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ES2537784B1 - Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program - Google Patents

Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program Download PDF

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Publication number
ES2537784B1
ES2537784B1 ES201300750A ES201300750A ES2537784B1 ES 2537784 B1 ES2537784 B1 ES 2537784B1 ES 201300750 A ES201300750 A ES 201300750A ES 201300750 A ES201300750 A ES 201300750A ES 2537784 B1 ES2537784 B1 ES 2537784B1
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ES
Spain
Prior art keywords
multiple wavelengths
holographic
line
sub
computer program
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES201300750A
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Spanish (es)
Other versions
ES2537784A1 (en
Inventor
Francisco Javier GARCÍA MONREAL
Vicente MICÓ SERRANO
Martín SANZ SABATER
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universitat de Valencia
Original Assignee
Universitat de Valencia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universitat de Valencia filed Critical Universitat de Valencia
Priority to ES201300750A priority Critical patent/ES2537784B1/en
Priority to PCT/ES2014/000135 priority patent/WO2015015023A1/en
Publication of ES2537784A1 publication Critical patent/ES2537784A1/en
Application granted granted Critical
Publication of ES2537784B1 publication Critical patent/ES2537784B1/en
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Classifications

    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/08—Synthesising holograms, i.e. holograms synthesized from objects or objects from holograms
    • G03H1/0866—Digital holographic imaging, i.e. synthesizing holobjects from holograms
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/04—Processes or apparatus for producing holograms
    • G03H1/0443—Digital holography, i.e. recording holograms with digital recording means
    • G03H2001/0447—In-line recording arrangement
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/22—Processes or apparatus for obtaining an optical image from holograms
    • G03H1/2249—Holobject properties
    • G03H2001/2263—Multicoloured holobject
    • G03H2001/2271—RGB holobject
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
    • G03H1/26—Processes or apparatus specially adapted to produce multiple sub- holograms or to obtain images from them, e.g. multicolour technique
    • G03H1/2645—Multiplexing processes, e.g. aperture, shift, or wavefront multiplexing
    • G03H2001/266—Wavelength multiplexing
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
    • G03H2222/00—Light sources or light beam properties
    • G03H2222/10—Spectral composition
    • G03H2222/17—White light
    • G03H2222/18—RGB trichrome light

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Computing Systems (AREA)
  • Theoretical Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Holo Graphy (AREA)
  • Image Processing (AREA)

Abstract

Método de reconstrucción holográfico basado en microscopia sin lentes en línea con múltiples longitudes de onda, microscopio holográfico sin lentes en línea basado en múltiples longitudes de onda y programa de ordenador#El método comprende:#- iluminar, de manera simultánea, un objeto (O) con luz divergente que incluye tres o más longitudes de onda ({la}{sub,1} + ({la}{sub,2} + ({la}{sub,3}+…);#- registrar un holograma múltiple del objeto iluminado (O); y#- obtener, por reconstrucción digital, una imagen holográfica compuesta mediante un procesado digital de la información multiespectral incluida en el holograma múltiple relativa a las tres o más longitudes de onda ({la}{sub,1} + ({la}{sub,2} + ({la}{sub,3}+…), que incluye realizar una mezcla ponderada en el dominio de Fourier de los espectros correspondientes a cada longitud de onda.#El microscopio está adaptado para implementar el método del primer aspecto.#El programa de ordenador implementa la reconstrucción digital del método de la invención.Holographic reconstruction method based on microscopy without in-line lenses with multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program # The method comprises: # - simultaneously illuminate an object (O ) with divergent light that includes three or more wavelengths ({la} {sub, 1} + ({la} {sub, 2} + ({la} {sub, 3} +…); # - register a multiple hologram of the illuminated object (O); and # - obtain, by digital reconstruction, a holographic image composed by digital processing of the multispectral information included in the multiple hologram relative to the three or more wavelengths ({la} {sub, 1 } + ({la} {sub, 2} + ({la} {sub, 3} +…), which includes performing a weighted mix in the Fourier domain of the spectra corresponding to each wavelength. # The microscope is adapted to implement the first aspect method. # The computer program implements the reconstruction of gital of the method of the invention.

Description

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Claims (1)

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ES201300750A 2013-08-02 2013-08-02 Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program Active ES2537784B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201300750A ES2537784B1 (en) 2013-08-02 2013-08-02 Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program
PCT/ES2014/000135 WO2015015023A1 (en) 2013-08-02 2014-07-25 Holographic reconstruction method using lensless in-line microscopy with multiple wavelengths, holographic lensless in-line microscope using multiple wavelengths and computer program

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201300750A ES2537784B1 (en) 2013-08-02 2013-08-02 Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program

Publications (2)

Publication Number Publication Date
ES2537784A1 ES2537784A1 (en) 2015-06-12
ES2537784B1 true ES2537784B1 (en) 2016-04-12

Family

ID=52431045

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201300750A Active ES2537784B1 (en) 2013-08-02 2013-08-02 Holographic reconstruction method based on in-line microscopy without multiple wavelengths, holographic microscope without in-line lenses based on multiple wavelengths and computer program

Country Status (2)

Country Link
ES (1) ES2537784B1 (en)
WO (1) WO2015015023A1 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3036800B1 (en) 2015-05-28 2020-02-28 Commissariat A L'energie Atomique Et Aux Energies Alternatives METHOD OF OBSERVING A SAMPLE
FR3049348B1 (en) * 2016-03-23 2023-08-11 Commissariat Energie Atomique METHOD FOR CHARACTERIZING A PARTICLE IN A SAMPLE
FR3056749B1 (en) 2016-09-28 2018-11-23 Commissariat A L'energie Atomique Et Aux Energies Alternatives METHOD FOR NUMBERING LEUKOCYTES IN A SAMPLE
US11373278B2 (en) 2016-09-30 2022-06-28 University Of Utah Research Foundation Lensless imaging device
EP3339963B1 (en) 2016-12-21 2020-08-12 IMEC vzw An apparatus and a method for in-line holographic imaging
CN111273533B (en) * 2019-09-27 2021-08-06 中国工程物理研究院激光聚变研究中心 A coaxial digital holographic automatic focusing method and system
CN110836867A (en) * 2019-10-18 2020-02-25 南京大学 Non-lens holographic microscopic particle characterization method based on convolutional neural network
WO2024123803A1 (en) * 2022-12-05 2024-06-13 Macula Vision Systems, Inc. Holographic optical system and method for automatic imaging and interpretation of biological samples
CN118295225B (en) * 2024-06-06 2024-09-13 南昌大学 A dual-channel prior-constrained multi-distance lensless digital holographic reconstruction method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3695744A (en) * 1971-01-14 1972-10-03 Rca Corp Holographic multicolor technique
US8842901B2 (en) * 2010-12-14 2014-09-23 The Regents Of The University Of California Compact automated semen analysis platform using lens-free on-chip microscopy

Also Published As

Publication number Publication date
ES2537784A1 (en) 2015-06-12
WO2015015023A1 (en) 2015-02-05

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