FR2847907A1 - Producing unicellular algae, diatoms, enriched in iodine, useful in topical compositions for slimming or treatment of cellulitis, comprises growing in a medium containing a specific content of iodine - Google Patents

Abstract

Producing unicellular algae (A), of the diatom type, enriched in iodine comprises culturing them in a medium of iodine content 0.1-13 mg/l. Independent claims are also included for the following: (1) (A) prepared by the new process; (2) iodine-containing extracts, aqueous, aqueous-alcoholic or aqueous-glycolic, of (A); and (3) cosmetic composition containing (A) or the extract of (2).

Description

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 The present invention relates to a process for obtaining unicellular algae of the diatom class, enriched in iodine.

 The accumulation of iodine by brown algae (class of phaeophyceae) or by red algae (class of rhodophyceae) in marine environment is known practically since the discovery of iodine by Courtois in 1811 in kelp. The iodine content of food algae is regularly monitored to verify compliance with the legislation in force in France (algal iodine, precision on the nutritional and regulatory aspects, Algorythme 23: 9-10 (1995)).

 In contrast, the iodine content of unicellular algae is poorly documented, and is a priori insufficient.

 However, it is important to have unicellular algae with iodine content not only well determinable, but also superior to known algae cultures.

 Such ants would indeed have an interest in various fields, particularly in the agri-food field by the nutritional supply of iodine, but also in the cosmetics field, iodine being known as acting on the metabolism of fats.

 The authors of the present invention have discovered that the cultivation of unicellular microalgae in specific culture media makes it possible to obtain an enrichment of the iodine content of the unicellular microalgae considered.

 The subject of the present invention is therefore a process for obtaining unicellular algae from the class of diatoms enriched in iodine, this process comprising the cultivation of unicellular algae of the diatom class in a culture medium having a concentration of iodine of 0.1 to 13 mg / l, which results in an iodine enrichment of these algae.

 The source of iodine may be of natural origin (for example, by adding algae extracts, as described below), or of synthetic origin (for example, by the addition of iodides, as described below ), or consist of a combination of source (s) originnaturelle and source (s) synthetic.

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 The iodine may in particular be provided in the form of iodide, preferably in the form of Mg12, NaI or KI. It can also be provided in the form of triethanolamine hydroiodide.

 Iodine can also be provided by natural extracts of algae, for example by an extract of kelp, fucus, levringia, pelvetia, caulerpe, ulva, enteromorpha, cladophora, gracillaria, chondrus, and corallina. Such an extract may be of aqueous, aqueous-alcoholic type (for example by use of ethanol, propanol or isopropanol), or glycolic (for example by using propylene glycol, butylene glycol, glycerol, ethyldiglycol, tripropylene glycol methyl ether, methylpropane diol, or neopentyl glycol).

 The water used to prepare these algae extracts is sweet or salty, for example at a percentage of 40 to 100% (by weight). The solvents can be used alone or in combination. In particular, non-ethereal polyols and monoethers of polyols may be combined.

 Preferably, the total iodine content of these extracts is 0.1 to 10,000 mg / l.

FeCl3 0, 5-10 mu/1 Zn Cl2 5-100 mg/l

COCI2, 6H20 5-100 mg/1 Na2 Mo04, 2H20 2-200 mg/l CUS04, 5H20 5-100 mg/l Thiamine 2-50 mg/l Pyridoxine 2-50 mg/l A preferred standard culture medium comprises:
Seawater (total salinity 10-70 g / l) qs-1000 ml
Na2EDTA 30-60 mg
H3BO3 20-40 mg
NaNO3 50-200 mg
NaH2PO4 10-100 mg
MnCl2 0.1-1 mg

FeCl3 0.5-10 mu / 1 Zn Cl2 5-100 mg / l

COCI2, 6H20 5-100 mg / l Na2 Mo04, 2H20 2-200 mg / l CUS04, 5H20 5-100 mg / l Thiamine 2-50 mg / l Pyridoxine 2-50 mg / l

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Cyanocobalamin 0.01-1 mg / l
Biotin 0.01-1 mg / l
Na2SiO3 0.2-20 mg / l
The unicellular algae considered, of the diatom class, include the thalassiosiraceae (thalassiosira, skeletonema, planktoniella), fragilariaceae (fragilaria, thalassiothrix, thalassionema, asterionella), auriculate (auricula), lithodesmiaceae (lithodesmium), eupodiscaceae (odontella) families.

 The culture conditions are known to those skilled in the art. For example, the culture method can be carried out under the following conditions: cultivation of the algal strain in a container, for example of the test tube type, for 15 days at 22 ° C., under permanent light or in alternating illumination / darkness cycle; transfer into larger volume containers, for example Erlenmeyer 250-500-1000 flask then 5000 ml (26 days), then 20 * flask (6 days) then 200 I flask (7 days), at each times under bubbling air / CO2 at 0.5-5%.

 An object of the invention is also the unicellular algae enriched in iodine, obtainable by the above culture method, and in particular algae which have an iodine content of about 150 to 200 mg / kg. These algae can be whole in suspension, or be in the form of crushed seeds. The invention also relates to the rich iodine (iodine) extracts of these unicellular algae. These extracts can be prepared in the same way as for the macroscopic algae used as iodine feed for the culture media, as described above.

