FR3012333A1 - LIQUID TOOTHPASTE COMPRISING A HYDROLISA OF CHICOREOUS LEAVES, IN PARTICULAR IN THE STADIUM OF ENDIVE - Google Patents

Abstract

The present invention relates to a liquid dentifrice comprising: - a foaming agent; a fluorinated compound capable of releasing fluorine ions; - a non-alcoholic preservative; and - water, Typically according to the invention, the dentifrice further comprises a hydrolyzate of chicory leaves.

Description

The present invention relates to a liquid dentifrice comprising a hydrolyzate of chicory leaves, especially in the endive stage. WO 95/22958 discloses a liquid dentifrice which contains water, a sweetener, glycerin sodium lauryl sulfate and silica particles. This toothpaste contains no antibacterial preservative. Its conservation is therefore limited in time and moreover, it does not provide any antibacterial action during its use. Its cleaning action comes solely from the mechanical action generated by the friction of the silica particles on the teeth.

EP0648110 discloses a liquid dentifrice which comprises as a preservative of ethyl alcohol. This type of product tends to provide a burning sensation during its use, because of the presence of ethyl alcohol in a relatively high concentration giving the product an antibacterial action.

EP 1 136 074 discloses the possible use of a particular chicory root extract which has good antibacterial and anti-inflammatory properties in a toothpaste or mouthwash. This extract has a dark color that gives the end product a redhibitory color for the user.

In addition, toothpastes, liquid or not, include more and more chemicals whose interactions are poorly known. It is therefore desirable to provide dentifrices that provide the same benefits as those existing but whose components are the most natural possible. An object of the present invention is to provide a new liquid toothpaste that remedies at least one of the aforementioned disadvantages related to liquid toothpastes. This object is achieved by means of a liquid toothpaste comprising - a foaming agent; a fluorinated compound capable of releasing fluorine ions; - A conservationist ; and water. Typically, according to the invention, the liquid dentifrice further comprises a chicory leaf hydrolyzate.

Indeed, it is the merit of the Applicant to have highlighted that a toothpaste as mentioned above has an antibacterial effect and provides a good cleaning teeth. Since the hydrolyzate is less concentrated in active ingredients extracted from the plant, it is less colored and its use as an active ingredient does not give taste or color to the toothpaste of the invention. It is also an excellent source of minerals and trace elements and helps moisturize the oral mucosa. Preferably, the hydrolyzate of the invention is obtained from fresh leaves, more particularly endive endive chicory leaves. Preferably, said hydrolyzate is obtained by hydro-distillation by microwave under pulsed vacuum. Preferably, the hydrolyzate is a chicory hydrolyzate Cichorium intybus endive stage. Advantageously, the mass percentage of said hydrolyzate is substantially greater than or equal to 0.5% and substantially less than or equal to 3%. Advantageously, said foaming agent is chosen from sulphated foaming agents, in particular sodium lauryl sulphate, sulphated castor oil, sodium lauroyl sarcosinate and babassuamidopropyl betaine. The preservative used for the toothpaste according to the invention is not limited. It may be an alcoholic preservative. Advantageously, said preservative is a non-alcoholic preservative which may advantageously be chosen from sodium benzoate, sorbic acid, potassium sorbate and calcium sorbate. This avoids the aforementioned burning sensation. Advantageously, the mass percentage of said non-alcoholic preservative is substantially less than or equal to 1.5% and preferably substantially less than or equal to 1%. Advantageously, the dentifrice according to the invention comprises, in addition, at least one sweetener chosen from sodium saccharinate, stevia extracts, polyols, isomaltulose, isomaltose and combinations of at least two of said sweeteners. Advantageously, the percentage by weight of said sweetener or said sweeteners is substantially greater than or equal to 0.1% and substantially less than or equal to 0.5%. Advantageously, the liquid dentifrice according to the invention also comprises a weak acid and / or a salt of a weak acid chosen from citric acid, acetic acid, malic acid, lactic acid, phosphoric acid, and combinations of at least two of said acids and / or salts. Preferably, the toothpaste according to the invention comprises citric acid. Citric acid has antibacterial properties. In addition, citric acid makes it possible to bring the pH of the dentifrice to a value adapted to the use of sodium benzoate as a preservative. Advantageously, the toothpaste according to the invention further comprises sorbitol and / or glycerine, in particular vegetable glycerin. Advantageously, the liquid dentifrice according to the invention contains, in addition, a flavor and / or a dye of natural origin. The present invention also relates to the use of a chicory leaf hydrolyzate, especially in endive stage, as an antibacterial agent. DEFINITIONS According to the present invention, a liquid dentifrice is defined as being a liquid composition or having a viscosity between the viscosity of a liquid and that of a paste. The toothpaste according to the invention therefore has a dynamic viscosity substantially less than or equal to 100 Pa.s and substantially greater than or equal to 10-3 Pa.s, advantageously of the order of 1.3 × 10-3 Pa.s. More prosaically, a liquid dentifrice is defined as being a fluid which, when it is used without dilution by the user, can be distributed homogeneously in the oral cavity of the latter either under the action of saliva or under combined action of saliva and cheek movements 30 contributing to the flow of fluids contained in the oral cavity, in the space formed by the latter.

