GB1007971A - Procedure for the recovery of vitamin b-12 from rich microorganism cells - Google Patents
Procedure for the recovery of vitamin b-12 from rich microorganism cellsInfo
- Publication number
- GB1007971A GB1007971A GB4430160A GB4430160A GB1007971A GB 1007971 A GB1007971 A GB 1007971A GB 4430160 A GB4430160 A GB 4430160A GB 4430160 A GB4430160 A GB 4430160A GB 1007971 A GB1007971 A GB 1007971A
- Authority
- GB
- United Kingdom
- Prior art keywords
- vitamin
- tannic acid
- precipitate
- suspension
- recovery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 244000005700 microbiome Species 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000011084 recovery Methods 0.000 title 1
- 229940088594 vitamin Drugs 0.000 title 1
- 229930003231 vitamin Natural products 0.000 title 1
- 235000013343 vitamin Nutrition 0.000 title 1
- 239000011782 vitamin Substances 0.000 title 1
- 150000003722 vitamin derivatives Chemical class 0.000 title 1
- 229930003779 Vitamin B12 Natural products 0.000 abstract 4
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 abstract 4
- 239000011715 vitamin B12 Substances 0.000 abstract 4
- 235000019163 vitamin B12 Nutrition 0.000 abstract 4
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 abstract 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- 239000001263 FEMA 3042 Substances 0.000 abstract 3
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 abstract 3
- 239000000725 suspension Substances 0.000 abstract 3
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 abstract 3
- 229940033123 tannic acid Drugs 0.000 abstract 3
- 235000015523 tannic acid Nutrition 0.000 abstract 3
- 229920002258 tannic acid Polymers 0.000 abstract 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 2
- 238000004587 chromatography analysis Methods 0.000 abstract 2
- 238000001914 filtration Methods 0.000 abstract 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000000706 filtrate Substances 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 239000012535 impurity Substances 0.000 abstract 1
- 238000005342 ion exchange Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 239000011780 sodium chloride Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H23/00—Compounds containing boron, silicon or a metal, e.g. chelates or vitamin B12
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/42—Cobalamins, i.e. vitamin B12, LLD factor
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
Vitamin B12 is obtained from an alkaline saline suspension of micro-organism cells obtained during the fomentation of a Vitamin B12 producing micro-organism by heating the suspension, separating the spent cells, treating the resulting aqueous solution with CaO to precipitate impurities, filtering, adding tannic acid to the filtrate to precipitate a Vitamin B12 tannic acid complex and recovering the Vitamin B12 preferably by ion exchange or chromatography. In an example, a salt suspension obtained according to the method of Specification 1,007,972 was heated to 100-120 DEG C. for 10 minutes. The mixture was then extracted with acetone and treated with CaO. After filtering, tannic acid was added to the solution and the resulting precipitate was dissolved in acetic acid and purified by chromatography on alumina.
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4430160A GB1007971A (en) | 1960-12-23 | 1960-12-23 | Procedure for the recovery of vitamin b-12 from rich microorganism cells |
| CH1476561A CH422229A (en) | 1960-12-23 | 1961-12-20 | Process for the isolation and purification of vitamin B12 |
| DE1961P0028466 DE1213844B (en) | 1960-12-23 | 1961-12-22 | Process for obtaining pure vitamin B. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4430160A GB1007971A (en) | 1960-12-23 | 1960-12-23 | Procedure for the recovery of vitamin b-12 from rich microorganism cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1007971A true GB1007971A (en) | 1965-10-22 |
Family
ID=10432660
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4430160A Expired GB1007971A (en) | 1960-12-23 | 1960-12-23 | Procedure for the recovery of vitamin b-12 from rich microorganism cells |
Country Status (3)
| Country | Link |
|---|---|
| CH (1) | CH422229A (en) |
| DE (1) | DE1213844B (en) |
| GB (1) | GB1007971A (en) |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1068429B (en) * | 1959-11-05 | Anrni'.: Societä Farmaceutici Italia, Mailand (Italien) | Process for the microbial production of cobalamines by Nocardia rugosa in the presence of diamines as precursors | |
| DE1073151B (en) * | 1960-01-14 | |||
| DE947193C (en) * | 1949-06-21 | 1956-08-09 | Distillers Co Yeast Ltd | Process for obtaining vitamin B. |
| DE842527C (en) * | 1949-09-09 | 1952-06-26 | Bayer Ag | Process for obtaining vitamin B 12 |
| DE1060549B (en) * | 1957-07-03 | 1959-07-02 | Uclaf Fa | Method of extraction of vitamin B. |
| US2920015A (en) * | 1957-08-27 | 1960-01-05 | Armour & Co | Long-acting vitamin b12 |
-
1960
- 1960-12-23 GB GB4430160A patent/GB1007971A/en not_active Expired
-
1961
- 1961-12-20 CH CH1476561A patent/CH422229A/en unknown
- 1961-12-22 DE DE1961P0028466 patent/DE1213844B/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE1213844B (en) | 1966-04-07 |
| CH422229A (en) | 1966-10-15 |
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