GB2495536A - Bioanalytical device with mixing device - Google Patents
Bioanalytical device with mixing device Download PDFInfo
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- GB2495536A GB2495536A GB1117736.7A GB201117736A GB2495536A GB 2495536 A GB2495536 A GB 2495536A GB 201117736 A GB201117736 A GB 201117736A GB 2495536 A GB2495536 A GB 2495536A
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- immunoadsorbent
- immunoreactants
- zone
- mobile phase
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- 239000011324 bead Substances 0.000 claims abstract description 26
- 239000000376 reactant Substances 0.000 claims abstract description 20
- 238000003018 immunoassay Methods 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 12
- 238000006243 chemical reaction Methods 0.000 claims abstract description 11
- 230000003321 amplification Effects 0.000 claims abstract description 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 6
- 230000005484 gravity Effects 0.000 claims abstract description 3
- 239000012491 analyte Substances 0.000 claims description 29
- 238000001514 detection method Methods 0.000 claims description 15
- 210000004369 blood Anatomy 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
- 238000004458 analytical method Methods 0.000 claims description 6
- 239000011541 reaction mixture Substances 0.000 claims description 6
- 239000013566 allergen Substances 0.000 claims description 5
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 108020004707 nucleic acids Proteins 0.000 claims description 3
- 150000007523 nucleic acids Chemical class 0.000 claims description 3
- 102000039446 nucleic acids Human genes 0.000 claims description 3
- 230000005670 electromagnetic radiation Effects 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 238000005381 potential energy Methods 0.000 claims description 2
- 230000010399 physical interaction Effects 0.000 claims 2
- 239000003085 diluting agent Substances 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 5
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 17
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 4
- 239000010931 gold Substances 0.000 description 4
- 229910052737 gold Inorganic materials 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 3
- 238000013019 agitation Methods 0.000 description 3
- 239000000428 dust Substances 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000036046 immunoreaction Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
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- 229910052742 iron Inorganic materials 0.000 description 1
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
- B01F25/52—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle with a rotary stirrer in the recirculation tube
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
- B01F25/51—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle in which the mixture is circulated through a set of tubes, e.g. with gradual introduction of a component into the circulating flow
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
- B01F25/54—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle provided with a pump inside the receptacle to recirculate the material within the receptacle
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F33/00—Other mixers; Mixing plants; Combinations of mixers
- B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
- B01F33/452—Magnetic mixers; Mixers with magnetically driven stirrers using independent floating stirring elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
An analytical device for performing immunoassay and polymerase chain reactions (PCR) where the reactants are mixed by repeated propulsion around a circuit (1) by an inert bead containing a magnetic core (3), driven by a rotating magnet 6. Other means of propelling the reactants such as a pump, turbine, pressure change chemical reaction or the effect of gravity are also disclosed. In the case of immunoassay the rapid recirculation allows increased rates of collision between reactants without the need for physical shaking of the device, increasing the rate of reaction. In the case of PCR, the recirculation of the PCR mix passes through zones situated along the circuit (1) each zone exhibiting a different temperatures to the preceding zone thereby allowing the amplification of DNA by PCR.
Description
LABORATORY APPARATUS FOR RESEARCH AND DIAGNOSIS
The present invention relates to apparatus for the diagnosis, monitoring and research of clinical disease.
In particular, the present invention relates to immunoassay, nucleic acid detection and polynicrase chain reaction (PCR) apparatus suited for both point-of-care (POC) and laboratory situations.
In order for reactions to rcach equilibrium in a short pcriod of time most mcdical laboratory analytical techniques such as ELISA and automated commercial enzyme immunoassay systems employ a means of agitation of the reactants by physical shaking of the reaction vessel such as a test tube or microtitre plate. In the case of PCR, there is a need to sequentially expose reactants to different temperatures in order for amplification of target DNA to take place.
Some immunoassay formats, such as the immunochromatographic strips commonly found in POC tests, do not employ agitation of the reactants. High lateral flow rates are employed in immunochromatographic techniques to provide rapid results.
