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GB2393725A - Methods for identifying substances interacting with pleckstrin homologydomains, and proteins containing mutated pleckstrin homology domains - Google Patents

Methods for identifying substances interacting with pleckstrin homologydomains, and proteins containing mutated pleckstrin homology domains Download PDF

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Publication number
GB2393725A
GB2393725A GB0402001A GB0402001A GB2393725A GB 2393725 A GB2393725 A GB 2393725A GB 0402001 A GB0402001 A GB 0402001A GB 0402001 A GB0402001 A GB 0402001A GB 2393725 A GB2393725 A GB 2393725A
Authority
GB
United Kingdom
Prior art keywords
compound
pleckstrin
domain
tapp
predicted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB0402001A
Other versions
GB2393725B (en
GB0402001D0 (en
Inventor
Dario Alessi
Aalten Daan Van
Christine Thomas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Dundee
Original Assignee
University of Dundee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Dundee filed Critical University of Dundee
Publication of GB0402001D0 publication Critical patent/GB0402001D0/en
Publication of GB2393725A publication Critical patent/GB2393725A/en
Application granted granted Critical
Publication of GB2393725B publication Critical patent/GB2393725B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method for selecting or designing a compound for modulating the activity of Tandem PH domain containing Protein (TAPP), the method comprising the step of using molecular modelling means to select or design a compound that is predicted to interact with the phosphoinositide binding domain of TAPP, wherein a three-dimensional structure of at least a part of the phos-. phoinoisitide binding domain of the TAPP is compared with a three-dimensional structure of a compound, and a compound that is predicted to interact with the said phosphoinositide binding domain is selected. A method of selecting of designing a compound that modulates the activity of a PH domain-containing polypeptide, the method comprising the step of using molecular modelling means to select or design a compound that is predicted to interact with a PH domain, wherein a three-dimensional structure of a polycarboxylate, for example citrate, is compared with a three-dimensional structure of a compound, and a compound that is predicted on the basis of the structure comparison to interact with the PH domain is selected. Residues of the phosphoinositide binding site of TAPP and DAPP that are involved in determining their phosphoinositide binding specificity are identified. Polypeptides mutated at these residues are useful, for example in compound screening assays.

Description

GB 2393725 A continuation (74) Agent and/or Address for Service: (58)
Field of Search by ISA:
Eric Potter Clarkson INT CL7 C07K Park View House, 58 The Ropewalk, Other: EPO-INTERNAL, BIOSIS, WPI DATA, NOTTINGHAM, NG1 5DD, United Kingdom EMBASE, SEQUENCE SEARCH
GB0402001A 2001-07-31 2002-07-16 Methods for identifying substances interacting with pleckstrin homologydomains, and proteins containing mutated pleckstrin homology domains Expired - Fee Related GB2393725B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0118611A GB0118611D0 (en) 2001-07-31 2001-07-31 Methods
PCT/GB2002/003262 WO2003011901A1 (en) 2001-07-31 2002-07-16 Methods for identifying substances interacting with pleckstrin homology domains, and proteins containing mutated pleckstrin homology domains

Publications (3)

Publication Number Publication Date
GB0402001D0 GB0402001D0 (en) 2004-03-03
GB2393725A true GB2393725A (en) 2004-04-07
GB2393725B GB2393725B (en) 2005-08-31

Family

ID=9919508

Family Applications (2)

Application Number Title Priority Date Filing Date
GB0118611A Ceased GB0118611D0 (en) 2001-07-31 2001-07-31 Methods
GB0402001A Expired - Fee Related GB2393725B (en) 2001-07-31 2002-07-16 Methods for identifying substances interacting with pleckstrin homologydomains, and proteins containing mutated pleckstrin homology domains

Family Applications Before (1)

Application Number Title Priority Date Filing Date
GB0118611A Ceased GB0118611D0 (en) 2001-07-31 2001-07-31 Methods

Country Status (2)

Country Link
GB (2) GB0118611D0 (en)
WO (1) WO2003011901A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040253656A1 (en) * 2003-06-11 2004-12-16 David Williams Method for measuring inositol triphosphate
GB0322689D0 (en) * 2003-09-27 2003-10-29 Medical Res Council Methods

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Biochem J; Vol 351, pp 19-31 (2000). Dowler et al. "Identification of pleckstrin-homology-domain- containing proteins..." *
Biochem J; Vol 358, pp 287-294 (2001). Thomas et al. "Crystal structure of the phosphatidylinositol 3, 4-bisphosphate-binding domain...." *
Curr Opin Cell Biol; Vol 11, pp 219-225 (1999). Leevers et al. "Signalling throh phosphoinositide 3-kinases...." *
Mol Cell; Vol 6, pp 373-384 (2000). Ferguson et al. "Structural basis for discrimination of 3-phosphoinositides..." *
Mol Cell; Vol 6, pp 385-394 (2000). Lietzke et al. "Structral basis of 3-phosphoinositide recognition..." *

Also Published As

Publication number Publication date
WO2003011901A1 (en) 2003-02-13
GB2393725B (en) 2005-08-31
GB0118611D0 (en) 2001-09-19
GB0402001D0 (en) 2004-03-03

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20110716