ES2684587T3 - Producción de bibliotecas químicas codificadas - Google Patents
Producción de bibliotecas químicas codificadas Download PDFInfo
- Publication number
- ES2684587T3 ES2684587T3 ES14824390T ES14824390T ES2684587T3 ES 2684587 T3 ES2684587 T3 ES 2684587T3 ES 14824390 T ES14824390 T ES 14824390T ES 14824390 T ES14824390 T ES 14824390T ES 2684587 T3 ES2684587 T3 ES 2684587T3
- Authority
- ES
- Spain
- Prior art keywords
- nucleic acid
- strands
- coupled
- strand
- chemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B20/00—Methods specially adapted for identifying library members
- C40B20/06—Methods specially adapted for identifying library members using iterative deconvolution techniques
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
- C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Plant Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Structural Engineering (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
Abstract
Método de producción de una biblioteca química codificada por ácido nucleico que comprende; (i) producir una sub-biblioteca según un método que comprende; (a) proporcionar una población de primeras hebras de ácido nucleico, estando acoplada o pudiendo acoplarse cada hebra de ácido nucleico a un miembro de una primera población diversa de restos químicos y que comprende un espaciador no hibridable, (b) poner en contacto las primeras hebras de ácido nucleico con oligonucleótidos identificadores que comprenden una primera secuencia codificante y uno o más oligonucleótidos adaptadores, de manera que el uno o más oligonucleótidos adaptadores se hibridan con las hebras de ácido nucleico y los oligonucleótidos identificadores para formar un complejo parcialmente bicatenario, en el que cada primera hebra de ácido nucleico se pone en contacto con un oligonucleótido identificador que comprende una primera secuencia codificante que codifica para un resto químico que está acoplado o puede acoplarse a la primera hebra de ácido nucleico, y; en el que cada uno de dicho uno o más oligonucleótidos adaptadores se hibrida con más de una primera hebra de ácido nucleico en la población y más de un oligonucleótido identificador diferente, (c) ligar las primeras hebras de ácido nucleico con los oligonucleótidos identificadores en los complejos parcialmente bicatenarios, de manera que los oligonucleótidos identificadores se incorporan en las primeras hebras de ácido nucleico, produciendo de ese modo una sub-biblioteca que comprende primeras hebras de ácido nucleico acopladas o que pueden acoplarse a un miembro de una población diversa de restos químicos, en el que cada primera hebra de ácido nucleico comprende una primera secuencia codificante que codifica para el resto químico que está acoplado a la primera hebra de ácido nucleico y el espaciador no hibridable; (ii) hibridar las primeras hebras de ácido nucleico con segundas hebras de ácido nucleico para formar complejos bicatenarios, en el que las segundas hebras de ácido nucleico se acoplan con una segunda población diversa de restos químicos, comprendiendo cada segunda hebra de ácido nucleico una segunda secuencia codificante que codifica para el resto químico que está acoplado a la misma, correspondiendo la posición de la segunda secuencia codificante en las segundas hebras de ácido nucleico en el complejo bicatenario a la posición del espaciador en las primeras hebras de ácido nucleico en los complejos bicatenarios, de manera que las segundas secuencias codificantes no se hibridan preferentemente con las primeras hebras de ácido nucleico, y (iii) extender las segundas hebras de ácido nucleico a lo largo de las