However, high lateral flow rates along thcse thin membranes gives inferior analytical scnsitivity due to thc rapid passage of analytc through the detector zonc without binding to the immunoadsorbcnt. Similarly, particulate and viscous fluids, such as plasma or serum, flow slower through the membranes than aqueous fluids such as urine with consequential reduced sensitivity of the device, longer times to result and the need for a centrifugation or filtration step to remove blood cells which can block the flow of reactants through the membrane giving an erroneous result.
To overcome these limitations, the present invention proposes a circuit through which immunoreactants can rapidly and repeatedly circulate allowing interactions between reactants to reach equilibrium quicker, resulting in improved analytical sensitivity and faster result times. Similarly, with PCR, the circuit containing sample DNA, primers, polymcrasc enzymes and bases circulate through zones along the circuit of different temperatures allowing PCR to take place at high speed eliminating the lag due to heating and cooling times.
In the present invention, all the analyte remains available to the immunoreaetants during the assay procedure. Reaction rates are increased due to the rapid recirculation of the analyte through the immunoreactants which increases the probability of interaction between analytes and immunoreactants without the need for physical shaking of the device. The immunoreactants could also comprise of intact or organism parts that take part in the adsorption or detection of the analyte.
High dose hook effects associated with non-sequential immunometric assays, particulate and or viscous samples, slow reaction rates and non-specific binding may be overcome by incorporating wash steps and peimitting the analyte, reactants and wash solutions to repeatedly circulate over the immunoadsorbent in sequence.
The immunoassay analytical test apparatus according to the invention comprises: (a) a zone for receiving a sample containing an analyte; b) a zone for receiving a mobile phase, which zone may be the same as the sample receiving zone, or different thereto; (c) a zone for absorbing excess reactants and wash solutions (d) a detection means for permitting detection of said analyte by immunoreaction; and (e) a means for circulating immunoreaetants through the mobile phase containing the reactants.
The immunoadsorbent according to the present invention comprises of macroscopic particles on to which materials which bind to the analyte are adsorbed and circdated through the immunoreactants during the test procedure.
It is a preferred feature of the present invention that the immunoadsorbent is repeatedly circulated through a mobile phase containing the analyte and immunoreactants.
According to a first embodiment of the present invention this may be achieved by providing a means of repeated circulation of the immunoadsorbent and analyte through a mobile phase containing the immunoreactants.
Therefore, according to a first embodiment of the present invention the test apparatus comprises: (a) a zone for receiving a sample containing an analyte; (b) a zone for receiving a mobile phase, which zone may be the same as the sample receiving zone, or different thereto; (c) a means of circulating the immunoadsorbent through the mobile phase containing the immunoreactants, analyte and wash solutions; and (d) a means of delivering the immunoadsorbent to the detection zone.
To operate the apparatus according to the present invention, a user provides a sample containing the analyte, for example, a sample of blood. The blood or other sample is then allowed to be taken up by the sample receiving zone. The sample receiving zone preferably comprises of a suitable blood filter to aid with the separation of plasma from the whole blood sample. Mobile phase may then be applied preferably upstream or directly on top of the blood sample. Aliquots of mobile phase, which may also contain one or more immunoreactants that allow detection of the analyte, may be applied directly or indirectly to the mobile phase receiving zone, which may be the same or different area to the sample receiving zone, from a separate container holding mobile phase, for example, from one or more droppcr bottles. Typical means for repeated circulation of reactants within the circuit include the action of one or more applications of a magnetic field, pump, turbine, gravity, electric motor, friction power, potential energy, temperature gradient, pressure gradient, chemical reaction, osmotic pressure, electromagnetic radiation, electric current or sound waves.
An example of the invention will now be described by referring to the accompanying drawi ags: Figure 1 shows a circuit within the apparatus suitable for immunoassay Figure 2 shows a cross section of an apparatus suitable for immunoassay Figure 3 shows a circuit within an apparatus suitable for PCR
Example 1
An inimunoadsorbent (2), comprised of house dust mite allergens adsorbed to one or more macroscopic polystyrene beads I to 5mm in diameter arranged as a train' which may be separated from each other by inert beads to aid identification of the immunoadsorbent beads. The train of beads are rapidly and continuously propelled around a continuous "track" (1) containing serum from a patient and gold labelled anti-human IgE antibodies for a fixed period of time. The rapid circulation of the train of immunoadsorbent beads through the mobile phase containing the immunoreactants and analyte increasing the rate of reaction. During the rapid recirculation of immuoreactants and analyte, human IgE antibodies specific for house dust mite aHergens will bind to the allergen adsorbed to the polystyrene bead. Simultaeneously, the gold labelled anti-human IgE antibodies will bind to the bound human IgE antibodies. The quantity of house dust mite allergen specific IgE antibodies will be proportional to the intensity of the pink colouration of the bead due to the binding of the gold labelled antibodies. Similarly, enzyme, fluorescent and other detection systems common to those skilled in the art of immunoassays may be substituted for the gold label.
