ES2239004T3 - Purificacion de polihidroxialcanoatos. - Google Patents

Purificacion de polihidroxialcanoatos.

Info

Publication number: ES2239004T3
Authority: ES; Spain
Prior art keywords: pha; biomass; solvent; organic solvent; water
Prior art date: 1999-05-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

ES00930657T

Other languages

English (en)

Spanish (es)

Inventor

Daniel Horowitz

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Yield10 Bioscience Inc

Original Assignee

Metabolix Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-05-12

Filing date

2000-05-12

Publication date

2005-09-16

2000-05-12 Application filed by Metabolix Inc filed Critical Metabolix Inc

2005-09-16 Application granted granted Critical

2005-09-16 Publication of ES2239004T3 publication Critical patent/ES2239004T3/es

2020-05-12 Anticipated expiration legal-status Critical

Status Expired - Lifetime legal-status Critical Current

Links

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239000000203 mixture Substances 0.000 claims abstract description 15
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239000003153 chemical reaction reagent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
239000012141 concentrate Substances 0.000 description 1
OXBLHERUFWYNTN-UHFFFAOYSA-M copper(I) chloride Chemical compound [Cu]Cl OXBLHERUFWYNTN-UHFFFAOYSA-M 0.000 description 1
235000005822 corn Nutrition 0.000 description 1
150000003997 cyclic ketones Chemical class 0.000 description 1
HPXRVTGHNJAIIH-UHFFFAOYSA-N cyclohexanol Chemical compound OC1CCCCC1 HPXRVTGHNJAIIH-UHFFFAOYSA-N 0.000 description 1
GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical class CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 description 1
229940120124 dichloroacetate Drugs 0.000 description 1
JXTHNDFMNIQAHM-UHFFFAOYSA-N dichloroacetic acid Chemical compound OC(=O)C(Cl)Cl JXTHNDFMNIQAHM-UHFFFAOYSA-N 0.000 description 1
239000003085 diluting agent Substances 0.000 description 1
XTDYIOOONNVFMA-UHFFFAOYSA-N dimethyl pentanedioate Chemical compound COC(=O)CCCC(=O)OC XTDYIOOONNVFMA-UHFFFAOYSA-N 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
235000013399 edible fruits Nutrition 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
XYIBRDXRRQCHLP-UHFFFAOYSA-N ethyl acetoacetate Chemical compound CCOC(=O)CC(C)=O XYIBRDXRRQCHLP-UHFFFAOYSA-N 0.000 description 1
229940116333 ethyl lactate Drugs 0.000 description 1
241001233957 eudicotyledons Species 0.000 description 1
230000008713 feedback mechanism Effects 0.000 description 1
235000004426 flaxseed Nutrition 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
239000007789 gas Substances 0.000 description 1
125000001475 halogen functional group Chemical group 0.000 description 1
FUZZWVXGSFPDMH-UHFFFAOYSA-N hexanoic acid Chemical compound CCCCCC(O)=O FUZZWVXGSFPDMH-UHFFFAOYSA-N 0.000 description 1
229920001519 homopolymer Polymers 0.000 description 1
150000002430 hydrocarbons Chemical class 0.000 description 1
239000001257 hydrogen Substances 0.000 description 1
125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
230000002209 hydrophobic effect Effects 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
238000011081 inoculation Methods 0.000 description 1
GJRQTCIYDGXPES-UHFFFAOYSA-N iso-butyl acetate Natural products CC(C)COC(C)=O GJRQTCIYDGXPES-UHFFFAOYSA-N 0.000 description 1
229940117955 isoamyl acetate Drugs 0.000 description 1
