HK68497 A HK 68497A
Authority
HK
Hong Kong
Prior art keywords
sequence
plasmid
yeast
dna
parathyroid hormone
Prior art date
Application number
HK68497A
Other languages
German (de)
English (en)
Inventor
Kaare M. Gautvik
Beate Oyen Tordis
Stokke Gabrielsen Odd
Alestrom Peter
Original Assignee
Alestrom Peter
Kaare M Gautvik
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alestrom Peter, Kaare M Gautvik filed Critical Alestrom Peter
Publication of HK68497A publication Critical patent/HK68497A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/635—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Claims (5)

  1. Plasmide pour une insertion dans une levure comportant une séquence nucléotidique codant pour une production et une sécrétion d'hormone parathyroïdienne, plus particulièrement d'une hormone parathyroïdienne humaine, dans lequel la séquence nucléotidique comporte : où M = A ou C; R = A ou G; W= A ou T; S = C ou G; Y = C ou T; H = A ou C ou T; N = A ou G ou C ou T, les nucléotides 1 à 173 de ladite séquence représentant la région du promoteur de MFα1 et la séquence 5' non codante; les nucléotides 174 à 440 étant la séquence codante N-terminale de MFα1; les nucléotides 441 à 695 étant une séquence de l'HPTH, les nucléotides 696à 726 étant une séquence 3' non codante de l'HPTH provenant de pSSHPTH-10 représenté sur la figure 6, les nucléotides 727 à 732 provenant de pUC19, les nucléotides 733 à 874 étant la séquence 3' non codante de MFα1 et le signal de terminaison de la transcription.
  2. Plasmide selon la revendication 1, dans lequel la séquence de nucléotides comporte : où les nucléotides 1 à 173 de ladite séquence représentent la région du promoteur de MFα1 et la séquence 5' non codante, les nucléotides 174 à 440 sont la séquence codante N-terminale de MFα1; les nucléotides 441 à 695 sont une séquence de l'HPTH, les nucléotides 696 à 726 sont une séquence 3' non codante de l'HPTH provenant de pSSHPTH-10 représenté sur la figure 6, les nucléotides 727 à 732 proviennent de pUC19, les nucléotides 733 à 874 sont la séquence 3' non codante de MFα1 et le signal de terminaison de la transcription.
  3. Microorganisme d'une forme de levure, de manière préférentielle Saccharomyces cerevisiae, contenant le plasmide selon la revendication 1 ou 2.
  4. Procédé pour réaliser le plasmide selon la revendication 1 ou 2, comportant l'insertion de la séquence nucléotidique selon la revendication 1 ou 2, codant pour la PTH, en particulier la PTH humaine, dans un plasmide.
  5. Procédé selon la revendication 4, dans lequel l'insertion est réalisée en reclonant pSSHPTH-10, dont la carte de restriction est représentée sur la figure 6.
HK68497A 1986-10-22 1997-05-22 Production of human parathyroid hormone from microorganisms HK68497A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US92168486A 1986-10-22 1986-10-22

Publications (1)

Publication Number Publication Date
HK68497A true HK68497A (en) 1997-05-30

Family

ID=25445824

Family Applications (1)

Application Number Title Priority Date Filing Date
HK68497A HK68497A (en) 1986-10-22 1997-05-22 Production of human parathyroid hormone from microorganisms

Country Status (7)

Country Link
US (1) US6962796B1 (fr)
EP (3) EP1114865B1 (fr)
JP (3) JP2777132B2 (fr)
AU (2) AU627147B2 (fr)
DE (2) DE3789316T2 (fr)
HK (1) HK68497A (fr)
WO (1) WO1988003165A1 (fr)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE37919E1 (en) 1989-05-12 2002-12-03 The General Hospital Corporation Recombinant DNA method for production of parathyroid hormone
US5171670A (en) * 1989-05-12 1992-12-15 The General Hospital Corporation Recombinant dna method for production of parathyroid hormone
WO1991005050A1 (fr) * 1989-09-29 1991-04-18 Her Majesty In Right Of Canada As Represented By The National Research Council Of Canada Synthese de l'hormone parathyroïde humaine mature
DE69111371T2 (de) * 1990-09-28 1996-03-14 Takeda Chemical Industries Ltd Synthetisches Gen für menschliches Parathyroidhormon.
DE69219242T2 (de) * 1991-08-07 1997-09-25 Takeda Chemical Industries Ltd Muteine des menschlichen Parathormons und ihre Herstellung
JPH05271279A (ja) * 1991-08-07 1993-10-19 Takeda Chem Ind Ltd ヒト副甲状腺ホルモンムテインおよびその製造法
US5382658A (en) * 1992-04-03 1995-01-17 Allelix Biopharmaceuticals Inc. Stability-enhanced variants of parathyroid hormone
CA2098639A1 (fr) * 1992-06-19 1993-12-20 K. Anne Kronis Variantes de l'hormone parathyroidienne osteostimulantes et non vasoactives
US5830853A (en) 1994-06-23 1998-11-03 Astra Aktiebolag Systemic administration of a therapeutic preparation
US6632456B1 (en) 1993-06-24 2003-10-14 Astrazeneca Ab Compositions for inhalation
US6794357B1 (en) 1993-06-24 2004-09-21 Astrazeneca Ab Compositions for inhalation
DE69530325T2 (de) 1994-12-22 2004-02-19 Astrazeneca Ab Aerosol-arzneiformulierungen
US6524557B1 (en) 1994-12-22 2003-02-25 Astrazeneca Ab Aerosol formulations of peptides and proteins
ES2258371B1 (es) * 2000-08-31 2007-05-16 Dong Kook Pharmaceutical Co. Ltd. Levadura transformante productora de hormona paratiroidea humana y procedimiento para producir la hormona.
ES2372998T3 (es) * 2007-04-05 2012-01-30 Symbiotec Gesellschaft Zur Forschung Und Entwicklung Auf Dem Gebiet Der Biotechnologie Mbh Histonas bis-met.
EP1985628A1 (fr) * 2007-04-05 2008-10-29 SYMBIOTEC Gesellschaft zur Forschung und Entwicklung auf dem Gebiet der Biotechnologie mbH Histones bis-met