 The invention also relates to cosmetic compositions comprising unicellular algae enriched in iodine, which can be obtained according to the process described above or the extracts of these algae. The cosmetic compositions according to the present invention preferably comprise from 0.1 to 70% by weight of unicellular algae in suspension (with an algae solids content of between 1 and 20% (by weight)).

 Advantageously, the concentration of unicellular algae is between 106 and 4 × 10 9 cells / ml.

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 The high salinity of the algal biomasses according to the invention not being an obstacle to the preparation of the cosmetic compositions, they are preferably in the form of a simple oil-in-water or water-in-water emulsion. oil, or a multiple emulsion, microemulsion, aqueous or hydroalcoholic gel, cream, oil, aqueous or hydroalcoholic lotion, whose viscosities can vary from 1 mPa.s to 400,000 mPa.s.

 In addition, the invention relates to a cosmetic treatment method, comprising the topical application of a cosmetic composition as defined above.

 These cosmetic compositions are particularly advantageous for the stimulation of the metabolism of fats, in particular slimming and anti-cellulite treatments, and massage creams.

 The following examples illustrate the invention without limiting its scope.

Example 1: culture of the cellular alga Thalassiosira eccentrica
Culture centre
KN03 100mg H3BO3 33mg KH2PO4, 20mg H20
Na2SiO3, 9H2O 26mg
Na2EDTA 45 mg
FeCl3, 6H20 1.3mg
MnCl 2, 4H 2 O 0.36 mg CuSO 4, 5H 2 O 0.020 mg
Zn S04, 7H20 0.021 mg
COCI2, 6H20 0.020 mg
NaMoO4, 2H2O 0.009 mg
Thiamine HCI 0.01 mg
Biotin 0.0001 mg
Cyahocobalamin 0.0001 mg
Pyridoxine 0.01 mg

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Laminar extract dosed at 1.75 g / l of iodine per liter of extract 2.5 g
0.2 m filtered seawater
35 g / l of total salinity qs 1 kg
The alga is kept in culture for two weeks in tanks of 200 1 with stirring by bubbling an air-carbon dioxide mixture under permanent lighting. The culture is then centrifuged. The biomass thus obtained is analyzed to determine the iodine content.

 The Thalassiosira alga grown on the medium as described above has an iodine content of 176 mg / kg.

 In comparison, the Thalassiosira alga grown on a conventional phytoplankton medium has an iodine content of only 6 mg / kg (the iodine contents being determined in relation to the dry weight of the seaweed).

Example 2 vaporizable lotion (in% w / w)
Demineralized water 46,72
Laminaria extract (Laminaria digitata) 10
Fucus extract (Fucus vesiculosus) 5
Suspension of Thallassiosira eccentrica at 1.106 cells / ml 15
Theophylline 0.1
Tetrasodium EDTA 0.4
PPG-3 methyl ether 5
Methylpropanediol 8
Oleth-20 0.8
Octyldodecaneth-30 1,2
Perfume 0.2
Ethanol 7
Phenoxyethanol 0.3
Methylparaben 0.2
Propylparaben 0.05
O-cymenol 0.03

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EXAMPLE 3 Slimming gel (in% weight / weight) Partially desalinated seawater (17 g / l) 45.4 Acrylates / C 10-30 Crosslinked acrylate polymer 1.2 Carrageenan 0.6 Sodium hydroxide 0.3 Ethylenediamine tetrahydroxypropyl 3 Glycerin 3 Methylpropanediol 7 Phenoxyethanol 0.3 Methylparaben 0.3 Propylparaben 0.07 Butylparaben 0.03 Ethanol 3 Hydrogenated castor oil PEG-40 1 Octyldodecaneth-30 1.5 Perfume 0.3 Suspension of Odontella aurita at 4.106 cells / ml 10 Laminaria extract (Laminaria hyperborea) 10 Enteromorpha extract (Enteromorpha flexusosa) 10
Asparagopsis extract (Asparagopsis armata) 3
Example 4: face slimming milk (% w / w)
Partially desalinated seawater (15 g / l) 51.55
Methylpropanediol 5
Phenoxyethanol 0.3
Methylparaben 0.3
Propylparaben 0.1
Butylparaben 0.04
Laminaria extract (Laminaria hyperborea) 2

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 Asterionella japonica suspension at 4.106 cells / ml 10 Glycerin 3 Xanthan gum 0.2 Acrylates / C 10-30 crosslinked polymer alkyl acrylate 0.6 Ethyl hexyl cocoate 3 Shea butter 2 Sorbitan stearate 1.8 Cetyl alcohol 3 Ceteth -20 1,4 Cyclopentasiloxam 4 Glyceryl Stearate 1,3 Caprylate / Coconut Caprate 3 Linoleic Acid 0.6 Retinyl Palmitate 0.05 Tocopherol 0.1 Fish Collagen 0.5 Fragrance 0.4 Titanium Oxide 2 Oxide red iron 0.1 Yellow iron oxide 0.6 Black iron oxide 0.06 Example 5: slimming cream (% w / w) Demineralized water 44.17 Disodium EDTA 0.3 Butylene glycol 3 Methylpropanediol 5 Ethanol Phenoxyethanol 0, 3 Methylparaben 0.2 Propylparaben 0.07 Butylparaben 0.03

