ES2360435A1 - Derivatives of biss (aralquil) amino and systems (6 5) -heteroaromaticos and its use in the treatment of neurodegenerative pathologies, including alzheimer's disease (Machine-translation by Google Translate, not legally binding) - Google Patents

Abstract

The present invention, which is included in the field of pharmaceutical research and industry, refers to chemical compounds derived from bis(aralkyl)amino and [6+5]heteroaryl, to the processes for their preparation, to the pharmaceutical compositions that they contain and their use for the manufacture of a medicine for the treatment of cognitive disorders. In particular, it deals with compounds and compositions that protect neurons from mitochondrial oxidative stress, that capture radical oxygen species (ROS), that increase the levels of the neurotransmitter acetylcholine and arrest neurodegeneration related to {be}-amyloid peptide dysfunction. These pharmacological properties are useful for the treatment of neurodegenerative pathologies, including Alzheimer's disease.

Description

Derivatives of bis (aralkyl) amino and [6 + 5] -heteroaromatic systems and their use in the treatment of neurodegenerative pathologies, including disease Alzheimer's

The present invention, which is included in the field of research and pharmaceutical industry, refers to chemical compounds derived from bis (aralkyl) amino and [6 + 5] heteroaryl, to the procedures for its preparation, to the pharmaceutical compositions that contain them and their use for manufacture of a medication for the treatment of disorders Cognitive In particular, it deals with compounds and compositions that protect the neurons from mitochondrial oxidative stress, which capture oxygen radical species (ROS), which increase the levels of the neurotransmitter acetylcholine and that stop the neurodegeneration related to peptide dysfunction β-amyloid. These pharmacological properties they are useful for the treatment of neurodegenerative pathologies, including Alzheimer's disease.

Prior art

Alzheimer's disease (AD), the most dementia common in elderly people, it is a pathology complex neurodegenerative central nervous system, characterized by a progressive loss of capabilities intellectuals (memory, language and reasoning) and disorders psychiatric (apathy, anxiety, depression, aggressiveness). But not its etiology is fully known, there are several characteristics of the disease that play an important role in this pathology, as senile plates (deposits of β-amyloid abnormal metabolism derivatives of the amyloid precursor protein), the neurofibrillary tangles (composed of abnormally hyperphosphorylated tau protein), the oxidative damage in various cellular structures and low levels of the neurotransmitter acetylcholine.

Current treatments are fundamentally symptomatic. In recent decades, the cholinergic approach has put four drugs on the market for the treatment of the disease: the inhibitors of the enzyme acetylcholinesterase (AChE) tacrine, donepezil, rivastigmine and galantamine, which increase neurotransmission in the cholinergic synapses of the brain, palliating cognitive deficits (Villarroya, M. et al., Expert Opin. Investig. Drugs 2007, 16 , 1987-1998). So far, the only approved non-cholinergic drug is memantine, an N- methyl-D-aspartate receptor antagonist, which increases memory and intellectual abilities by modulating the glutamatergic system (Parsons, CG et al. , Neuropharmacology 2007, 53 , 699-723). The drugs approved for the treatment of Alzheimer's disease are shown below:

one

The AChE enzyme has two important sites: the catalytic active center (CAS) where hydrolysis of acetylcholine and found at the bottom of a throat narrow, and peripheral anionic site (PAS) located in the entrance of the catalytic throat.

In addition to its role in cholinergic transmission, AChE has other functions related to neuronal differentiation, cell adhesion and amyloid peptide aggregation. Different biochemical studies have shown that AChE favors the formation of β-amyloid aggregates (Aβ), establishing AChE-Aβ complexes that are more toxic than the isolated Aβ itself. Since the point of adhesion between the enzyme and the peptide is located in the PAS, dual AChE inhibitors, capable of interacting simultaneously with the CAS and PAS sites, are of great interest in AD since they can alleviate cognitive deficits and stop Aβ-related neurotoxicity (from Ferrari, GV et al., Biochemistry 2001, 40 , 10447-10457). In recent years, different families of dual AChE inhibitors have been described (for example, Fernández-Bachiller, MI et al., ChemMedChem 2009, 4 , 828-841; Muñoz-Torrero, D., Curr. Med. Chem . 2008, 15, 2433-2455).

On the other hand, the endogenous antioxidant system decreases with age and there is clear evidence of the implication of oxidative stress in the onset and progression of neurodegenerative diseases, including AD (Nunomura, A. et al., J. Neuropathol. Exp. Neurol 2006, 65 , 631-641). Specifically, in EA recent studies have shown that oxidative damage is a fact that precedes the appearance of other lesions characteristic of the disease, such as senile plaques and neurofibrillar clews (Gu, F. et al., Neurosci. Lett . 2008, 440 , 44-48; Moreira, PI et al., CNS Neurol. Disord. Drug Targets 2008, 7 , 3-10; Goldsbury, C. et al., Aging Cell 2008, 7 , 771-775). Therefore, products capable of protecting neurons against oxidative stress are beneficial, both for the prevention and for the treatment of neurodegenerative diseases, among which is Alzheimer's disease EA (Zhang, HY et al., Drug Discov. Today 2006, 11 , 749-754).

Melatonin is an endogenous substance, with a [6 + 5] -heteroaromatic structure, which is secreted by the pineal gland and whose levels decrease dramatically in patients affected by AD. Melatonin has a potent antioxidant action, capturing a wide variety of reactive oxygen species (ROS). It also stimulates the biosynthesis of other endogenous antioxidant enzymes, improves mitochondrial metabolism, reduces tau hyperphosphorylation and has neuroprotective actions against Aβ (Pandi-Perumal, SR et al., FEBS J. 2006, 273 , 2813-2838; Reiter, RJ et al., Adv. Med. Sci . 2007, 52 , 11-28; Tomás-Zapico, C. et al., J. Pineal Res . 2005, 39 , 99-104).

Brief Description of the Invention

In a first aspect, the present invention is refers to a compound of formula (I)

2

where

R1 to R15 are independently selected from hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted) , aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), COR_a, C (O) OR_a, C (O) NR_R_ {b}, C = NR_ { a}, CN, OR_ {a}, OC (O) R_ {a},
S (O) r -R_ {a}, NR_ {a} R_ {b}, NR_ {a} C (O) R_ {b}, NO_ {2}, N = CR_ {a} R_ {b } or halogen;

R_ {a} and R_ {b} are selected independently between hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted), aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), or halogen, with the proviso that they are not halogens when they are attached to an N.

r is selected from 0, 1 or 2.

j and k are numbers that are selected independently between 1 and 8.

A, B, D and E are independently selected between CR_ {a} R_ {b}, CR_ {a} = CR_ {b}, CO, O, S, or NR_ {a}; where R_ {a} and R_ {b} are defined as above;

m, n, p, and q are numbers that are selected regardless of a value between 0 and 10, with the proviso that its sum is at least four, m + n + p + q \ geq 4

X and Y are independently selected from CH or N; Z is selected from CH, O, S or N, with the proviso that at minus one of X, Y or Z is a heteroatom;

when Z is C or N, then R 11 is select from hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or non-substituted alkenyl substituted, substituted or unsubstituted aryl, substituted heterocycle or unsubstituted, substituted or unsubstituted alkoxy or aryloxy substituted or unsubstituted; when Z is O or S, then R_ {11} does not exists.

the spacer [A] m - [B] n - [D] p - [E] q It is suitable as a substituent in X or Y when X or Y are C, the which are C instead of CH.

or an isomer, a pharmaceutically salt acceptable, a pro-drug or a solvate of same.

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The present invention also relates to the use of the above defined compounds of general formula I in the manufacture of a medicament or pharmaceutical composition with neuroprotective, antioxidant and cholinergic properties, preferably for the treatment of cognitive disorders such as senile dementia, vascular dementia, cognitive impairment, attention deficit disorder and / or disease neurodegeneratives such as Alzheimer's disease, pathologies prionics (e.g. Creutzfeld-Jacob disease), the Parkinson's disease, systemic amyloidosis, lateral sclerosis Amyotrophic and related ailments.

Detailed description of the invention

The authors of the present invention have designed new multifunctional products derived from bis (aralkyl) amino- [6 + 5] heteroaryl that combine neuroprotective, antioxidant and cholinergic properties in a low molecular weight molecule. They are potent neuroprotectors against mitochondrial oxidative stress, they are able to capture reactive oxygen species (ROS) and increase the levels of acetylcholine neurotransmitter by binding with the CAS of the ACHE In addition, these products join the AChE PAS by inhibiting Aβ aggregation mediated by AChE. In addition, they are not toxic and would cross the blood brain barrier to reach to its therapeutic targets located in the CNS.

