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ES8705467A1 - Un procedimiento para producir la proteina apoacuorina - Google Patents

Un procedimiento para producir la proteina apoacuorina

Info

Publication number
ES8705467A1
ES8705467A1 ES550528A ES550528A ES8705467A1 ES 8705467 A1 ES8705467 A1 ES 8705467A1 ES 550528 A ES550528 A ES 550528A ES 550528 A ES550528 A ES 550528A ES 8705467 A1 ES8705467 A1 ES 8705467A1
Authority
ES
Spain
Prior art keywords
protein
procedure
apoacuorine
producing
apoacuorin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES550528A
Other languages
English (en)
Other versions
ES550528A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Georgia
University of Georgia Research Foundation Inc UGARF
Original Assignee
University of Georgia
University of Georgia Research Foundation Inc UGARF
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Georgia, University of Georgia Research Foundation Inc UGARF filed Critical University of Georgia
Publication of ES550528A0 publication Critical patent/ES550528A0/es
Publication of ES8705467A1 publication Critical patent/ES8705467A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR LA PROTEINA APOACUORINA. COMPRENDE: A) SINTETIZAR UNA MOLECULA DE ADN QUE TIENE UNA SECUENCIA DE NUCLEOSIDOS QUE CODIFICA UN PEPTIDO DE FORMULA (I); B) RECOMBINAR LA MOLECULA DE ADN CON UN VECTOR, PARA OBTENER EL ADN VECTOR RECOMBINANTE PUC9; C) INSERTAR EL ADN VECTOR RECOMBINANTE EN E. COLI; D) CULTIVAR EL MICROORGANISMO E. COLI TRANSFORMADO; Y E) RECOGER LA PROTEINA APOACUORINA POR EL MICROORGANISMO TRANSFORMADO. SE UTILIZA EN ENSAYOS QUIMICOS O BIOQUIMICOS COMO MARCADOR.
ES550528A 1984-12-31 1985-12-30 Un procedimiento para producir la proteina apoacuorina Expired ES8705467A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US68790384A 1984-12-31 1984-12-31
US70230885A 1985-02-15 1985-02-15

Publications (2)

Publication Number Publication Date
ES550528A0 ES550528A0 (es) 1987-05-01
ES8705467A1 true ES8705467A1 (es) 1987-05-01

Family

ID=27104107

Family Applications (1)

Application Number Title Priority Date Filing Date
ES550528A Expired ES8705467A1 (es) 1984-12-31 1985-12-30 Un procedimiento para producir la proteina apoacuorina

Country Status (12)

