[go: up one dir, main page]

GB2452201A - In vitro protein expression process comprising Ck fusion molecules - Google Patents

In vitro protein expression process comprising Ck fusion molecules Download PDF

Info

Publication number
GB2452201A
GB2452201A GB0822625A GB0822625A GB2452201A GB 2452201 A GB2452201 A GB 2452201A GB 0822625 A GB0822625 A GB 0822625A GB 0822625 A GB0822625 A GB 0822625A GB 2452201 A GB2452201 A GB 2452201A
Authority
GB
United Kingdom
Prior art keywords
protein expression
vitro protein
expression process
fusion molecules
gene encoding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
GB0822625A
Other versions
GB0822625D0 (en
Inventor
Mingyue He
Michael John Taussig
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Babraham Institute
Original Assignee
Babraham Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Babraham Institute filed Critical Babraham Institute
Publication of GB0822625D0 publication Critical patent/GB0822625D0/en
Publication of GB2452201A publication Critical patent/GB2452201A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3007—Carcino-embryonic Antigens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/34—Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to an in vitro protein expression process comprising preparation of a nucleic acid molecule which comprises a fusion of a gene encoding a target protein and a gene encoding an immunoglobulin K light chain constant domain (CK) followed by cell-free protein expression. The invention also relates to proteins expressed by said process and to a composition and kit for performing said in vitro protein expression process.
GB0822625A 2006-06-21 2007-06-21 In vitro protein expression process comprising Ck fusion molecules Withdrawn GB2452201A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0612223.8A GB0612223D0 (en) 2006-06-21 2006-06-21 Ck fusion molecules
PCT/GB2007/002309 WO2007148092A2 (en) 2006-06-21 2007-06-21 In vitro protein expression process comprising cκ fusion molecules

Publications (2)

Publication Number Publication Date
GB0822625D0 GB0822625D0 (en) 2009-01-21
GB2452201A true GB2452201A (en) 2009-02-25

Family

ID=36803566

Family Applications (2)

Application Number Title Priority Date Filing Date
GBGB0612223.8A Ceased GB0612223D0 (en) 2006-06-21 2006-06-21 Ck fusion molecules
GB0822625A Withdrawn GB2452201A (en) 2006-06-21 2007-06-21 In vitro protein expression process comprising Ck fusion molecules

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GBGB0612223.8A Ceased GB0612223D0 (en) 2006-06-21 2006-06-21 Ck fusion molecules

Country Status (3)

Country Link
EP (1) EP2029764A2 (en)
GB (2) GB0612223D0 (en)
WO (1) WO2007148092A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9937239B2 (en) 2014-05-21 2018-04-10 The Johns Hopkins University Preservation and reconstitution of cell-free protein expression systems
WO2025252840A1 (en) * 2024-06-04 2025-12-11 Technische Universitaet Muenchen, In Vertretung Des Freistaates Bayern Half-life modulation by a tag comprising at least one constant domain of an immunoglobulin light chain

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0505908A1 (en) * 1991-03-27 1992-09-30 F. Hoffmann-La Roche Ag Chimaeric polypeptides
WO2005087810A2 (en) * 2004-03-08 2005-09-22 Zymogenetics, Inc. Dimeric fusion proteins and materials and methods for producing them

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0505908A1 (en) * 1991-03-27 1992-09-30 F. Hoffmann-La Roche Ag Chimaeric polypeptides
WO2005087810A2 (en) * 2004-03-08 2005-09-22 Zymogenetics, Inc. Dimeric fusion proteins and materials and methods for producing them

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Anal. Biochem., Vol.351, 2006, Son Jeong-Mi, et al., "Enhancing the efficiency...", pp.187-192 *
Biotechnol. Bioengineer., Vol.89, 2005, Yang Junhao, et al., "Rapid expression of...", pp.503-511 *
J. Biotechnol., Vol.115, 2005, Chen Haiqin, et al., "Efficient production of a soluble...", pp.307-315 *
Nucl. Acids Res., Vol.25, 1997, He, et al., "Antibody-ribosome-mRNA...", pp.5132-5134 *
Nucl. Acids Res., VOl.29, 2001, He, M. et al., "Single step generation of...", pp.E73-E73 *
P.N.A.S., Vol.74, 1977, Burstein, Y. et al., "Independent expression of...", pp.3157-3161 *
Protein Sci., Vol.15, 2006, Palmer, E. et al., "Enhanced cell-free protein expression..", pp.2842-2846 *

Also Published As

Publication number Publication date
WO2007148092A8 (en) 2009-07-16
GB0822625D0 (en) 2009-01-21
WO2007148092A3 (en) 2008-02-07
WO2007148092A2 (en) 2007-12-27
EP2029764A2 (en) 2009-03-04
GB0612223D0 (en) 2006-08-02

Similar Documents

Publication Publication Date Title
AR127516A2 (en) Fc VARIANTS AND METHODS FOR ITS PRODUCTION
DK1716178T3 (en) Less immunogenic binding molecules
WO2007130453A3 (en) Non-natural amino acid substituted polypeptides
UA102503C2 (en) Interleukin-13 binding proteins
EA201100516A1 (en) FGF21 MUTANTS AND THEIR APPLICATION
WO2008098100A3 (en) Detection of molecule proximity
EA200970477A1 (en) HUMAN MONOCLONAL ANTIBODIES TO BTLA AND METHODS OF APPLICATION
WO2008140582A3 (en) Production of anti-microbial peptides
WO2009105670A3 (en) Substrates for multiplexed assays and uses thereof
MX346731B (en) Production of heteromultimeric proteins.
MX2009002987A (en) Genetically programmed expression of selectively sulfated proteins in eubacteria.
CL2009000459A1 (en) Antibody binds to rgm a protein; nucleic acid that encodes it; vector; host cell; Method of production; pharmaceutical composition comprising it; antibody construct comprising said protein; antibody conjugate comprising the construct; use.
BRPI0610197B8 (en) sclerostin binding antibody, process for its production, pharmaceutical composition comprising said antibody and cloning or expression vector
WO2009026393A3 (en) Amino acid substituted molecules
MX2007003142A (en) In vivo incorporation of alkynyl amino acids into proteins in eubacteria.
AU2009209251A8 (en) Stabilized Angiopoietin-2 antibodies and uses thereof
WO2009088924A3 (en) Methods and materials for targeted affinity enhancement
UA103176C2 (en) Plant with increased yield-related traits and method for its production
ATE483018T1 (en) NEW FLUORESCENT PROTEINS AND METHODS OF USE THEREOF
MX2009012358A (en) Optical imaging agents.
UA101142C2 (en) Method for antibody designing
EA200801174A1 (en) GLP-2 MIMETIC ANTIBODIES, POLYPEPTIDES, COMPOSITIONS, METHODS AND APPLICATIONS
DK1576157T3 (en) Fluorescent proteins from Copepode species and methods for using the same
WO2007089923A3 (en) Modifications of cst-ii for increased protein expression
GB2452201A (en) In vitro protein expression process comprising Ck fusion molecules

Legal Events

Date Code Title Description
WAP Application withdrawn, taken to be withdrawn or refused ** after publication under section 16(1)