A liquid toothpaste differs from a mouthwash in that it has, in particular, a foaming effect for better cleaning teeth. According to the invention, a hydrolyzate is defined as being an aqueous composition, obtained by hydrodistillation (also called "steam distillation") of fresh, preferably ground, plants. The hydrolyzate is obtained at the same time as the essential oils, the hydrolyzate being the aqueous phase, the essential oils the organic phase. The hydrolyzate according to the invention can be obtained by hydro-distillation, by hydro-distillation under vacuum (which makes it possible to lower the distillation temperature and thereby preserve the thermolabile compounds of the hydrolyzate) or by microwave hydrodistillation under pulsed vacuum. For the purposes of the present invention, the term "chicory" means ordinary wild chicory (Cichorium intybus L), cultivated chicory (Cichorium intybus subsp. Intybus convar Sativum), Brussels chicory or chicory (Cichorium intybus subsp. intybus convar (Foliosum) and all hybrid F1 varieties of the two cultivated varieties mentioned above. The hydrolyzate is preferably obtained from chicory to the endive stage, that is to say when the leaves form a head salad. The leaves are endive, white with slightly green tips. The endives used for the preparation of the toothpaste according to the invention are obtained by a usual endive culture method, that is to say a culture in the absence of light. Within the meaning of the present invention, a non-alcoholic preservative is defined as being a preservative whose molecule does not comprise an alcohol function. If the preservative is a mixture of molecules, none of said molecules contains alcohol function. The alcoholic or non-alcoholic preservative may allow the preservation of the product by its antimicrobial, anti-oxidant, anti-radical activity or because of a combination of these activities. The term "preservative" without precision on the amount of said preservative, means that the molecule or the active molecule combination as a preservative is present in a minimal amount that does not exceed the minimum amount to obtain the aforementioned activity for the preservation of the preservative. product. For the purposes of the present invention, a preservative is considered to be an antibacterial preservative if it avoids or limits the proliferation of bacteria / and / or yeasts and / or molds introduced into a medium containing said preservative; the amount of bacteria being less than that of the medium in which they are introduced. An antibacterial product is defined as a product having a biocidal activity when it is introduced into a medium containing bacteria and / or yeasts and / or molds; the quantity of said introduced product being lower than that of the medium in which it is introduced. EXAMPLES The present invention, its features and the various advantages thereof will become more apparent upon reading the following description which relates to two particular embodiments of the present invention, presented as descriptive but non-limiting examples. Tables I and II below group the respective compositions of two examples of toothpaste according to the invention. Example 1: Mint-flavored liquid toothpaste Table I Ingredient Mass fraction in toothpaste (%) Supplier Sodium lauryl sulfate 1.25 Univar Vegetable glycerin 5 verfilco sorbitol 2.5 IMCD Natural flavor 0.5 Mane and yarn chlorophyll / mint Stevia extract 0.1 IMCD ribaudiana Organic endive hydrolyzate 1 Agrival Sodium benzoate 1 Nactis Sodium fluoride 0.05 Verfilco (panreac) Citric acid qsp pH = 4.5 to 5.5 univar Sodium saccharinate 0.2 univar Food color green qs LCW Water qs Example 2: strawberry-flavored liquid toothpaste Table II Ingredient Mass fraction in toothpaste (%) Supplier sodium lauryl sulfate 1.25 Univar Vegetable glycerin 5 verfilco sorbitol 2.5 IMCD Natural flavor of strawberry 0.5 Mane and son Stevia extract 0.1 IMCD ribaudiana Strawberry hydrolyzate 5 Interactive Gariguette Organic endive hydrolyzate 1 Ag rival Benzoate of 1 Nactis sodium Sodium fluoride 0.05 Verfilco (panreac) Citric acid e qsp pH = 4.5 to 5.5 univar Saccharinate 0.2 univar sodium Food color red qsp LCW Water qsp In the two examples mentioned above, the bio endive hydrolyzate is a hydrolyzate of endive leaves (Cichorium intybus ) grown according to the standards of organic farming. 3 0 1 2 3 3 3 7 The strawberry hydrolyzate Gariguette refers to a Fragaria ananassa hydrolyzate. Evaluation of the antibacterial activity of the liquid dentifrice according to Example 2. Tests according to the EN 1040 standard (April 2006) were used in order to prove the antimicrobial activity of the dentifrice according to the invention. Strain (s) tested: Pseudomonas aeruginosa ATCC 15442 - 37 ° C Staphylococcus aureus ATCC 6538 - 37 ° C Concentration (s) of product: Pur (80% during test), 50% and 25% Time contact time: 5 min ± 10s Mixture aspect: Homogeneous Test method: Dilution - neutralization Neutralizer used: LT100 broth Test temperature: 20-25 ° C According to the aforementioned standard, a product is considered to have antibacterial activity when a reduction is obtained a reduction of at least 5 log.

