GB2007244A - Purifying Polyribosyl Ribitol Phosphate (PRP) and Vaccines containing Immunologically Active PRP - Google Patents
Purifying Polyribosyl Ribitol Phosphate (PRP) and Vaccines containing Immunologically Active PRPInfo
- Publication number
- GB2007244A GB2007244A GB7842230A GB7842230A GB2007244A GB 2007244 A GB2007244 A GB 2007244A GB 7842230 A GB7842230 A GB 7842230A GB 7842230 A GB7842230 A GB 7842230A GB 2007244 A GB2007244 A GB 2007244A
- Authority
- GB
- United Kingdom
- Prior art keywords
- prp
- immunologically active
- vaccines containing
- antibody
- purifying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229960005486 vaccine Drugs 0.000 title abstract 3
- VJDOAZKNBQCAGE-LMVFSUKVSA-N D-ribitol 5-phosphate Chemical compound OC[C@H](O)[C@H](O)[C@H](O)COP(O)(O)=O VJDOAZKNBQCAGE-LMVFSUKVSA-N 0.000 title abstract 2
- 238000000034 method Methods 0.000 abstract 4
- 241000588832 Bordetella pertussis Species 0.000 abstract 2
- 239000000427 antigen Substances 0.000 abstract 2
- 102000036639 antigens Human genes 0.000 abstract 2
- 108091007433 antigens Proteins 0.000 abstract 2
- 238000000746 purification Methods 0.000 abstract 2
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical class [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 abstract 1
- 241000606768 Haemophilus influenzae Species 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 239000003463 adsorbent Substances 0.000 abstract 1
- 230000000844 anti-bacterial effect Effects 0.000 abstract 1
- 238000010923 batch production Methods 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 229910000389 calcium phosphate Inorganic materials 0.000 abstract 1
- 239000001506 calcium phosphate Substances 0.000 abstract 1
- 235000011010 calcium phosphates Nutrition 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 238000005755 formation reaction Methods 0.000 abstract 1
- 229940045808 haemophilus influenzae type b Drugs 0.000 abstract 1
- 229910052588 hydroxylapatite Inorganic materials 0.000 abstract 1
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/099—Bordetella
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
- A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/70—Multivalent vaccine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides methods for the purification of polyribosyl ribitol phosphate (PRP) from Haemophilus influenzae type b cultures, as well as novel forms of immunologically active PRP obtainable by such methods and combined vaccines containing immunologically active PRP and Bordetella pertussis antigens. The purification methods involve removing contaminants to a low level by treating the PRP in the form of a hexadecyltrimethyl ammonium bromide complex with a calcium phosphate adsorbent (hydroxylapatite) in a phosphate buffer, and may be practiced either as a batch process or as a column process. The purified PRP thus obtainable is immunologically active and may be used with B. pertussis antigens to form a combined vaccine which elicits anti-PRP antibody and antipercussis antibody formations in young animals. This sera with anti-PRP antibody exhibits a strong bactericidal activity.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/846,466 US4196192A (en) | 1977-10-28 | 1977-10-28 | Combined Haemophilus influenzae type b and pertussis vaccine |
| US05/846,488 US4220717A (en) | 1977-12-22 | 1977-12-22 | Isolation and purification of polyribosyl ribitol phosphate from Haemophilus influenzae type b. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| GB2007244A true GB2007244A (en) | 1979-05-16 |
| GB2007244B GB2007244B (en) | 1982-03-31 |