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3886132A (en) 1972-12-21 1975-05-27 Us Health Human parathyroid hormone
US4237224A (en) 1974-11-04 1980-12-02 Board Of Trustees Of The Leland Stanford Jr. University Process for producing biologically functional molecular chimeras
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4264731A (en) 1977-05-27 1981-04-28 The Regents Of The University Of California DNA Joining method
US4366246A (en) 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4425437A (en) 1979-11-05 1984-01-10 Genentech, Inc. Microbial polypeptide expression vehicle
US4338397A (en) 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
US4383066A (en) 1980-04-15 1983-05-10 Mitsubishi Gas Chemical Company, Inc. Polyphenylene ether resin composition
JPS5825439B2 (ja) 1980-12-30 1983-05-27 株式会社林原生物化学研究所 ヒト副甲状腺ホルモンの製造方法
US4394443A (en) 1980-12-18 1983-07-19 Yale University Method for cloning genes
US4624926A (en) 1981-01-02 1986-11-25 The Research Foundation Of State University Of New York Novel cloning vehicles for polypeptide expression in microbial hosts
NO163060C (no) 1981-03-13 1990-03-28 Damon Biotech Inc Fremgangsmaate for fremstilling av substanser som dannes av levende celler som produserer interferoner eller antistoffer.
US4532207A (en) 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
US4546082A (en) 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
EP0119247A1 (fr) * 1982-09-15 1984-09-26 Immuno Nuclear Corporation Production de proteines mures dans une levure transformee
DE3312928A1 (de) * 1983-04-11 1984-11-22 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Human-parathormon produzierende hybridvektoren und human-parathormongen
NZ207926A (en) 1983-04-25 1988-04-29 Genentech Inc Use of yeast #a#-factor to assist in expression of proteins heterologus to yeast
US4588684A (en) 1983-04-26 1986-05-13 Chiron Corporation a-Factor and its processing signals
CA1213537A (fr) 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Methode d'expression de polypeptides
US5010010A (en) 1986-10-22 1991-04-23 Selmer-Sande, A.S. Production of human parathyroid hormone from microorganisms
US5420242A (en) * 1986-10-22 1995-05-30 Kaare M. Gautvik Production of human parathyroid hormone from microorganisms
USRE37919E1 (en) * 1989-05-12 2002-12-03 The General Hospital Corporation Recombinant DNA method for production of parathyroid hormone
US5171670A (en) 1989-05-12 1992-12-15 The General Hospital Corporation Recombinant dna method for production of parathyroid hormone

Also Published As

Publication number Publication date
JPH1014587A (ja) 1998-01-20
EP0604706A1 (fr) 1994-07-06
EP1114865A1 (fr) 2001-07-11
AU627147B2 (en) 1992-08-20
AU2851692A (en) 1993-04-08
JP2777132B2 (ja) 1998-07-16
JP2828427B2 (ja) 1998-11-25
DE3789316T2 (de) 1994-08-25
WO1988003165A1 (fr) 1988-05-05
US6962796B1 (en) 2005-11-08
DE3752375T8 (de) 2005-03-10
JPH02501108A (ja) 1990-04-19
AU8104887A (en) 1988-05-25
JPH09110899A (ja) 1997-04-28
AU665034B2 (en) 1995-12-14
DE3789316D1 (de) 1994-04-14
EP1114865B1 (fr) 2004-01-21
EP0383751B1 (fr) 1994-03-09
DE3752375T2 (de) 2004-10-21
DE3752375D1 (de) 2004-02-26
JP2834111B2 (ja) 1998-12-09
EP0383751A1 (fr) 1990-08-29

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PC Patent ceased (i.e. patent has lapsed due to the failure to pay the renewal fee)