In a preferred embodiment of the invention a "chaser bead" (3) containing a core of metal, such as iron, which can be forced to travel around the circuit track by a rotating magnet (6) about an axis (7) causing the immunoadsorbent beads to be propelled around the track containing the immunoreactants and analyte. Absorbent pads (5) encase the apparatus which absorb wash solutions and used reactants ejected through pores in the apparatus (8).
In a further preferred embodiment of the invention the train of beads comprises multiple beads each with differing specificity allowing different analytes to be detected simultaneously from the same sample.
In a further embodiment the train of beads comprises of additional beads that are adsorbed with variable amounts of reagents to provide a calibration curve and on board' controls.
A further embodiment of the present invention includes a filter (4) for the removal of unwanted particulate components such as red blood cells from the patient sample.
According to a further embodiment of the present invention the immunoreactants repeatedly circulate over a stationary immunoadsorbent. Said immunoadsorbent comprises of a stationary surface onto which materials which bind to the analytc are adsorbed. Analyte and immunoreactants for producing a detectable signal are circulated over the immunoadsorbent propelled by onc or more "chaser beads" or a turbine.
Therefore, according to said ifirther embodiment the test apparatus comprises: (a) a zone for receiving a sample containing an analyte; (b) a zone for receiving a mobile phase, which zone may be the same as the sample receiving zone, or different thereto; (c) a means of circulating the immunoreactants in the mobile phase over the stationary immunoadsorbent; and (d) a detection means for permitting detection of said analyte by immunoreaction.
Reaction kinetics may be further enhanced by increasing the temperature of the immunoreaetants with a source of heat, surface modification of the beads such as dimples or protuberances to enhance physical mixing and baffles along the circuit to provide ffirther agitation to the flow of immunoreactants in the apparatus.
Example 2
PCR requires the repeated sequential heating and cooling of the PCR mix to promote amplification of the DNA sequence of interest. In a further embodiment of the invention, the circuit containing the typical PCR mix of buffers, enzymes, bases, primer sequence and sample DNA is flanked with one or more distinct and separate zones of differing temperatures typical for PCR. For example, temperature zone 1 (9), temperature zone 2(10), temperature zone 3 (11). A bolus(12) of the PCR mix constructed previous to the introduction to the circulation channel (1) or with the reagents added sequentially, is propelled by one or more "chaser beads" (3), proximal or proximal and distal to the PCR mix bolus, to repeatedly circulate within the apparatus causing the reactants to be sequentially exposed to regions of differing temperature pronloting amplification of the desired DNA sequence. The duration of exposure of the bolus of reaction mix to the different temperatures being dctcrm[ned by the zone length and length of time within the zone. Where the bolus does not completely fill the circuit an additional gaseous or immiscible liquid spacer such as an oil can help maintain the PCR reaction mix as a discrete bolus.
Thcrcfore, according to said frirther embodiment of thc present invention thc apparatus comprises: (a) a means for receiving a bolus of reaction mix for PCR; (b) a series of zones along the path of the circuit of with defined temperatures which may be different or similar to other zones along the path; (c) a means of circulating the bolus of reaction mix through the temperature zones; and (d) a means of retrieval of amplification products at the conclusion of the PCR.