The compounds of the invention are characterized by two aromatic fragments derived respectively from bis (aralkyl) amine and a heterocycle [6 + 5], attached by a suitable connector. Its biological properties can be modulated by varying the nature of the aromatic fragments and the spacer, as well as modifying the number and nature of substituents of the aromatic rings and the spacer, as well as the length of the latter. As will be shown with the examples, the Compounds of the invention are not toxic, present interesting neuroprotective properties against oxidative stress mitochondrial, are able to capture ROS free radicals, inhibit AChE interacting simultaneously with the CAS and PAS sites of the enzyme would inhibit the aggregation of Aβ and penetrate the SNC

In a first aspect, the present invention is refers to a compound of formula (I)

3

where

R1 to R15 are independently selected from hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted) , aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), COR_a, C (O) OR_a, C (O) NR_R_ {b}, C = NR_ { a}, CN, OR_ {a}, OC (O) R_ {a},
S (O) r -R_ {a}, NR_ {a} R_ {b}, NR_ {a} C (O) R_ {b}, NO_ {2}, N = CR_ {a} R_ {b } or halogen;

R_ {a} and R_ {b} are selected independently between hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted), aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), or halogen, with the proviso that they are not halogens when they are attached to an N.

r is selected from 0, 1 or 2.

j and k are numbers that are selected independently between 1 and 8.

A, B, D and E are independently selected between CR_ {a} R_ {b}, CR_ {a} = CR_ {b}, CO, O, S, or NR_ {a}; where R_ {a} and R_ {b} are defined as above;

m, n, p, and q are numbers that are selected regardless of a value between 0 and 10, with the proviso that its sum is at least four, m + n + p + q \ geq 4.

X and Y are independently selected from CH or N; Z is selected from CH, O, S or N, with the proviso that at minus one of X, Y or Z is a heteroatom;

when Z is C or N, then R 11 is select from hydrogen, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or non-substituted alkenyl substituted, substituted or unsubstituted aryl, substituted heterocycle or unsubstituted, substituted or unsubstituted alkoxy or aryloxy substituted or unsubstituted; when Z is O or S, then R_ {11} does not exists.

the spacer [A] m - [B] n - [D] p - [E] q It is suitable as a substituent in X or Y when X or Y are C, the which are C instead of CH.

or an isomer, a pharmaceutically salt acceptable, a pro-drug or a solvate of same.

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The term "alkyl" refers, in the present invention, to radicals of hydrocarbon chains, linear or branched, having 1 to 10 carbon atoms, preferably 1 to 4, and which are attached to the rest of the molecule by a single bond, for example, methyl, ethyl, n -propyl, i -propyl, n -butyl, tert-butyl , sec -butyl, n-pentyl, n-hexyl, etc. The alkyl groups may be optionally substituted by one or more substituents such as halogen, hydroxyl, alkoxy, carboxyl, carbonyl, cyano, acyl, alkoxycarbonyl, amino, nitro, mercapto and alkylthio.

The term "alkenyl" refers to hydrocarbon chain radicals containing one or more bonds double carbon-carbon, for example, vinyl, 1-propenyl, allyl, isoprenyl, 2-butenyl, 1,3-butadienyl, etc. Alkenyl radicals may be optionally substituted by one or more substituents such as halo, hydroxyl, alkoxy, carboxyl, cyano, carbonyl, acyl, alkoxycarbonyl, amino, nitro, mercapto and alkylthio.

"Cycloalkyl" refers herein invention, to a stable monocyclic or bicyclic radical of 3 to 10 members, which is saturated or partially saturated, and that only consists of carbon and hydrogen atoms, such as cyclopentyl, cyclohexyl or adamantyl and which may be optionally substituted by one or more groups such as alkyl, halogen, hydroxyl, alkoxy, carboxyl, cyano, carbonyl, acyl, alkoxycarbonyl, amino, nitro, mercapto and alkylthio.

The term "aryl" refers to the present invention to a phenyl, naphthyl, indenyl radical, phenanthryl or anthracil. The aryl radical may optionally be substituted by one or more substituents such as alkyl, haloalkyl, aminoalkyl, dialkylamino, hydroxyl, alkoxy, phenyl, mercapto, halogen, nitro, cyano and alkoxycarbonyl.

The term "heterocycle" refers, in the present invention, to a stable monocyclic or bicyclic radical of 3 to 15 members, which is unsaturated, saturated or partially saturated, and consisting of carbon atoms and at least one heteroatom selected from the group consisting of nitrogen, oxygen or sulfur Preferably it has 4 to 8 members with one or more heteroatoms and more preferably 5 to 6 members with one or more heteroatoms For the purpose of this invention the heterocycle it can be a monocyclic, bicyclic or tricyclic system, which can include fused rings. The atoms of nitrogen, carbon and sulfur of the heterocyclic radical may optionally be rusty; nitrogen atoms may optionally be quaternized and the heterocyclic radical may be partial or Fully saturated or be aromatic. Examples of heterocycles can be, not limitatively: azepines, idols, imidazoles, isothiazoles, thiadiazoles, furan, tetrahydrofuran, benzimidazole, benzothiazole, piperidine, piperazine, purine, quinoline.

The term "aryloxy" refers to a radical of formula Ar-O-R, where Ar it is an aryl group and R is an alkyl group as defined previously.

The term "alkoxy" refers to a radical of formula O-R where R is an alkyl group such as defined above.

Halogen refers to fluorine, chlorine, bromine or iodine.

In a preferred embodiment, R 3 -R 5 and R 7 -R 10 they are H.

In a preferred embodiment, R 6 is alkyl C_ {1} -C_ {4}. In a more preferred embodiment, R 6 is CH 3.

In a preferred embodiment, j and k are 1.

In a preferred embodiment, R1 and R2 are independently selected from hydrogen, halogen or alkoxy

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In a preferred embodiment, the present invention refers to a compound of formula (II)

4

where R 1, R 2, R 6, R 11 -R 15 A, B, D, E, m, n, p, q, X, Y, Z, are defined as previously.

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Preferably, one of X and Y is C and the other CH.

Preferably Z is N.

Preferably, R 11, R 12 and R 15 they are H.

In another preferred embodiment, the present invention refers to a compound of formula (III)

5

where R 1, R 2, R 6, R 13, R 14, A, B, D, E, m, n, p, q are defined as in the claim one.

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Preferably, R 13 and R 14 are independently select from H, halogen, OH, or alkoxy

Preferably, m + n + p + q is a value that is select from 4, 5, 6, 7, 8, 9, or 10. More preferably m + n + p + q It is 4.

In a preferred embodiment, the spacer [A] m - [B] n - [D] p - [E] q is selected from the formulas (CH 2) _ {r} CO-NR_ {a} -
(CH 2) s -, - (CH 2) r -NR a -CO- (CH 2) s -, (CH 2) _ {r} -CO-O- (CH2) s -, - (CH2) r O-CO- (CH2) s -, where R_ { a} is defined as in claim 1; rys are independently selected from 0 to 8.

Preferably spacer has the formula - (CH 2) r -CO-NR a - (CH 2) s -. More preferably, r is 0 and s is 2. More preferably, R_a it's H.

In another preferred embodiment, the present invention refers to a compound that is selected from the following group:

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - ((benzyl (methyl) amino) methyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-hydroxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-fluoro-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-chlorobenzyl) (methyl) amino) methyl) benzamide;

?

4 - (((2-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-chlorobenzyl) (methyl) amino) methyl) benzamide;

?

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((3-methoxybenzyl) methyl) amino) methyl) benzamide;

or an isomer, a pharmaceutically salt acceptable, a pro-drug or a solvate of same.

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The compounds of the present invention represented by formula (I) may include isomers, depending on the presence of multiple links (for example, Z, E), including optical isomers or enantiomers, depending on the presence of chiral centers. Isomers, enantiomers or individual diastereoisomers and mixtures thereof fall within the scope of the present invention, that is, the term Isomer also refers to any mixture of isomers, such as diastereomers, racemic, etc., even their optically isomers assets or mixtures in different proportions thereof. The single enantiomers or diastereoisomers, as well as mixtures thereof, They can be separated by conventional techniques.

