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ES8602039A1 - Procedimiento para producir microsoportes de cultivos de ce-lulas - Google Patents

Procedimiento para producir microsoportes de cultivos de ce-lulas

Info

Publication number
ES8602039A1
ES8602039A1 ES539867A ES539867A ES8602039A1 ES 8602039 A1 ES8602039 A1 ES 8602039A1 ES 539867 A ES539867 A ES 539867A ES 539867 A ES539867 A ES 539867A ES 8602039 A1 ES8602039 A1 ES 8602039A1
Authority
ES
Spain
Prior art keywords
preparing
gps
cell culture
dependent cells
crosslinked
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES539867A
Other languages
English (en)
Other versions
ES539867A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfeifer and Langen GmbH and Co KG
Original Assignee
Pfeifer and Langen GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfeifer and Langen GmbH and Co KG filed Critical Pfeifer and Langen GmbH and Co KG
Publication of ES8602039A1 publication Critical patent/ES8602039A1/es
Publication of ES539867A0 publication Critical patent/ES539867A0/es
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
    • C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
    • C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C12N5/0075—General culture methods using substrates using microcarriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Materials Engineering (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Cephalosporin Compounds (AREA)

Abstract

PROCEDIMIENTO PARA PRODUCIR MICROSOPORTES DE CULTIVOS DE CELULAS. SE REALIZA UNA SUSPENSION DE UN POLISACARIDO RETICULADO EN UNA DISOLUCION ACUOSA-ALCALINA QUE SE VIERTE EN TOLUENO MODERADAMENTE CALIENTE Y SE AGITA PARA HACERLA REACCIONAR CON UN COMPUESTO (II) DONDE Z ES UNA CADENA HIDROCARBONADA DE AL MENOS DOS ATOMOS DE CARBONO, R1 Y R2 SON IGUALES O DISTINTOS Y REPRESENTAN GRUPOS ALQUILO, ARILO O ARALQUILO E Y ES UN GRUPO REACTIVO. ESTA REACCION SE EFECTUA EN PRESENCIA DE BASES FUERTES, EN DOS ETAPAS, AL MENOS, Y CON, POR LO MENOS, UN EXCESO DE 2 VECES SOBRE LA CANTIDAD EQUIMOLAR DEL COMPUESTO (II). FINALMENTE SE OBTIENEN LOS MICROSOPORTES, CON PORCIONES QUIMICAS CARGADAS POSITIVAMENTE, QUE CORRESPONDEN A LA FORMULA GENERAL (I).
ES539867A 1984-01-28 1985-01-25 Procedimiento para producir microsoportes de cultivos de ce-lulas Granted ES539867A0 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19843402927 DE3402927A1 (de) 1984-01-28 1984-01-28 Zellkulturmikrotraeger, verfahren zu seiner herstellung und seine verwendung zum zuechten von verankerungsabhaengigen zellen

Publications (2)

Publication Number Publication Date
ES8602039A1 true ES8602039A1 (es) 1985-11-16
ES539867A0 ES539867A0 (es) 1985-11-16

Family

ID=6226136

Family Applications (1)

Application Number Title Priority Date Filing Date
ES539867A Granted ES539867A0 (es) 1984-01-28 1985-01-25 Procedimiento para producir microsoportes de cultivos de ce-lulas

Country Status (10)