hebras de ácido nucleico para producir una biblioteca que comprende miembros que tienen una molécula de ácido nucleico bicatenario que comprende las hebras de ácido nucleico primera y segunda; estando acoplada la primera población diversa de restos químicos a las primeras hebras de ácido nucleico y estando acoplada la segunda población diversa de restos químicos a las segundas hebras de ácido nucleico, dichos restos químicos forman farmacóforos en los miembros de la biblioteca, en el que cada segunda hebra de ácido nucleico comprende secuencias codificantes primera y segunda que codifican para los restos químicos de las poblaciones diversas primera y segunda.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1322692.3A GB201322692D0 (en) | 2013-12-20 | 2013-12-20 | Production of encoded chemical libraries |
| PCT/EP2014/077403 WO2015091207A1 (en) | 2013-12-20 | 2014-12-11 | Production of encoded chemical libraries |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2684587T3 true ES2684587T3 (es) | 2018-10-03 |
Family
ID=50071234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES14824390T Active ES2684587T3 (es) | 2013-12-20 | 2014-12-11 | Producción de bibliotecas químicas codificadas |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10240147B2 (es) |
| EP (1) | EP3083957B1 (es) |
| AU (1) | AU2014365162B2 (es) |
| CA (1) | CA2934126C (es) |
| ES (1) | ES2684587T3 (es) |
| GB (1) | GB201322692D0 (es) |
| WO (1) | WO2015091207A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010094036A1 (en) | 2009-02-13 | 2010-08-19 | X-Chem, Inc. | Methods of creating and screening dna-encoded libraries |
| SG10201605812YA (en) | 2011-09-07 | 2016-09-29 | Chem Inc X | Methods for tagging dna-encoded libraries |
| KR102146721B1 (ko) * | 2012-07-13 | 2020-08-21 | 엑스-켐, 인크. | 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리 |
| GB201322692D0 (en) | 2013-12-20 | 2014-02-05 | Philochem Ag | Production of encoded chemical libraries |
| GB201603789D0 (en) | 2016-03-04 | 2016-04-20 | Apta Biosciences Ltd | Oligonucleotides and methods for preparing |
| WO2017214067A1 (en) * | 2016-06-05 | 2017-12-14 | Dice Molecules Sv, Llc | Methods of routing, compositions and uses thereof |
| WO2017218293A1 (en) | 2016-06-16 | 2017-12-21 | Richard Edward Watts | Oligonucleotide directed and recorded combinatorial synthesis of encoded probe molecules |
| US20200190507A1 (en) * | 2016-11-10 | 2020-06-18 | The Scripps Research Institute | Encoded Solid Phase Compound Library with Polynucleotide Based Barcoding |
| GB201622222D0 (en) | 2016-12-23 | 2017-02-08 | Cs Genetics Ltd | Reagents and methods for molecular barcoding of nucleic acids of single cells |
| CN110325491B (zh) * | 2017-03-17 | 2022-04-19 | 成都先导药物开发股份有限公司 | 编码库的合成方法及组合物 |
| WO2018204420A1 (en) | 2017-05-02 | 2018-11-08 | Haystack Sciences Corporation | Molecules for verifying oligonucleotide directed combinatorial synthesis and methods of making and using the same |
| WO2020092614A1 (en) * | 2018-10-31 | 2020-05-07 | Rutgers, The State University Of New Jersey | Gramc: genome-scale reporter assay method for cis-regulatory modules |
| GB201821109D0 (en) | 2018-12-21 | 2019-02-06 | Philochem Ag | Nucleic acid encoded chemical libraries |
| CN110346493B (zh) * | 2019-05-23 | 2021-01-05 | 中国人民解放军军事科学院军事医学研究院 | 检测猕猴血浆中中期因子反义寡核苷酸含量的方法 |
| CN112575389B (zh) * | 2019-09-30 | 2023-09-19 | 成都先导药物开发股份有限公司 | 一种dna编码化合物库的液相色谱纯化方法 |
| CN114790578A (zh) * | 2021-01-26 | 2022-07-26 | 成都先导药物开发股份有限公司 | 一种dna编码化合物库的rna靶标筛选方法 |
