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FR3073047B1 - Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon - Google Patents

Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon Download PDF

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Publication number
FR3073047B1
FR3073047B1 FR1760338A FR1760338A FR3073047B1 FR 3073047 B1 FR3073047 B1 FR 3073047B1 FR 1760338 A FR1760338 A FR 1760338A FR 1760338 A FR1760338 A FR 1760338A FR 3073047 B1 FR3073047 B1 FR 3073047B1
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sample
estimating
image
particles
plane
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FR1760338A
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English (en)
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FR3073047A1 (fr
Inventor
Pierre Blandin
Anais Ali-Cherif
Aurelien Daynes
Estelle Gremion
Damien Isebe
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Horiba ABX SAS
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Horiba ABX SAS
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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Priority to FR1760338A priority Critical patent/FR3073047B1/fr
Priority to US16/760,777 priority patent/US11385158B2/en
Priority to PCT/FR2018/052689 priority patent/WO2019086800A1/fr
Publication of FR3073047A1 publication Critical patent/FR3073047A1/fr
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0227—Investigating particle size or size distribution by optical means using imaging; using holography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/0005—Adaptation of holography to specific applications
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
    • G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/60—Analysis of geometric attributes
    • G06T7/62—Analysis of geometric attributes of area, perimeter, diameter or volume
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/012—Red blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0205—Investigating particle size or size distribution by optical means
    • G01N15/0227—Investigating particle size or size distribution by optical means using imaging; using holography
    • G01N2015/0233—Investigating particle size or size distribution by optical means using imaging; using holography using holography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1434—Optical arrangements
    • G01N2015/1454—Optical arrangements using phase shift or interference, e.g. for improving contrast
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/0005—Adaptation of holography to specific applications
    • G03H2001/005—Adaptation of holography to specific applications in microscopy, e.g. digital holographic microscope [DHM]
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0447—In-line recording arrangement
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
    • G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
    • G03H2001/0883—Reconstruction aspect, e.g. numerical focusing
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H2210/00—Object characteristics
    • G03H2210/50—Nature of the object
    • G03H2210/55—Having particular size, e.g. irresolvable by the eye
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

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  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Geometry (AREA)
  • Signal Processing (AREA)
  • Computing Systems (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Holo Graphy (AREA)

Abstract

L'invention est un procédé d'estimation d'un volume représentatif de particules d'intérêt (10i) baignant dans un échantillon, l'échantillon s'étendant selon au moins un plan, dit plan de l'échantillon (P10), l'échantillon comportant un agent sphérisant, apte à modifier la forme des particules, le procédé comportant les étapes suivantes : a) illumination de l'échantillon à l'aide d'une source de lumière (11), la source de lumière émettant une onde lumineuse incidente (12) se propageant vers l'échantillon (10) selon un axe de propagation (Z) ; b) acquisition, à l'aide d'un capteur d'image (16), d'une image (I0) de l'échantillon (10), formée dans un plan de détection (P0), l'échantillon étant disposé entre la source de lumière (11) et le capteur d'image (16), chaque image étant représentative d'une onde lumineuse (14) dite d'exposition, à laquelle est exposé le capteur d'image (16) sous l'effet de l'illumination ; c) utilisation de l'image de l'échantillon (I0), acquise lors de l'étape b), et d'un opérateur de propagation holographique, pour calculer une expression complexe (A(x,y, z)) de l'onde lumineuse d'exposition (14) en différentes positions par rapport au plan de détection; le procédé comportant une étape d'estimation du volume représentatif (V) des particules d'intérêt (10i) en fonction des expressions complexes calculées lors de l'étape c)
FR1760338A 2017-11-02 2017-11-02 Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon Active FR3073047B1 (fr)

Priority Applications (3)