Also, within the scope of this invention the prodrugs of the compounds of formula (I) are found. He term "prodrug" or "prodrug" as used herein includes any compound derived from a compound of formula (I) -for example and not limitation: esters (including esters of carboxylic acids, amino acid esters, phosphate esters, sulphonate esters of metal salts, etc.), carbamates, amides, etc.- that when administered to an individual can be transformed directly or indirectly in said compound of formula (I) in the mentioned individual. Advantageously, said derivative is a compound which increases the bioavailability of the compound of formula (I) when is given to an individual or that enhances the release of compound of formula (I) in a biological compartment. The nature of that derivative is not critical as long as you can be administered to an individual and provide the compound of formula (I) in a biological compartment of an individual. The preparation of said prodrug can be carried out by Conventional methods known to those skilled in the art.

The compounds of the invention may be in crystalline form as free compounds or as solvates. In this meaning, the term "solvate", as used here, includes both pharmaceutically acceptable solvates, that is, solvates of the compound of formula (I) that can be used in the preparation of a medicine, such as pharmaceutically solvates acceptable, which may be useful in the preparation of solvates or pharmaceutically acceptable salts. The nature of Pharmaceutically acceptable solvate is not critical as long as Be pharmaceutically acceptable. In a particular embodiment, the Solvate is a hydrate. Solvates can be obtained by methods conventional solvation known by experts in the matter.

For its application in therapy, the compounds of formula (I), its salts, prodrugs or solvates, will be found, preferably, in a pharmaceutically acceptable form or substantially pure, that is, it has a level of purity pharmaceutically acceptable excluding pharmaceutical additives normal such as diluents and carriers, and not including Material considered toxic at normal dosage levels. The purity levels for the active ingredient are preferably greater than 50%, more preferably greater than 70%, and still more preferably greater than 90%. In a preferred embodiment, are greater than 95% of the compound of formula (I), or of its salts, solvates or prodrugs.

The compounds of the present invention of formula (I) can be obtained or produced by a route synthetic chemistry or obtained from a natural matter of different origin.

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In another aspect, the present invention is refers to a process for obtaining a compound of formula (I) comprising:

to)

Dissolve a compound of formula (IV):

6

where R 1 -R 10, [A] m, j and k are defined as in claim 1, in anhydrous dimethylformamide.

b)

Add triethylamine and benzotriazol-1-yl-oxytripyrrolidinphosphonium hexafluorophosphate (PyBOP) at the dissolution of step (a) under inert conditions

C)

Dissolve a compound of formula (V)

7

in anhydrous dimethylformamide,

d)

Do react the solution obtained in step (b) with the solution obtained in step (c) at room temperature.

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In another aspect, the present invention also refers to pharmaceutical compositions comprising at least a compound of the invention, or a tautomer, a salt pharmaceutically acceptable, a derivative or a prodrug thereof, together with a pharmaceutically acceptable carrier or carrier, a excipient or a vehicle, for administration to a patient.

In a preferred embodiment, the composition Pharmaceutical also includes another active ingredient.

Pharmaceutical adjuvants and vehicles Acceptable that can be used in such compositions are the adjuvants and vehicles known to those skilled in the art and commonly used in the preparation of compositions therapeutic

In the sense used in this description, the expression "therapeutically effective amount" refers to the amount of agent or compound capable of carrying out the action therapeutic determined by its pharmacological properties, calculated to produce the desired effect and, in general, will come determined, among other causes, by the characteristics of the compounds, including the age, condition of the patient, the severity of the alteration or disorder, and of the route and frequency of administration.

The compounds described herein invention, its salts, prodrugs and / or solvates as well as the pharmaceutical compositions containing them can be used together with other drugs, or active ingredients, additional to Provide a combination therapy. Such additional drugs they can be part of the same pharmaceutical composition or, alternatively, they can be provided in the form of a separate composition for simultaneous administration or not to that of the pharmaceutical composition comprising a compound of formula (I), or a salt, prodrug or solvate thereof.

In another particular embodiment, said therapeutic composition is prepared in the form of a solid form or aqueous suspension, in a pharmaceutically acceptable diluent. The therapeutic composition provided by this invention may be administered by any appropriate route of administration, for which said composition will be formulated in the pharmaceutical form appropriate to the route of administration chosen. In one embodiment in particular, the administration of the therapeutic composition provided by this invention is performed orally, topically, rectal or parenteral (including subcutaneous, intraperitoneal, intradermal, intramuscular, intravenous, etc.).

In a preferred embodiment of the present invention, the pharmaceutical compositions are suitable for the oral administration, in solid or liquid form. The possible ways for oral administration are tablets, capsules, syrups or solutions and may contain conventional excipients known in the pharmaceutical field, as aggregating agents (e.g. syrup, acacia, gelatin, sorbitol, tragacanth or polyvinyl pyrrolidone), fillers (e.g. lactose, sugar, corn starch, calcium phosphate, sorbitol or glycine), disintegrants (e.g. starch, polyvinyl pyrrolidone or microcrystalline cellulose) or a surfactant Pharmaceutically acceptable as sodium lauryl sulfate.

Compositions for oral administration can be prepared by conventional methods of Pharmacy Galenic, as a mixture and dispersion. The tablets can be coated following methods known in the pharmaceutical industry.

Pharmaceutical compositions can be adapt for parenteral administration, as solutions sterile, suspensions, or lyophilized products of the invention, using the appropriate dose. Can be used suitable excipients, such as pH buffering agents or surfactants

The aforementioned formulations they can be prepared using conventional methods, such as described in the Pharmacopoeias of different countries and in other texts reference.

The administration of the compounds or Compositions of the present invention can be made by any suitable method, such as intravenous infusion and routes oral, intraperitoneal or intravenous. Oral administration is the preferred for patient convenience and for character Chronic diseases to be treated.

The administered amount of a compound of the The present invention will depend on the relative efficacy of the compound. chosen, the severity of the disease to be treated and the weight of the patient. However, the compounds of this invention will be administered one or more times a day, for example 1, 2, 3 or 4 times daily, with a total dose between 0.1 and 1000 mg / kg / day. Is important to keep in mind that it may be necessary to introduce dose variations, depending on age and condition of the patient, as well as modifications in the route of administration.

The compounds and compositions herein invention can be used together with other medications in Combined therapies The other drugs may be part of the same or different composition, for your administration at the same time or at different times.

In another aspect the present invention relates at the use of at least one compound of formula (I) for manufacturing of a medicine.

In another aspect the present invention relates at the use of at least one compound of formula (I) for manufacturing of a medication for the treatment of a cognitive disorder that is selected from senile dementia, cerebrovascular dementia, slight alteration of knowledge, deficit disorders attention, neurodegenerative dementia diseases associated with aberrant protein aggregations such as disease Alzheimer, amyotrophic lateral sclerosis, prion diseases such as Creutzfeldt-Jakob disease or Gerstmann-Straussler-Scheinker, Parkinson's disease, polyglutamine disease, tauopathies such as Pick's disease, frontotemporal dementia, paralysis progressive supranuclear or systemic amyloidosis.

Preferably, the cognitive disorder is the Alzheimer disease.

The use of the compounds of the invention is compatible with its use in protocols in which the compounds of the formula (I), or mixtures thereof are used by themselves or in combinations with other treatments or any medical procedure.

In a final aspect, the present invention is refers to the use of a compound of formula (I) as a reagent in biological tests

Throughout the description and the claims the word "comprises" and its variants not they intend to exclude other technical characteristics, additives, components or steps. For those skilled in the art, other objects, advantages and features of the invention will be partly detached of the description and in part of the practice of the invention. The The following examples are provided by way of illustration, and are not It is intended to be limiting of the present invention.

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Examples Example 1 Synthesis of the compounds of the invention

Scheme 1 shows the procedure for preparation of the compounds of the invention of general formula I, when the connector contains an amide group. Other procedures of synthesis of compounds with amine, ether or ester type bonds are Obvious to an experienced chemist.

Scheme one

8

For example, to a corresponding solution derivative of bis (aralkyl) amine (0.5 mmol) in anhydrous dimethylformamide (DMF, 8 mL), triethylamine (1.2 mmoles), hexafluorophosphate benzotriazol-1-yl-oxitripyrrolidinophosphonio (PyBOP, 0.6 mmol), and the corresponding amino derivative (0.5 mmoles), under an inert atmosphere. The mixture was stirred at temperature. overnight, and then the DMF evaporated to dryness under reduced pressure. The residue was dissolved in CH2Cl2 (10 mL) and washed consecutively with an aqueous acid solution citric (10%, 3x10 mL), an aqueous solution of NaHCO3 (10% 3x10 mL) and water (3x10 mL). The organic phase was dried over Na 2 SO 4, the solvent was completely removed under reduced pressure

The reaction products can be purified by conventional methods, such as crystallization or chromatography. When the process before described leads to mixtures of isomers, these can be separated by conventional techniques such as preparative chromatography. At case of stereogenic centers the compounds can be obtained in racemic form or pure enantiomers can be prepared by stereospecific synthesis or by resolution.

