GB1432039A - Method of treating microorganisms - Google Patents
Method of treating microorganismsInfo
- Publication number
- GB1432039A GB1432039A GB5176473A GB5176473A GB1432039A GB 1432039 A GB1432039 A GB 1432039A GB 5176473 A GB5176473 A GB 5176473A GB 5176473 A GB5176473 A GB 5176473A GB 1432039 A GB1432039 A GB 1432039A
- Authority
- GB
- United Kingdom
- Prior art keywords
- treatment
- slurry
- rupturing
- cells
- mins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 244000005700 microbiome Species 0.000 title abstract 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 6
- 210000004027 cell Anatomy 0.000 abstract 5
- 239000002002 slurry Substances 0.000 abstract 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 abstract 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 abstract 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 abstract 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 abstract 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 2
- 239000003513 alkali Substances 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract 2
- 239000004576 sand Substances 0.000 abstract 2
- 239000002699 waste material Substances 0.000 abstract 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 abstract 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 abstract 1
- 241001495180 Arthrospira Species 0.000 abstract 1
- 240000002900 Arthrospira platensis Species 0.000 abstract 1
- 235000016425 Arthrospira platensis Nutrition 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- 241000222178 Candida tropicalis Species 0.000 abstract 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 abstract 1
- 240000009108 Chlorella vulgaris Species 0.000 abstract 1
- 235000007089 Chlorella vulgaris Nutrition 0.000 abstract 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 abstract 1
- 241000186249 Corynebacterium sp. Species 0.000 abstract 1
- 241000250507 Gigaspora candida Species 0.000 abstract 1
- 108010068370 Glutens Proteins 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 abstract 1
- 235000010469 Glycine max Nutrition 0.000 abstract 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 abstract 1
- 235000003534 Saccharomyces carlsbergensis Nutrition 0.000 abstract 1
- 244000253911 Saccharomyces fragilis Species 0.000 abstract 1
- 235000018368 Saccharomyces fragilis Nutrition 0.000 abstract 1
- 241001123227 Saccharomyces pastorianus Species 0.000 abstract 1
- 229920002472 Starch Polymers 0.000 abstract 1
- 241000235015 Yarrowia lipolytica Species 0.000 abstract 1
- 239000002253 acid Substances 0.000 abstract 1
- 238000010306 acid treatment Methods 0.000 abstract 1
- 229910021529 ammonia Inorganic materials 0.000 abstract 1
- 239000011324 bead Substances 0.000 abstract 1
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 239000000919 ceramic Substances 0.000 abstract 1
- 235000013339 cereals Nutrition 0.000 abstract 1
- 235000013312 flour Nutrition 0.000 abstract 1
- 239000011521 glass Substances 0.000 abstract 1
- 235000021312 gluten Nutrition 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 abstract 1
- 230000003834 intracellular effect Effects 0.000 abstract 1
- 239000004310 lactic acid Substances 0.000 abstract 1
- 235000014655 lactic acid Nutrition 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 230000000717 retained effect Effects 0.000 abstract 1
- 229910052708 sodium Inorganic materials 0.000 abstract 1
- 239000011734 sodium Substances 0.000 abstract 1
- 235000010413 sodium alginate Nutrition 0.000 abstract 1
- 229940005550 sodium alginate Drugs 0.000 abstract 1
- 239000000661 sodium alginate Substances 0.000 abstract 1
- 229940080313 sodium starch Drugs 0.000 abstract 1
- 229940082787 spirulina Drugs 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/18—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from yeasts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/22—Working-up of proteins for foodstuffs by texturising
- A23J3/225—Texturised simulated foods with high protein content
