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ES2365447T8 - Cuantificación del arnm de células individuales con rt-pcr en tiempo real. - Google Patents

Cuantificación del arnm de células individuales con rt-pcr en tiempo real. Download PDF

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Publication number
ES2365447T8
ES2365447T8 ES08749213T ES08749213T ES2365447T8 ES 2365447 T8 ES2365447 T8 ES 2365447T8 ES 08749213 T ES08749213 T ES 08749213T ES 08749213 T ES08749213 T ES 08749213T ES 2365447 T8 ES2365447 T8 ES 2365447T8
Authority
ES
Spain
Prior art keywords
pcr
sample
quantification
real
time
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES08749213T
Other languages
English (en)
Other versions
ES2365447T3 (es
Inventor
Martin Bengtsson
Michael Kubista
Anders Stahlberg
Linda Stroembom
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
TATAA BIOCENTER AB
TATAA BIOCT AB
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Original Assignee
TATAA BIOCENTER AB
TATAA BIOCT AB
F Hoffmann La Roche AG
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by TATAA BIOCENTER AB, TATAA BIOCT AB, F Hoffmann La Roche AG, Roche Diagnostics GmbH filed Critical TATAA BIOCENTER AB
Application granted granted Critical
Publication of ES2365447T3 publication Critical patent/ES2365447T3/es
Publication of ES2365447T8 publication Critical patent/ES2365447T8/es
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
ES08749213T 2007-05-03 2008-04-29 Cuantificación del arnm de células individuales con rt-pcr en tiempo real. Active ES2365447T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008961 2007-05-03
EP07008961 2007-05-03

Publications (2)

Publication Number Publication Date
ES2365447T3 ES2365447T3 (es) 2011-10-05
ES2365447T8 true ES2365447T8 (es) 2011-11-21

Family

ID=38050036

Family Applications (2)

Application Number Title Priority Date Filing Date
ES08749214.6T Active ES2472765T3 (es) 2007-05-03 2008-04-29 Lisis y transcripción inversa mejoradas para la cuantificación de ARNm
ES08749213T Active ES2365447T3 (es) 2007-05-03 2008-04-29 Cuantificación del arnm de células individuales con rt-pcr en tiempo real.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES08749214.6T Active ES2472765T3 (es) 2007-05-03 2008-04-29 Lisis y transcripción inversa mejoradas para la cuantificación de ARNm

Country Status (5)

Country Link
US (3) US8642266B2 (es)
EP (2) EP2147120B1 (es)
AT (1) ATE510932T1 (es)
ES (2) ES2472765T3 (es)
WO (2) WO2008135197A1 (es)

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* Cited by examiner, † Cited by third party
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EP2147120B1 (en) * 2007-05-03 2014-04-02 Roche Diagnostics GmbH Improved lysis and reverse transcription for mrna quantification
ES2539856T3 (es) 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
US9404924B2 (en) * 2008-12-04 2016-08-02 Massachusetts Institute Of Technology Method of performing one-step, single cell RT-PCR
KR101550086B1 (ko) 2008-12-04 2015-09-03 메사추세츠 인스티튜트 오브 테크놀로지 알레르기 반응의 진단 방법
US20120058520A1 (en) * 2009-05-14 2012-03-08 Wako Pure Chemical Industries, Ltd. Method for synthesis of double-stranded dna corresponding to rna, and method for amplification of the dna
US9486803B2 (en) * 2010-01-22 2016-11-08 Biotix, Inc. Pipette tips
WO2011104027A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Process for parallel isolation and/or purification of rna and dna
KR101420094B1 (ko) 2010-10-27 2014-07-17 (주)바이오니아 다양한 생체시료분석을 위한 전자동실시간정량증폭장비, 다양한 생체시료분석을 위한 자동정제 및 반응준비 장치, 전자동 핵산정제 및 실시간 정량 유전자증폭 방법, 전자동 핵산정제방법, 실시간정량ｐｃｒ을 이용한 병원균의 전자동 생균수검사방법, 정량면역ｐｃｒ을 이용한 전자동 항원농도획득방법 및 타겟항원에 라벨링된 부착용 타겟핵산의 정제방법
US8795606B2 (en) 2012-05-30 2014-08-05 Biotix, Inc. Integrated pipette tip devices
US20150246335A1 (en) * 2012-09-13 2015-09-03 President And Fellows Of Harvard College Methods for multiplex analytical measurements in single cells of solid tissues
AU2013333763A1 (en) * 2012-10-20 2015-05-14 Selfdiagnostics Ou Method and its compositions for detection of nucleic acid target from biological samples and body fluids
DE102015017080B3 (de) 2014-02-28 2024-01-04 Gen-Probe Incorporated Verfahren zur Isolierung von Nukleinsäure von zytologischen Proben in flüssigen Konservierungsmitteln, die Formaldehyd enthalten
JP6742311B2 (ja) 2014-11-14 2020-08-19 アテナ ダイアグナスティクス，インコーポレイテッド サイレントキャリア遺伝子型を検出する方法
JP2017209036A (ja) * 2016-05-24 2017-11-30 東洋紡株式会社 改良されたウイルス検出方法
CN114453042A (zh) 2017-05-17 2022-05-10 拜欧迪克斯公司 人体工学移液管吸头
GB201803654D0 (en) 2018-03-07 2018-04-25 Arcticzymes As Thermolabile proteinases
CN113557298B (zh) * 2019-03-13 2024-08-27 东洋纺株式会社 核酸的生成和扩增
JPWO2021221109A1 (es) * 2020-04-30 2021-11-04