Application Number Priority Date Filing Date Title
FR1760338A FR3073047B1 (fr) 2017-11-02 2017-11-02 Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon
US16/760,777 US11385158B2 (en) 2017-11-02 2018-10-30 Optical method for estimating a representative volume of particles present in a sample based on a size of each region of interest
PCT/FR2018/052689 WO2019086800A1 (fr) 2017-11-02 2018-10-30 Procédé optique d'estimation d'un volume représentatif de particules présentes dans un échantillon

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1760338A FR3073047B1 (fr) 2017-11-02 2017-11-02 Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon
FR1760338 2017-11-02

Publications (2)

Publication Number Publication Date
FR3073047A1 FR3073047A1 (fr) 2019-05-03
FR3073047B1 true FR3073047B1 (fr) 2021-01-29

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FR1760338A Active FR3073047B1 (fr) 2017-11-02 2017-11-02 Procede optique d'estimation d'un volume representatif de particules presentes dans un echantillon

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US (1) US11385158B2 (fr)
FR (1) FR3073047B1 (fr)
WO (1) WO2019086800A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021055732A1 (fr) * 2019-09-20 2021-03-25 Kansas State University Research Foundation Procédés et appareil d'imagerie orthographique sans contact de particules d'aérosol
JP2023006004A (ja) * 2021-06-30 2023-01-18 株式会社エビデント 観察装置
FR3131376B1 (fr) * 2021-12-29 2023-11-10 Commissariat Energie Atomique Procédé d’estimation d’une distribution spatiale tridimensionnelle de fluorescence, à l’intérieur d’un objet.

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3382331A (en) 1966-11-30 1968-05-07 Gen Electric Circuit breaker rotary handle mechanism cam lock
US5284771A (en) 1991-12-05 1994-02-08 Miles Inc. Reagent compositions and their use in sphering cells
GB0701201D0 (en) 2007-01-22 2007-02-28 Cancer Rec Tech Ltd Cell mapping and tracking
JP5639654B2 (ja) * 2009-10-20 2014-12-10 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア オンチップでの非干渉性のレンズフリーのホログラフィおよび顕微鏡法
ES2438841T3 (es) 2009-12-31 2014-01-20 Abbott Point Of Care, Inc. Método y aparato para determinar el volumen celular medio de los glóbulos rojos en la sangre
JP5352763B2 (ja) * 2010-01-22 2013-11-27 公立大学法人兵庫県立大学 複素振幅インラインホログラムの生成方法および該方法を用いる画像記録装置
US20130260396A1 (en) * 2010-05-25 2013-10-03 Arryx, Inc. Holographic fluctuation microscopy apparatus and method for determining mobility of particle and/or cell dispersions
SG10201707599WA (en) * 2013-03-15 2017-10-30 Richard Harry Turner A System And Methods For The In Vitro Detection Of Particles And Soluble Chemical Entities In Body Fluids
JP6503382B2 (ja) * 2014-06-16 2019-04-17 シーメンス・ヘルスケア・ダイアグノスティックス・インコーポレーテッドＳｉｅｍｅｎｓ Ｈｅａｌｔｈｃａｒｅ Ｄｉａｇｎｏｓｔｉｃｓ Ｉｎｃ． 血液学用デジタルホログラフィ顕微鏡検査データ分析
FR3030749B1 (fr) 2014-12-19 2020-01-03 Commissariat A L'energie Atomique Et Aux Energies Alternatives Methode d'identification de particules biologiques par piles d'images holographiques defocalisees
FR3034196B1 (fr) 2015-03-24 2019-05-31 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede d'analyse de particules
FR3049348B1 (fr) 2016-03-23 2023-08-11 Commissariat Energie Atomique Procede de caracterisation d’une particule dans un echantillon
FR3049347B1 (fr) 2016-03-23 2018-04-27 Commissariat A L'energie Atomique Et Aux Energies Alternatives Procede d’observation d’un echantillon par calcul d’une image complexe

Also Published As

Publication number Publication date
US20200326269A1 (en) 2020-10-15
US11385158B2 (en) 2022-07-12
FR3073047A1 (fr) 2019-05-03
WO2019086800A1 (fr) 2019-05-09

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