ES8608041A1 - Tissue culture and production in permeable gels - comprises proliferating or preserving mammalian, e.g. hybridoma, cells by trapping in gelled alkali alginate droplets - Google Patents
Tissue culture and production in permeable gels - comprises proliferating or preserving mammalian, e.g. hybridoma, cells by trapping in gelled alkali alginate dropletsInfo
- Publication number
- ES8608041A1 ES8608041A1 ES543705A ES543705A ES8608041A1 ES 8608041 A1 ES8608041 A1 ES 8608041A1 ES 543705 A ES543705 A ES 543705A ES 543705 A ES543705 A ES 543705A ES 8608041 A1 ES8608041 A1 ES 8608041A1
- Authority
- ES
- Spain
- Prior art keywords
- cells
- hybridoma
- trapping
- production
- tissue culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 210000004027 cell Anatomy 0.000 title abstract 5
- FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical compound O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 title abstract 2
- 229940072056 alginate Drugs 0.000 title abstract 2
- 235000010443 alginic acid Nutrition 0.000 title abstract 2
- 229920000615 alginic acid Polymers 0.000 title abstract 2
- 239000003513 alkali Substances 0.000 title abstract 2
- 239000000499 gel Substances 0.000 title 1
- 210000004408 hybridoma Anatomy 0.000 title 1
- 230000002062 proliferating effect Effects 0.000 title 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 238000012423 maintenance Methods 0.000 abstract 1
- 210000004962 mammalian cell Anatomy 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 239000000725 suspension Substances 0.000 abstract 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
Viable mammalian cells are proliferated by (1) suspending cells in alkali alginate (I) soln.; (2) forming the suspension into droplets; (3) gelling these droplets to form shape-retaining structures around the cells; (4) transferring these structures to a proliferation-promoting growth medium and (5) growing these cells within the structures. Alternatively the structures are transferred to a maintenance medium to preserve the cells.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US61653484A | 1984-06-01 | 1984-06-01 | |
| PCT/US1985/000838 WO1985005630A1 (en) | 1984-06-01 | 1985-05-08 | Tissue culture and production in permeable gels |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES8608041A1 true ES8608041A1 (en) | 1986-06-01 |
| ES543705A0 ES543705A0 (en) | 1986-06-01 |
Family
ID=26771821
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES543705A Expired ES8608041A1 (en) | 1984-06-01 | 1985-05-30 | Tissue culture and production in permeable gels - comprises proliferating or preserving mammalian, e.g. hybridoma, cells by trapping in gelled alkali alginate droplets |
Country Status (2)
| Country | Link |
|---|---|
| CA (1) | CA1271438A (en) |
| ES (1) | ES8608041A1 (en) |
-
1985
- 1985-05-29 CA CA000482745A patent/CA1271438A/en not_active Expired - Fee Related
- 1985-05-30 ES ES543705A patent/ES8608041A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| ES543705A0 (en) | 1986-06-01 |
| CA1271438A (en) | 1990-07-10 |
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