Country Link
US (2) US4824946A (es)
EP (1) EP0150796B1 (es)
JP (1) JPS60237991A (es)
AT (1) ATE46717T1 (es)
CA (1) CA1269628A (es)
DE (2) DE3402927A1 (es)
DK (1) DK37985A (es)
ES (1) ES539867A0 (es)
IE (1) IE850175L (es)
PT (1) PT79884B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6410979A (en) * 1987-07-03 1989-01-13 Mitsubishi Chem Ind Microcarrier for cultivating cell
KR920700232A (ko) * 1988-06-03 1992-02-19 원본미기재 글리코사미노글리칸 염류, 그 제조방법 및 이를 함유하는 제약 조성물
US5349089A (en) 1989-07-07 1994-09-20 National Starch And Chemical Investment Holding Corporation Reagent for preparing polycationic polysaccharides
US5227481A (en) * 1989-07-07 1993-07-13 National Starch And Chemical Investment Holding Corporation Cationic polysaccharides and reagents for their preparation
ATE175242T1 (de) * 1991-02-28 1999-01-15 Anticancer Inc Ein gewebekulturverfahren für haut im natürlichen zustand
DE19954357C2 (de) * 1999-11-11 2002-09-05 Cosmedica Consulting Gmbh Zusammensetzung, enthaltend Heteropolysaccharid und perlenförmiges quervernetztes Polysaccharid, sowie deren Verwendung für medizinische/kosmetische Zwecke
FR2836923B1 (fr) * 2002-02-25 2007-06-15 Pentax Corp Support pour culture de cellules et procede pour cultiver des cellules.
US20080020049A1 (en) * 2005-02-25 2008-01-24 Andrew Darling Super-sparger microcarrier beads and precision extrusion deposited poly-epsilon-caprolactone structures for biological applications
US20090047260A1 (en) * 2007-08-17 2009-02-19 Wake Forest University Health Sciences Keratin biomaterials for cell culture and methods of use
WO2015042125A2 (en) 2013-09-19 2015-03-26 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for producing hepatocyte-like cells

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3910819A (en) * 1974-02-19 1975-10-07 California Inst Of Techn Treatment of surfaces to stimulate biological cell adhesion and growth
JPS6026121B2 (ja) * 1977-02-28 1985-06-21 名糖産業株式会社 新規デキストラン誘導体及びその製法
FR2400063A1 (fr) * 1977-08-08 1979-03-09 Pasteur Institut Procede d'obtention de supports pour cultures cellulaires et supports obtenus
US4293654A (en) * 1977-10-17 1981-10-06 Massachusetts Institute Of Technology Cell culture microcarriers
SE445116B (sv) * 1979-09-12 1986-06-02 Pharmacia Fine Chemicals Ab Sett att odla celler pa mikroberare med fibronektinytskikt
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
US4415668A (en) * 1981-04-20 1983-11-15 President And Fellows Of Harvard College Cell culture
SE8103137L (sv) * 1981-05-19 1982-11-20 Pharmacia Ab Polymer med kvartera aminogrupper
SE8103138L (sv) * 1981-05-19 1982-11-20 Pharmacia Fine Chemicals Ab Mikroberare for odling av forankringsberoende celler
IL74259A0 (en) * 1984-02-06 1985-05-31 Surface Concepts Pty Ltd Improved method for cell culture
SE454518B (sv) * 1984-05-21 1988-05-09 Statens Bakteriologiska Lab Forfarande for odling av diploida celler i nervaro av cellulosafibrer
US4661407A (en) * 1985-01-07 1987-04-28 Kms Fusion, Inc. Glass-surface microcarrier for anchorage-dependent cell cultivation
KR950006247B1 (ko) * 1986-02-28 1995-06-12 소니 가부시끼가이샤 시간축 신장 재생장치
JPS63237782A (ja) * 1987-03-27 1988-10-04 Teijin Ltd 付着性動物細胞の培養方法

Also Published As

Publication number Publication date
PT79884B (en) 1986-10-23
EP0150796A3 (en) 1988-01-07
ATE46717T1 (de) 1989-10-15
US4824946A (en) 1989-04-25
US4910142A (en) 1990-03-20
PT79884A (en) 1985-02-01
ES539867A0 (es) 1985-11-16
DE3402927C2 (es) 1989-03-09
JPS60237991A (ja) 1985-11-26
DE3402927A1 (de) 1985-08-08
EP0150796B1 (de) 1989-09-27
DK37985D0 (da) 1985-01-28
DK37985A (da) 1985-07-29
CA1269628A (en) 1990-05-29
DE3573273D1 (en) 1989-11-02
IE850175L (en) 1985-07-28
EP0150796A2 (de) 1985-08-07

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Legal Events

Date Code Title Description
MM4A Patent lapsed

Effective date: 19960506