FI872753L - Method for activating genetically engineered heterologous eukaryotic proteins containing disulfide bridges after expression in prokaryotes - Google Patents

Method for activating genetically engineered heterologous eukaryotic proteins containing disulfide bridges after expression in prokaryotes Download PDF

Info

Publication number: FI872753L
Authority: FI; Finland
Prior art keywords: prokaryotes; expression; genetically engineered; disulfide bridges; proteins containing
Prior art date: 1985-10-23

Application number

FI872753A

Other languages

Finnish (fi)

Swedish (sv)

Other versions

FI94050C (en

FI872753A0 (en

FI94050B (en

Inventor

Rainer Rudolph

Stephan Fischer

Ralf Mattes

Original Assignee

Boehringer Mannheim Gmbh

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-10-23

Filing date

1987-06-22

Publication date

1987-06-22

1987-06-22 Application filed by Boehringer Mannheim Gmbh filed Critical Boehringer Mannheim Gmbh

1987-06-22 Publication of FI872753L publication Critical patent/FI872753L/en

1987-06-22 Publication of FI872753A0 publication Critical patent/FI872753A0/en

1993-09-03 Priority to FI933868A priority Critical patent/FI95578C/en

1995-03-31 Application granted granted Critical

1995-03-31 Publication of FI94050B publication Critical patent/FI94050B/en

1995-07-10 Publication of FI94050C publication Critical patent/FI94050C/en

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/565—IFN-beta
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Medicinal Chemistry (AREA)
Molecular Biology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biotechnology (AREA)
Chemical Kinetics & Catalysis (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Chemical & Material Sciences (AREA)
Analytical Chemistry (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Mechanical Treatment Of Semiconductor (AREA)
Lubricants (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)

FI872753A 1985-10-23 1987-06-22 Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes FI94050C (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
FI933868A FI95578C (en)	1985-10-23	1993-09-03	Method for Activation of Gene Technologist Produced, Heterologous, Eukaryotic Proteins Showing Disulfide Crosslinks after Expression in Prokaryotic Cells

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
DE19853537708 DE3537708A1 (en)	1985-10-23	1985-10-23	METHOD FOR ACTIVATING T-PA AFTER EXPRESSION IN PROKARYONTS
DE3537708		1985-10-23
EP8600610		1986-10-23
PCT/EP1986/000610 WO1987002673A2 (en)	1985-10-23	1986-10-23	Process for activating heterologous, eucaryotic proteins genetically engineered and presenting disulphide bridges after their expression in procaryotic cells

Publications (4)

Publication Number	Publication Date
FI872753L true FI872753L (en)	1987-06-22
FI872753A0 FI872753A0 (en)	1987-06-22
FI94050B FI94050B (en)	1995-03-31
FI94050C FI94050C (en)	1995-07-10

Family

ID=6284269

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
FI872753A FI94050C (en)	1985-10-23	1987-06-22	Method for activating gene-technologically produced heterologous eukaryotic proteins exhibiting disulfide crosslinks after expression in prokaryotes
FI933868A FI95578C (en)	1985-10-23	1993-09-03	Method for Activation of Gene Technologist Produced, Heterologous, Eukaryotic Proteins Showing Disulfide Crosslinks after Expression in Prokaryotic Cells

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
FI933868A FI95578C (en)	1985-10-23	1993-09-03	Method for Activation of Gene Technologist Produced, Heterologous, Eukaryotic Proteins Showing Disulfide Crosslinks after Expression in Prokaryotic Cells

Country Status (26)

Country	Link
EP (3)	EP0219874B1 (en)
JP (2)	JPH0728745B2 (en)
KR (1)	KR900009139B1 (en)
AT (2)	ATE131489T1 (en)
AU (2)	AU590029B2 (en)
CA (1)	CA1329157C (en)
CZ (1)	CZ280727B6 (en)
DD (1)	DD260517A5 (en)
DE (3)	DE3537708A1 (en)
DK (2)	DK175091B1 (en)
ES (2)	ES2020498T3 (en)
FI (2)	FI94050C (en)
GR (2)	GR920300062T1 (en)
HK (2)	HK153496A (en)
HR (1)	HRP921075B1 (en)
HU (2)	HU204855B (en)
IE (1)	IE62634B1 (en)
IL (1)	IL80325A (en)
PT (1)	PT83609B (en)
SI (1)	SI8611796B (en)
SK (1)	SK752686A3 (en)
SU (1)	SU1607689A3 (en)
UA (1)	UA6023A1 (en)
WO (1)	WO1987002673A2 (en)
YU (1)	YU47185B (en)
ZA (1)	ZA868012B (en)