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Example 1.1

N - (2- (1 H -Indol-3-yl) ethyl) -4 - ((benzyl (methyl) amino) methyl) benzamide

Pale yellow solid. Yield: 46%. Mp: 94-96 ° C. MS (HEI): m / e = 398 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.20 (s, NH), 7.65 (s, 1H), 7.62 (d, 2H, J = 8.2), 7.37 ( d, 2H, J = 8.2), 7.33 (m, 5H), 7.25 (m, 1H), 7.21 (td, 1H, J = 7.1, J = 1.0), 7.12 (td, 1H, J = 7.9, J = 1.0), 7.05 (m, 1H), 6.22 (t, NH, J = 5.4), 3.79 (q, 2H, J = 6.6), 3.53 (s, 2H), 3.51 (s, 2H), 3.09 (t, 2H, J = 6.6), 2.16 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.4 (CONH), 142.9 (C), 138.8 (C), 136.4 (C), 133.4 (C), 128.9 ( 2CH), 128.9 (2CH), 128.3 (2CH), 127.3 (C), 127.1 (CH), 126.8 (2CH), 122.2 (CH), 122.1 (CH), 119.5 (CH), 118.7 (CH), 113.0 ( C), 111.3 (CH), 61.8 (CH 2), 61.3 (CH 2), 42.2 (CH 3), 40.2 (CH 2), 25.3 (CH 2). Analysis (%), calculated for C 26 H 27 N 3 O: C, 78.56, H, 6.85, N, 10.57; Found: C, 78.95, H, 7.20, N, 10.48.

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Example 1.2

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-hydroxy-1 H -indole-3-yl) ethyl) benzamide

Solid white. Yield: 30%. Mp: 75-77 ° C. MS (HEI): m / e = 414 [M + H] +. 1 H-NMR (500 MHz, CD 3 OD), δ (ppm): 7.74 (d, 2H, J = 8.2), 7.41 (d, 2H, J = 8.2), 7.32 (m, 4H), 7.24 (t, 1H, J = 7.1), 7.15 (d, 1H, J = 8.5), 7.03 (s, 1H), 6.99 (d, 1H, J = 2.2), 6.66 (dd, 1H, J = 8.5, J = 2.2), 3.63 (d, 2H, J = 7.4), 3.54 (s, 2H), 3.51 (s, 2H), 2.98 (t, 2H, J = 7.4), 2.16 (s, 3H) . 13 C-NMR (500 MHz, CD 3 OD), δ (ppm): 170.6 (CONH), 151.7 (C), 144.2 (C), 140.1 (C), 135.3 (C), 133.6 (C), 130.8 (2CH), 130.7 (2CH), 130.0 (C), 129.8 (2CH), 128.8 (CH), 128.7 (2CH), 124.7 (CH), 113.2 (CH), 113.1 (C), 112.9 (CH), 104.1 (CH), 63.3 (CH2), 62.7 (CH2), 42.9 (CH3), 42.5 (CH2), 26.8 (CH2) Analysis ( %), calculated for C 2 6H 27 N 3 O 2: C, 75.52, H, 6.58, N, 10.16; Found: C, 75.12, H, 6.37, N, 9.93.

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Example 1.3

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide

Pale yellow solid. Yield: 72%. Mp: 102-103 ° C. MS (HEI): m / e = 428 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.68 (s, NH), 7.66 (d, 2H, J = 8.2), 7.36 (d, 2H, J = 8.2 ), 7.32 (m, 4H), 7.20 (d, 1H, J = 8.8), 7.04 (d, 1H, J = 2.3), 6.95 (d, 1H, J = 2.3), 6.84 (dd, 1H, J = 8.8, J = 2.3), 6.52 (t, NH, J = 5.5), 3.75 (q, 2H, J = 6.7), 3.74 (s, 3H), 3.51 (s, 4H), 3.03 (t, 2H, J = 6.7), 2.16 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.4 (CONH), 153.8 (C), 143.0 (C), 138.8 (C), 133.1 (C), 131.5 ( C), 128.8 (2CH), 128.8 (2CH), 128.2 (2CH), 127.6 (C), 127.0 (CH), 126.8 (2CH), 123.0 (CH), 112.3 (C), 112.2 (CH), 112.1 ( CH), 100.2 (CH), 61.7 (CH 2), 61.1 (CH 2), 55.6 (CH 3), 42.1 (CH 3), 40.4 (CH 2), 25.2 ( CH2). Analysis (%), calculated for C 27 H 29 N 3 O 2: C, 75.85, H, 6.84, N, 9.83; Found: C, 76.13, H, 7.12, N, 10.15.

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Example 1.4

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide

Pale yellow solid. Yield: 65%. Mp: 119-121 ° C. MS (HEI): m / e = 428 [M + H] +. 1 H-NMR (400 MHz, acetone-d6), δ (ppm): 7.87 (d, 2H, J = 8.2), 7.49 (d, 1H, J = 8.6), 7.44 (d, 2H, J = 8.2), 7.37 (m, 2H), 7.31 (m, 2H), 7.23 (m, 1H), 7.05 (s, 1H), 6.90 (d, 1H, J = 2.2), 6.68 (dd, 1H, J = 8.6, J = 2.2), 3.76 (s, 3H), 3.69 (t, 2H, J = 7.4), 3.54 (s, 2H), 3.51 (s, 2H), 3.02 (t, 2H, J = 7.4 ), 2.12 (s, 3H). 13 C-NMR (400 MHz, acetone-d 6), δ (ppm): 167.2 (CONH), 157.2 (C), 143.7 (C), 140.2 (C), 138.2 (C), 134.7 (C), 129.5 (2CH), 129.3 (2CH), 129.0 (2CH), 127.9 (2CH), 127.7 (CH), 122.9 (C), 121.7 (CH), 119.9 (CH), 113.4 (C), 109.6 (CH), 95.2 (CH), 62.3 (CH 2), 61.9 (CH 2), 55.6 (CH 3), 42.3 (CH 3), 41.2 (CH 2), 26.3 (CH2). Analysis (%), calculated for C 27 H 29 N 3 O 2: C, 75.85, H, 6.84, N, 9.83; Found: C, 75.77, H, 6.95, N, 9.78.

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Example 1.5

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-fluoro-1 H -indole-3-yl) ethyl) benzamide

Pale yellow solid. Yield: 65%. Mp: 83-85 ° C. MS (HEI): m / e = 416 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.63 (s, NH), 7.63 (d, 2H, J = 8.2), 7.49 (dd, 1H, J = 9.0 , J = 5.2), 7.36 (d, 2H, J = 8.2), 7.32 (m, 4H), 7.24 (m, 1H), 6.99 (dd, 1H, J = 9.7, J = 2.3), 6.96 (d, 1H, J = 2.3), 6.84 (td, 1H, J = 9.0, J = 2.3), 6.40 (t, 1H, J = 5.7), 3.74 (q, 2H, J = 6.7), 3.24 (s, 2H) , 3.51 (s, 2H), 3.02 (t, 2H, J = 6.7), 2.16 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.5 (CONH), 161.1 (C), 158.8 (C), 143.0 (C), 138.7 (C), 136.3 ( C, J = 12.2), 128.9 (2CH), 128.8 (2CH), 128.3 (2CH), 127.1 (CH), 126.8 (2CH), 123.9 (C), 122.4 (CH), 122.3 (CH), 119.4 (C ), 119.3 (CH), 112.8 (CH), 108.0 (CH, J = 24.4), 97.6 (CH, J = 25.9), 61.8 (CH 2), 61.2 (CH 2), 42.1 (CH_ { 3}), 40.2 (CH 3), 25.2 (CH 2). Analysis (%), calculated for C 26 H 26 FN 3 O: C, 75.16, H, 6.31, N, 10.11; Found: C, 74.83, H, 5.96, N, 9.86.

