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GB2417961B - Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method - Google Patents

Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method

Info

Publication number
GB2417961B
GB2417961B GB0525046A GB0525046A GB2417961B GB 2417961 B GB2417961 B GB 2417961B GB 0525046 A GB0525046 A GB 0525046A GB 0525046 A GB0525046 A GB 0525046A GB 2417961 B GB2417961 B GB 2417961B
Authority
GB
United Kingdom
Prior art keywords
cryopreservation
stem cells
derived stem
human blastocyst
vitrification method
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB0525046A
Other versions
GB2417961A (en
GB2417961A8 (en
GB2417961B8 (en
GB0525046D0 (en
Inventor
Anita Sjoegren
Eva Sjoegren-Jansson
Sven Enerbaeck
Peter Eriksson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio Europe AB
Original Assignee
Cellartis AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellartis AB filed Critical Cellartis AB
Publication of GB0525046D0 publication Critical patent/GB0525046D0/en
Publication of GB2417961A publication Critical patent/GB2417961A/en
Publication of GB2417961B publication Critical patent/GB2417961B/en
Application granted granted Critical
Publication of GB2417961A8 publication Critical patent/GB2417961A8/en
Publication of GB2417961B8 publication Critical patent/GB2417961B8/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0623—Stem cells
    • A01N1/02—
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Veterinary Medicine (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Neurology (AREA)
  • Neurosurgery (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Transplantation (AREA)
  • Reproductive Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
GB0525046A 2003-05-08 2004-05-10 Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method Expired - Fee Related GB2417961B8 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US46932003P 2003-05-08 2003-05-08
DKPA200300700 2003-05-08
DKPA200300983 2003-06-27
DKPA200300984 2003-06-27
PCT/EP2004/005031 WO2004098285A2 (en) 2003-05-08 2004-05-10 Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method

Publications (5)

Publication Number Publication Date
GB0525046D0 GB0525046D0 (en) 2006-01-18
GB2417961A GB2417961A (en) 2006-03-15
GB2417961B true GB2417961B (en) 2008-03-12
GB2417961A8 GB2417961A8 (en) 2008-05-09
GB2417961B8 GB2417961B8 (en) 2008-05-09

Family

ID=33437113

Family Applications (1)

Application Number Title Priority Date Filing Date
GB0525046A Expired - Fee Related GB2417961B8 (en) 2003-05-08 2004-05-10 Cryopreservation of human blastocyst-derived stem cells by use of a closed straw vitrification method

Country Status (9)

Country Link
EP (1) EP1624751A2 (en)
JP (1) JP2006525006A (en)
KR (1) KR20060032953A (en)
AU (1) AU2004237425B2 (en)
CA (1) CA2524929C (en)
GB (1) GB2417961B8 (en)
IL (1) IL171595A (en)
SE (1) SE0502397L (en)
WO (1) WO2004098285A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005072523A2 (en) 2004-02-02 2005-08-11 I.M.T. Interface Multigrad Technology Ltd. Biological material and methods and solutions for preservation thereof
US8196416B2 (en) 2004-02-02 2012-06-12 Core Dynamics Limited Device for directional cooling of biological matter
CA2569485C (en) 2004-06-02 2015-08-18 Es Cell International Pte Ltd. Cell preservation method
WO2005120591A1 (en) 2004-06-07 2005-12-22 I.M.T. Interface Multigrad Technology Ltd. Method for sterilization of biological preparations
EP1778007A1 (en) 2004-08-12 2007-05-02 I.M.T. Interface Multigrad Technology Ltd. Method and apparatus for freezing or thawing of a biological material
MY142987A (en) 2005-06-08 2011-02-14 Hayashibara Biochem Lab Solution for tissue adhesion prevention and method for tissue adhesion prevention
EP1909565A2 (en) * 2005-08-03 2008-04-16 Interface Multigrad Technology (IMT) Ltd. Somatic cells for use in cell therapy
CA2657065A1 (en) 2006-07-13 2008-01-17 Cellartis Ab A novel population of multipotent cardiac precursor cells derived from human blastocysts derived stem cells
WO2008011070A2 (en) * 2006-07-19 2008-01-24 Reprocure, Llc A method of oocyte cryopreservation including piercing the zona pellucida prior to vitrification
US8785193B2 (en) 2006-09-14 2014-07-22 The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Dissection tool and methods of use
CA2693481A1 (en) 2007-07-20 2009-01-29 Cellartis Ab A novel population of hepatocytes derived via definitive endoderm (de-hep) from human blastocysts derived stem cells
KR101849336B1 (en) 2007-10-12 2018-04-16 아스텔라스 인스티튜트 포 리제너러티브 메디슨 Improved methods of producing rpe cells and compositions of rpe cells
EP2156735A1 (en) * 2008-08-13 2010-02-24 Dr. H. Zech GmbH Method and instrument for vitrification and storing of biological specimen
US8956867B2 (en) 2008-11-07 2015-02-17 Wisconsin Alumni Research Foundation Method for culturing stem cells
NZ603913A (en) * 2010-05-07 2015-09-25 Univ North Carolina Method of engrafting cells from solid tissues
KR101358973B1 (en) * 2010-11-24 2014-02-11 중앙대학교 산학협력단 Method of cryopreservation of spermatogonial stem cells using trehalose
TWI812867B (en) 2011-11-30 2023-08-21 安斯泰來再生醫藥協會 Methods of generating mesenchymal stromal cells using hemangioblasts
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
CA2876499C (en) 2012-07-12 2021-10-26 Imstem Biotechnology, Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
KR101509295B1 (en) * 2012-11-07 2015-04-07 경상대학교산학협력단 Paste an embryo in the vitrification straw
BR102016003292B1 (en) 2015-07-14 2022-08-09 Genus Plc METHOD TO PREPARE IN VITRO PRODUCED UNGULATE EMBRYO, DEVICE AND METHODS OF FREEZING AND THawING UNGULATE EMBRYOS
WO2017099865A1 (en) 2015-12-07 2017-06-15 Coopersurgical, Inc. Low temperature specimen carriers and related methods
CN110214776A (en) * 2019-07-01 2019-09-10 安徽农业大学 A kind of cryopreservation and thawing method of pig blastocyst based on straw freezing Tip carrier
CA3182658A1 (en) 2020-05-08 2021-11-11 Gallant Pet, Inc. Uterine-derived regenerative cell compositions and uses thereof
CN120458084B (en) * 2025-07-09 2025-09-26 山东奥克斯畜牧种业有限公司 Thawing solution for bovine vitrification frozen in-vitro embryo and application method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999011121A1 (en) * 1997-09-03 1999-03-11 Danish Institute Of Agricultural Sciences Method and auxiliaries for cryopreservation of biological material such as egg cells
WO2000021365A1 (en) * 1998-10-14 2000-04-20 Forest Katrina T Method for vitrification of a biological specimen
EP1131998A1 (en) * 2000-03-08 2001-09-12 Director-General Of National Institute Of Animal Industry, Ministry Of Agriculture, Forestry And Fisheries Cell-cryopreservation method