229940035429 isobutyl alcohol Drugs 0.000 description 1
FGKJLKRYENPLQH-UHFFFAOYSA-M isocaproate Chemical compound CC(C)CCC([O-])=O FGKJLKRYENPLQH-UHFFFAOYSA-M 0.000 description 1
OQAGVSWESNCJJT-UHFFFAOYSA-N isovaleric acid methyl ester Natural products COC(=O)CC(C)C OQAGVSWESNCJJT-UHFFFAOYSA-N 0.000 description 1
150000002576 ketones Chemical class 0.000 description 1
150000002632 lipids Chemical class 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
230000002503 metabolic effect Effects 0.000 description 1
125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
230000004048 modification Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 1
HNBDRPTVWVGKBR-UHFFFAOYSA-N n-pentanoic acid methyl ester Natural products CCCCC(=O)OC HNBDRPTVWVGKBR-UHFFFAOYSA-N 0.000 description 1
235000016709 nutrition Nutrition 0.000 description 1
230000035764 nutrition Effects 0.000 description 1
QNGNSVIICDLXHT-UHFFFAOYSA-N para-ethylbenzaldehyde Natural products CCC1=CC=C(C=O)C=C1 QNGNSVIICDLXHT-UHFFFAOYSA-N 0.000 description 1
235000020232 peanut Nutrition 0.000 description 1
TWSRVQVEYJNFKQ-UHFFFAOYSA-N pentyl propanoate Chemical compound CCCCCOC(=O)CC TWSRVQVEYJNFKQ-UHFFFAOYSA-N 0.000 description 1
WVDDGKGOMKODPV-ZQBYOMGUSA-N phenyl(114C)methanol Chemical compound O[14CH2]C1=CC=CC=C1 WVDDGKGOMKODPV-ZQBYOMGUSA-N 0.000 description 1
229920003023 plastic Polymers 0.000 description 1
239000004033 plastic Substances 0.000 description 1
229920001155 polypropylene Polymers 0.000 description 1
239000000047 product Substances 0.000 description 1
230000000750 progressive effect Effects 0.000 description 1
235000013772 propylene glycol Nutrition 0.000 description 1
AOHJOMMDDJHIJH-UHFFFAOYSA-N propylenediamine Chemical compound CC(N)CN AOHJOMMDDJHIJH-UHFFFAOYSA-N 0.000 description 1
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 1
230000004044 response Effects 0.000 description 1
230000000717 retained effect Effects 0.000 description 1
238000000638 solvent extraction Methods 0.000 description 1
229910001220 stainless steel Inorganic materials 0.000 description 1
239000010935 stainless steel Substances 0.000 description 1
239000000126 substance Substances 0.000 description 1
229960004793 sucrose Drugs 0.000 description 1
235000020238 sunflower seed Nutrition 0.000 description 1
239000004094 surface-active agent Substances 0.000 description 1
229920001897 terpolymer Polymers 0.000 description 1
YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 description 1
BSYVTEYKTMYBMK-UHFFFAOYSA-N tetrahydrofurfuryl alcohol Chemical compound OCC1CCCO1 BSYVTEYKTMYBMK-UHFFFAOYSA-N 0.000 description 1
PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 description 1
229940117972 triolein Drugs 0.000 description 1
238000000108 ultra-filtration Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/88—Post-polymerisation treatment
- C08G63/90—Purification; Drying
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/88—Post-polymerisation treatment
- C08G63/89—Recovery of the polymer
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/62—Carboxylic acid esters
- C12P7/625—Polyesters of hydroxy carboxylic acids
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G63/00—Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
- C08G63/02—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds
- C08G63/06—Polyesters derived from hydroxycarboxylic acids or from polycarboxylic acids and polyhydroxy compounds derived from hydroxycarboxylic acids