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Example 1.6

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-chlorobenzyl) (methyl) amino) methyl) benzamide

Solid white. Yield: 49%. Mp: 97-99 ° C. MS (HEI): m / e = 432 [M + H] +. 1 H-NMR (500 MHz, CDCl 3), δ (ppm): 8.20 (s, NH), 7.65 (d, 1H, J = 8.1), 7.62 (d, 2H, J = 8.3 ), 7.52 (dd, 1H, J = 7.6, J = 1.2), 7.38 (d, 2H, J = 8.3), 7.37 (d, 1H, J = 8.1), 7.34 (dd, 1H, J = 7.8, J = 1.0) .7.21 (m, 4H), 7.13 (t, 1H, J = 7.2), 7.06 (d, J = 2.0), 6.23 (t, NH, J = 5.4), 3.80 (q, 2H, J = 6.6), 3.63 (s, 2H), 3.60 (s, 2H), 3.09 (t, 2H, J = 6.6), 2.19 (s, 3H). 13 C-NMR (500 MHz, CDCl 3), δ (ppm): 167.3 (CONH), 143.0 (C), 136.5 (C), 136.4 (C), 134.2 (C), 133.4 ( C), 130.6 (CH), 129.4 (CH), 128.9 (2CH), 128.1 (CH), 127.2 (CH), 126.8 (2CH), 126.6 (CH), 122.2 (CH), 122.1 (CH), 119.5 ( CH), 118.7 (CH), 113.0 (C), 111.3 (CH), 61.7 (CH 2), 58.5 (CH 2), 42.2 (CH 3), 40.2 (CH 2), 25.3 (CH2). Analysis (%), calculated for C 26 H 26 ClN 3 O: C, 72.29, H, 6.07, N, 9.73; Found: C, 72.42, H, 6.11, N, 9.77.

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Example 1.7

4 - (((2-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide

Solid white. Yield: 57%. Mp: 120-122 ° C. MS (HEI): m / e = 462 [M + H] +. 1 H-NMR (500 MHz, CD 3 OD), δ (ppm): 7.73 (d, 2H, J = 8.3), 7.54 (d, 1H, J = 8.3), 7.46 (d, 1H, J = 8.3), 7.44 (d, 2H, J = 8.6), 7.32 (d, 1H, J = 8.3), 7.36 (m; 1H), 7.21 (d, J = 8.8), 6.72 (dd, 1H , J = 8.8, J = 2.4), 3.72 (s, 3H), 3.66 (s, 2H), 3.63 (s, 2H), 3.34 (t, 2H, J = 7.3), 3.03 (t, 2H, J = 7.3), 2.20 (s, 3H). 13 C-NMR (500 MHz, CD 3 OD), δ (ppm): 170.1 (CONH), 154.9 (C), 137.5 (C), 135.5 (C), 134.8 (C), 133.3 (C), 132.3 (C), 132.3 (CH), 130.5 (CH), 130.4 (CH), 130.3 (C), 130.2 (2CH), 129.7 (CH), 129.2 (C), 128.3 (2CH), 113.3 (C), 112.9 (CH), 112.7 (CH), 101.2 (CH), 62.6 (CH 2), 59.4 (CH 2), 56.1 (CH 3), 42.5 (CH 2) , 42.3 (CH 3), 26.3 (CH 2). Analysis (%), calculated for C 27 H 28 ClN 3 O 2: C, 70.19, H, 6.11, N, 9.10; Found: C, 70.42, H, 6.31, N, 9.43.

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Example 1.8

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-chlorobenzyl) (methyl) amino) methyl) benzamide

Solid white. Yield: 55%. Mp: 110-112 ° C. MS (HEI): m / e = 432 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.21 (s, NH), 7.64 (d, 2H, J = 8.3), 7.64 (m, 1H), 7.37 ( d, 2H, J = 8.3), 7.37 (m, 2H), 7.23 (m, 4H), 7.13 (td, 1H, J = 7.5, J = 1.0), 7.06 (d, 1H, J = 2.5), 6.23 (t, NH, J = 5.5), 3.80 (q, 2H, J = 6.7), 3.52 (s, 2H), 3.46 (s, 2H), 3.09 (t, 2H, J = 6.7), 2.16 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.4 (CONH), 142.8 (C), 141.3 (C), 136.4 (C), 134.2 (C), 133.5 ( C), 129.5 (CH), 128.8 (2CH), 128.7 (2CH), 127.3 (C), 127.2 (CH), 126.9 (2CH), 122.2 (CH), 122.1 (CH), 119.5 (CH), 118.7 ( CH), 113.0 (C), 111.3 (CH), 61.4 (CH 2), 61.2 (CH 2), 42.3 (CH 3), 40.2 (CH 2), 25.3 (CH 2) }). Analysis (%), calculated for C 26 H 26 ClN 3 O: C, 72.29, H, 6.07, N, 9.73; Found: C, 72.31, H, 6.13, N, 9.81.

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Example 1.9

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide

Pale yellow solid. Yield: 82%. Mp: 108-110 ° C. MS (HEI): m / e = 462 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.15 (s, NH), 7.62 (d, 2H, J = 8.3), 7.35 (d, 2H, J = 8.3 ), 7.34 (s, 2H), 7.25 (d, 1H, J = 8.6), 7.22 (m, 3H), 7.03 (s. 2H), 6.85 (dd, 1H, J = 8.6, J = 2.3), 6.27 (t, NH, J = 5.6), 3.77 (q, 2H, J = 6.5), 3.86 (s, 3H), 3.51 (s, 2H) 3.45 (s, 2H), 3.04 (t, 2H, J = 6.5 ), 2.14 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.6 (CONH), 154.3 (C), 143.1 (C), 141.5 (C), 134.4 (C), 133.6 ( C), 131.7 (C), 129.8 (CH), 129.1 (2CH), 129.0 (2CH), 127.9 (C), 127.5 (CH), 127.2 (2CH), 123.2 (CH), 113.0 (C), 112.8 ( CH), 112.3 (CH), 100.5 (CH), 61.7 (CH 2), 61.4 (CH 2), 56.0 (CH 3), 42.5 (CH 3), 40.5 (CH 2) }), 25.5 (CH2). Analysis (%), calculated for C 27 H 28 ClN 3 O 2: C, 70.19, H, 6.11, N, 9.10; Found: C, 70.11, H, 6.09, N, 9.31.

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Example 1.10

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide

Pale yellow solid. Yield: 82%. Mp: 58-60 ° C. MS (HEI): m / e = 462 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.31 (s, NH), 7.65 (d, 2H, J = 8.4), 7.48 (d, 1H, J = 8.6 ), 7.36 (d, 2H, J = 8.4), 7.35 (s, 1H), 7.23 (m, 3H), 6.91 (d, 1H, J = 2.2), 6.84 (d, 1H, J = 2.2), 6.78 (dd, 1H, J = 8.6, J = 2.2), 6.35 (t, NH, J = 5.6), 3.81 (s, 3H), 3.75 (q, 2H, J = 6.6), 3.52 (s, 2H) 3.46 (s, 2H), 3.03 (t, 2H, J = 6.6), 2.15 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.4 (CONH), 156.5 (C), 142.7 (C), 141.2 (C), 137.2 (C), 134.1 ( C), 133.4 (C), 129.85 (CH), 128.8 (2CH), 128.7 (2CH), 127.2 (CH), 126.9 (2CH), 121.6 (C), 120.9 (CH), 119.3 (C), 112.7 ( C), 109.4 (CH), 94.7 (CH), 61.4 (CH 2), 61.2 (CH 2), 55.6 (CH 3), 42.2 (CH 3), 40.2 (CH 2) }), 25.3 (CH2). Analysis (%), calculated for C 27 H 28 ClN 3 O 2: C, 70.19, H, 6.11, N, 9.10; Found: C, 70.06, H, 6.13, N, 9.23.

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Example 1.11

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide

Solid white. Yield: 61%. Mp: 80-82 ° C. MS (HEI): m / e = 428 [M + H] +. 1 H-NMR (500 MHz, CD 3 OD), δ (ppm): 7.74 (d, 2H, J = 8.3), 7.60 (d, 1H, J = 8.1), 7.42 (d, 2H, J = 8.3), 7.31 (m, 2H), 7.24 (td, 1H, J = 7.8, J = 1.7), 7.09 (s, 1H), 7.06 (m, 1H), 6.97 (td, 1H, J = 8.1, J = 1.0), 6.93 (m, 1H), 6.91 (td, 1H, J = 7.6, J = 1.0), 3.78 (s, 3H), 3.66 (t, 2H, J = 7.4), 3.54 ( s, 2H) 3.34 (s, 2H), 3.06 (t, 2H, J = 7.4), 2.18 (s, 3H). 13 C-NMR (500 MHz, CD 3 OD), δ (ppm): 170.1 (CONH), 159.4 (C), 143.7 (C), 138.1 (C), 134.7 (C), 132.1 (CH), 130.4 (2CH), 129.7 (CH), 128.8 (C), 128.2 (2CH), 127.0 (C), 123.4 (CH), 122.3 (CH), 121.2 (CH), 119.6 (CH), 119.4 (CH), 113.4 (C), 112.2 (CH), 111.6 (CH), 62.5 (CH 2), 56.0 (CH 3), 55.7 (CH 2), 42.6 (CH 3) , 42.2 (CH2), 26.3 (CH2). Analysis (%), calculated for C 27 H 29 N 3 O 3: C, 75.85, H, 6.84, N, 9.83; Found: C, 75.77, H, 6.81, N, 9.91.

