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ES8608579A1 - Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular - Google Patents

Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular

Info

Publication number
ES8608579A1
ES8608579A1 ES534102A ES534102A ES8608579A1 ES 8608579 A1 ES8608579 A1 ES 8608579A1 ES 534102 A ES534102 A ES 534102A ES 534102 A ES534102 A ES 534102A ES 8608579 A1 ES8608579 A1 ES 8608579A1
Authority
ES
Spain
Prior art keywords
industrial
strains
expression
interest
molecular cloning
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES534102A
Other languages
English (en)
Other versions
ES534102A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Gist Brocades NV
Original Assignee
Gist Brocades NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8190976&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8608579(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Gist Brocades NV filed Critical Gist Brocades NV
Publication of ES8608579A1 publication Critical patent/ES8608579A1/es
Publication of ES534102A0 publication Critical patent/ES534102A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Compounds Of Unknown Constitution (AREA)

Abstract

METODO PARA LA INTRODUCCION DE DNA EN UN ANFITRION DE CAPA DE MICROORGANISMO UNICELULAR, CAPAZ DE MANTENER Y EXPRESAR DE MANERA ESTABLE UN POLIPEPTIDO EN DICHO ANFITRION. COMPRENDE LA COMBINACION, EN PRESENCIA DE UN FUSOGENO, DE PRIMERAS CELULAS ANFITRIONES FACILMENTE TRANSFORMABLES QUE CONTIENEN EL DNA COMO UN ELEMENTO EXTRACROMOSOMAL CAPAZ DE MANTENER EN EL PRIMER ANFITRION, CON CELULAS ANFITRIONES DE CEPA DE MICROORGANISMO UNICELULAR, INDUSTRIAL, RESISTENTE A LA MODIFICACION GENETICA POR UN VECTOR, PLASMIDO, FAGO O COSMIDO, EN CONDICIONES TALES QUE SE PROPORCIONE LA SELECCION DE LAS CELULAS DEL ANFITRION DE CEPA CON LO QUE SE TRANSFIERE A LAS MISMAS EL DNA; Y SELECCION DE LAS CELULAS DEL ANFITRION DE CEPA DE MICROORGANISMO UNICELULAR QUE CONTIENEN DICHO DNA. TIENE APLICACIONES PARA LA PRODUCCION DE POLIPEPTIDOS Y MATERIALES PROTEINICOS ENDOGENOS Y EXOGENOS.
ES534102A 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular Expired ES8608579A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP83201016 1983-07-06

Publications (2)

Publication Number Publication Date
ES8608579A1 true ES8608579A1 (es) 1986-06-16
ES534102A0 ES534102A0 (es) 1986-06-16

Family

ID=8190976

Family Applications (2)

Application Number Title Priority Date Filing Date
ES534102A Expired ES8608579A1 (es) 1983-07-06 1984-07-06 Procedimiento para introducir dna en un anfitrion de cepa de microorganismo unicelular
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES545256A Expired ES8609467A1 (es) 1983-07-06 1985-07-16 Procedimiento para preparar un polipetido de interes en una cepa de bacilus industrial

Country Status (11)

Country Link
EP (1) EP0134048B2 (es)
JP (2) JP2669457B2 (es)
AU (1) AU570709B2 (es)
DE (1) DE3480411D1 (es)
DK (1) DK169996B1 (es)
ES (2) ES8608579A1 (es)
FI (1) FI85287C (es)
GR (1) GR82200B (es)
IE (1) IE58014B1 (es)
NZ (1) NZ208806A (es)
WO (1) WO1985000382A1 (es)

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JPS60188093A (ja) * 1984-03-09 1985-09-25 Teruhiko Beppu 枯草菌に於ける形質発現プラスミド
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ZA81424B (en) * 1980-02-15 1982-02-24 Cpc International Inc Genetically engineered microorganisms for massive production of amylolytic enzymes and process for preparing same
AU545912B2 (en) * 1980-03-10 1985-08-08 Cetus Corporation Cloned heterologous jive products in bacillies
JPS56137896A (en) * 1980-03-10 1981-10-28 Cetus Corp Genetic recombination gram positive bacteria , production thereof and protein for mammal produced by said gram positive bacteria
FI64813C (fi) * 1980-12-31 1984-01-10 Ilkka Antero Palva Foerfarande foer producering av ett utvalt aeggviteaemne och vid foerfarandet anvaenda rekombinantplasmidvektorer
CA1206108A (en) * 1981-03-06 1986-06-17 Shuichi Aiba Process for transforming microorganism by means of plasmid introduction
BR8205954A (pt) * 1981-10-14 1983-09-13 Unilever Nv Sequencia de dna,plasmidio recombinante,cultura bacteriana e microorganismos
JPS5889194A (ja) * 1981-11-24 1983-05-27 Ajinomoto Co Inc 微生物によるｌ−トリプトフアンの製造法
JPS58107192A (ja) * 1981-12-18 1983-06-25 Ajinomoto Co Inc 発酵法によるｌ−ヒスチジンの製造法

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JPH06189783A (ja) 1994-07-12
ES545256A0 (es) 1986-08-01
DE3480411D1 (en) 1989-12-14
FI85287B (fi) 1991-12-13
GR82200B (es) 1984-12-13
AU3151084A (en) 1985-02-07
DK169996B1 (da) 1995-04-24
JPS60501738A (ja) 1985-10-17
IE841726L (en) 1985-01-06
IE58014B1 (en) 1993-06-16
ES534102A0 (es) 1986-06-16
EP0134048B1 (en) 1989-11-08
DK101385D0 (da) 1985-03-05
FI850845A0 (fi) 1985-03-01
JP2669457B2 (ja) 1997-10-27
NZ208806A (en) 1988-07-28
EP0134048A1 (en) 1985-03-13
FI850845L (fi) 1985-03-01
EP0134048B2 (en) 1996-08-14
FI85287C (fi) 1992-03-25
ES8609467A1 (es) 1986-08-01
WO1985000382A1 (en) 1985-01-31
DK101385A (da) 1985-03-05
JP2664860B2 (ja) 1997-10-22
AU570709B2 (en) 1988-03-24

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