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 Carbomer 0.6 Acrylates / crosslinked polymer vinylisodecanoate 0.2 Tetrahydroxypropylethylenediamine 0.6 Arginine 0.2 Sodium hydroxide 0.003 PEG-100 stearate 2 Cetearyl ethyl hexanoate 4 Dicaprylyl carbonate 5 Hydrogenated palm oil 2 Shea butter 1 Cetyl alcohol 5 Octyldodecanol 3 PEG-8 stearate 1 Beta sitosterol 1 Lecithin (soybean) 0.5 Polyacrylamide 0.4 Perfume 0.4 Suspension of Ondotella aurita at 1.106 cells / ml Methylsilanol sodium mannuronate Example 6: slimming cream (% by weight) weight) Demineralized water 33.43 Mother liquor (Chorumag 50, Compagnie des salins du Midi) 2 Suspension of Odontella aurita at 4.106 cells / ml PPG 3 methyl ether 3 Methylpropanediol 5 Methylparaben 0.3 Propylparaben 0.1 O-cymenol 0, 07 PCA 0.2 Panthenol 1 Guar hydroxypropyltrimonium guar 0.7 Silica 2

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PEG-50 Stearate 1.9 PEG-8 Stearate 1.6 Glyceryl Stearate 3.2 Pentaerythrityl Stearate 4 Squalane 4 Cocoate Ethyl Hexyl 7 Cyclopentasiloxam 5 Betasitosterol 0.8 Linoleic Acid 0.2 Jojoba Oil 0.2 Hexadecane 9 Perfume 0.3
Methylsilanol sodium mannuronate Example 7: massage cream (in% weight / weight) Partially desalinated seawater 39.4 Thalassiosira eccentrica suspension at 4.106 cells / ml Methylpropanediol 5 PEG-4 3 Midazolidinulurea 0.3 Methylparaben 0.2 Propylparaben 0.07 Butylparaben 0.03 Cetyldimethicone Copolyol 3 Hydrogenated castor oil PEG-7 0.9 PEG-45 / dodecylglycol copolymer 1.6 Sorbitan sesquioleate 3 Mineral oil 7 Petrolatum 5 Hexyldecanol 1 Glyceryl linoleate 1 Hydrogenated castor oil 2

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 Dimethicone 1 Squalane 3 Cyclopentasiloxame 3 Perfume 0.5

Claims (14)

1. Process for obtaining unicellular algae of the diatom class enriched in iodine, process comprising the cultivation of unicellular algae of the diatom class in a culture medium having an iodine concentration of 0.1 to 13 mg / This results in an iodine enrichment of these algae.  2. The method of claim 1, wherein the iodine is provided in the form of iodide.  3. The process according to claim 2, wherein the iodine is in the form of Mg12, NaI, KI, or triethanolamine hydroiodide.  4. Process according to claim 1, in which the iodine is provided in the form of an aqueous, hydroalcoholic or hydroglycolic extract chosen from an extract of laminaria, fucus, levringia, pelvetia, caulerpe, ulva, enteromorpha, cladophora, gracillaria, chondrus, and corallina.  5. Method according to one of claims 1 to 4, wherein the culture medium comprises: Seawater (total salinity 10-70 g / l) qs-1000 ml Na2EDTA 30-60 mg H3B03 20-40 mg NaNO3 50-200 mg NaH2PO4 10-100 mg MnCl2 0.1-1 mg FeCl3 0.5-10 mg / l Zn Cl2 5-100 mg / l

 COCI2, fiH20 5-100 mg / l Na2MoO4, 2H2O 2-200 mg / l CUSO4, 5H2O 5-100 mg / l <Desc / Clms Page number 12> Na2SiO3 0.2-20 mg / l Biotin 0.01-1 mg / l Cyanocobalamin 0.01-1 mg / l Pyridoxine 2-50 mg / l Thiamine 2-50 mg / l 6. Unicellular algae obtainable by the process of claims 1 to 5.  Algae according to claim 6 which have an iodine content of about 150 to 200 mg / kg.  8. Aqueous, hydroalcoholic or hydroglycolic iodinated extract of an algae as defined in claim 6 or 7.  9. The algae extract as claimed in claim 8, comprising a total iodine content of 0.1 to 10,000 mg / l. Cosmetic composition comprising unicellular algae of claim 6 or 7.  The composition of claim 10, wherein the unicellular algae is in suspension and included at a percentage of 0.1 to 70% by weight of the composition.  The composition of claim 10 or 11, wherein said unicellular algae are present at a concentration of 10 6 to 4 x 10 9 cells / ml.  13. Cosmetic composition comprising an extract of algae as defined in one of claims 8 or 9. <Desc / Clms Page number 13>  14. Cosmetic treatment method with slimming effect, comprising the topical application of a cosmetic composition as defined in one of claims 10 to 13.