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GR20120100368A - Direct laser immobilization of biomolecules on rough substrates - Google Patents

Direct laser immobilization of biomolecules on rough substrates

Info

Publication number
GR20120100368A
GR20120100368A GR20120100368A GR20120100368A GR20120100368A GR 20120100368 A GR20120100368 A GR 20120100368A GR 20120100368 A GR20120100368 A GR 20120100368A GR 20120100368 A GR20120100368 A GR 20120100368A GR 20120100368 A GR20120100368 A GR 20120100368A
Authority
GR
Greece
Prior art keywords
biomolecules
immobilization
rough
substrates
direct laser
Prior art date
Application number
GR20120100368A
Other languages
Greek (el)
Inventor
Χρηστος Γεωργιου Μπουτοπουλος
Ελευθεριος Τουλουπακης
Giuseppe Rodio
Ιωαννα Σταυρου Ζεργιωτη
Original Assignee
Εθνικο Μετσοβιο Πολυτεχνειο,
Biosensor Srl.,
Ιωαννα Σταυρου Ζεργιωτη
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Εθνικο Μετσοβιο Πολυτεχνειο,, Biosensor Srl.,, Ιωαννα Σταυρου Ζεργιωτη filed Critical Εθνικο Μετσοβιο Πολυτεχνειο,
Priority to GR20120100368A priority Critical patent/GR20120100368A/en
Publication of GR20120100368A publication Critical patent/GR20120100368A/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C23COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23CCOATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C14/00Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material
    • C23C14/04Coating on selected surface areas, e.g. using masks
    • C23C14/048Coating on selected surface areas, e.g. using masks using irradiation by energy or particles
    • CCHEMISTRY; METALLURGY
    • C23COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23CCOATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C14/00Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material
    • C23C14/04Coating on selected surface areas, e.g. using masks
    • CCHEMISTRY; METALLURGY
    • C23COATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; CHEMICAL SURFACE TREATMENT; DIFFUSION TREATMENT OF METALLIC MATERIAL; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL; INHIBITING CORROSION OF METALLIC MATERIAL OR INCRUSTATION IN GENERAL
    • C23CCOATING METALLIC MATERIAL; COATING MATERIAL WITH METALLIC MATERIAL; SURFACE TREATMENT OF METALLIC MATERIAL BY DIFFUSION INTO THE SURFACE, BY CHEMICAL CONVERSION OR SUBSTITUTION; COATING BY VACUUM EVAPORATION, BY SPUTTERING, BY ION IMPLANTATION OR BY CHEMICAL VAPOUR DEPOSITION, IN GENERAL
    • C23C14/00Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material
    • C23C14/22Coating by vacuum evaporation, by sputtering or by ion implantation of the coating forming material characterised by the process of coating
    • C23C14/24Vacuum evaporation
    • C23C14/28Vacuum evaporation by wave energy or particle radiation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Mechanical Engineering (AREA)
  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

A method for direct immobilization of biomolecules onto rough solid substrates by means of laser transfer is enclosed. The method involves the use of laser pulses for transferring biomolecules from a donor substrate onto the opposite positioned rough surface of a receiving substrate. The high kinetic energy of the transferred biomaterials enhances their physical adsorption on rough substrates. As a result, an immobilization process free of linkers takes place. The method is of particular utility in producing devices for biological and chemical sensing applications.
GR20120100368A 2012-07-11 2012-07-11 Direct laser immobilization of biomolecules on rough substrates GR20120100368A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20120100368A GR20120100368A (en) 2012-07-11 2012-07-11 Direct laser immobilization of biomolecules on rough substrates

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20120100368A GR20120100368A (en) 2012-07-11 2012-07-11 Direct laser immobilization of biomolecules on rough substrates

Publications (1)

Publication Number Publication Date
GR20120100368A true GR20120100368A (en) 2014-02-24

Family

ID=47563536

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20120100368A GR20120100368A (en) 2012-07-11 2012-07-11 Direct laser immobilization of biomolecules on rough substrates

Country Status (1)

Country Link
GR (1) GR20120100368A (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003056320A2 (en) * 2001-12-28 2003-07-10 Foundation For Research And Technology Hellas (F.O.R.T.H) Fabrication of biopolymer patterns by means of laser transfer

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003056320A2 (en) * 2001-12-28 2003-07-10 Foundation For Research And Technology Hellas (F.O.R.T.H) Fabrication of biopolymer patterns by means of laser transfer

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ARNOLD B CRAIG ET AL: "mrs bulletin", MRS BULLETIN, vol. 32, 1 January 2007 (2007-01-01), pages 23 - 31, XP055062191 *
BOUTOPOULOS CHRISTOS ET AL: "Direct laser immobilization of photosynthetic material on screen printed electrodes for amperometric biosensor", APPLIED PHYSICS LETTERS, AIP, AMERICAN INSTITUTE OF PHYSICS, MELVILLE, NY, US, vol. 98, no. 9, 2 March 2011 (2011-03-02), pages 93703 - 93703, XP012140167, ISSN: 0003-6951, DOI: 10.1063/1.3562297 *
CHATZIPETROU M ET AL: "Direct laser printing of oligonucleotides for the fabrication of a label-free biosensor", PROCEDIA ENGINEERING, vol. 25, 7 September 2011 (2011-09-07), pages 851 - 855, XP028436667, ISSN: 1877-7058, [retrieved on 20120108], DOI: 10.1016/J.PROENG.2011.12.209 *
ELEFTHERIOS TOULOUPAKIS ET AL: "A photosynthetic biosensor with enhanced electron transfer generation realized by laser printing technology", ANALYTICAL AND BIOANALYTICAL CHEMISTRY, SPRINGER, BERLIN, DE, vol. 402, no. 10, 3 February 2012 (2012-02-03), pages 3237 - 3244, XP035029180, ISSN: 1618-2650, DOI: 10.1007/S00216-012-5771-7 *
MARTIN GRUENE ET AL: "Dispensing pico to nanolitre of a natural hydrogel by laser-assisted bioprinting", BIOMEDICAL ENGINEERING ONLINE, BIOMED CENTRAL LTD, LONDON, GB, vol. 10, no. 1, 7 March 2011 (2011-03-07), pages 19, XP021098560, ISSN: 1475-925X, DOI: 10.1186/1475-925X-10-19 *

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Legal Events

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ML Lapse due to non-payment of fees

Effective date: 20150204