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GB201716590D0 - Thermostable cas9 nucleases with reduced off-target activity - Google Patents

Thermostable cas9 nucleases with reduced off-target activity

Info

Publication number
GB201716590D0
GB201716590D0 GBGB1716590.3A GB201716590A GB201716590D0 GB 201716590 D0 GB201716590 D0 GB 201716590D0 GB 201716590 A GB201716590 A GB 201716590A GB 201716590 D0 GB201716590 D0 GB 201716590D0
Authority
GB
United Kingdom
Prior art keywords
reduced
target activity
cas9 nucleases
thermostable cas9
thermostable
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
GBGB1716590.3A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wageningen Universiteit
Original Assignee
Wageningen Universiteit
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wageningen Universiteit filed Critical Wageningen Universiteit
Priority to GBGB1716590.3A priority Critical patent/GB201716590D0/en
Publication of GB201716590D0 publication Critical patent/GB201716590D0/en
Priority to PCT/EP2018/076480 priority patent/WO2019072596A1/en
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
GBGB1716590.3A 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity Ceased GB201716590D0 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB1716590.3A GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity
PCT/EP2018/076480 WO2019072596A1 (en) 2017-10-10 2018-09-28 Thermostable cas9 nucleases with reduced off-target activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB1716590.3A GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity

Publications (1)

Publication Number Publication Date
GB201716590D0 true GB201716590D0 (en) 2017-11-22

Family

ID=60326828

Family Applications (1)

Application Number Title Priority Date Filing Date
GBGB1716590.3A Ceased GB201716590D0 (en) 2017-10-10 2017-10-10 Thermostable cas9 nucleases with reduced off-target activity

Country Status (2)

Country Link
GB (1) GB201716590D0 (en)
WO (1) WO2019072596A1 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015330699B2 (en) 2014-10-10 2021-12-02 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
WO2018119010A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Assessing nuclease cleavage
US12110545B2 (en) 2017-01-06 2024-10-08 Editas Medicine, Inc. Methods of assessing nuclease cleavage
EP3615672A1 (en) 2017-04-28 2020-03-04 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
KR102746733B1 (en) 2017-06-09 2024-12-24 에디타스 메디신, 인코포레이티드 Engineered CAS9 nuclease
EP3652312A1 (en) 2017-07-14 2020-05-20 Editas Medicine, Inc. Systems and methods for targeted integration and genome editing and detection thereof using integrated priming sites
AU2019291918B2 (en) 2018-06-29 2025-06-12 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
JP7448120B2 (en) * 2019-11-14 2024-03-12 国立研究開発法人農業・食品産業技術総合研究機構 Method for introducing genome editing enzymes into plant cells using plasma

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201510296D0 (en) * 2015-06-12 2015-07-29 Univ Wageningen Thermostable CAS9 nucleases
US11242513B2 (en) * 2016-12-14 2022-02-08 Wageningen Universiteit Thermostable Cas9 nucleases
WO2018109101A1 (en) * 2016-12-14 2018-06-21 Wageningen Universiteit Thermostable cas9 nucleases

Also Published As

Publication number Publication date
WO2019072596A1 (en) 2019-04-18

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Legal Events

Date Code Title Description
AT Applications terminated before publication under section 16(1)