Country Link
US (1) US5162227A (es)
EP (2) EP0540064B1 (es)
JP (2) JP2894687B2 (es)
CN (1) CN1010414B (es)
AT (2) ATE159291T1 (es)
CA (1) CA1274481A (es)
DE (2) DE3588168T2 (es)
DK (1) DK604385A (es)
ES (1) ES8705467A1 (es)
FI (1) FI855141L (es)
NO (1) NO855223L (es)
NZ (1) NZ214563A (es)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5422266A (en) * 1984-12-31 1995-06-06 University Of Georgia Research Foundation, Inc. Recombinant DNA vectors capable of expressing apoaequorin
JPS62171695A (ja) * 1985-12-14 1987-07-28 Chisso Corp 発光蛋白アクアリン遺伝子を用いるアクアリン活性を有する蛋白質の製造法
JPS62261942A (ja) * 1986-05-08 1987-11-14 Chisso Corp 金属の検出方法
US5093240A (en) * 1986-10-15 1992-03-03 Chisso Corporation Variant aequorin genes and process for producing variant aequorin proteins
JPS63102695A (ja) * 1986-10-20 1988-05-07 Chisso Corp 大腸菌の菌体外分泌ベクタ−を用いる発光蛋白エクオリンの製造法
JPS6439990A (en) * 1987-08-05 1989-02-10 Chisso Corp Fused gene by bonding aequorin gene to functional gene
JPS6447379A (en) * 1987-08-19 1989-02-21 Chisso Corp Preparation of calcium-dependent oxygenation enzyme
JP2592623B2 (ja) * 1987-11-18 1997-03-19 チッソ株式会社 アポエクオリンの製造法
AU3342689A (en) * 1988-03-24 1989-10-16 Igen Incorporated Luminescent chimeric proteins
JPH01285196A (ja) * 1988-05-11 1989-11-16 Chisso Corp 発光蛋白エクオリンの製造法
US5292658A (en) * 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US5360728A (en) * 1992-12-01 1994-11-01 Woods Hole Oceanographic Institution (W.H.O.I.) Modified apoaequorin having increased bioluminescent activity
JP3810791B2 (ja) * 1993-09-10 2006-08-16 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク 緑色蛍光タンパク質の使用
WO1995021191A1 (en) * 1994-02-04 1995-08-10 William Ward Bioluminescent indicator based upon the expression of a gene for a modified green-fluorescent protein
US6329139B1 (en) 1995-04-25 2001-12-11 Discovery Partners International Automated sorting system for matrices with memory
US5804387A (en) * 1996-02-01 1998-09-08 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
DE69732667T2 (de) 1996-12-12 2006-01-19 Prolume, Ltd., Beverly Hills Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen
US5876935A (en) * 1997-01-08 1999-03-02 Dade Behring Inc. Luminescent specific binding assay
US6090919A (en) * 1997-01-31 2000-07-18 The Board Of Trustees Of The Leland Stanford Junior University FACS-optimized mutants of the green fluorescent protein (GFP)
AU741974B2 (en) 1997-04-04 2001-12-13 Board Of Regents Of The University Of Nebraska, The Methods and materials for making and using transgenic dicamba-degrading organisms
US6284496B1 (en) 1997-10-03 2001-09-04 University Of South Florida DNA vector for determining the presence of out-of-reading-frame mutations
US6248542B1 (en) * 1997-12-09 2001-06-19 Massachusetts Institute Of Technology Optoelectronic sensor
EP1064360B1 (en) 1998-03-27 2008-03-05 Prolume, Ltd. Luciferases, gfp fluorescent proteins, their nucleic acids and the use thereof in diagnostics
EP1925320A3 (en) 1998-03-27 2008-09-03 Prolume, Ltd. Luciferases, fluorescent proteins, nucleic acids encoding the luciferases and fluorescent proteins and the use thereof in diagnostics
EP1031630B1 (en) * 1999-02-22 2004-10-20 Matsushita Electric Industrial Co., Ltd. Method for detecting bacteria
US7109315B2 (en) 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
US8216797B2 (en) * 2001-02-07 2012-07-10 Massachusetts Institute Of Technology Pathogen detection biosensor
US7422860B2 (en) 2001-02-07 2008-09-09 Massachusetts Institute Of Technology Optoelectronic detection system
ATE506452T1 (de) 2001-02-07 2011-05-15 Massachusetts Inst Technology Optoelektronisches detektionssystem
FR2836475B1 (fr) 2002-02-26 2007-02-23 Aventis Pharma Sa Procede de detection de ligands du recepteur de la leptine
US7663022B1 (en) 2002-07-15 2010-02-16 Bruce Eric Hudkins Transgenic bioluminescent plants
US7049483B1 (en) 2002-07-15 2006-05-23 Bruce Eric Hudkins Transgenic bioluminescent plants
US7345160B2 (en) 2004-03-29 2008-03-18 University Of Kentucky Research Foundation Aequorin and obelin mutants with differing wavelengths and bioluminescence
EP2454279A1 (en) * 2009-07-14 2012-05-23 EMD Millipore Corporation Modified photoproteins with increased affinity for calcium and enhanced bioluminescence and uses thereof
MA40959A (fr) * 2014-11-11 2017-09-19 Quincy Bioscience Llc Compositions contenant de l'apoaéquorine et leurs procédés d'utilisation pour traiter l'inflammation neuronale

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4604364A (en) * 1974-01-04 1986-08-05 Kosak Kenneth M Bioluminescent tracer composition and method of use in immunoassays
US4396579A (en) * 1981-08-06 1983-08-02 Miles Laboratories, Inc. Luminescence detection device
JPS61135586A (ja) * 1984-08-24 1986-06-23 Chisso Corp 発光蛋白アクアリン遺伝子を有するプラスミドおよび該発光蛋白の生合成法
JPS62171695A (ja) * 1985-12-14 1987-07-28 Chisso Corp 発光蛋白アクアリン遺伝子を用いるアクアリン活性を有する蛋白質の製造法

Also Published As

Publication number Publication date
CN1010414B (zh) 1990-11-14
EP0540064A1 (en) 1993-05-05
JP2655981B2 (ja) 1997-09-24
NO855223L (no) 1986-07-01
ATE93867T1 (de) 1993-09-15
FI855141A7 (fi) 1986-07-01
JPS61224989A (ja) 1986-10-06
EP0187519B1 (en) 1993-09-01
CN85109740A (zh) 1987-02-04
AU5142085A (en) 1986-07-10
FI855141L (fi) 1986-07-01
JPH06217779A (ja) 1994-08-09
ES550528A0 (es) 1987-05-01
DK604385A (da) 1986-07-01
FI855141A0 (fi) 1985-12-23
CA1274481A (en) 1990-09-25
DE3588168T2 (de) 1998-04-23
DE3588168D1 (de) 1997-11-20
NZ214563A (en) 1991-05-28
DE3587558D1 (de) 1993-10-07
DE3587558T2 (de) 1994-03-31
EP0540064B1 (en) 1997-10-15
JP2894687B2 (ja) 1999-05-24
EP0187519A2 (en) 1986-07-16
EP0187519A3 (en) 1988-01-27
US5162227A (en) 1992-11-10
AU589930B2 (en) 1989-10-26
ATE159291T1 (de) 1997-11-15
DK604385D0 (da) 1985-12-23

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20070129