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FR3051482B1 - Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique - Google Patents

Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique Download PDF

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Publication number
FR3051482B1
FR3051482B1 FR1654459A FR1654459A FR3051482B1 FR 3051482 B1 FR3051482 B1 FR 3051482B1 FR 1654459 A FR1654459 A FR 1654459A FR 1654459 A FR1654459 A FR 1654459A FR 3051482 B1 FR3051482 B1 FR 3051482B1
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FR
France
Prior art keywords
quantification
biological sample
microorganism
sequencing
determining
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Application number
FR1654459A
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English (en)
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FR3051482A1 (fr
Inventor
Samuel Gabillard
Virginie Chesnais
Alban Ott
Emmanuel Chaplais
Eric Ginoux
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life and Soft a Seqone Company SAS
Original Assignee
Life and Soft SAS
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Publication date
Application filed by Life and Soft SAS filed Critical Life and Soft SAS
Priority to FR1654459A priority Critical patent/FR3051482B1/fr
Priority to PCT/FR2017/051204 priority patent/WO2017198956A1/fr
Publication of FR3051482A1 publication Critical patent/FR3051482A1/fr
Application granted granted Critical
Publication of FR3051482B1 publication Critical patent/FR3051482B1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention porte sur un procédé de détermination de la présence et quantification d'au moins un micro-organisme dans un échantillon biologique humain comprenant des acides nucléiques totaux, comportant les étapes suivantes : - extraction des acides nucléiques totaux dudit échantillon biologique - séquençage haut débit desdits acides nucléiques totaux - traitement informatique des données de séquençage - le filtrage des séquences non humaines par alignement de séquences avec les séquences du génome de référence d'au moins un micro-organisme d'au moins un échantillon de référence - le calcul de la profondeur de séquençage du génome de référence d'au moins un micro-organisme - la détermination d'un indicateur de la quantification d'au moins un micro-organisme fonction de ladite profondeur de séquençage du génome de référence. La présente invention porte également sur un équipement et des kits pour la mise en œuvre dudit procédé, pour le diagnostic prénatal ou de cancers.
FR1654459A 2016-05-19 2016-05-19 Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique Active FR3051482B1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR1654459A FR3051482B1 (fr) 2016-05-19 2016-05-19 Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique
PCT/FR2017/051204 WO2017198956A1 (fr) 2016-05-19 2017-05-18 Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1654459A FR3051482B1 (fr) 2016-05-19 2016-05-19 Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique

Publications (2)

Publication Number Publication Date
FR3051482A1 FR3051482A1 (fr) 2017-11-24
FR3051482B1 true FR3051482B1 (fr) 2021-01-29

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
FR1654459A Active FR3051482B1 (fr) 2016-05-19 2016-05-19 Procede de determination de la presence et quantification d'au moins un micro-organisme dans un echantillon biologique

Country Status (2)

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FR (1) FR3051482B1 (fr)
WO (1) WO2017198956A1 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114420213B (zh) * 2021-12-31 2024-09-17 圣湘生物科技股份有限公司 一种生物信息分析方法及装置、电子设备及存储介质

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599216A (en) * 1983-11-21 1986-07-08 Board Of Regents For The University Of Oklahoma Apparatus for exposure to microwaves
US20130316331A1 (en) * 2011-01-26 2013-11-28 Ramot At Tel-Aviv University Ltd. Detection of infection by a microorganism using small rna sequencing subtraction and assembly
WO2014019275A1 (fr) 2012-07-31 2014-02-06 Bgi Shenzhen Co., Limited Détection non invasive de l'état de santé fœtal
KR102205950B1 (ko) * 2013-11-07 2021-01-22 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 인간 마이크로바이옴 및 그의 성분의 분석을 위한 무세포 핵산
CN105132584B (zh) * 2015-08-03 2018-06-29 中国人民解放军成都军区总医院 用于分型检测水痘带状疱疹病毒的试剂盒及其生产方法与应用

Also Published As

Publication number Publication date
FR3051482A1 (fr) 2017-11-24
WO2017198956A1 (fr) 2017-11-23

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