Claims (24)
- <claim-text>Claims: 1. Immunoassay analytical test apparatus, which apparatus comprises: a. a zone for receiving a sample containing an analyte; b. a zone for receiving a mobile phase, which zone may be the same as the sample receiving zone, or different thereto; e. a zone for receiving immunoreactants which zone may be the same as the sample receiving zone, mobile phase receiving zone or different thereto; d. detection means for permitting detection of said analyte by immunoreaetion; and e. a means for propelling repeated circulation of immunoadsorbent and or immunoreactants.</claim-text> <claim-text>2. Apparatus according to claimi, wherein said immunoadsorbent is circulated through a mobile phase containing immunoreactants.</claim-text> <claim-text>3. Apparatus according to claimi, wherein said immunoadsorbent is able to rotate about an axis.</claim-text> <claim-text>4. Apparatus according to claim!, wherein said immunoadsorbent has surface modifications to enhance physical interaction with the said mobile phase.</claim-text> <claim-text>5. Apparatus according to claim 1, wherein said mobile phase containing immunoreactants is circulated over a stationary immunoadsorbent.</claim-text> <claim-text>6. Apparatus according to claim 1, wherein said mobile phase containing immunoreactants and immunoadsorbent are both moving in opposing directions.</claim-text> <claim-text>7. Apparatus according to claim 1, wherein said mobile phase containing immunoreactants and immunoadsorbent are both moving in the same directions.</claim-text> <claim-text>8. Apparatus according to claim 1, wherein said mobile phase containing immunorcactants and immunoadsorbent are both moving in random directions.</claim-text> <claim-text>9. PCR apparatus, which apparatus comprises: (a) a means for receiving a reaction mix for PCR; (b) a series of zones along the path of the circuit of with defined temperatures which may be different or similar to other zones along the path; (c) a means of moving the reaction mix through the temperature zones; and (d) a means of retrieval of amplification products at the conclusion of thePCR</claim-text> <claim-text>10. Apparatus according to any preceding claim, wherein said movement is due toapplication of a magnetic field.</claim-text> <claim-text>11. Apparatus according to any preceding claim, wherein said movement is due to the action of a pump.</claim-text> <claim-text>12. Apparatus according to any preceding claim, wherein said movement is due to the action of a turbine.</claim-text> <claim-text>13. Apparatus according to any preceding claim, wherein said movement is due to the effect of gravity.</claim-text> <claim-text>14. Apparatus according to any preceding claim, wherein said movement is due to an electric motor.</claim-text> <claim-text>15. Apparatus according to any preceding claim, wherein said movement is due to friction power.</claim-text> <claim-text>16. Apparatus according to any preceding claim, wherein said movement is due to the release of potential energy.</claim-text> <claim-text>17. Apparatus according to any preceding claim, wherein said movement is due to the effect of a change in temperature.</claim-text> <claim-text>18. Apparatus according to any preceding claim, wherein said movement is due to the effect of a change in pressure.</claim-text> <claim-text>19. Apparatus according to any preceding claim, wherein said movement is due to the effect of chemical reactions.</claim-text> <claim-text>20. Apparatus according to any preceding claim, wherein said movement is due to the effect of an electric current.</claim-text> <claim-text>21. Apparatus according to any preceding claim, wherein said movement is due to the flow of liquid.</claim-text> <claim-text>22. Apparatus according to any preceding claim, wherein mixing action is enhanced by the inclusion of baffles.</claim-text> <claim-text>23. Apparatus according to any preceding claim for monitoring allergen specific IgE.</claim-text> <claim-text>24. Apparatus according to any preceding claim, wherein blood samples are separated by a filter before reaching the immunoadsorbent.</claim-text> <claim-text>25. Apparatus according to any preceding claim where the immunoreactants are released from a dried form during the immunoassay procedure by the application of sample.</claim-text> <claim-text>26. Apparatus according to any preceding claim where the immunoreaetants are released from a dried form during the immunoassay procedure by the application of sample containing the analyte.</claim-text> <claim-text>27. Apparatus according to any preceding claim where the immunoreactants are released fix,m a dried form during the immunoassay procedure by the application of a diluent.</claim-text> <claim-text>28. Apparatus according to any preceding claim, wherein analyte and immunoreactants are in contact with the immunoadsorbent simultaneously.</claim-text> <claim-text>29. Apparatus according to any preceding claim, wherein analyte and immunorcactants are in contact with the immunoadsorbcnt sequentially.