- A23J3/227—Meat-like textured foods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1432039 Extracting contents of microorganism cells DAI NIPPON SUGAR MFG CO Ltd 7 Nov 1973 [10 Nov 1972 (2)] 51764/73 Headings A2B and C6F [Also in Division C6] An aqueous slurry of microorganisms containing 2-32% dry weight of living cells is treated with at least one agent to weaken' the cell walls, the agent being an acid, alkali, or hydrophilic organic solvent, for 20 mins-2 hrs at <70‹C for pH 0.5-4.0, for 10 mins- 16 hrs at 25-65‹C for pH 7.0-10.5 or for 4 mins-6 hrs at 0-30‹C for pH 10.5-12.0, and then the cells are mechanically ruptured using a grinder or roll mill. The microorganism may be a yeast (e.g. Candida utilis, Saccharomyces cerevisiae, Saccharomyces fragilis, Saccharomyces carlsbergensis, Candida tropicalis, Candida lipolytica, or Rodotorula glutinis), or a bacterium (e.g. Bacillus subtilis or Corynebacterium sp.) or an alga (e.g. Arthrospira, Spirulina, Scenedestius sp. and Chlorella vulgaris). The alkali for treatment at pH 7.0-10.5 may be ammonia or a hydroxide, carbonate or alkaline lactate, citrate, succinate, or acetate of Na, K, NH 3 , Mg or Ca, and for treatment at pH 10.5-12.9 NaOH or KOH may be used, whilst for acid treatment hydrochloric, phosphoric, acetic, lactic, or citric acid may be used. Treatment at pH 7.5-9.5 may be carried out in the presence of ethyl acetate, toluene, acetone, or an alcohol in an amount of 0.5-3% of the weight of the slurry. Treatment may be effected using acetone, methanol, ethanol, propanol or isopropanol preferably in an amount <2.4 times that of the cell moisture content at <30‹C till the intracellular moisture content is <30% w/w. Preferably the cells are separated from the residual waste liquor before rupturing. Preferred rupturing devices are a ball mill containing glass or ceramic beads, or Zr or Al balls having a size of 0.2-1.5 mm or a sand grinder containing 30-90% v/u sand. The rupturing may be carried out continuously with a slurry input rate of 1.8- 40 times the vessel volume portions. A threeroll, high-speed mill, may be used, the front roll being rotated at 120-350 m/min at 20-40 kg/cm<SP>2</SP> pressure with an input of slurry containing 45-65 wt% moisture. Prior to rupturing the slurry may be mixed with cereal flour, powdered gluten, soya bean protein, sodium alginate or starch. Waste liquor may be retained, adjusted to pH 1-7, and dried as an extract, possibly after treatment at pH 5.0-6.0 with activated carbon.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP11202672A JPS4971186A (en) | 1972-11-10 | 1972-11-10 | |
| JP11202772A JPS5530834B2 (en) | 1972-11-10 | 1972-11-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1432039A true GB1432039A (en) | 1976-04-14 |
Family
ID=26451284
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB5176473A Expired GB1432039A (en) | 1972-11-10 | 1973-11-07 | Method of treating microorganisms |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1432039A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0008728A3 (en) * | 1978-08-26 | 1980-04-16 | Deutschen Hefewerke Gestellschaft Mit Beschrankter Haftung | Yeast autolysate, its preparation and its application in food and fodder |
| EP0173924A3 (en) * | 1984-09-01 | 1988-02-03 | Boehringer Ingelheim International G.M.B.H | Mechanical process for dislodging bacterial cells for the isolation of peptides prepared by recombinant techniques |
| WO1988008881A1 (en) * | 1987-05-11 | 1988-11-17 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
| EP0342892A1 (en) * | 1988-05-17 | 1989-11-23 | Schering Corporation | Extraction of human interleukin-4 from bacteria |
| WO2005016024A1 (en) * | 2003-08-15 | 2005-02-24 | Grain Processing Corporation | Method for dissociation of cells |
| WO2015001261A1 (en) * | 2013-07-04 | 2015-01-08 | Roquette Freres | Optimised method for breaking chlorella walls by mechanical crushing |
| US9386774B2 (en) * | 2014-12-16 | 2016-07-12 | Heliae Development, Llc | Application of mixotrophic chlorella for the improved yield and quality of solanaceae plants |
| US11102985B2 (en) | 2017-11-10 | 2021-08-31 | Heliae Development, Llc | Biomass compositions and methods for making the same |
| CN114249409A (en) * | 2021-11-26 | 2022-03-29 | 河海大学 | Pre-oxidation coupling pressurizing device and method for river and lake blue algae treatment |
-
1973
- 1973-11-07 GB GB5176473A patent/GB1432039A/en not_active Expired
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0008728A3 (en) * | 1978-08-26 | 1980-04-16 | Deutschen Hefewerke Gestellschaft Mit Beschrankter Haftung | Yeast autolysate, its preparation and its application in food and fodder |
| EP0173924A3 (en) * | 1984-09-01 | 1988-02-03 | Boehringer Ingelheim International G.M.B.H | Mechanical process for dislodging bacterial cells for the isolation of peptides prepared by recombinant techniques |
| WO1988008881A1 (en) * | 1987-05-11 | 1988-11-17 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