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicinal Chemistry (AREA)
Polymers & Plastics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
General Chemical & Material Sciences (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Biological Depolymerization Polymers (AREA)
Polyesters Or Polycarbonates (AREA)

ES00930657T 1999-05-12 2000-05-12 Purificacion de polihidroxialcanoatos. Expired - Lifetime ES2239004T3 (es)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US13374799P	1999-05-12	1999-05-12
US133747P		1999-05-12

Publications (1)

Publication Number	Publication Date
ES2239004T3 true ES2239004T3 (es)	2005-09-16

Family

ID=22460122

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
ES00930657T Expired - Lifetime ES2239004T3 (es)	1999-05-12	2000-05-12	Purificacion de polihidroxialcanoatos.

Country Status (9)

Country	Link
US (2)	US6340580B1 (fr)
EP (1)	EP1175499B1 (fr)
JP (1)	JP4790912B2 (fr)
AT (1)	ATE292188T1 (fr)
AU (1)	AU750835B2 (fr)
CA (1)	CA2373131C (fr)
DE (1)	DE60019098T2 (fr)
ES (1)	ES2239004T3 (fr)
WO (1)	WO2000068409A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE10013514C2 (de) *	2000-03-14	2002-06-27	Ufz Leipzighalle Gmbh	Verfahren zur Gewinnung von Polyhydroxyalkanoaten (PHA) oder deren Copolymeren
GB2373743B (en) *	2001-03-27	2004-11-03	Membrane Extraction Tech Ltd	Solvent exchange process
CA2494322C (fr) *	2002-08-06	2012-05-22	Metabolix Inc.	Procedes d'extraction de polymeres
DE10247157A1 (de) *	2002-10-09	2004-04-22	Messo-Chemietechnik Gmbh	Formgebung und Wäsche gelbildender Biopolymere
ES2297385T3 (es) *	2003-02-21	2008-05-01	Metabolix, Inc.	Composiciones adhesivas de pha.
US7781539B2 (en) *	2003-02-21	2010-08-24	Metabolix Inc.	PHA blends
TW200613560A (en) *	2004-06-29	2006-05-01	Procter & Gamble	Solvent extraction of polyhydroxyalkanoates from biomass
TW200617171A (en) *	2004-06-29	2006-06-01	Procter & Gamble	Improved process for the solvent-based extraction of polyhydroxyalkanoates from biomass
AU2005285271B2 (en) *	2004-09-13	2010-09-09	Metabolix, Inc.	Single solvent polymer extraction methods
US20060057691A1 (en) *	2004-09-15	2006-03-16	Karunakaran Narasimhan	Process for the extraction of polyhydroxyalkanoates from biomass
US7118897B2 (en) *	2004-09-15	2006-10-10	The Procter & Gamble Company	Process for the extraction of polyhydroxyalkanoates from biomass
JPWO2008026619A1 (ja) *	2006-08-30	2010-01-21	株式会社カネカ	３−ヒドロキシアルカン酸共重合体の製造方法
US7514525B2 (en) *	2007-03-09	2009-04-07	University Of Hawaii	Recovery and purification of polyhydroxyalkanoates
BR112013016851B1 (pt)	2011-01-12	2021-08-31	Intervet International B.V	Método para preparar uma composição farmacêutica que compreende partículas de uma droga
ES2448823B1 (es)	2012-08-14	2014-10-13	Neol Biosolutions, S.A.	Producción de bioplásticos
CN109054254B (zh)	2012-08-17	2021-02-02	Cj第一制糖株式会社	用于聚合物共混物的生物基橡胶改性剂
EP3004225A1 (fr)	2013-05-30	2016-04-13	Metabolix, Inc.	Mélanges de recyclat
EP3122817B1 (fr)	2014-03-27	2020-07-29	CJ CheilJedang Corporation	Systèmes polymères fortement chargés
CN109173422B (zh) *	2018-10-09	2021-03-09	飞潮（无锡）过滤技术有限公司	一种医药生产中转晶前工序api药液的批次完全过滤工艺

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3061384D1 (en) *	1979-02-21	1983-01-27	Ici Plc	A process for the extraction of poly-3-hydroxy-butyric acid from microbial cells
US4336334A (en) *	1979-02-21	1982-06-22	Imperial Chemical Industries Limited	Microbiological process for the production of poly(β-hydroxybutyric acid)
FR2567149B1 (fr) *	1984-07-06	1986-12-05	Solvay	Procede pour l'extraction de poly-beta-hydroxybutyrates au moyen d'un solvant a partir d'une suspension aqueuse de micro-organismes
GB8927794D0 (en) *	1989-12-08	1990-02-14	Ici Plc	Copolymer production
US5610041A (en) *	1991-07-19	1997-03-11	Board Of Trustees Operating Michigan State University	Processes for producing polyhydroxybutyrate and related polyhydroxyalkanoates in the plastids of higher plants
US5281691A (en) *	1992-06-19	1994-01-25	Eastman Kodak Company	Poly(3-hydroxyalkanoates)
EP0846184B1 (fr) *	1995-08-21	2002-05-22	The Procter & Gamble Company	Extraction par solvant de polyhydroxyalcanoates (pha) de la biomasse, facilitee par l'utilisation d'un non-solvant marginal pour pha
US5942597A (en) *	1995-08-21	1999-08-24	The Procter & Gamble Company	Solvent extraction of polyhydroxyalkanoates from biomass
US6083729A (en) *	1995-10-26	2000-07-04	Metabolix, Inc.	Methods for isolating polyhydroxyalkanoates from plants
US5821299A (en) *	1996-02-16	1998-10-13	The Proctor & Gamble Company	Solvent extraction of polyhydroxy-alkanoates from biomass facilitated by the use of marginal nonsolvent
WO1998046783A1 (fr) *	1997-04-15	1998-10-22	Monsanto Company	Extraction de pha a haute temperature au moyen de solvants pauvres en pha
US6043063A (en) *	1997-04-15	2000-03-28	Monsanto Company	Methods of PHA extraction and recovery using non-halogenated solvents
US6156852A (en) *	1997-04-21	2000-12-05	Monsanto Company	Hydroxy-terminated polyhydroxyalkanoates

