ES8700881A1 - Reagent for assaying glucose, cholesterol, uric acid and haemoglobin - Google Patents
Reagent for assaying glucose, cholesterol, uric acid and haemoglobinInfo
- Publication number
- ES8700881A1 ES8700881A1 ES544552A ES544552A ES8700881A1 ES 8700881 A1 ES8700881 A1 ES 8700881A1 ES 544552 A ES544552 A ES 544552A ES 544552 A ES544552 A ES 544552A ES 8700881 A1 ES8700881 A1 ES 8700881A1
- Authority
- ES
- Spain
- Prior art keywords
- esp
- units
- reagent
- pref
- cholesterol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 title abstract 2
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title abstract 2
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title abstract 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 title abstract 2
- 235000012000 cholesterol Nutrition 0.000 title abstract 2
- 239000008103 glucose Substances 0.000 title abstract 2
- 229940116269 uric acid Drugs 0.000 title abstract 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- 102000003992 Peroxidases Human genes 0.000 abstract 3
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical group [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000003599 detergent Substances 0.000 abstract 2
- 229940088598 enzyme Drugs 0.000 abstract 2
- 239000003381 stabilizer Substances 0.000 abstract 2
- MGSRCZKZVOBKFT-UHFFFAOYSA-N thymol Chemical compound CC(C)C1=CC=C(C)C=C1O MGSRCZKZVOBKFT-UHFFFAOYSA-N 0.000 abstract 2
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N 4-aminoantipyrine Chemical compound CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 abstract 1
- 108010089254 Cholesterol oxidase Proteins 0.000 abstract 1
- 108010015776 Glucose oxidase Proteins 0.000 abstract 1
- 239000004366 Glucose oxidase Substances 0.000 abstract 1
- 239000007836 KH2PO4 Substances 0.000 abstract 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 abstract 1
- 239000005844 Thymol Substances 0.000 abstract 1
- 108010092464 Urate Oxidase Proteins 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 229940116332 glucose oxidase Drugs 0.000 abstract 1
- 235000019420 glucose oxidase Nutrition 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 229940051841 polyoxyethylene ether Drugs 0.000 abstract 1
- 229920000056 polyoxyethylene ether Polymers 0.000 abstract 1
- 229960000790 thymol Drugs 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Reagent for determining glucose, cholesterol, uric acid or haemoglobin comprises (1) 1-phenyl-2,3-dimethyl -4-aminopyrazol-5-one (I); (2) a phenol deriv.; (3) buffer soln.; (4) at least 1 stabiliser, and opt. (5) at least one detergent and/or (6) at least one enzyme catalyst. The new feature is that component (2) is thymol (II). Pref. (II) is present as a soln. in 1-4C alcohol, esp. EtOH. - (II) is 2.3-2.7, esp. 2.5 mM; (I) 0.6-1, esp. 0.8 mM and the buffer is aq. Na2HPO4-KH2PO4 at 0.06-0.1, esp. 0.08 M. The pref. stabiliser is NaN3 at 9.75-9.95, esp. 9.85 mM and the detergents are polyoxyethylene ether and/or polyalkanolalkyl ether at 0.2-1, esp. 0.4 wt.%. - The pref. enzymes are (A) glucose oxidase at 8-12, esp. 10, units/ml plus peroxidase at 13-17, esp. 15, units/ml; (B) cholesterinase at 8-12, esp. 10, units/ml; cholesterol oxidase at 4-6, esp. 5, units/ml and peroxidase as in A and (c) uricase at 4-6, esp. 5, units/ml and peroxidase as in A. The reagent pref. contains 310-350, esp. 330, mM alkanol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES544552A ES8700881A1 (en) | 1985-06-25 | 1985-06-25 | Reagent for assaying glucose, cholesterol, uric acid and haemoglobin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES544552A ES8700881A1 (en) | 1985-06-25 | 1985-06-25 | Reagent for assaying glucose, cholesterol, uric acid and haemoglobin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8700881A1 true ES8700881A1 (en) | 1986-10-16 |
| ES544552A0 ES544552A0 (en) | 1986-10-16 |
Family
ID=8489421
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES544552A Expired ES8700881A1 (en) | 1985-06-25 | 1985-06-25 | Reagent for assaying glucose, cholesterol, uric acid and haemoglobin |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES8700881A1 (en) |
-
1985
- 1985-06-25 ES ES544552A patent/ES8700881A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES544552A0 (en) | 1986-10-16 |
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