Table III summarizes the results obtained for the strain Pseudomonas aeruginosa ATCC 15442. Table III Test suspensions and tests Test suspension N (CFU / mL) Test suspension NO = N / 10 (CFU / mL) Ig NO 3.5 .108 3.5.107 7.55 Product concentration (s) Ig Na assay Reduction Time of contact Na (CFU / mL) Ig R = Ig NO-Ig Na Pure at 5 min ± 10s <140 <2.15> 5.40 50% at 5 min ± 10s <140 <2.15> 5.40 25% at 5 min ± 10s 345 2.54 5.01 Table IV below summarizes the results obtained for strain Staphylococcus aureus - ATCC 6538.

Table IV Test Suspensions and Tests Test Suspension N (CFU / mL) Test Suspension NO = N / 10 (CFU / mL) Ig NO Product Concentration (s) Ig Na Test Reduction Time Na Contact (UFC / mL). Ig R = Ig NO-Ig Na Pure at 5 min ± 10s <140 <2.15> 5.45 50% at 5 min ± 10s <140 <2.15> 5.45 25% at 5 min ± 10s <140 <2.15> 5.45 Referring to the results cited in Tables III and IV, it is found that the liquid dentifrice according to the invention has a biocidal activity, especially antibacterial, even when diluted to 25%. Preserving toothpaste The toothpaste of Example 2 was used to carry out a shelf life test.

The following strains: Pseudomonas aeruginosa (Pa), Staphylococcus aureus (Sa), Escherichia coli (Ec), Candida albicans (Ca) and Aspergillus brasiliensis (Ab) were introduced into the toothpaste of Example 2 diluted 1/1 in order to obtain bacterial or yeast concentrations ranging from 0.1 to 3 × 10 8 CFU / mL. The test was carried out according to standard NF ISO 21149 for bacteria and according to standard NF ISO 16212 for yeasts and molds. After 28 days, the density of each of the aforementioned germs was less than 10 CFU / g, attesting that the product could be stored for at least 28 days without special precautions. Tests on Users A panel of users (21 in total, mean age 37 years, aged 21 to 59) used once a day the toothpaste of the invention (according to Example 2) without brushing. The liquid toothpaste of the invention was used by simple introduction into the oral cavity and then set in motion in said oral cavity of the liquid-saliva dentifrice mixture for 30 seconds. Users also used a dough toothpaste with mechanical brushing once a day. The duration of the test was 21 days.

After 21 days, none of the users showed any decay or other tooth enamel alteration. For a user who before the beginning of the test showed signs of gingivitis, a slight improvement was observed (gingivitis of intensity 3 decreased to 2.5).

In addition, 70% of users indicated that the feeling of freshness and cleanliness provided during the use of the product persisted. Consequently, it is deduced from the above-mentioned user test that the toothpaste according to the invention makes it possible to obtain the same results as toothpaste in the form of paste combined with mechanical brushing of the teeth.

Claims (9)

1. A liquid toothpaste comprising: - a foaming agent; a fluorinated compound capable of releasing fluorine ions; - A conservationist ; and water, characterized in that it further comprises a hydrolyzate of chicory leaves. 2. Dentifrice according to claim 1, characterized in that said hydrolyzate is a chicory leaf hydrolyzate endive stage. 3. Liquid dentifrice according to claim 1, characterized in that the mass percentage of said hydrolyzate is substantially greater than or equal to 0.5% and substantially less than or equal to 3% relative to the total mass of said dentifrice. 4. Liquid dentifrice according to any one of the preceding claims, characterized in that said hydrolyzate is selected from hydrolysates obtained by hydro distillation of chicory leaves and hydrolysates obtained by hydro-distillation by pulsed vacuum microwave chicory leaves . 5. Liquid dentifrice according to any one of the preceding claims, characterized in that the mass percentage of said non-alcoholic preservative is substantially less than or equal to 1.5% and preferably substantially less than or equal to 1%. 6. Liquid dentifrice according to any one of the preceding claims, characterized in that said foaming agent is chosen from sulphated foaming agents, in particular sodium lauryl sulphate, sulphated castor oil, sodium lauroyl sarcosinate and sodium sulphate. babassuamidopropyl betaine. 7. Dentifrice according to any one of the preceding claims, characterized in that said preservative is a non-alcoholic preservative and selected in particular from sodium benzoate, sorbic acid, potassium sorbate and calcium sorbate. 8. Dentifrice according to any one of the preceding claims, characterized in that it comprises, in addition, at least one sweetener selected from sodium saccharinate, stevia extracts, polyols, isomaltulose, isomaltose and combinations of at least two of said sweeteners. 9. Liquid dentifrice according to any preceding claim, characterized in that it further comprises a weak acid and / or a salt of a weak acid selected from citric acid, acetic acid, acid malic acid, lactic acid, phosphoric acid and combinations of at least two of said acids and / or salts.