[go: up one dir, main page]

ES2523998T3 - Método de aislamiento de citoblastos dérmicos - Google Patents

Método de aislamiento de citoblastos dérmicos Download PDF

Info

Publication number
ES2523998T3
ES2523998T3 ES10817219.8T ES10817219T ES2523998T3 ES 2523998 T3 ES2523998 T3 ES 2523998T3 ES 10817219 T ES10817219 T ES 10817219T ES 2523998 T3 ES2523998 T3 ES 2523998T3
Authority
ES
Spain
Prior art keywords
cytoblast
dermal
isolation method
cells
positive cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10817219.8T
Other languages
English (en)
Inventor
Tsutomu Soma
Haruyo Yamanishi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shiseido Co Ltd
Original Assignee
Shiseido Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shiseido Co Ltd filed Critical Shiseido Co Ltd
Application granted granted Critical
Publication of ES2523998T3 publication Critical patent/ES2523998T3/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Rheumatology (AREA)
  • General Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Un método de aislamiento de citoblastos dérmicos que comprende: someter a células separadas de la piel por tratamiento enzimático a un cultivo en suspensión; y aislar las células positivas de marcadores de citoblastos de las células cultivadas, siendo las células positivas de marcadores de citoblastos las células positivas de CD34.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7

Claims (1)

  1. imagen1
ES10817219.8T 2009-09-15 2010-09-15 Método de aislamiento de citoblastos dérmicos Active ES2523998T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2009213291 2009-09-15
JP2009213291 2009-09-15
PCT/JP2010/065981 WO2011034106A1 (ja) 2009-09-15 2010-09-15 真皮幹細胞の単離方法

Publications (1)

Publication Number Publication Date
ES2523998T3 true ES2523998T3 (es) 2014-12-03

Family

ID=43758707

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10817219.8T Active ES2523998T3 (es) 2009-09-15 2010-09-15 Método de aislamiento de citoblastos dérmicos

Country Status (7)

Country Link
US (1) US8962323B2 (es)
EP (1) EP2479258B1 (es)
JP (1) JP5800712B2 (es)
KR (1) KR101717317B1 (es)
CN (1) CN102498205B (es)
ES (1) ES2523998T3 (es)
WO (1) WO2011034106A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103459599B (zh) * 2010-11-23 2017-06-16 库尔纳公司 通过抑制nanog的天然反义转录物而治疗nanog相关疾病
KR101751355B1 (ko) 2011-08-24 2017-07-04 (주)아모레퍼시픽 인간 피부 진피 유래 성체 줄기세포
US20150064141A1 (en) * 2012-04-05 2015-03-05 The Regents Of The University Of California Regenerative sera cells and mesenchymal stem cells
WO2015033558A1 (ja) * 2013-09-04 2015-03-12 株式会社大塚製薬工場 多能性幹細胞の調製方法
KR101700598B1 (ko) * 2014-11-11 2017-01-31 한국원자력의학원 Cd105를 포함하는 줄기세포능을 가진 섬유아세포 분리용 바이오마커 조성물 및 이를 이용한 줄기세포능을 가진 섬유아세포 분리 방법
EP3436035B1 (en) * 2016-03-29 2024-09-04 SmsBiotech, Inc. Compositions and methods for using small mobile stem cells
EP3656850A4 (en) 2017-07-18 2021-03-31 Shiseido Co., Ltd. MESENCHYMATIC STEM CELL INDUCTION AGENT
JP7258749B2 (ja) 2017-07-18 2023-04-17 株式会社 資生堂 抗老化物質のスクリーニング方法
CN114158274A (zh) * 2019-07-22 2022-03-08 株式会社资生堂 真皮鞘杯细胞的鉴别方法
CN110940568B (zh) * 2019-12-17 2022-02-18 武汉友芝友医疗科技股份有限公司 一种用于细胞富集、分离、染色、制片的一体化系统
CN113174365B (zh) * 2021-03-26 2024-10-25 万绵水 一种表皮干细胞的分离及培养方法和细胞悬液
TWI828062B (zh) * 2022-02-14 2024-01-01 陞醫生物科技股份有限公司 用於生產外泌體的方法及其所製得的外泌體暨其應用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070053884A1 (en) * 2003-05-16 2007-03-08 Kyowa Hakko Kogyo Co., Ltd Novel adult tissue-derived stem cell and use thereof
CN1253558C (zh) * 2003-09-02 2006-04-26 中国人民解放军第四军医大学口腔医学院 人表皮干细胞的分离与培养方法