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Example 1.12

N - (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide

Pale yellow solid. Yield: 76%. Mp: 58-60 ° C. MS (HEI): m / e = 458 [M + H] +. 1 H-NMR (400 MHz, CDCl 3), δ (ppm): 8.18 (s, NH), 7.62 (d, 2H, J = 8.6), 7.40 (m, 1H), 7.39 ( d, 2H, J = 8.6), 7.25 (d, 1H, J = 9.0), 7.22 (td, 1H, J = 7.7, J = 2.0), 7.03 (m, 2H), 6.94 (td, 1H, J = 7.7, J = 1.0), 6.85 (m, 2H), 6.27 (t, NH, J = 5.6), 3.79 (s, 3H), 3.77 (s, 3H), 3.75 (q, 2H, J = 6.6), 3.58 (s, 2H) 3.55 (s, 2H), 3.05 (t, 2H, J = 6.6), 2.20 (s, 3H). 13 C-NMR (400 MHz, CDCl 3), δ (ppm): 167.4 (CONH), 157.7 (C), 154.0 (C), 143.3 (C), 133.2 (C), 131.5 ( C), 130.3 (CH), 128.9 (2CH), 128.0 (CH), 127.7 (C), 126.8 (C), 126.7 (2CH), 122.9 (CH), 120.3 (CH), 112.8 (C), 112.5 ( CH), 112.0 (CH), 110.3 (CH), 100.3 (CH), 61.7 (CH 2), 55.8 (CH 2), 55.3 (CH 3), 55.2 (CH 3), 42.4 (CH 3), 40.3 (CH 2), 25.3 (CH 2). Analysis (%), calculated for C 28 H 31 N 3 O 3: C, 75.50, H, 6.83, N, 9.18; Found: C, 75.61, H, 6.79, N, 9.12.

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Example 1.13

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-methoxybenzyl) (methyl) amino) methyl) benzamide

Pale yellow solid. Yield: 78%. Mp: 81-83 ° C. MS (HEI): m / e = 428 [M + H] +. 1 H-NMR (400 MHz, CD 3 OD), δ (ppm): 7.74 (d, 2H, J = 8.4), 7.60 (dd, 1H, J = 8.1, J = 1.0), 7.42 (d, 2H, J = 8.4), 7.32 (dd, 1H, J = 8.1, J = 1.0), 7.22 (t, 1H, J = 8.1), 7.09 (s, IH), 7.06 (td, 1H, J = 8.1, J = 1.0), 6.98 (td, 1H, J = 8.1, J = 1.0), 6.93 (d, 1H, J = 2.0), 6.91 (d, 1H, J = 8.1), 6.80 (dd, 1H, J = 8.1, J = 2.0), 3.77 (s, 3H), 3.66 (t, 2H, J = 7.4), 3.53 (s, 2H) 3.48 (s, 2H), 3.05 (t, 2H, J = 7.4), 2.16 (s, 3H). 13 C-NMR (400 MHz, CD 3 OD), δ (ppm): 170.1 (CONH), 161.2 (C), 143.8 (C), 141.3 (C), 138.1 (C), 134.8 (C), 130.3 (CH), 130.3 (2CH), 128.8 (C), 128.3 (2CH), 123.4 (CH), 122.5 (CH), 122.3 (CH), 119.6 (CH), 119.4 (CH), 115.7 (CH), 113.7 (CH), 113.4 (C), 112.2 (CH), 62.7 (CH 2), 62.1 (CH 2), 55.6 (CH 3), 42.5 (CH 3) , 42.2 (CH2), 26.3 (CH2). Analysis (%), calculated for C 27 H 29 N 3 O 2: C, 75.85, H, 6.84, N, 9.83; Found: C, 75.66, H, 6.71, N, 9.81.

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Example 1.14

N - (2- (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((3-methoxybenzyl) (methyl) amino) methyl) benzamide

Solid white. Yield: 63%. Mp: 82-84 ° C. MS (HEI): m / e = 458 [M + H] +. 1 H-NMR (500 MHz, CD 3 OD), δ (ppm): 7.71 (d, 2H, J = 8.3), 7.38 (d, 2H, J = 8.3), 7.21 (d, 1H, J = 8.7), 7.20 (t, 1H, J = 7.6), 7.06 (d, 1H, J = 2.4), 7.05 (s, 1H), 6.90 (d, 1H, J = 1.2), 6.88 (d , 1H, J = 7.6), 6.78 (dd, 1H, J = 7.6, J = 1.2), 6.72 (dd, 1H, J = 8.7, J = 2.4), 3.75 (s, 3H), 3.70 (s, 3H ), 3.63 (t, 2H, J = 7.3), 3.49 (s, 2H) 3.44 (s, 2H), 3.02 (t, 2H, J = 7.3), 2.13 (s, 3H). 13 C-NMR (500 MHz, CD 3 OD), δ (ppm): 170.0 (CONH), 161.2 (C), 154.9 (C), 143.8 (C), 141.3 (C), 134.7 (C), 133.3 (C), 130.3 (CH), 130.2 (2CH), 129.2 (C), 128.3 (2CH), 124.2 (CH), 122.5 (CH), 115.6 (CH), 113.7 (CH), 113.3 (C), 112.9 (CH), 112.7 (CH), 101.2 (CH), 62.7 (CH 2), 62.1 (CH 2), 56.1 (CH 3), 55.6 (CH 3) , 42.5 (CH2), 42.3 (CH3), 26.3 (CH2). Analysis (%), calculated for C 28 H 31 N 3 O 3: C, 75.50, H, 6.83, N, 9.18; Found: C, 75.38, H, 6.78, N, 8.82.

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Example 2 Cell viability of the compounds of the invention

The cell viability of the compounds of the invention was measured in the human neuroblastoma cell line SH-SY5Y. The cells used were among passages 3 and 16 after defrosting and remained in DMEM medium (Dulbecco's modified Eagle's medium), containing 15 non-essential amino acids and supplemented with 10% fetal serum bovine, 1 mM glutamine, 50 U mL -1 penicillin, and 50 µg mL -1 of streptomycin (GIBCO, Madrid, Spain). The crops are planted in jars containing supplemented medium and they maintained at 37 ° C in humidified air with 5% dioxide carbon, making 1: 4 passes twice a week. For the assays, SH-SY5Y cells were cultured again in 48-well plates with a sowing density of 10 5 cells per well and were treated for 24 hours with the compounds of the invention at various concentrations. The Quantitative measurement of cell death was performed by measuring the percentage of the enzyme lactate dehydrogenase (LDH) released at extracellular medium (cytotoxicity detection kit, Roche). The LDH amount was evaluated using a microplate reader (Labsystems iMES Reader MF) at 492 nm (wavelength of excitation) and 620 nm (emission wavelength). Were used controls with 100% cell viability. At 1 µM all products have 100% cell viability, which indicates that the compounds of the present invention are not toxic and have a wide range of therapeutic safety.

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Example 3 Neuroprotective properties of the compounds of the invention against mitochondrial oxidative stress

Using the neuroblastoma cell line human SH-SY5Y, the cytoprotective action was evaluated of compounds against cell death caused by radicals mitochondrial free. The conditions of oxidative stress mitochondrial were simulated using a mixture of rotenone (30 µM) and oligomycin A (10 µM). The compounds were incubated with the cells for 24 hours, after which, the medium was replaced by a new one that contained the compound to be evaluated and the toxic agent, then incubated for 24 hours additional. Cellular survival was determined by measuring the LDH activity.

Extracellular and intracellular LDH activity was measured by UV-vis using a kit of cytotoxicity (Roche-Boehringer. Mannheim, Germany), following the manufacturer's instructions. Total LDH activity is defined as the sum of intra- and extracellular activity and the LDH activity released as the percentage of activity extracellular compared to total activity. The activity of the LDH released was calculated for each experiment considering as 100% extracellular LDH, released by the vehicle with respect to the total.

