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GR20000100255A - DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZING POLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO.... - Google Patents

DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZING POLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO....

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Publication number
GR20000100255A
GR20000100255A GR20000100255A GR2000100255A GR20000100255A GR 20000100255 A GR20000100255 A GR 20000100255A GR 20000100255 A GR20000100255 A GR 20000100255A GR 2000100255 A GR2000100255 A GR 2000100255A GR 20000100255 A GR20000100255 A GR 20000100255A
Authority
GR
Greece
Prior art keywords
conjugated
immunoenzymic
polyiodothyronine
triiodothyronine
thyroxine
Prior art date
Application number
GR20000100255A
Other languages
Greek (el)
Other versions
GR1004119B (en
Inventor
Χριστινα Καραπιττα
Αριστοτελης Ξενακης
Θεοδωρος Σωτηρουδης
Original Assignee
Αυτοματοι Αναλυτες Και Διαγνωστικα Αντιδραστηρια Medicon Hellas..
Εθνικο Ιδρυμα Ερευνων (Ν.Π.Ι.Δ.)
Χριστινα Καραπιττα
Αριστοτελης Ξενακης
Θεοδωρος Σωτηρουδης
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Αυτοματοι Αναλυτες Και Διαγνωστικα Αντιδραστηρια Medicon Hellas.., Εθνικο Ιδρυμα Ερευνων (Ν.Π.Ι.Δ.), Χριστινα Καραπιττα, Αριστοτελης Ξενακης, Θεοδωρος Σωτηρουδης filed Critical Αυτοματοι Αναλυτες Και Διαγνωστικα Αντιδραστηρια Medicon Hellas..
Priority to GR20000100255A priority Critical patent/GR1004119B/en
Publication of GR20000100255A publication Critical patent/GR20000100255A/en
Publication of GR1004119B publication Critical patent/GR1004119B/en

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

he invention describes a new immunoenzymic method for the quantification of thyroxine and triiodothyronine in human serum. The said hormones bond with glycogen phosphorylase b deriving from rabbit muscle tissue.The method is based on the inhibition of the activity of phosphorylase b when it is conjugated with poliodothyronines and its activation when the conjugated hormone bonds with the appropriate antibody against the polyiodothyronine.In an aqueous solution that contains an unidentifiedconcentration of hormone, conjugated phosphorylase and antibody, the free and the conjugated hormone molecules will be competing for the antibody and thus, the enzymic activation caused by the antibody will decrease. The concentration of the hormonein the unknown sample is determined with the use of a standard chart depicting the activation of enzymic activity in relation to known hormone concentration.
GR20000100255A 2000-07-26 2000-07-26 DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZINGPOLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO.... GR1004119B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GR20000100255A GR1004119B (en) 2000-07-26 2000-07-26 DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZINGPOLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO....

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GR20000100255A GR1004119B (en) 2000-07-26 2000-07-26 DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZINGPOLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO....

Publications (2)

Publication Number Publication Date
GR20000100255A true GR20000100255A (en) 2002-09-06
GR1004119B GR1004119B (en) 2003-01-23

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GR20000100255A GR1004119B (en) 2000-07-26 2000-07-26 DEVELOPMENT OF A NEW HOMOGENEOUS IMMUNOENZYMIC METHOD FOR THE PRODUCTION OF A CLINICAL LABORATORY TEST SYSTEM (kit) FOR THE QUANTIFICATION OF THYROXINE AND TRIIODOTHYRONINE IN HUMAN SERUM, UTILIZINGPOLYIODOTHYRONINE CONJUGATED WITH GLYCOGEN PHOSPHO....

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
EP0254430A2 (en) * 1986-06-26 1988-01-27 Ortho Diagnostic Systems Inc. Light-scattering immunoassay system
US4847195A (en) * 1985-05-31 1989-07-11 Syntex (U.S.A.) Inc. Glucose-6-phosphate dehydrogenase conjugates useful in polyiodothyronine assays
US4959306A (en) * 1986-11-28 1990-09-25 Sclavo, Inc. Labeling design for a binding assay reagent
US5273885A (en) * 1992-07-31 1993-12-28 Syntex (U.S.A.) Inc. Conjugates of monophenyl thyroid analogs useful in assays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US4847195A (en) * 1985-05-31 1989-07-11 Syntex (U.S.A.) Inc. Glucose-6-phosphate dehydrogenase conjugates useful in polyiodothyronine assays
EP0254430A2 (en) * 1986-06-26 1988-01-27 Ortho Diagnostic Systems Inc. Light-scattering immunoassay system
US4959306A (en) * 1986-11-28 1990-09-25 Sclavo, Inc. Labeling design for a binding assay reagent
US5273885A (en) * 1992-07-31 1993-12-28 Syntex (U.S.A.) Inc. Conjugates of monophenyl thyroid analogs useful in assays

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAWKINS ROBERT C: "Furosemide interference in newer free thyroxine assays.", CLINICAL CHEMISTRY, vol. 44, no. 12, December 1998 (1998-12-01), pages 2550 - 2551, XP002175019, ISSN: 0009-9147 *
KARAPITTA CHRISTINA D ET AL: "A new homogeneous enzyme immunoassay for thyroxine using glycogen phosphorylase b-thyroxine conjugates.", CLINICA CHIMICA ACTA, vol. 308, no. 1-2, June 2001 (2001-06-01), pages 99 - 106, XP001020702, ISSN: 0009-8981 *
KARAPITTA CHRISTINA D ET AL: "Homogeneous enzyme immunoassay for triiodothyronine in serum.", CLINICAL CHEMISTRY, vol. 47, no. 3, March 2001 (2001-03-01), pages 569 - 574, XP001020704, ISSN: 0009-9147 *

Also Published As

Publication number Publication date
GR1004119B (en) 2003-01-23

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