ES2676895T3 - Variantes combinatorias de alfa-amilasa - Google Patents
Variantes combinatorias de alfa-amilasa Download PDFInfo
- Publication number
- ES2676895T3 ES2676895T3 ES14722824T ES14722824T ES2676895T3 ES 2676895 T3 ES2676895 T3 ES 2676895T3 ES 14722824 T ES14722824 T ES 14722824T ES 14722824 T ES14722824 T ES 14722824T ES 2676895 T3 ES2676895 T3 ES 2676895T3
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- seq
- amino acid
- amylase
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- mutation
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Links
- 102000004139 alpha-Amylases Human genes 0.000 title abstract 8
- 108090000637 alpha-Amylases Proteins 0.000 title abstract 8
- 229940024171 alpha-amylase Drugs 0.000 title abstract 8
- 230000035772 mutation Effects 0.000 abstract 7
- 125000000539 amino acid group Chemical group 0.000 abstract 6
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 229920002472 Starch Polymers 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 235000019698 starch Nutrition 0.000 abstract 1
- 239000008107 starch Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Variante recombinante de una α-amilasa original que comprende: i) una mutación en un residuo de aminoácido E187 utilizando SEQ ID N.º 1 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N126, Y150, F153, L171, T180, y 1203 utilizando SEQ ID N.º 1 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 1; o ii) una mutación en un residuo de aminoácido E186 utilizando SEQ ID N.º 3 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N125, Y149, F152, L170, D179, y L202 utilizando SEQ ID N.º 3 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 3; o iii) una mutación en un residuo de aminoácido E189 utilizando SEQ ID N.º 5 para la numeración; y al menos una mutación en un residuo de aminoácido seleccionado del grupo que consiste en N128, Y152, F155, L173, T182, y L205 utilizando SEQ ID N.º 5 para la numeración; donde la variante de α-amilasa presenta al menos un 70 % de identidad de secuencia de aminoácidos con respecto a SEQ ID N.º 5; donde la variante presenta un aumento de la estabilidad térmica, estabilidad detergente, actividad de licuefacción de almidón, y/o rendimiento de limpieza en comparación con la α-amilasa original o una α- amilasa de referencia que difiera de la variante de α-amilasa únicamente por la ausencia de las mutaciones.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361776699P | 2013-03-11 | 2013-03-11 | |
| US201361906617P | 2013-11-20 | 2013-11-20 | |
| US201361907131P | 2013-11-21 | 2013-11-21 | |
| PCT/US2014/023458 WO2014164777A1 (en) | 2013-03-11 | 2014-03-11 | Alpha-amylase combinatorial variants |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2676895T3 true ES2676895T3 (es) | 2018-07-26 |
| ES2676895T5 ES2676895T5 (es) | 2022-04-27 |
Family
ID=50680117
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES18151284T Active ES2882517T5 (en) | 2013-03-11 | 2014-03-11 | Alpha-amylase conbinatorial variants |
| ES14722824T Active ES2676895T5 (es) | 2013-03-11 | 2014-03-11 | Variantes combinatorias de alfa-amilasa |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES18151284T Active ES2882517T5 (en) | 2013-03-11 | 2014-03-11 | Alpha-amylase conbinatorial variants |
Country Status (9)
| Country | Link |
|---|---|
| US (5) | US20160017304A1 (es) |
| EP (5) | EP2970931B1 (es) |
| CN (3) | CN105229148B9 (es) |
| BR (1) | BR112015021647B1 (es) |
| CA (1) | CA2903027A1 (es) |
| DK (3) | DK2970931T3 (es) |
| ES (2) | ES2882517T5 (es) |
| HU (1) | HUE039341T2 (es) |
| WO (3) | WO2014164834A1 (es) |
Families Citing this family (191)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK2935575T3 (en) * | 2012-12-21 | 2018-07-23 | Danisco Us Inc | ALPHA-amylase variants |
| EP3004315A2 (en) * | 2013-06-06 | 2016-04-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
| CN107835855B (zh) | 2015-05-13 | 2022-05-13 | 丹尼斯科美国公司 | AprL-进化枝蛋白酶变体及其用途 |
| WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
| WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
| ES2962329T3 (es) | 2015-06-09 | 2024-03-18 | Danisco Us Inc | Encapsulados de estallido osmótico |
| JP7015695B2 (ja) | 2015-06-17 | 2022-02-03 | ダニスコ・ユーエス・インク | バチルス・ギブソニイ（Ｂａｃｉｌｌｕｓ ｇｉｂｓｏｎｉｉ）クレードセリンプロテアーゼ |