9

where subídices e, i refer to extracellular and intracellular, respectively.

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The calculation of the% protection of each of Derivatives were carried out as follows: in each individual experiment, the LDH obtained in untreated cells (baseline) was subtracted from LDH released after treatment with the 100% toxic and normalized. This value was subtracted from 100.

10

The results are expressed as the mean of at minus three independent crops, each containing four repetitions of each compound evaluated.

Melatonin was used as a reference, which has recognized neuroprotective properties against oxidative stress (Rosales-Corral, S. et al. J. Pineal Res . 2003, 35 , 80-84). The results are shown in table 1 and are the average of three independent experiments.

Neuroprotection results indicate that the compounds of the invention are capable of capturing radicals mitochondrial free and protect neurons from oxidative damage mitochondrial As a consequence, the compounds of this invention they are potential drugs for the treatment of pathologies or conditions related to oxidative stress or excessive mitochondrial free radical production.

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Example 4 Evaluation of the compounds of the invention as inhibitors of the human acetylcholinesterase enzyme (h-AChE)

Inhibition of human acetylcholinesterase (h-AChE) was performed following the method of Ellman (Ellman, GL et al. Biochem. Pharmacol . 1961, 7 , 88-95). The test solution was formed by 0.1 M phosphate buffer at pH = 8, 400 µM 5,5'-dithiobisnitrobenzoic acid (DTNB, Ellman reagent), 0.05 units / mL of the recombinant h-AChE enzyme (Sigma Chemical Co. ) and acetylthiocholine iodide (800 µM) as a substrate for the enzymatic reaction. The compounds to be evaluated were pre-incubated with the enzyme for 5 minutes at 30 ° C, the substrate was added and the absorbance changes were measured at 412 nm every 5 minutes in a 96-well UV-vis microplate reader (Multiskan Spectrum, Thermo Electron Co.). The reaction rates were compared and the percentages of inhibition due to the presence of the compounds being analyzed were calculated. The IC 50 value is defined as the concentration of compound that reduces enzymatic activity by 50%. Tacrine, a known h-AChE inhibitor was used as control of the quality of the assay. The results are found in table 1, expressed as the average of three independent experiments.

The results of inhibition of h-AChE indicate that the compounds of the invention are able to increase neurotransmitter levels and, therefore Therefore, they are able to increase cognitive abilities. How consequently, the compounds of the invention are potential drugs for the symptomatic treatment of the disease of Alzheimer's and related pathologies.

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Example 5 Site propidium iodide displacement test peripheral anionic of AChE by the compounds of the invention

In order to determine if the new compounds were able to bind to the peripheral anionic site (PAS) of the AChE, its experimental affinity for the PAS was studied by the displacement of propidium iodide. This salt is a ligand. specific to PAS, which when bound to the enzyme it presents a 10-fold increase in its signal of fluorescence, which makes it a very useful probe for evaluate whether a certain compound binds to the enzyme's PAS.

A solution of bovine AChE was used at a 5 µM concentration in Tris buffer (0.1 mm, Ph 8.0). They were added Aliquots of the products to be tested at concentrations of 0.3, 1.0 and 3.0 µM and the solutions were left at room temperature for 6 hours Then, the samples were incubated for 15 minutes with propidium iodide at a final concentration of 20 µM and fluorescence was measured in a microplate reader of 96-wells (Fluostar Optima, BMG, Germany). The excitation and emission wavelengths were 485 and 620 nm, respectively. The results are expressed as the average of three independent experiments and are found in table 1.

The displacement test results of propidium indicate that the compounds of the invention bind to the site peripheral anionic of the AChE and therefore would be able to inhibit the aggregation of Abeta promoted by this enzyme. How consequently, the compounds of this invention are potential drugs for the treatment of neurodegenerative diseases in those that produce aberrant protein aggregates, such as Alzheimer disease.

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TABLE 1 Neuroprotection (NP,%) against rotenone: oligomycin A (30:10 µM) using 1 µM of compound, inhibition of human acetylcholinesterase (h-AChE) such as IC 50 (µM), and displacement of propidium (%) from the site peripheral anionic (PAS) of AChE at three concentrations of compounds evaluated

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Example 6 Test to evaluate the ability of the compounds of the invention for the capture of oxygen radicals (method ORAC-FL)

The antioxidant capacity of the compounds of the invention was determined by competition with fluorescein in the capture of oxygen radicals following the ORAC-FL method, widely established and used in the food industry (Ou, B. et al. J. Agric Food Chem . 2001, 49 , 4619-4626; Dávalos, A. et al. J. Agric. Food Chem . 2004, 52 , 48-54).

The ORAC-FL experiments are carried out on a Polarstar Galaxy fluorimeter with reader 96-well plates (BMG Labtechnologies GMBH, Offenburg, Germany), with excitation and emission filters at 485-P and 520-P. The team was controlled by the Fluostar Galaxy software (version 4.11-0) for the measurement of fluorescence. The reagents 2,2'-azobis- (amidinopropane) dihydrochloride (AAPH), acid (±) -6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic (trolox), and fluorescein (FL) were acquired at Sigma-Aldrich. The reaction was performed in buffer phosphate (75 nM, pH 7.4) and the final reaction volume was 200 \ muL. A mixture of the compound to be evaluated (20 µL) and fluorescein (120 µL; 70 nM, final concentration) was put in a 96-well black microplate (Nunc). The mixture is pre-incubated for 15 minutes at 37 ° C and then the solution was added of AAPH (60 µL; 12 mM, final concentration) in a very Fast using a multichannel pipette. The microplate was introduced quickly in the reader and the fluorescence was measured every minute over a period of 80 minutes. Before each reading, it stirred automatically the microplate. The products were evaluated using eight different concentrations (0.1-1 µM). For the calibration of each test, eight were measured trolox solutions (1-8 µM), as well as a blank (FL + AAPH) in which phosphate buffer was used without any product. All reaction mixtures were prepared in duplicate, and at least three independent experiments were performed for each One of the samples to evaluate. The fluorescence curves in time function were normalized by subtracting the white curve corresponding to each trial, then calculating the area below the curve (AUC). The net AUC of each sample was calculated subtracting the AUC from the target to the AUC obtained for each of the samples. The representation of the net AUC against the concentration of compound provided straight lines, which were adjusted by linear regression. ORAC-FL values are expressed as? of trolox /? of compound (trolox equivalents) on a dimensionless scale, in which trolox is assigned the value ORAC-FL = 1. The results of table 2 are the mean ± SEM of three independent experiments.

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TABLE 2 Oxygen radical absorption capacity by fluorescence (ORAC-FL)

12

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The results of the ORAC experiments indicate that the compounds of the invention are between 1.5 and 4.3 times more potent that trolox (the active part of vitamin E) in the capture of oxygen radicals and, therefore, can decrease the damage Biological produced by these reactive species. Therefore, the Compounds of the invention are potential drugs for the treatment of pathologies or conditions related to excessive free radical production, as is the disease of Alzheimer's

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Example 7 In vitro evaluation of the penetration in the central nervous system of the compounds of the invention

The passage of the blood-brain barrier of the compounds of the invention was evaluated using a permeability test through an artificial membrane, PAMPA (Parallel Artificial Membrane Permeation Assay), following the procedure of Di et al ( Eur. J. Med. Chem . 2003, 38 , 223-232) which has been optimized in our laboratory for molecules of reduced aqueous solubility (Rodríguez-Franco, MI et al., J. Med. Chem . 2006, 49 , 459-462; Reviriego, F. et al., J. Am. Chem. Soc . 2006, 128 , 16458-16459; Pavón, FJ et al., Neuropharmacology 2006, 51 , 358-366; Marco-Contelles, J. et al., J. Med. Chem . 2009, 52 , 2724-2732; Camps, P. et al., J. Med. Chem . 2009, 52 , 5365-5379).

Commercial patterns, phosphate buffer saline at pH 7.4 (PBS) and dodecane were purchased from Sigma, Aldrich, Acros and Fluka. Millex filters (PVDF membrane, 25 mm diameter, pore size 0.45 µm) and microplates 96-wells were purchased from Millipore. He Pork Brain Lipid Extract (PBL) was purchased from Avanti Polar Lipids At the bottom of each of the 96 wells of the donor microplate there is a PVDF filter (pore size 0.45 um) and the wells of the acceptor plate are in the form of tear.