Family
ID=27126641
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB7842230A Expired GB2007244B (en) | 1977-10-28 | 1978-10-27 | Purifying polyribosyl ribitol phosphate (prp) and vaccines containing immunoligically active prp |
Country Status (25)
| Country | Link |
|---|---|
| JP (1) | JPS5480408A (en) |
| AR (1) | AR218932A1 (en) |
| AU (1) | AU520902B2 (en) |
| BE (1) | BE871586A (en) |
| CA (1) | CA1117866A (en) |
| CH (1) | CH649781A5 (en) |
| DD (1) | DD140701A5 (en) |
| DE (1) | DE2845745A1 (en) |
| DK (1) | DK154327C (en) |
| ES (1) | ES474611A1 (en) |
| FI (1) | FI63674C (en) |
| FR (1) | FR2407000B1 (en) |
| GB (1) | GB2007244B (en) |
| GR (1) | GR73991B (en) |
| HU (1) | HU178166B (en) |
| IE (1) | IE47474B1 (en) |
| IL (1) | IL55433A (en) |
| IT (1) | IT1107570B (en) |
| NL (1) | NL7810753A (en) |
| NO (1) | NO149611C (en) |
| NZ (1) | NZ188211A (en) |
| PH (1) | PH15882A (en) |
| PL (1) | PL210545A1 (en) |
| SE (1) | SE430753B (en) |
| YU (1) | YU250878A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2121365C1 (en) * | 1992-10-27 | 1998-11-10 | Американ Цианамид Компани | The mixed vaccine, a method of simultaneous mixed vaccination and a method of immunogenicity increase |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE889979A (en) * | 1981-08-14 | 1982-02-15 | Smith Kline Rit | PROCESS FOR THE PREPARATION OF PURIFIED ANTIGENIC CAPSULAR BACTERIAL POLYSACCHARIDES, PRODUCTS OBTAINED AND THEIR USE |
| CA1209036A (en) * | 1982-08-20 | 1986-08-05 | Joseph S.C. Kuo | Combined haemophilus influenzae and diphtheria, pertussis, tetanus vaccine |
| US4744982A (en) * | 1982-08-24 | 1988-05-17 | Hunter Kenneth W | Human monoclonal antibody reactive with polyribosylribitol phosphate |
| DE69113564T2 (en) * | 1990-08-13 | 1996-05-30 | American Cyanamid Co | Bordetella pertussis fiber hemagglutinin as a carrier for conjugated vaccine. |
| HU9400426D0 (en) * | 1991-08-16 | 1994-05-30 | Merck & Co Inc | Process for converting lipid-containing bacterial capsular polysaccharide into lipid-free polysaccharide |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3395219A (en) * | 1964-12-11 | 1968-07-30 | Merck & Co Inc | Process for production of pertussis antigen |
| NO116869B (en) * | 1965-01-19 | 1969-06-02 | Merck & Co Inc | |
| US3465078A (en) * | 1965-10-21 | 1969-09-02 | Sydney Z Spiesel | Method of recovering antigens from bordetella pertussis cells |
| NL174267B (en) * | 1969-05-20 | Roussel Uclaf | IMPROVEMENT OF THE PROCESS FOR THE PREPARATION OF A SOMATIC ANTIGEN ACCORDING TO DUTCH PATENT 169754 AND PROCESS FOR PREPARING A PHARMACEUTICAL PREPARATION. | |
| US3636192A (en) * | 1970-01-13 | 1972-01-18 | Us Army | Meningococcal polysaccharide vaccines |
| FR2253499B1 (en) * | 1973-12-10 | 1977-11-04 | Fabre Sa Pierre | |
| DE2461439C3 (en) * | 1974-12-24 | 1980-03-20 | Behringwerke Ag, 3550 Marburg | Process for the preparation of a protective antigen from Bordetella pertussis and agent containing the same |
| US3978209A (en) * | 1975-03-24 | 1976-08-31 | Merck & Co., Inc. | Endotoxin free meningococcus polysaccharides |
-
1978
- 1978-08-22 NZ NZ188211A patent/NZ188211A/en unknown
- 1978-08-25 IL IL55433A patent/IL55433A/en unknown
- 1978-08-29 CA CA000310218A patent/CA1117866A/en not_active Expired
- 1978-08-30 AR AR273492A patent/AR218932A1/en active
- 1978-08-30 GR GR57127A patent/GR73991B/el unknown
- 1978-10-20 DE DE19782845745 patent/DE2845745A1/en active Granted
- 1978-10-24 IT IT51621/78A patent/IT1107570B/en active
- 1978-10-25 PH PH21738A patent/PH15882A/en unknown
- 1978-10-26 AU AU41081/78A patent/AU520902B2/en not_active Expired
