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GB2335038A - Double label immunoassay - Google Patents

Double label immunoassay

Info

Publication number
GB2335038A
GB2335038A GB9908060A GB9908060A GB2335038A GB 2335038 A GB2335038 A GB 2335038A GB 9908060 A GB9908060 A GB 9908060A GB 9908060 A GB9908060 A GB 9908060A GB 2335038 A GB2335038 A GB 2335038A
Authority
GB
United Kingdom
Prior art keywords
component
binding interaction
label
analyte
double label
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB9908060A
Other versions
GB9908060D0 (en
GB2335038B (en
Inventor
David Macallan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xenova Ltd
Original Assignee
Xenova Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9621256.8A external-priority patent/GB9621256D0/en
Application filed by Xenova Ltd filed Critical Xenova Ltd
Priority to GB9908060A priority Critical patent/GB2335038B/en
Publication of GB9908060D0 publication Critical patent/GB9908060D0/en
Publication of GB2335038A publication Critical patent/GB2335038A/en
Application granted granted Critical
Publication of GB2335038B publication Critical patent/GB2335038B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A solid phase assay comprising: (a) providing a solid support on which is immobilised a first component of a binding interaction between the first component and a second component, the first component having a first label which is non-radioactive; (b) contacting the said solid support either (i) with a putative inhibitor of the binding interaction and the second component of the binding interaction, the second component having a second label which is non-radioactive and which is distinguishable front the first label, under conditions under which the binding interaction, in the absence of inhibitor would be expected to occur, or (ii) with a specimen to be assayed for a predetermined analyte, which analyte is the second component of the binding interaction, under conditions under which the binding interaction would be expected to occur if the analyte is present in the specimen; and (c) determining whether, or to what extent, the binding interaction has occurred.
GB9908060A 1996-10-11 1997-09-22 Double label immunoassay Expired - Fee Related GB2335038B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB9908060A GB2335038B (en) 1996-10-11 1997-09-22 Double label immunoassay

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB9621256.8A GB9621256D0 (en) 1996-10-11 1996-10-11 Assay
GB9908060A GB2335038B (en) 1996-10-11 1997-09-22 Double label immunoassay
PCT/GB1997/002552 WO1998016833A1 (en) 1996-10-11 1997-09-22 Double label immunoassay

Publications (3)

Publication Number Publication Date
GB9908060D0 GB9908060D0 (en) 1999-06-02
GB2335038A true GB2335038A (en) 1999-09-08
GB2335038B GB2335038B (en) 2001-03-07

Family

ID=26310217

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9908060A Expired - Fee Related GB2335038B (en) 1996-10-11 1997-09-22 Double label immunoassay

Country Status (1)

Country Link
GB (1) GB2335038B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0093613A1 (en) * 1982-05-04 1983-11-09 Syva Company Simultaneous calibration heterogeneous immunoassay method, apparatus and diagnostic kit
EP0271974A1 (en) * 1986-08-06 1988-06-22 Multilyte Limited Determination of analyte concentration using two labelling markers
WO1991000519A1 (en) * 1989-06-26 1991-01-10 Triton Diagnostics, Inc. Vitamin b12 assay
JPH0472564A (en) * 1990-07-13 1992-03-06 Mitsubishi Kasei Corp immunoassay

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0093613A1 (en) * 1982-05-04 1983-11-09 Syva Company Simultaneous calibration heterogeneous immunoassay method, apparatus and diagnostic kit
EP0271974A1 (en) * 1986-08-06 1988-06-22 Multilyte Limited Determination of analyte concentration using two labelling markers
WO1991000519A1 (en) * 1989-06-26 1991-01-10 Triton Diagnostics, Inc. Vitamin b12 assay
JPH0472564A (en) * 1990-07-13 1992-03-06 Mitsubishi Kasei Corp immunoassay

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Pats Abs of JP vol. 016, no.276 P-1374, 19 6.1992 & JP 04072564 A MITSUBISHI KASEI CORP 6. 3.1992 *

Also Published As

Publication number Publication date
GB9908060D0 (en) 1999-06-02
GB2335038B (en) 2001-03-07

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Legal Events

Date Code Title Description
732E Amendments to the register in respect of changes of name or changes affecting rights (sect. 32/1977)
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20020922