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FR2693207B1 - Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes. - Google Patents

Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes.

Info

Publication number
FR2693207B1
FR2693207B1 FR929208254A FR9208254A FR2693207B1 FR 2693207 B1 FR2693207 B1 FR 2693207B1 FR 929208254 A FR929208254 A FR 929208254A FR 9208254 A FR9208254 A FR 9208254A FR 2693207 B1 FR2693207 B1 FR 2693207B1
Authority
FR
France
Prior art keywords
cytochrome
bioconversion
reductase
endogenous
heterogeneous
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
FR929208254A
Other languages
French (fr)
Other versions
FR2693207A1 (en
Inventor
Michael Kazmaier
Denis Pompon
Claudia Mignotte Vieux
Hermann Teutsch
Danielle Werck-Reichart
Michel Renaud
Kazmaier Michael
Pompon Denis
Mignotte Vieux Claudia
Teutsch Hermann
Werck-Reichart Danielle
Renaud Michel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Orsan
Original Assignee
Orsan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orsan filed Critical Orsan
Priority to FR929208254A priority Critical patent/FR2693207B1/en
Priority to PCT/FR1993/000676 priority patent/WO1994001564A1/en
Publication of FR2693207A1 publication Critical patent/FR2693207A1/en
Application granted granted Critical
Publication of FR2693207B1 publication Critical patent/FR2693207B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/13—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen (1.14.13)
    • C12Y114/13011—Trans-cinnamate 4-monooxygenase (1.14.13.11)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
FR929208254A 1992-07-03 1992-07-03 Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes. Expired - Lifetime FR2693207B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR929208254A FR2693207B1 (en) 1992-07-03 1992-07-03 Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes.
PCT/FR1993/000676 WO1994001564A1 (en) 1992-07-03 1993-07-02 Yeast strain for the co-expression of a plant cytochrome p450 mono-oxygenase activity and an endogenous or heterologous nadph-cytochrome p450-reductase, and use thereof in bioconversion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR929208254A FR2693207B1 (en) 1992-07-03 1992-07-03 Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes.

Publications (2)

Publication Number Publication Date
FR2693207A1 FR2693207A1 (en) 1994-01-07
FR2693207B1 true FR2693207B1 (en) 1994-09-30

Family

ID=9431533

Family Applications (1)

Application Number Title Priority Date Filing Date
FR929208254A Expired - Lifetime FR2693207B1 (en) 1992-07-03 1992-07-03 Yeast strain allowing the co-expression of a heterogeneous cytochrome P450 monooxygenase activity and an endogenous or heterologous NADPH-cytochrome P450-reductase and its use for bioconversion purposes.

Country Status (2)

Country Link
FR (1) FR2693207B1 (en)
WO (1) WO1994001564A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19507546C2 (en) * 1995-03-03 2001-05-03 Max Delbrueck Centrum Process for the regioselective hydroxylation of long-chain alkanes, fatty acids and other alkyl compounds
GB9606187D0 (en) 1996-03-23 1996-05-29 Inst Of Food Research Production of vanillin
US6787337B1 (en) 1997-09-24 2004-09-07 Aventis Cropscience S.A. Recording of DNA sequences to enable them to be expressed in yeasts, and the transformed yeasts obtained
FR2768748B1 (en) * 1997-09-24 2001-06-08 Rhone Poulenc Agrochimie RECODING OF DNA SEQUENCES ALLOWING THEIR EXPRESSION IN YEAST AND PROCESSED YEAST OBTAINED
GB9825421D0 (en) 1998-11-19 1999-01-13 Isis Innovation Process for oxidising terpenes
GB9914373D0 (en) 1999-06-18 1999-08-18 Isis Innovation Process for oxidising aromatic compounds
US6464986B1 (en) * 2000-04-14 2002-10-15 Allegan Sales, Inc. Method for treating pain by peripheral administration of a neurotoxin
EP1597364B1 (en) 2003-02-18 2013-07-24 Metabolic Explorer Method for the production of evolved microorganisms which permit the generation or modification of metabolic pathways
GB0719620D0 (en) 2007-10-08 2007-11-14 Isis Innovation Mutant Enzymes

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
JPH062067B2 (en) * 1988-08-16 1994-01-12 工業技術院長 Yeast NADPH-cytochrome P450 reductase producing strain
WO1991014781A1 (en) * 1990-03-19 1991-10-03 Henkel Research Corporation METHOD FOR INCREASING THE OMEGA-HYDROXYLASE ACTIVITY IN $i(CANDIDA TROPICALIS)

Also Published As

Publication number Publication date
WO1994001564A1 (en) 1994-01-20
FR2693207A1 (en) 1994-01-07

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