- 1978-10-26 FI FI783269A patent/FI63674C/en not_active IP Right Cessation
- 1978-10-26 FR FR7830517A patent/FR2407000B1/fr not_active Expired
- 1978-10-27 NL NL7810753A patent/NL7810753A/en not_active Application Discontinuation
- 1978-10-27 BE BE191385A patent/BE871586A/en not_active IP Right Cessation
- 1978-10-27 DD DD78208715A patent/DD140701A5/en unknown
- 1978-10-27 DK DK479578A patent/DK154327C/en not_active IP Right Cessation
- 1978-10-27 YU YU02508/78A patent/YU250878A/en unknown
- 1978-10-27 SE SE7811192A patent/SE430753B/en not_active IP Right Cessation
- 1978-10-27 HU HU78AE546A patent/HU178166B/en not_active IP Right Cessation
- 1978-10-27 IE IE2146/78A patent/IE47474B1/en unknown
- 1978-10-27 CH CH11125/78A patent/CH649781A5/en not_active IP Right Cessation
- 1978-10-27 PL PL21054578A patent/PL210545A1/xx unknown
- 1978-10-27 GB GB7842230A patent/GB2007244B/en not_active Expired
- 1978-10-27 NO NO783635A patent/NO149611C/en unknown
- 1978-10-27 ES ES474611A patent/ES474611A1/en not_active Expired
- 1978-10-28 JP JP13310978A patent/JPS5480408A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2121365C1 (en) * | 1992-10-27 | 1998-11-10 | Американ Цианамид Компани | The mixed vaccine, a method of simultaneous mixed vaccination and a method of immunogenicity increase |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE87003T1 (en) | OLIGOSACCHARIDES, IMMUNOGENS AND VACCINES AND METHODS OF MANUFACTURE OF SUCH OLIGOSACCHARIDES, IMMUNOGENS AND VACCINES. | |
| DK475779A (en) | PROCEDURE FOR MANUFACTURING MEMBRANE PROTEINS FROM NEISSERIA MENINGITIDIS AND VACCINE CONTAINING SUCH MEMBRANE PROTEINS | |
| SE8305535D0 (en) | PHARMACEUTICAL PREPARATION WITH ANTINICOTIC EFFECTS AND PROCEDURES FOR PREPARING THEREOF | |
| DE3481514D1 (en) | NEW FRUCTOSYLTSANSFERASE AND METHOD FOR PRODUCING FRUCTOSYL DISACCHARIDES, AND A NEW HALOSACCHAROSE. | |
| GB2007244A (en) | Purifying Polyribosyl Ribitol Phosphate (PRP) and Vaccines containing Immunologically Active PRP | |
| ATE119196T1 (en) | METHOD FOR PRODUCING A FACTOR-VIIA ENRICHED FRACTION AND USE THEREOF AS A MEDICINAL PRODUCT. | |
| US3957582A (en) | Purification of urokinase | |
| DE3787887D1 (en) | Process for growing Bordetella pertussis, a pertussis toxoid and a pertussis vaccine. | |
| DK199588D0 (en) | VACCINE | |
| GR3018440T3 (en) | Novel vaccine and method therefor. | |
| FR2597344B1 (en) | IMPROVEMENT IN THE PROCESS OF PURIFYING PROTEIN ANTIGENS FROM BACTERIA BELONGING TO THE GENUS BORDETELLA, WITH A VIEW TO OBTAINING A CELLULAR VACCINE. | |
| BG39465A3 (en) | METHOD FOR PURIFICATION OF CHEMICAL GYPSUM | |
| ATE420951T1 (en) | PROTECTIVE EPITOPES OF ADENYLATE CYCLASE-HEMOLYSIN (AC-HLY) AND THEIR USE FOR TREATING OR PREVENTING BORDETELLA INFECTIONS | |
| PT78043A (en) | Process for the preparation of antisebhorreic sinalised compositions conaining a silicagel | |
| DE69109314D1 (en) | Human monoclonal antibody and pharmaceutical composition containing it for the treatment of Pseudomonas infections. | |
| DE3777743D1 (en) | METHOD FOR PRODUCING 1,4-DIAZABICYCLO (2,2,2) -OCTANES. | |
| ATE110279T1 (en) | PROCESS FOR THE PRODUCTION OF A PERTUSSIS TOXIN TOXOIDE. | |
| ATE21033T1 (en) | PROCESS FOR THE PRODUCTION OF AN IMMUNOGEN FROM PASTEURELLA MULTOCIDA. | |
| SE8003761L (en) | PROCEDURE FOR PREPARING A MODIFIED CRYSTAL FORM OF FLUNISOLIDE | |
| SU551894A2 (en) | METHOD OF CLEANING VIRUS-CONTAINING SUSPENSION | |
| SU1563237A1 (en) | METHOD FOR INACTIVATION OF PIGS CALICIVIRUS |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PCNP | Patent ceased through non-payment of renewal fee |