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* Cited by examiner, † Cited by third party
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GB9315448D0 (en) * 1993-07-26 1993-09-08 Rank Xerox Ltd Recording and retrieval of information relevant to the activities of a user
US5637687A (en) * 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US7034864B2 (en) * 2000-05-19 2006-04-25 Canon Kabushiki Kaisha Image display apparatus, image display system, and image display method
US7277414B2 (en) * 2001-08-03 2007-10-02 Honeywell International Inc. Energy aware network management
US7004401B2 (en) * 2001-08-10 2006-02-28 Cerys Systems, Inc. System and method for regulating agriculture storage facilities in order to promote uniformity among separate storage facilities
EP2163652B2 (en) * 2002-01-28 2023-05-31 Life Technologies Corporation Preparing crude biological extracts using protease, suitable for preparing cDNA
US20040139110A1 (en) * 2002-12-31 2004-07-15 Lamarca Anthony G. Sensor network control and calibration system
US20040144849A1 (en) * 2003-01-28 2004-07-29 Osman Ahmed Building control system using integrated MEMS devices
DE602004030785D1 (de) * 2003-05-19 2011-02-10 Univ Brandeis Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren
US9626862B2 (en) * 2003-07-07 2017-04-18 Industrial Technology Research Institute Methods and systems for operating a logical sensor network
JP3714940B2 (ja) * 2003-11-07 2005-11-09 株式会社日立ハイテクノロジーズ Ｒｎａ抽出方法、および生体材料の分析方法
US20050208501A1 (en) * 2004-03-16 2005-09-22 Ambion, Inc. Process and reagents for extraction of RNA from fractionated blood leukocytes
DE102004026744A1 (de) * 2004-05-28 2005-12-29 Philipps-Universität Marburg Erfindung betreffend cDNA-Herstellung aus Zellen nach Laser-Mikrodissektion
US20070214133A1 (en) * 2004-06-23 2007-09-13 Edo Liberty Methods for filtering data and filling in missing data using nonlinear inference
US20060286557A1 (en) * 2005-06-15 2006-12-21 Basehore Lee S Combined lysis and PCR buffer
US20080003574A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Kits for RNA extraction
EP2147120B1 (en) * 2007-05-03 2014-04-02 Roche Diagnostics GmbH Improved lysis and reverse transcription for mrna quantification
US9848058B2 (en) * 2007-08-31 2017-12-19 Cardiac Pacemakers, Inc. Medical data transport over wireless life critical network employing dynamic communication link mapping
US20090195401A1 (en) * 2008-01-31 2009-08-06 Andrew Maroney Apparatus and method for surveillance system using sensor arrays
ES2539856T3 (es) * 2008-08-01 2015-07-06 F. Hoffmann-La Roche Ag Lisis mejorada y transcripción reversa para la cuantificación de mRNA
JP5134527B2 (ja) * 2008-12-25 2013-01-30 川崎重工業株式会社 自動二輪車のバンク角検知装置およびヘッドランプ装置

Also Published As

Publication number Publication date
US9200313B2 (en) 2015-12-01
US20100216194A1 (en) 2010-08-26
ES2365447T3 (es) 2011-10-05
ES2472765T3 (es) 2014-07-03
EP2147119B1 (en) 2011-05-25
EP2147119A1 (en) 2010-01-27
WO2008135197A1 (en) 2008-11-13
US8642266B2 (en) 2014-02-04
EP2147120A1 (en) 2010-01-27
ATE510932T1 (de) 2011-06-15
WO2008135196A1 (en) 2008-11-13
US20160168631A1 (en) 2016-06-16
EP2147120B1 (en) 2014-04-02
US20110136180A1 (en) 2011-06-09

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