</claim-text> <claim-text>30. Apparatus according to any preceding claim where part of the dcvicc is disposable.</claim-text> <claim-text>31. Apparatus according to any preceding claim, wherein said immunoadsotbcnt and immunoreactants are exchanged tbr reagents fbr the detection of nucleic acids.</claim-text> <claim-text>32. Apparatus according to any preceding claim, wherein said immunoadsorbent is comprised of one or more organisms or parts thereof.</claim-text> <claim-text>33. Apparatus according to any preceding claim, wherein said immunoreactants is comprised of one or more organisms or parts thereof.</claim-text> <claim-text>34. Apparatus according to any preceding claim, wherein said immunoadsorbent and immunoreactants are comprised of one or more organisms or parts thereof.</claim-text> <claim-text>35. Apparatus according to any preceding claim where one or more said components form part of an electronic chip.</claim-text> <claim-text>36. Apparatus according to any preceding claim, wherein said immunoadsorbent and or imniunoreactants are put in motion by the effects of sub-atomic particles.</claim-text> <claim-text>37. Apparatus according to any preceding claim, wherein said immunoadsorbent and or immunoreactants arc put in motion by the effects of electromagnetic radiation.</claim-text> <claim-text>38. Apparatus according to any preceding claim, wherein said immunoadsorbent and or immunoreactants are put in motion by the effects of sound waves.</claim-text> <claim-text>39. Apparatus according to any preceding claim, wherein said immunoadsorbent and or immunoreactants are put in motion by the effects of gravitational fields.</claim-text> <claim-text>40. Apparatus according to any preceding claim, wherein said immunoadsorbent and or immunorcactants are put in motion by the effects of thcrmal cncrgy.</claim-text> <claim-text>41. An analytical test method or PCR method utilising apparatus according to any preceding claim.Amendments to the claims have been filed as follows Claims: I. An analytical test apparatus, which apparatus comprises: a. a zone for receiving a sample containing an analyte; Ii a zone for receiving a mobile phase, which zone may be the same as the sample receiving zone, or different thereto; c. a zone for receiving reactants which zone may be the same as the sample receiving zone, mobile phase receiving zone or different thereto; d. a detection means for permitting detection of said analyte; and e. a means for rapidly propelling repeated circulation of reactants around a continuous track circuit by the action of an external rotating magnetic field on one or more inert magnetic beads within said continuous track circuit.
- 2. Apparatus according to claim 1, wherein said inert magnetic beads have a corethat is attracted by a magnetic field.
- 3. Apparatus according to claim 1, wherein said inert magnetic beads have a core that is itself a magnet.
- 4. Apparatus according to any preceding claim, wherein the temperatures at discreet locations along the said continuous track circuit arc controlled.
- 5. Apparatus according to any preceding claim that contains an inimunoadsorbent.
- 6. Apparatus according to any preceding claim, wherein said rotating magnetic field forces said inert magnetic beads in motion around a continuous track circuit which in turn forces liquid reactants around said circuit.
- 7. Apparatus according to any preceding claim, wherein said rotating magnetic field forces said inert magnetic beads in motion around a continuous track circuit which in turn pushes one or more non-magnetic immunoadsorbent beads around said circuit.
- 8. Apparatus according to any preceding claim, wherein said reactants comprise of one or more immunoadsorbent beads which are circulated through a mobile phase containing immunoreactants and analyte.
- 9. Apparatus according to any preceding claim, wherein said reactants comprise of inmiunoadsorbent beads separated by inert non magnetic beads.
- 10. Apparatus according to any preceding claim, wherein said i.mmunoadsorbent is able to rotate about an axis.
- 11. Apparatus according to any preceding claim, wherein said mobile phase containing said immunoreactants and analytc is circulated over stationary said inimunoadsorbent. -
- 12. Apparatus according to any preceding claim, wherôin said mobile phase containing said hmnunoreactants and said inununoadsoibent are both moving in relatively opposing directions.
- 13. Apparatus according to any preceding claim, wherein said immunoadsorbent has surface modifications to enhance physical interaction with the said mobile phase.
- 14. Apparatus according to any preceding claim, wherein mixing action is enhanced by the inclusion of baffles.
- 15. Apparatus according to any preceding claim for monitoring allergen specific IgE.
- 16. Apparatus according to any preceding claim, wherein plasma is separated from a blood samples by an integrated filter before reaching said immunoadsorbent.
