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FR3038616B1 - Procede de purification et de concentration d'acides nucleiques. - Google Patents

Procede de purification et de concentration d'acides nucleiques. Download PDF

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Publication number
FR3038616B1
FR3038616B1 FR1556386A FR1556386A FR3038616B1 FR 3038616 B1 FR3038616 B1 FR 3038616B1 FR 1556386 A FR1556386 A FR 1556386A FR 1556386 A FR1556386 A FR 1556386A FR 3038616 B1 FR3038616 B1 FR 3038616B1
Authority
FR
France
Prior art keywords
purification
concentration
nucleic acids
complex
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1556386A
Other languages
English (en)
Other versions
FR3038616A1 (fr
Inventor
Celia Francois
Axelle Cadiere
Clement Faye
Laurent Garrelly
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gl Biocontrol
Original Assignee
Gl Biocontrol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gl Biocontrol filed Critical Gl Biocontrol
Priority to FR1556386A priority Critical patent/FR3038616B1/fr
Priority to PCT/EP2016/065879 priority patent/WO2017005754A1/fr
Publication of FR3038616A1 publication Critical patent/FR3038616A1/fr
Application granted granted Critical
Publication of FR3038616B1 publication Critical patent/FR3038616B1/fr
Active legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Procédé pour purifier et concentrer au moins un fragment d'acides nucléiques d'une taille supérieure à 20 pb par formation d'un complexe entre un acide nucléique et un polymère polycationique en phase homogène, fixation du complexe sur une résine échangeuse de cations et élution par agitation mécanique.
FR1556386A 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques. Active FR3038616B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1556386A FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.
PCT/EP2016/065879 WO2017005754A1 (fr) 2015-07-06 2016-07-05 Procede de purification et de concentration d'acides nucleiques

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1556386A FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.

Publications (2)

Publication Number Publication Date
FR3038616A1 FR3038616A1 (fr) 2017-01-13
FR3038616B1 true FR3038616B1 (fr) 2020-11-06

Family

ID=54478149

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1556386A Active FR3038616B1 (fr) 2015-07-06 2015-07-06 Procede de purification et de concentration d'acides nucleiques.

Country Status (2)

Country Link
FR (1) FR3038616B1 (fr)
WO (1) WO2017005754A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506103A (en) * 1993-11-16 1996-04-09 Hybridon, Inc. Method for detecting charged oligonucleotides in biological fluids
US6310199B1 (en) * 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
DE102005005467A1 (de) 2005-02-04 2006-08-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verbunde keramischer Hohlfasern, Verfahren zu deren Herstellung und deren Verwendung
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
FR2885130B1 (fr) 2005-04-28 2007-06-29 Centre Nat Rech Scient Procede de preparation de polylysines dendrimeres greffes
WO2008097342A2 (fr) * 2006-07-31 2008-08-14 Sigma-Aldrich Co. Compositions et procédés d'isolation de molécules biologiques
JP5899731B2 (ja) * 2011-09-13 2016-04-06 ソニー株式会社 核酸精製方法、核酸抽出方法、及び核酸精製用キット
CN113355321A (zh) * 2011-09-26 2021-09-07 凯杰有限公司 用于分离细胞外核酸的快速方法
CN104350152B (zh) * 2012-06-01 2017-08-11 欧米伽生物技术公司 选择性核酸片段回收

Also Published As

Publication number Publication date
FR3038616A1 (fr) 2017-01-13
WO2017005754A1 (fr) 2017-01-12

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