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US4777043A (en) *	1985-12-17	1988-10-11	Genentech, Inc.	Stabilized human tissue plasminogen activator compositions
WO1988008428A1 (en) *	1987-04-28	1988-11-03	Amgen Inc.	Method for purifying granulocyte/macrophage colony stimulating factor
DE3722082A1 (en) *	1987-07-03	1989-01-12	Behringwerke Ag	METHOD FOR DETERMINING THE ACTIVITY OF SERINE PROTEASES OR SERINE PROTEASE INHIBITORS
CA1340586C (en) *	1988-09-23	1999-06-08	Cetus Corporation	Process for recovering microbially produced interferon-beta
DE3832898A1 (en) *	1988-09-28	1990-04-12	Boehringer Mannheim Gmbh	PRAEPARATE OF EXPRESSED PLASMINOGEN ACTIVATOR IN PROKARYONS
DE3835350A1 (en) *	1988-10-17	1990-04-19	Boehringer Mannheim Gmbh	ACTIVATION OF GENETICALLY MANUFACTURED ANTIBODY EXPRESSED IN PROKARYONS
DE3903581A1 (en) *	1989-02-07	1990-08-16	Boehringer Mannheim Gmbh	FABRIC PLASMINOGEN ACTIVATOR DERIVATIVE
DE3942143A1 (en) *	1989-12-20	1991-06-27	Boehringer Mannheim Gmbh	T-PA PRO STABILIZATION
JPH06500084A (en) *	1990-08-20	1994-01-06	ノボ　ノルディスク　アクティーゼルスカブ	Biologically active compounds, their production methods and their uses
EP0547102B1 (en) *	1990-09-05	1998-07-08	Southern Cross Biotech Pty.Ltd.	Solubilization of proteins in active forms
DE4037196A1 (en) *	1990-11-22	1992-05-27	Boehringer Mannheim Gmbh	METHOD FOR REACTIVATING DENATURED PROTEIN
DE4113750A1 (en)	1991-04-26	1992-10-29	Boehringer Mannheim Gmbh	IMPROVEMENT OF RENATURATION IN THE SECRETION OF DISULFID-BRIDGED PROTEINS
DE4139000A1 (en)	1991-11-27	1993-06-03	Boehringer Mannheim Gmbh	METHOD OF GENERATING BIOLOGICALLY ACTIVE SS-NGF
US5212091A (en) *	1992-03-02	1993-05-18	Monsanto Company	Method of producing tissue factor pathway inhibitor
CA2109820A1 (en) *	1992-03-24	1993-09-30	George N. Cox	Refolding and purification of insulin-like growth factor i
EP0600372B1 (en)	1992-12-02	1997-02-05	Hoechst Aktiengesellschaft	Process for the preparation of proinsulin with correctly linked cystin bridges
DE4405179A1 (en) *	1994-02-18	1995-08-24	Hoechst Ag	Method of obtaining insulin with correctly connected cystine bridges
FR2729972B1 (en) *	1995-01-31	1997-04-18	Sanofi Sa	PROCESS FOR THE EXTRACTION OF PERIPLASMIC PROTEINS FROM PROKARYOTIC MICROORGANISMS IN THE PRESENCE OF ARGININ
US5714371A (en) *	1995-05-12	1998-02-03	Schering Corporation	Method for refolding insoluble aggregates of hepatitis C virus protease
US5728804A (en) *	1995-06-02	1998-03-17	Research Corporation Technologies, Inc.	Use of cyclodextrins for protein renaturation
KR100305341B1 (en) *	1996-06-11	2002-03-02	로셰 디아그노스틱스 게엠베하	How to Activate Denatured Protein
US7153943B2 (en)	1997-07-14	2006-12-26	Bolder Biotechnology, Inc.	Derivatives of growth hormone and related proteins, and methods of use thereof
US6653098B1 (en)	1998-02-23	2003-11-25	G. D. Searle & Co.	Method of producing mouse and human endostatin
DE19850429A1 (en) *	1998-10-27	2000-05-04	Andre Schrattenholz	Fragments
EP1048732A1 (en) *	1999-04-26	2000-11-02	F. Hoffmann-La Roche Ag	Process for producing natural folded and secreted proteins
EP1077263A1 (en) *	1999-07-29	2001-02-21	F.Hoffmann-La Roche Ag	Process for producing natural folded and secreted proteins by co-secretion of chaperones
JP4873818B2 (en)	2000-05-16	2012-02-08	ボルダーバイオテクノロジー，インコーポレイテッド	Methods for refolding proteins containing free cysteine residues
DE10105912A1 (en) *	2001-02-09	2002-08-14	Roche Diagnostics Gmbh	Recombinant Proteinase K
DE10105911A1 (en)	2001-02-09	2002-08-14	Roche Diagnostics Gmbh	Expression of the recombinant proteinase K from Tritirachium album in yeast
DE102005033250A1 (en)	2005-07-15	2007-01-18	Bioceuticals Arzneimittel Ag	Process for purifying G-CSF
DE202006020194U1 (en)	2006-03-01	2007-12-06	Bioceuticals Arzneimittel Ag	G-CSF liquid formulation
CN101506222B (en)	2006-07-14	2013-10-02	健泰科生物技术公司	Refolding of recombinant proteins
WO2008076933A2 (en)	2006-12-14	2008-06-26	Bolder Biotechnology, Inc.	Long acting proteins and peptides and methods of making and using the same
NZ601759A (en)	2010-03-17	2013-07-26	Biogenerix Gmbh	Method for obtaining biologically active recombinant human g-csf
AU2011317021B2 (en) *	2010-10-20	2015-07-09	Medimmune, Llc	Methods for processing inclusion bodies
HUP1200172A2 (en)	2012-03-19	2013-10-28	Richter Gedeon Nyrt	Methods for refolding g-csf from inclusion bodies
HUP1200171A1 (en)	2012-03-19	2013-09-30	Richter Gedeon Nyrt	Methods for the production of polypeptides
CN103852527B (en) *	2012-12-05	2015-05-13	中国科学院大连化学物理研究所	High-flux protein sample pre-treatment device
US10457716B2 (en)	2014-08-06	2019-10-29	University Of Notre Dame Du Lac	Protein folding and methods of using same
CA3044371C (en)	2016-12-30	2024-01-16	Biogend Therapeutics Co., Ltd.	Recombinant polypeptides and nucleic acid molecules, compositions, and methods of making and uses thereof
HRP20251064T1 (en)	2020-12-18	2025-11-07	Richter Gedeon Nyrt.	METHODS FOR PURIFICATION OF REFOLDED FC-PEPTIDE FUSION PROTEIN