| WO2024069235A2 (en) | 2022-09-30 | 2024-04-04 | Sixfold Bioscience Ltd. | Compositions containing oligonucleotides with theranostic applications |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT971946E (pt) | 1997-01-21 | 2006-11-30 | Gen Hospital Corp | Selecção de proteínas utilizando fusões arn-proteína |
| US6261804B1 (en) | 1997-01-21 | 2001-07-17 | The General Hospital Corporation | Selection of proteins using RNA-protein fusions |
| US20030144489A1 (en) | 1997-06-09 | 2003-07-31 | Alex Burgin | Method for screening nucleic acid catalysts |
| JP4128453B2 (ja) | 2001-03-19 | 2008-07-30 | プレジデント アンド フェロウズ オブ ハーバード カレッジ | 新規分子機能の進化 |
| JP2004535193A (ja) | 2001-06-20 | 2004-11-25 | ヌエヴォリューション・アクティーゼルスカブ | 鋳型化された分子およびかかる分子の使用方法 |
| KR100916889B1 (ko) * | 2002-03-08 | 2009-09-09 | 아이드게노쉬쉐 테흐니쉐 호흐슐레 쥬리히 | 암호화된 자가-조립 화학적 라이브러리(ｅｓａｃｈｅｌ) |
| CN101006177B (zh) | 2002-03-15 | 2011-10-12 | 纽韦卢森公司 | 改善的用于合成模制分子的方法 |
| AU2003247266A1 (en) | 2002-08-01 | 2004-02-23 | Nuevolution A/S | Multi-step synthesis of templated molecules |
| CN106337046B (zh) | 2002-10-30 | 2021-03-23 | 纽韦卢森公司 | 合成双功能复合物的方法 |
| ATE450609T1 (de) | 2002-12-19 | 2009-12-15 | Nuevolution As | Durch quasizufallsstrukturen und funktionen geführte synthesemethode |
| US7915201B2 (en) | 2003-03-20 | 2011-03-29 | Nuevolution A/S | Ligational encoding of small molecules |
| ATE447626T1 (de) | 2003-09-18 | 2009-11-15 | Nuevolution As | Methode zur gewinnung struktureller informationen kodierter moleküle und zur selektion von verbindungen |
| JP5646127B2 (ja) | 2003-12-17 | 2014-12-24 | グラクソスミスクライン・リミテッド・ライアビリティ・カンパニーＧｌａｘｏＳｍｉｔｈＫｌｉｎｅ ＬＬＣ | コードされたライブラリーの合成のための方法 |
| AU2005300886B2 (en) | 2004-11-08 | 2009-11-12 | Vipergen Aps | Structural nucleic acid guided chemical synthesis |
| LT3018206T (lt) | 2005-12-01 | 2021-12-10 | Nuevolution A/S | Fermentiniai kodavimo būdai, skirti didelių bibliotekų efektyviai sintezei |
| US7928211B2 (en) | 2006-05-03 | 2011-04-19 | Vipergen Pharmaceuticals Aps | Method for preparing compounds by nucleic acid directed synthesis |
| WO2009077173A2 (en) | 2007-12-19 | 2009-06-25 | Philochem Ag | Dna-encoded chemical libraries |
| WO2010094036A1 (en) * | 2009-02-13 | 2010-08-19 | X-Chem, Inc. | Methods of creating and screening dna-encoded libraries |
| CA2832672A1 (en) | 2010-04-16 | 2011-10-20 | Nuevolution A/S | Bi-functional complexes and methods for making and using such complexes |
| SG10201605812YA (en) | 2011-09-07 | 2016-09-29 | Chem Inc X | Methods for tagging dna-encoded libraries |
| KR102146721B1 (ko) | 2012-07-13 | 2020-08-21 | 엑스-켐, 인크. | 폴리머라제에 의해 판독가능하지 않은 코딩 올리고뉴클레오티드 연결을 갖는 dna-코딩된 라이브러리 |
| GB201322692D0 (en) | 2013-12-20 | 2014-02-05 | Philochem Ag | Production of encoded chemical libraries |
-
2013
- 2013-12-20 GB GBGB1322692.3A patent/GB201322692D0/en not_active Ceased
-
2014
- 2014-12-11 CA CA2934126A patent/CA2934126C/en active Active
- 2014-12-11 US US15/106,795 patent/US10240147B2/en active Active
- 2014-12-11 ES ES14824390T patent/ES2684587T3/es active Active
- 2014-12-11 WO PCT/EP2014/077403 patent/WO2015091207A1/en not_active Ceased
- 2014-12-11 EP EP14824390.0A patent/EP3083957B1/en active Active
- 2014-12-11 AU AU2014365162A patent/AU2014365162B2/en active Active
-
2019
- 2019-03-26 US US16/365,437 patent/US20200123534A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2934126A1 (en) | 2015-06-25 |
| EP3083957B1 (en) | 2018-05-23 |
| US20170009226A1 (en) | 2017-01-12 |
| AU2014365162A1 (en) | 2016-07-21 |
| WO2015091207A1 (en) | 2015-06-25 |
| AU2014365162B2 (en) | 2018-03-08 |
| CA2934126C (en) | 2019-04-23 |
| GB201322692D0 (en) | 2014-02-05 |
| US10240147B2 (en) | 2019-03-26 |
| US20200123534A1 (en) | 2020-04-23 |
| EP3083957A1 (en) | 2016-10-26 |
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