Also Published As

Publication number Publication date
WO2011034106A1 (ja) 2011-03-24
EP2479258A1 (en) 2012-07-25
KR101717317B1 (ko) 2017-03-16
JP5800712B2 (ja) 2015-10-28
CN102498205B (zh) 2014-06-11
JPWO2011034106A1 (ja) 2013-02-14
EP2479258A4 (en) 2013-08-14
US8962323B2 (en) 2015-02-24
US20120208269A1 (en) 2012-08-16
EP2479258B1 (en) 2014-11-12
KR20120068856A (ko) 2012-06-27
CN102498205A (zh) 2012-06-13

Similar Documents

Publication Publication Date Title
ES2523998T3 (es) Método de aislamiento de citoblastos dérmicos
MX2016008251A (es) Medios para cultivo celular.
AU2019268194A1 (en) Cell culture system
BR112022017216A2 (pt) Método para produzir células exterminadoras naturais a partir de células-tronco pluripotentes
BR112013027307A2 (pt) método para tratar uma matriz de tecido; matriz de tecido acelular
EA201190218A1 (ru) Траустохитридиевые композиции жирных кислот и способы их получения и применения
PH12012501981A1 (en) Selective extraction of proteins from freshwater algae
RU2012105922A (ru) Дифференцирование человеческих эмбриональных стволовых клеток
AU2017254264A1 (en) Method for producing retinal tissue
AU2011372711B2 (en) Method for processing mesenchymal stem cells and the use thereof in the treatment of diseases associated with oxidative stress
MX2011008495A (es) Formulaciones de caldo de fermentacion.
BR112012005844A2 (pt) Método para cultivar microalgas capazes de crescimento heterotrófico, método para fabricar um material, e sistema de biorreator
EA201400151A1 (ru) Микроорганизмы, продуцирующие эйкозапентаеновую кислоту, композиции жирных кислот, способы их получения и применения
MX348269B (es) Metodo para producir lamina celular epitelial de pigmento retinal.
NZ609731A (en) Compositions of adult organ stem cells and uses thereof
MX2015007361A (es) Produccion mediada por la proteina portadora de acilo de los derivados de acidos grasos.
MY160740A (en) Method of producing lauric acid-containing oil or fat
CL2012000808A1 (es) Composicion que comprende un caldo o solido de fermentacion con actividad de un actinomiceto que comprenden una proporción optimizada entre las dunaimicinas activas y las dunaimicinas inactivas; proceso para producir un producto de fermentacion enriquecido en dunaimicinas con actividad insecticida; metodo para analizar presencia de una actividad insecticida mejorada en actinomicetos.
MX336787B (es) Microorganismos deseñados geneticamente que comprenden 4-hidroxibenzoil-coa tioesterasas y metodos de uso de los mismos para producir acidos grasos libes y derivados de acidos grasos.
EA201201278A1 (ru) Штаммы дрожжей и их использование при производстве липидов
MY186172A (en) Endogenous dnase activity to reduce dna content
WO2013184193A3 (en) Cancer stem cells and methods of using the same
BRPI1003783A2 (pt) Meio de cultura de células de mamíferos que compreende sobrenadante de estágios de fracionamento de cohn, seu processo de preparação e uso
BR112012014696A2 (pt) novas linhagens de levedura para produção de álcool
ATE488575T1 (de) Apokrine zelllinie