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1985
- 1985-10-23 DE DE19853537708 patent/DE3537708A1/en active Granted
1986
- 1986-10-10 IE IE268386A patent/IE62634B1/en not_active IP Right Cessation
- 1986-10-15 IL IL80325A patent/IL80325A/en not_active IP Right Cessation
- 1986-10-17 SK SK7526-86A patent/SK752686A3/en unknown
- 1986-10-17 CZ CS867526A patent/CZ280727B6/en not_active IP Right Cessation
- 1986-10-21 SI SI8611796A patent/SI8611796B/en unknown
- 1986-10-21 YU YU179686A patent/YU47185B/en unknown
- 1986-10-22 DD DD29546886A patent/DD260517A5/en not_active IP Right Cessation
- 1986-10-22 CA CA000521121A patent/CA1329157C/en not_active Expired - Lifetime
- 1986-10-22 ZA ZA868012A patent/ZA868012B/en unknown
- 1986-10-23 UA UA4202987A patent/UA6023A1/en unknown
- 1986-10-23 ES ES90109721T patent/ES2020498T3/en not_active Expired - Lifetime
- 1986-10-23 KR KR1019870700536A patent/KR900009139B1/en not_active Expired
- 1986-10-23 EP EP86114731A patent/EP0219874B1/en not_active Expired - Lifetime
- 1986-10-23 HU HU865290A patent/HU204855B/en unknown
- 1986-10-23 EP EP90109721A patent/EP0393725B1/en not_active Expired - Lifetime
- 1986-10-23 AT AT90109721T patent/ATE131489T1/en not_active IP Right Cessation
- 1986-10-23 PT PT83609A patent/PT83609B/en not_active IP Right Cessation
- 1986-10-23 WO PCT/EP1986/000610 patent/WO1987002673A2/en not_active Ceased
- 1986-10-23 JP JP61505882A patent/JPH0728745B2/en not_active Expired - Lifetime
- 1986-10-23 HU HU865290A patent/HUT43643A/en unknown
- 1986-10-23 DE DE86114731T patent/DE3689404D1/en not_active Expired - Lifetime
- 1986-10-23 AU AU65993/86A patent/AU590029B2/en not_active Ceased
- 1986-10-23 DE DE3650449T patent/DE3650449D1/en not_active Expired - Lifetime
- 1986-10-23 AT AT86114731T patent/ATE98648T1/en not_active IP Right Cessation
- 1986-10-23 ES ES86114731T patent/ES2061434T3/en not_active Expired - Lifetime
- 1986-10-23 EP EP86906320A patent/EP0253823A1/en active Pending
1987
- 1987-06-22 SU SU874202987Q patent/SU1607689A3/en active
- 1987-06-22 FI FI872753A patent/FI94050C/en not_active IP Right Cessation
- 1987-06-23 DK DK198703203A patent/DK175091B1/en not_active IP Right Cessation
1989
- 1989-09-13 AU AU41321/89A patent/AU607083B2/en not_active Expired
1991
- 1991-04-12 JP JP3079762A patent/JPH0824594B2/en not_active Expired - Lifetime
1992
- 1992-08-31 GR GR92300062T patent/GR920300062T1/en unknown
- 1992-10-16 HR HRP-1796/86A patent/HRP921075B1/en not_active IP Right Cessation
1993
- 1993-09-03 FI FI933868A patent/FI95578C/en not_active IP Right Cessation
1995
- 1995-12-14 GR GR950403376T patent/GR3018410T3/en unknown
1996
- 1996-08-08 HK HK153496A patent/HK153496A/en not_active IP Right Cessation
- 1996-08-08 HK HK153596A patent/HK153596A/en not_active IP Right Cessation
2000
- 2000-12-18 DK DK200001897A patent/DK175109B1/en not_active IP Right Cessation

