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ES2580172T3 - Una célula madre mesenquimal multipotente aislada de glomérulos humanos adultos (HGL-MSC), un método para preparar la misma y usos de la misma en la medicina regenerativa del riñón - Google Patents

Una célula madre mesenquimal multipotente aislada de glomérulos humanos adultos (HGL-MSC), un método para preparar la misma y usos de la misma en la medicina regenerativa del riñón Download PDF

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Publication number
ES2580172T3
ES2580172T3 ES09744160.4T ES09744160T ES2580172T3 ES 2580172 T3 ES2580172 T3 ES 2580172T3 ES 09744160 T ES09744160 T ES 09744160T ES 2580172 T3 ES2580172 T3 ES 2580172T3
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Prior art keywords
mesenchymal stem
hgl
msc
stem cell
adult human
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ES09744160.4T
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English (en)
Inventor
Giovanni Camussi
Stefania Bruno
Benedetta Bussolati
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Fresenius Medical Care Deutschland GmbH
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Fresenius Medical Care Deutschland GmbH
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0684—Cells of the urinary tract or kidneys
    • C12N5/0687—Renal stem cells; Renal progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/12—Drugs for disorders of the urinary system of the kidneys
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Rheumatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Una célula madre mesenquimal glomerular multipotente aislada de glomérulos de riñón humano adulto (hGLMSC), en donde dicha célula está caracterizada por los marcadores CD133 (negativas), CD146 (positivas), CD34 (negativas), CD105 (positivas), CD24 (positivas) y Pax-2 (positivas) y caracterizada adicionalmente por la capacidad de diferenciarse en al menos los siguientes tipos de células: podocitos, células endoteliales y células mesangiales.

Description

imagen1
imagen2
imagen3
imagen4
imagen5
imagen6
imagen7
glomerulares humanas (hGL-MSC) de la invención.
Tabla 1
Marcador
hGL-MSC
CD133
Negativo
CD146
100%
CD90
100%
CD44
100%
CD29
100%
CD73
100%
CD105
100%
CD106
-
CD166
100%
CD45
Negativo
CD34
Negativo
CD31
Negativo
CD24
100%
SCA-1
-
Alfa-SMA
Negativo
Vimentina
100%
Citoqueratina
Negativo
Nestina
100%
Nanog
100%
Musashi
100%
Oct-4
Negativo
Pax-2
100%
MHC-1
100%
MHC-2
Negativo
CD80
Negativo
CD86
Negativo
CD40
Negativo
9
imagen8
imagen9
imagen10
imagen11
38. Lazzeri E, Crescioli C, Ronconi E, Mazzinghi B, Sagrinati C, Netti GS,Angelotti ML, Parente E, Ballerini L, Cosmi L, Maggi L, Gesualdo L, Rotondi M, Annunziato F, Maggi E, Lasagni L, Serio M, Romagnani S, Vannelli GB, Romagnani P. Regenerative potential of embryonic renal multipotent progenitors in acute renal failure. J Am Soc Nephrol. 2007;18: 3128-3138.
5 39. Dekel B, Zangi L, Shezen E, Reich-Zeliger S, Eventov-Friedman S, Katchman H, Jacob-Hirsch J, Amariglio N, Rechavi G, Margalit R, Reisner Y. Isolation and characterization of nontubular sca-1+linmultipotent stem/progenitor cells from adult mouse kidney. J Am Soc Nephrol. 2006; 17: 3300-3314.
40. Hoogduijn MJ, Crop MJ, Peeters AM, Van Osch GJ, Balk AH, Ijzermans JN, Weimar W, Baan CC. Human heart,
spleen, and perirenal fat-derived mesenchymal stem cells have immunomodulatory capacities. Stem Cells Dev. 10 2007; 16: 597-604.
14

Claims (1)

  1. imagen1
    imagen2
ES09744160.4T 2008-11-04 2009-11-02 Una célula madre mesenquimal multipotente aislada de glomérulos humanos adultos (HGL-MSC), un método para preparar la misma y usos de la misma en la medicina regenerativa del riñón Active ES2580172T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08425708A EP2186883A1 (en) 2008-11-04 2008-11-04 An isolated multipotent mesenchymal stem cell from human adult glomeruli (hGL-MSC), a method of preparing thereof and uses thereof in the regenerative medicine of the kidney
EP08425708 2008-11-04
PCT/EP2009/064468 WO2010052192A1 (en) 2008-11-04 2009-11-02 An isolated multipotent mesenchymal stem cell from human adult glomeruli (hgl-msc), a method of preparing thereof and uses thereof in the regenerative medicine of the kidney