The receptor plate was filled with 170 µL of PBS: ethanol (9: 1) and the donor plate filter surface was impregnated with 4 µL of PBL in dodecane (20 mg mL -1). The compounds to be evaluated were dissolved in PBS: ethanol (9: 1) at 1 mg mL -1, filtered through a Millex filter and then added to the donor wells (170 µL). The donor plate was carefully placed on the acceptor for 240 minutes at 25 ° C. After incubation, the donor plate was removed and the concentration of each of the products in the acceptor plate was determined by UV-vis. Each sample was analyzed at five wavelengths in four wells and at least in three independent experiments. The results are expressed as mean ± standard deviation. In each experiment, 17 commercial drugs of which their degree of penetration into the CNS were known: testosterone, verapamil, imipramine, desipramine, astemizole, progesterone, promazine, chloropromazine, clonidine, corticosterone, piroxicam, hydrocortisone, caffeine, aldosterone, Lomefloxacin, enoxacin, ofloxacin. According to the permeability described, the experimental values of P e (10 6 cm s -1) were between 11.1 ± 0.1 (testosterone) and 0.2 ± 0.01 (ofloxacin). The results obtained for the compounds of the invention are in Table 3 and are the mean ± SD of three independent experiments.

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TABLE 3 Permeability ( P e, 10-6 cm s -1) of the compounds in the PAMPA-BBB assay and prediction of their penetration into the CNS

13

a Compounds with high blood brain barrier permeability (cns +): P e> 2 10 - 6 cm s -1

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An optimal penetration into the CNS is a essential property that drugs designed for the treatment of pathologies or conditions that affect the brain, such as It is Alzheimer's disease. Test results PAMPA-BBB indicate that the compounds of the invention they would be able to penetrate the CNS and therefore, they would be able of reaching its therapeutic targets located in the brain.

Claims (27)

1. A compound of formula (I) 14 where

R_ {a} R 15 are independently selected from hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted), aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), COR_ {a}, C (O) OR_ {a}, C (O) NR_ {a} R_ {b}, C = NR_a, CN, OR_ {a}, OC (O) R_ {a}, S (O) r -R_ {a}, NR_ {a} R_ {b}, NR_ {a} C (O) R_ {b}, NO_ {2}, N = CR_ {a} R_ {b} or halogen;

R_ {a} and R b are independently selected from hydrogen, alkyl (substituted or unsubstituted), cycloalkyl (substituted or unsubstituted), alkoxy (substituted or unsubstituted), alkenyl (substituted or unsubstituted), aryl (substituted or unsubstituted), heteroaryl (substituted or unsubstituted), or halogen, with the proviso that they are not halogens when they are attached to an N.

r is selected between 0, 1 or 2.

j and k are numbers that are independently selected between 1 and 8.

A, B, D and E se independently select between CR_ {a} R_ {b}, CR_ {a} = CR_ {b}, CO, O, S, or NR_ {a}; where R_ {a} and R_ {b} are define as above;

m, n, p, and what are numbers that are independently selected from a value between 0 and 10, provided that its sum is at least four, m + n + p + q \ geq 4

X and Y know independently select between CH or N; Z is selected from CH, O, S or N, with the proviso that at least one of X, Y or Z is a heteroatom;

when Z is C or N, then R 11 is selected from hydrogen, alkyl substituted or unsubstituted, substituted or unsubstituted cycloalkyl, substituted or unsubstituted alkenyl, substituted or unsubstituted aryl substituted, substituted or unsubstituted heterocycle, alkoxy substituted or unsubstituted or aryloxy substituted or not replaced;

when Z is O or S, then R_ {11} does not exist.

the spacer [A] m - [B] n - [D] p - [E] q It is suitable as a substituent in X or Y when X or Y are C, the which are C instead of CH.

or an isomer, a pharmaceutically salt acceptable, a pro-drug or a solvate of same.

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2. Compound according to claim 1 wherein R 3 -R 5 and R 7 -R 10 they are H. 3. Compound according to any of the claims 1 or 2 wherein R 6 is alkyl C_ {1} -C_ {4}. 4. Compound according to claim 3 wherein R 6 is CH 3. 5. Compound according to any of the claims 1 to 4 where j and k are 1. 6. Compound according to any of the claims 1 to 5 wherein R 1 and R 2 are selected independently between hydrogen, halogen or alkoxy.

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7. Compound of formula (II) according to claim 1: fifteen

where R_ {1}, R 2, R 6, R 11 -R 15, A, B, D, E, m, n, p, q, W, X, Y and Z are defined as in the claim one.

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8. Compound according to claim 7 wherein one of X and Y is C and the other is CH. 9. Compound according to claim 8 wherein Z it's N. 10. Compound according to claim 9 wherein R 11, R 12 and R 15 are H. 11. Compound of formula (III) according to claim 1: 16

where R_ {1}, R 2, R 6, R 13, R 14, A, B, D, E, m, n, p, q defined as in claim 1.

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12. Compound according to claim 11 wherein R 13 and R 14 are independently selected from H, halogen, OH, or alkoxy. 13. Compound according to any of the claims 1 to 12 wherein m + n + p + q is a value that is selected between 4, 5, 6, 7, 8, 9, or 10. 14. Compound according to claim 13 wherein m + n + p + q is 4. 15. Compound according to claim 13 wherein the spacer [A] m - [B] n - [D] p - [E] q is selected among the formulas (CH 2) r CO-NR a - (CH 2) s -, - (CH 2) r -NR a -CO- (CH 2) s -, (CH 2) r -CO-O- (CH 2) s -, - (CH 2) r -O-CO- (CH 2) s -, wherein R_a is defined as in claim 1; r and s se independently select a value between 0 and 8. 16. Compound according to claim 15 wherein the spacer has the formula - (CH 2) r -CO-NR a - (CH 2) s -. 17. Compound according to claim 16 wherein r is 0 and s is 2. 18. Compound according to claim 17 wherein R_ {a} is H. 19. Compound selected from the group that consists in:

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - ((benzyl (methyl) amino) methyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-hydroxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - ((Benzyl (methyl) amino) methyl) - N - (2- (6-fluoro-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-chlorobenzyl) (methyl) amino) methyl) benzamide;

?

4 - (((2-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-chlorobenzyl) (methyl) amino) methyl) benzamide;

?

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (5-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

4 - (((3-Chlorobenzyl) (methyl) amino) methyl) - N - (2- (6-methoxy-1 H -indole-3-yl) ethyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((2-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (1 H -Indol-3-yl) ethyl) -4 - (((3-methoxybenzyl) (methyl) amino) methyl) benzamide;

?

N - (2- (5-Methoxy-1 H -indole-3-yl) ethyl) -4 - (((3-methoxybenzyl) (methyl) amino) methyl) benzamide;

or an isomer, a pharmaceutically salt acceptable, a prodrug or a solvate thereof.

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20. Procedure for obtaining a compound of formula (I) comprising:

to)

Dissolve a compound of formula (IV):

17

where R 1 -R 10, [A] m, j and k are defined as in claim 1, in anhydrous dimethylformamide.

b)

Add triethylamine and benzotriazol-1-yl-oxytripyrrolidinphosphonium hexafluorophosphate (PyBOP) at the dissolution of step (a) under inert conditions

C)

Dissolve a compound of formula (V)

18

in anhydrous dimethylformamide,

d)

Do react the solution obtained in step (b) with the solution obtained in step (c) at room temperature.

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21. Pharmaceutical composition comprising a compound of formula (I) according to any one of claims 1 to 19 and a pharmaceutically active carrier, adjuvant or vehicle acceptable. 22. Composition according to claim 21 which It also includes another active substance. 23. Use of at least one compound of formula (I) according to any of claims 1 to 19 for manufacturing of a medicine. 24. Use of at least one compound of formula (I) according to any of claims 1 to 19 for manufacturing of a medication for the treatment of a cognitive disorder that is selected from senile dementia, cerebrovascular dementia, slight alteration of knowledge, deficit disorders attention, neurodegenerative dementia diseases associated with aberrant protein aggregations such as disease Alzheimer, amyotrophic lateral sclerosis, prion diseases such as Creutzfeldt-Jakob disease or Gerstmann-Straussler-Scheinker, Parkinson's disease, polyglutamine disease, tauopathies such as Pick's disease, frontotemporal dementia, paralysis progressive supranuclear or systemic amyloidosis. 25. Use according to claim 24 wherein the Cognitive disorder is Alzheimer's disease. 26. Use according to any of the claims 24 or 25 for oral administration. 27. Use of a compound according to any of the claims 1 to 19 as reagent in biological assays.