Also Published As

Publication number	Publication date
IL80325A (en)	1992-06-21
DE3650449D1 (en)	1996-01-25
EP0219874A2 (en)	1987-04-29
EP0393725B1 (en)	1995-12-13
JPS62502895A (en)	1987-11-19
HK153496A (en)	1996-08-16
IE62634B1 (en)	1995-02-22
FI94050C (en)	1995-07-10
JPH0824594B2 (en)	1996-03-13
DK175109B1 (en)	2004-06-07
DK320387A (en)	1987-06-23
EP0393725A1 (en)	1990-10-24
SK278317B6 (en)	1996-10-02
HRP921075B1 (en)	1999-02-28
FI95578B (en)	1995-11-15
FI95578C (en)	1996-02-26
ES2061434T3 (en)	1994-12-16
CZ280727B6 (en)	1996-04-17
SI8611796A (en)	1996-10-31
JPH04218387A (en)	1992-08-07
JPH0728745B2 (en)	1995-04-05
CA1329157C (en)	1994-05-03
GR3018410T3 (en)	1996-03-31
HUT43643A (en)	1987-11-30
EP0253823A1 (en)	1988-01-27
HU204855B (en)	1992-02-28
FI933868L (en)	1993-09-03
KR900009139B1 (en)	1990-12-22
FI872753A0 (en)	1987-06-22
ATE131489T1 (en)	1995-12-15
IE862683L (en)	1987-04-23
SK752686A3 (en)	1996-10-02
WO1987002673A2 (en)	1987-05-07
EP0219874B1 (en)	1993-12-15
DE3537708A1 (en)	1987-04-23
ZA868012B (en)	1987-06-24
SU1607689A3 (en)	1990-11-15
AU590029B2 (en)	1989-10-26
AU607083B2 (en)	1991-02-21
AU6599386A (en)	1987-05-19
HK153596A (en)	1996-08-16
DK320387D0 (en)	1987-06-23
WO1987002673A3 (en)	1987-10-22
ATE98648T1 (en)	1994-01-15
SI8611796B (en)	1998-06-30
GR920300062T1 (en)	1992-08-31
EP0219874A3 (en)	1988-02-10
ES2020498T3 (en)	1996-04-01
IL80325A0 (en)	1987-01-30
DE3689404D1 (en)	1994-01-27
KR870700601A (en)	1987-12-30
FI94050B (en)	1995-03-31
YU179686A (en)	1988-06-30
DD260517A5 (en)	1988-09-28
ES2020498A4 (en)	1991-08-16
CZ752686A3 (en)	1996-01-17
DE3537708C2 (en)	1993-07-08
AU4132189A (en)	1990-01-04
UA6023A1 (en)	1994-12-29
DK200001897A (en)	2000-12-18
PT83609B (en)	1988-10-14
FI933868A0 (en)	1993-09-03
HRP921075A2 (en)	1995-06-30
DK175091B1 (en)	2004-05-24
PT83609A (en)	1986-11-01
YU47185B (en)	1995-01-31

Publication	Publication Date	Title
FI872753L (en)	1987-06-22	Method for activating genetically engineered heterologous eukaryotic proteins containing disulfide bridges after expression in prokaryotes
KR880700095A (en)	1988-02-15	Method for strengthening lead-antimony alloy
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