| EP0291294A1 (en) * | 1987-05-11 | 1988-11-17 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
| US5136024A (en) * | 1987-05-11 | 1992-08-04 | Schering Corporation | Extraction of granulocyte macrophage colony stimulating factor from bacteria |
| EP0342892A1 (en) * | 1988-05-17 | 1989-11-23 | Schering Corporation | Extraction of human interleukin-4 from bacteria |
| WO1989011541A1 (en) * | 1988-05-17 | 1989-11-30 | Schering Corporation | Extraction of human interleukin-4 from bacteria |
| US4958007A (en) * | 1988-05-17 | 1990-09-18 | Schering-Plough Corp. | Extraction of human interleukin-4- from bacteria |
| WO2005016024A1 (en) * | 2003-08-15 | 2005-02-24 | Grain Processing Corporation | Method for dissociation of cells |
| US7425439B2 (en) | 2003-08-15 | 2008-09-16 | Grain Processing Corporation | Method for dissociation of cells |
| WO2015001261A1 (en) * | 2013-07-04 | 2015-01-08 | Roquette Freres | Optimised method for breaking chlorella walls by mechanical crushing |
| FR3008001A1 (en) * | 2013-07-04 | 2015-01-09 | Roquette Freres | OPTIMIZED METHOD OF BREAKING CHLORELLA WALLS BY MECHANICAL MILLING |
| US10465159B2 (en) | 2013-07-04 | 2019-11-05 | Corbion Biotech, Inc. | Optimised method for breaking chlorella walls by mechanical crushing |
| US9386774B2 (en) * | 2014-12-16 | 2016-07-12 | Heliae Development, Llc | Application of mixotrophic chlorella for the improved yield and quality of solanaceae plants |
| US11102985B2 (en) | 2017-11-10 | 2021-08-31 | Heliae Development, Llc | Biomass compositions and methods for making the same |
| CN114249409A (en) * | 2021-11-26 | 2022-03-29 | 河海大学 | Pre-oxidation coupling pressurizing device and method for river and lake blue algae treatment |
Also Published As
| Publication number | Publication date |
|---|---|
| AU6234573A (en) | 1975-05-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Potgieter et al. | Susceptibility and resistance of several fungi to microbial lysis | |
| KR101558430B1 (en) | Mild alkaline pretreatment and simultaneous saccharification and fermentation of lignocellulosic biomass into organic acids | |
| GB1432039A (en) | Method of treating microorganisms | |
| CN1330671C (en) | Chitosan-containing polysaccharide, method for preparing the same and use thereof | |
| Bartley | Changes in the glucans of ripening apples | |
| US8679797B2 (en) | Method for preparing glucan and mannan, glucan preparation and mannan preparation produced thereby and use thereof | |
| Tokunaga et al. | Cyst wall formation and endogenous carbohydrate utilization during synchronous encystment of Phytophthora palmivora zoospores | |
| MY131626A (en) | Processing of yeast refuse and resulting product | |
| Johnson et al. | Simple method for the isolation of astaxanthin from the basidiomycetous yeast Phaffia rhodozyma | |
| JP4270596B2 (en) | Method for producing water-soluble sugars from cereal hulls | |
| GB568508A (en) | Improvements in or relating to the production of citric acid by fermentation | |
| GB1485502A (en) | Process for removal of water-soluble carbohydrates in the production of plant protein products | |
| Knorr et al. | An enzymatic method fob yeast autolysis | |
| CN106495827A (en) | The method that alkali carries and classification enzymolysis joint prepare the organic liquid fertilizer of ocean containing oligosaccharide | |
| US3969189A (en) | Cell wall-lysing complex enzymes and a process for the production thereof | |
| Takaya et al. | Degradation of Starch Granules by Alpha‐Amylase of Streptomyces precox NA‐273 | |
| EP0566347A2 (en) | Use of yeast cell debris products | |
| GB1474313A (en) | Process for preparing a protein concentrate from a microbial cell mass and the protein concentrate thus obtained | |
| US4713334A (en) | Process for the saccharification of celluloses | |
| Reed-Hamer et al. | Effect of complex nitrogen sources on pullulan production relative to carbon source | |
| US3890198A (en) | Cell wall-lysing complex enzymes and a process for the production thereof | |
| Ryan et al. | Study of the effect of β-1, 3-glucanase from Basidiomycete QM 806 on yeast extract production | |
| CZ293310B6 (en) | Method for isolating immunostimulating glucan from oyster cap mushroom | |
| GB1221272A (en) | Process for producing cell-lytic enzymes | |
| Reese | AEROBIC DECOMPOSITION OF CELLULOSE BY MICRO-ORGANISMS AT TEMPERATURES ABOVE 40 DEGREES C |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |