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FR3100820B1 - Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism - Google Patents

Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism Download PDF

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Publication number
FR3100820B1
FR3100820B1 FR1910106A FR1910106A FR3100820B1 FR 3100820 B1 FR3100820 B1 FR 3100820B1 FR 1910106 A FR1910106 A FR 1910106A FR 1910106 A FR1910106 A FR 1910106A FR 3100820 B1 FR3100820 B1 FR 3100820B1
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France
Prior art keywords
processing
large number
samples
cases
process suitable
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FR1910106A
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French (fr)
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FR3100820A1 (en
Inventor
Pascal Herve
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Bionobis SARL
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Bionobis SARL
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Priority to FR1910106A priority Critical patent/FR3100820B1/en
Priority to EP20786024.8A priority patent/EP4028548A1/en
Priority to PCT/FR2020/051568 priority patent/WO2021048504A1/en
Publication of FR3100820A1 publication Critical patent/FR3100820A1/en
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Publication of FR3100820B1 publication Critical patent/FR3100820B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L’invention a trait à un procédé de génotypage permettant de traiter simultanément un grand nombre d’échantillons d’ADN. Plus particulièrement, elle concerne un procédé de génotypage comportant une première étape d’amplification par PCR indexée puis une seconde étape de séquençage à haut débit, ce procédé étant simple, rapide et peu coûteux.The invention relates to a genotyping method for processing a large number of DNA samples simultaneously. More particularly, it relates to a genotyping process comprising a first step of amplification by indexed PCR then a second step of high-throughput sequencing, this process being simple, rapid and inexpensive.

FR1910106A 2019-09-12 2019-09-12 Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism Active FR3100820B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR1910106A FR3100820B1 (en) 2019-09-12 2019-09-12 Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism
EP20786024.8A EP4028548A1 (en) 2019-09-12 2020-09-11 Genotyping method suitable for processing a large number of samples, in particular in cases of high polymorphism
PCT/FR2020/051568 WO2021048504A1 (en) 2019-09-12 2020-09-11 Genotyping method suitable for processing a large number of samples, in particular in cases of high polymorphism

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1910106A FR3100820B1 (en) 2019-09-12 2019-09-12 Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism
FR1910106 2019-09-12

Publications (2)

Publication Number Publication Date
FR3100820A1 FR3100820A1 (en) 2021-03-19
FR3100820B1 true FR3100820B1 (en) 2024-06-28

Family

ID=69190887

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1910106A Active FR3100820B1 (en) 2019-09-12 2019-09-12 Genotyping process suitable for processing a large number of samples, particularly in cases of high polymorphism

Country Status (3)

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EP (1) EP4028548A1 (en)
FR (1) FR3100820B1 (en)
WO (1) WO2021048504A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3110178B1 (en) * 2020-05-18 2022-05-27 Bionobis Genotyping process suitable for the simultaneous treatment of a large number of patients

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2587606C2 (en) 2010-06-30 2016-06-20 БиДжиАй Дженомикс Ко., Лтд Novel method for pcr-sequencing and use thereof for hla genotyping
WO2016138080A1 (en) * 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins

Also Published As

Publication number Publication date
WO2021048504A1 (en) 2021-03-18
EP4028548A1 (en) 2022-07-20
FR3100820A1 (en) 2021-03-19

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