2000
- 2000-05-12 AU AU48440/00A patent/AU750835B2/en not_active Ceased
- 2000-05-12 WO PCT/US2000/013039 patent/WO2000068409A1/fr not_active Ceased
- 2000-05-12 ES ES00930657T patent/ES2239004T3/es not_active Expired - Lifetime
- 2000-05-12 EP EP00930657A patent/EP1175499B1/fr not_active Expired - Lifetime
- 2000-05-12 JP JP2000616375A patent/JP4790912B2/ja not_active Expired - Fee Related
- 2000-05-12 DE DE60019098T patent/DE60019098T2/de not_active Expired - Lifetime
- 2000-05-12 US US09/570,261 patent/US6340580B1/en not_active Expired - Lifetime
- 2000-05-12 CA CA002373131A patent/CA2373131C/fr not_active Expired - Fee Related
- 2000-05-12 AT AT00930657T patent/ATE292188T1/de not_active IP Right Cessation
2002
- 2002-01-18 US US10/052,434 patent/US20020058316A1/en not_active Abandoned

Also Published As

Publication number	Publication date
JP2002543794A (ja)	2002-12-24
DE60019098T2 (de)	2006-02-02
WO2000068409A1 (fr)	2000-11-16
AU4844000A (en)	2000-11-21
EP1175499B1 (fr)	2005-03-30
US6340580B1 (en)	2002-01-22
CA2373131C (fr)	2004-11-02
US20020058316A1 (en)	2002-05-16
AU750835B2 (en)	2002-08-01
JP4790912B2 (ja)	2011-10-12
ATE292188T1 (de)	2005-04-15
CA2373131A1 (fr)	2000-11-16
DE60019098D1 (de)	2005-05-04
EP1175499A1 (fr)	2002-01-30

Publication	Publication Date	Title
ES2239004T3 (es)	2005-09-16	Purificacion de polihidroxialcanoatos.
US6043063A (en)	2000-03-28	Methods of PHA extraction and recovery using non-halogenated solvents
US9045595B2 (en)	2015-06-02	Process for recovering polyhydroxialkanoates (“PHAs”) from cellular biomass
EP0973930B1 (fr)	2007-06-13	Extraction de pha a haute temperature a l' aide de faibles solvants a pha
CN101035902B (zh)	2012-07-04	脂肪醇作为可塑剂在用于改善phb及其共聚物的物理-机械性质和加工性中的用途
ES2384630T3 (es)	2012-07-10	Procedimiento para el aislamiento de polihidroxialcanoatos (PHA) de c�lulas bacterianas mediante extracci�n en disolvente
EP1070135B1 (fr)	2010-02-24	Procedes pour la separation et la purification des biopolymeres
US7226765B2 (en)	2007-06-05	Solvent extraction of polyhydroxyalkanoates from biomass
WO2001068890A2 (fr)	2001-09-20	Prevention de la gelification de solutions de polyhydroxyalkanoate par le cisaillement
CN1926239A (zh)	2007-03-07	聚羟基链烷酸酯的制造方法
JP6692232B2 (ja)	2020-05-13	３ｈｂの製造方法
WO1994026917A1 (fr)	1994-11-24	Purification de polyester
BRPI0405622B1 (pt)	2019-08-06	PROCESSO PARA RECUPERAÇÃO DE POLIHIDROXIALCANOATOS (PHAs) DE BIOMASSA CELULAR