| EP3368655B1 (en) | 2015-10-30 | 2020-06-10 | Danisco US Inc. | Enhanced protein expression and methods thereof |
| JP7364330B2 (ja) | 2015-11-05 | 2023-10-18 | ダニスコ・ユーエス・インク | パエニバチルス（Ｐａｅｎｉｂａｃｉｌｌｕｓ）属種及びバチルス（Ｂａｃｉｌｌｕｓ）属種のマンナナーゼ |
| US20190153417A1 (en) | 2015-11-05 | 2019-05-23 | Danisco Us Inc | Paenibacillus sp. mannanases |
| DK3387124T3 (da) * | 2015-12-09 | 2021-08-23 | William Cuevas | Kombinatoriske alfa-amylasevarianter |
| EP3390625B1 (en) | 2015-12-18 | 2023-09-06 | Danisco US Inc. | Polypeptides with endoglucanase activity and uses thereof |
| KR20170080537A (ko) * | 2015-12-31 | 2017-07-10 | 씨제이제일제당 (주) | 내열성 및 내산성을 가지는 Acidothermus 속 유래 탈분지 효소를 이용한 전분당으로부터 포도당의 생산 방법 및 그 포도당 |
| DK179660B1 (en) | 2016-04-08 | 2019-03-13 | Novozymes A/S | Stabilized Alpha-Amylase Variants and use of the same |
| BR112018072530A2 (pt) | 2016-05-03 | 2019-03-26 | Danisco Us Inc | variantes de protease e usos das mesmas |
| BR112018072586A2 (pt) | 2016-05-05 | 2019-02-19 | Danisco Us Inc | variantes de protease e usos das mesmas |
| EP3464599A1 (en) | 2016-05-31 | 2019-04-10 | Danisco US Inc. | Protease variants and uses thereof |
| DE102016209880A1 (de) | 2016-06-06 | 2017-12-07 | Henkel Ag & Co. Kgaa | Neue Amylasen |
| WO2017219011A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc | Protease variants and uses thereof |
| EP3275989A1 (en) | 2016-07-26 | 2018-01-31 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
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| EP3275986B1 (en) | 2016-07-26 | 2020-07-08 | The Procter and Gamble Company | Automatic dishwashing detergent composition |
| EP3275990A1 (en) * | 2016-07-28 | 2018-01-31 | The Procter and Gamble Company | Process for reblending a first liquid detergent composition into a second liquid detergent composition |
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| EP4353828A3 (en) | 2017-02-24 | 2024-07-24 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
| EP3583210B1 (en) | 2017-03-15 | 2021-07-07 | Danisco US Inc. | Trypsin-like serine proteases and uses thereof |
| EP3601553B1 (en) * | 2017-03-31 | 2025-12-03 | Danisco US Inc. | Alpha-amylase combinatorial variants |
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| CN111094576A (zh) | 2017-09-13 | 2020-05-01 | 丹尼斯科美国公司 | 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列 |
| DE102017125560A1 (de) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | Reinigungszusammensetzungen, die dispersine iii enthalten |
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2014
- 2014-03-11 DK DK14727646.3T patent/DK2970931T3/en active
- 2014-03-11 DK DK14722824.1T patent/DK2970930T4/da active
- 2014-03-11 US US14/775,603 patent/US20160017304A1/en not_active Abandoned
- 2014-03-11 EP EP14727646.3A patent/EP2970931B1/en active Active
- 2014-03-11 DK DK18151284.9T patent/DK3336183T4/da active
- 2014-03-11 CN CN201480026463.3A patent/CN105229148B9/zh active Active
- 2014-03-11 EP EP14722824.1A patent/EP2970930B2/en active Active
- 2014-03-11 CA CA2903027A patent/CA2903027A1/en active Pending
- 2014-03-11 BR BR112015021647-1A patent/BR112015021647B1/pt active IP Right Grant
- 2014-03-11 ES ES18151284T patent/ES2882517T5/es active Active
- 2014-03-11 US US14/775,595 patent/US20160017303A1/en not_active Abandoned
- 2014-03-11 EP EP18151284.9A patent/EP3336183B2/en active Active
- 2014-03-11 ES ES14722824T patent/ES2676895T5/es active Active
- 2014-03-11 US US14/775,610 patent/US20160017305A1/en not_active Abandoned
- 2014-03-11 WO PCT/US2014/023590 patent/WO2014164834A1/en not_active Ceased
- 2014-03-11 HU HUE14722824A patent/HUE039341T2/hu unknown
- 2014-03-11 WO PCT/US2014/023515 patent/WO2014164800A1/en not_active Ceased
- 2014-03-11 EP EP21166776.1A patent/EP3978604A1/en active Pending
- 2014-03-11 CN CN201910610194.XA patent/CN110777132B/zh active Active
- 2014-03-11 CN CN201480026423.9A patent/CN105229147B/zh active Active
- 2014-03-11 WO PCT/US2014/023458 patent/WO2014164777A1/en not_active Ceased
- 2014-03-11 EP EP14722410.9A patent/EP2970929A1/en not_active Withdrawn
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2019
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