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000027995A1 (en) * 1998-11-09 2000-05-18 Monash University Embryonic stem cells

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999011121A1 (en) * 1997-09-03 1999-03-11 Danish Institute Of Agricultural Sciences Method and auxiliaries for cryopreservation of biological material such as egg cells
WO2000021365A1 (en) * 1998-10-14 2000-04-20 Forest Katrina T Method for vitrification of a biological specimen
EP1131998A1 (en) * 2000-03-08 2001-09-12 Director-General Of National Institute Of Animal Industry, Ministry Of Agriculture, Forestry And Fisheries Cell-cryopreservation method

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Biology of Reproduction, Vol 65, 2001, S Kaidi et al "Effect of conventional controlled-rate freezing and vitrification on morphology and metabolism of bovine blastocysts produced in vitro" 1127-1134 *
Cryo Letters, Vol 19, 1998, G Vajta et al "Sterile application of the Open Pulled Straw (OPS) vitrification method" 389-392 *
Human Reproduction, SU Chen et al "Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws" 2598-2603 *
Human reproduction, Vol 16, 2001, BE Reubinoff et al "EFFECTIVE CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS BY THE OPEN PULLED STRAW VITRIFICATION METHOD" 2187-2194 *
Human Reproduction, Vol 16, 2001, SU Chen et al "Vitrification of mouse oocytes using closed pulled straws (CPS) achieves a high survival and preserves good patterns of meiotic spindles, compared with conventional straws, open pulled straws (OPS) and grids" 2350-2356 *
J Reprod & Fertil, Vol. 96, 1992, CA Valdez et al, "SUCCESFUL CRYOPRESERVATION OF MOUSE BLASTOCYSTS USING A NEW VITRIFICATION SOLUTION" 793-802 *
Theriogenology, Vol. 58, 2002, M Lopez-Bejar et al "Nonequilibrium cryopreservation of rabbit embryos using a modified (sealed) open pulled straw procedure" 1541-1552 *

Also Published As

Publication number Publication date
WO2004098285A3 (en) 2005-01-06
GB2417961A (en) 2006-03-15
JP2006525006A (en) 2006-11-09
GB2417961A8 (en) 2008-05-09
CA2524929C (en) 2015-01-13
GB2417961B8 (en) 2008-05-09
AU2004237425B2 (en) 2010-03-04
CA2524929A1 (en) 2004-11-18
IL171595A (en) 2012-07-31
SE0502397L (en) 2005-12-29
AU2004237425A1 (en) 2004-11-18
KR20060032953A (en) 2006-04-18
EP1624751A2 (en) 2006-02-15
GB0525046D0 (en) 2006-01-18
WO2004098285A2 (en) 2004-11-18

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20160510