- 17. Apparatus according to any preceding claim, wherein analyte and said immunoreactants are in contact with said immunoadsorbent simultaneously.
- 18. Apparatus according to any preceding claim, wherein analyte and said irnmunoreactants are in contact with said immunoadsorbent sequentially.
- 19. Apparatus according to any preceding claim wherein pail of the device is disposable.
- 20. Apparatus according to any preceding claim, wherein said immunoadsorbcnt is comprised of one or more organisms or parts thereof
- 21. Apparatus according to any preceding claim, wherein said immunoreactants are comprised of one or more organisms or parts thereof.
- 22. Apparatus according to any preceding claim, wherein said immunoadsorbent and said iminunoreactants are comprised of one or more organisms or parts thereoL
- 23. Apparatus according to claim 1, wherein said reactants are reagents for the detection of nucleic acids.
- 24. An analytical test device utilising apparatus according to any single or combination of preceding claims..</claim-text>
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1117736.7A GB2495536A (en) | 2011-10-14 | 2011-10-14 | Bioanalytical device with mixing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1117736.7A GB2495536A (en) | 2011-10-14 | 2011-10-14 | Bioanalytical device with mixing device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB201117736D0 GB201117736D0 (en) | 2011-11-23 |
| GB2495536A true GB2495536A (en) | 2013-04-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB1117736.7A Withdrawn GB2495536A (en) | 2011-10-14 | 2011-10-14 | Bioanalytical device with mixing device |
Country Status (1)
| Country | Link |
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| GB (1) | GB2495536A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002040874A1 (en) * | 2000-11-16 | 2002-05-23 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| EP1249703A2 (en) * | 2001-04-10 | 2002-10-16 | Hitachi, Ltd. | Apparatus and method for carrying out immunoassays |
| WO2003015923A1 (en) * | 2001-08-20 | 2003-02-27 | Biomicro Systems, Inc. | Fluid mixing in low aspect ratio chambers |
| US20060133954A1 (en) * | 2004-12-21 | 2006-06-22 | Instrumentation Laboratory Company | Resuspension of magnetizable particles |
| WO2008007270A2 (en) * | 2006-06-21 | 2008-01-17 | Spinomix S.A. | A method for manipulating magnetic particles in a liquid medium |
| WO2010057318A1 (en) * | 2008-11-24 | 2010-05-27 | Early Warning Inc. | Devices and methods for providing concentrated biomolecule condensates to biosensing devices |
| US20100189604A1 (en) * | 2008-01-14 | 2010-07-29 | Joan Francesc Guasch | Device For Distributing Particles in a Fluid and Methods Thereof |
| US20110076777A1 (en) * | 2008-07-31 | 2011-03-31 | Adarsh Sandhu | Reaction apparatus and process |
-
2011
- 2011-10-14 GB GB1117736.7A patent/GB2495536A/en not_active Withdrawn
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002040874A1 (en) * | 2000-11-16 | 2002-05-23 | California Institute Of Technology | Apparatus and methods for conducting assays and high throughput screening |
| EP1249703A2 (en) * | 2001-04-10 | 2002-10-16 | Hitachi, Ltd. | Apparatus and method for carrying out immunoassays |
| WO2003015923A1 (en) * | 2001-08-20 | 2003-02-27 | Biomicro Systems, Inc. | Fluid mixing in low aspect ratio chambers |
| US20060133954A1 (en) * | 2004-12-21 | 2006-06-22 | Instrumentation Laboratory Company | Resuspension of magnetizable particles |
| WO2008007270A2 (en) * | 2006-06-21 | 2008-01-17 | Spinomix S.A. | A method for manipulating magnetic particles in a liquid medium |
| US20100189604A1 (en) * | 2008-01-14 | 2010-07-29 | Joan Francesc Guasch | Device For Distributing Particles in a Fluid and Methods Thereof |
| US20110076777A1 (en) * | 2008-07-31 | 2011-03-31 | Adarsh Sandhu | Reaction apparatus and process |
| WO2010057318A1 (en) * | 2008-11-24 | 2010-05-27 | Early Warning Inc. | Devices and methods for providing concentrated biomolecule condensates to biosensing devices |
Also Published As
| Publication number | Publication date |
|---|---|
| GB201117736D0 (en) | 2011-11-23 |
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