Publications (1)

Publication Number Publication Date
ES2580172T3 true ES2580172T3 (es) 2016-08-19

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ES09744160.4T Active ES2580172T3 (es) 2008-11-04 2009-11-02 Una célula madre mesenquimal multipotente aislada de glomérulos humanos adultos (HGL-MSC), un método para preparar la misma y usos de la misma en la medicina regenerativa del riñón

Country Status (7)

Country Link
US (1) US9499796B2 (es)
EP (2) EP2186883A1 (es)
JP (1) JP5795737B2 (es)
CN (1) CN102292434B (es)
ES (1) ES2580172T3 (es)
PL (1) PL2361303T3 (es)
WO (1) WO2010052192A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2363136A1 (en) 2010-03-02 2011-09-07 Fresenius Medical Care Deutschland GmbH Microvesicles (MVs) derived from adult stem cells for use in the therapeutic treatment of a tumor disease
NZ603482A (en) 2010-05-12 2014-11-28 Tengion Inc Bioactive renal cells
WO2012030968A1 (en) * 2010-08-31 2012-03-08 Renovocyte, LLC Systemic, allogenic stem cell therapies for treatment of diseases in animals
WO2012088225A2 (en) * 2010-12-22 2012-06-28 Tulane University Method for identifcation and culture of multipotent mesenchymal stem cells with high proliferation potential
ES2675693T3 (es) 2011-07-06 2018-07-11 Cell Therapy Limited Células progenitoras de linaje mesodérmico
BR112014007676A2 (pt) * 2011-09-30 2017-04-18 The Univ Of Miami células-tronco renais isoladas do rim
WO2014066699A1 (en) 2012-10-24 2014-05-01 Tengion, Inc. Renal cell populations and uses thereof
WO2014113704A2 (en) 2013-01-18 2014-07-24 Escape Therapeutics, Inc. Enhanced differentiation of mesenchymal stem cells
CN104977414B (zh) * 2014-04-09 2016-09-28 上海交通大学医学院 鉴定骨髓中是否存在保护白血病干细胞微环境的试剂盒及其应用
KR102415441B1 (ko) * 2014-08-21 2022-07-01 아지노모토 가부시키가이샤 간엽계 줄기 세포용 배지
US20180059109A1 (en) * 2016-08-31 2018-03-01 Meridigen Biotech Co., Ltd. Method of distinguishing mesenchymal stem cells and method of determining purity of mesenchymal stem cells
CN106265745A (zh) * 2016-09-30 2017-01-04 广州赛莱拉干细胞科技股份有限公司 一种干细胞制剂及其在制备治疗慢性肾炎的药物中的应用
JP7072777B2 (ja) * 2018-07-03 2022-05-23 株式会社生命科学インスティテュート 慢性腎障害治療のための多能性幹細胞
CA3182658A1 (en) 2020-05-08 2021-11-11 Gallant Pet, Inc. Uterine-derived regenerative cell compositions and uses thereof
CN117871216B (zh) * 2024-03-12 2024-06-04 中日友好医院(中日友好临床医学研究所) 一种石蜡标本中肾小球裂隙膜的三维可视化方法

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* Cited by examiner, † Cited by third party
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JP4085410B2 (ja) * 2003-03-14 2008-05-14 株式会社 バイオリンクインク 腎臓幹細胞／前駆細胞、腎臓幹細胞／前駆細胞の分離方法、及び腎疾患の治療方法
ITFI20060099A1 (it) * 2006-04-28 2007-10-29 Azienda Ospedaliera Careggi Popolazione di cellule staminali renali, sua identificazione ed uso per finalita' terapeutiche
CN101595212B (zh) * 2006-10-12 2014-04-30 伊西康公司 肾源细胞及在组织修复和再生中的使用方法

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JP5795737B2 (ja) 2015-10-14
US20110256111A1 (en) 2011-10-20
EP2361303A1 (en) 2011-08-31
JP2012507304A (ja) 2012-03-29
EP2186883A1 (en) 2010-05-19
CN102292434B (zh) 2014-03-12
CN102292434A (zh) 2011-12-21
US9499796B2 (en) 2016-11-22
WO2010052192A1 (en) 2010-05-14
EP2361303B8 (en) 2016-08-10
EP2361303B1 (en) 2016-04-06
PL2361303T3 (pl) 2016-10-31

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