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ES2221488T3 - Metodo y sistema para detectar oligonucleotidos en una muestra. - Google Patents

Metodo y sistema para detectar oligonucleotidos en una muestra.

Info

Publication number
ES2221488T3
ES2221488T3 ES99973075T ES99973075T ES2221488T3 ES 2221488 T3 ES2221488 T3 ES 2221488T3 ES 99973075 T ES99973075 T ES 99973075T ES 99973075 T ES99973075 T ES 99973075T ES 2221488 T3 ES2221488 T3 ES 2221488T3
Authority
ES
Spain
Prior art keywords
oligonucleotides
sample
target
verification
sensing interface
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES99973075T
Other languages
English (en)
Inventor
Itamar Willner
Dea Amos Bar
Fernando Patolsky
Evgeny Katz
Arie Dagan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Yissum Research Development Co of Hebrew University of Jerusalem
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from IL12734698A external-priority patent/IL127346A/en
Application filed by Yissum Research Development Co of Hebrew University of Jerusalem filed Critical Yissum Research Development Co of Hebrew University of Jerusalem
Application granted granted Critical
Publication of ES2221488T3 publication Critical patent/ES2221488T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)

Abstract

Un método para detectar un oligonucleótido diana en una muestra que comprende: (a) proporcionar un dispositivo de detección que tenga una interfaz de detección que contenga oligonucleótidos, teniendo cada uno una secuencia de nucleótidos complementaria al menos en una porción de hibridación estable de la misma a una primera porción de los oligonucleótidos diana, donde dicho dispositivo de detección comprende una sonda electroquímica que lleva la interfaz de detección; (b) proporcionar oligonucleótidos de verificación, teniendo cada uno una secuencia de nucleótidos complementaria al menos en una porción de hibridación estable de la misma a una segunda porción del oligonucleótido diana, distinta de la primera porción; (c) poner en contacto la muestra con la interfaz de detección en condiciones que permitan que los oligonucleótidos diana, si están presentes en la mezcla, hibriden para dar los oligonucleótidos de captura; (d) antes de (c) o después, permitir que los oligonucleótidos de verificación hibridan para dar los oligonucleótidos diana si están presentes en la muestra; y (e) detectar la presencia de dichos oligonucleótidos de verificación en la interfaz de detección.
ES99973075T 1998-12-01 1999-12-01 Metodo y sistema para detectar oligonucleotidos en una muestra. Expired - Lifetime ES2221488T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL12734698A IL127346A (en) 1998-12-01 1998-12-01 Method and system for detecting oligonucleotides in a sample
IL13296699A IL132966A0 (en) 1998-12-01 1999-11-16 Method and system for detecting oligonucleotides in a sample

Publications (1)

Publication Number Publication Date
ES2221488T3 true ES2221488T3 (es) 2004-12-16

Family

ID=26323752

Family Applications (1)

Application Number Title Priority Date Filing Date
ES99973075T Expired - Lifetime ES2221488T3 (es) 1998-12-01 1999-12-01 Metodo y sistema para detectar oligonucleotidos en una muestra.

Country Status (9)

Country Link
EP (1) EP1135533B1 (es)
JP (1) JP2002531100A (es)
AT (1) ATE264923T1 (es)
AU (1) AU770025B2 (es)
CA (1) CA2353562A1 (es)
DE (1) DE69916650T2 (es)
ES (1) ES2221488T3 (es)
IL (1) IL132966A0 (es)
WO (1) WO2000032813A1 (es)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1409728A2 (en) * 2001-04-09 2004-04-21 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Non-enzymatic liposome-linked closely spaced array electrodes assay (nel-ela) for detecting and quantifying nucleic acids
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
JP2006514264A (ja) * 2002-05-08 2006-04-27 イサム リサーチ デベロップメント カンパニー オブ ザ ヘブルー ユニバーシティ オブ エルサレム 液体溶媒中の検体の測定
EP1445609B1 (en) 2002-08-23 2006-11-22 Kabushiki Kaisha Toshiba Base sequencing electrode, base sequencing device and base sequencing method
DE10256898B4 (de) * 2002-11-29 2006-01-12 Senslab-Gesellschaft Zur Entwicklung Und Herstellung Bioelektrochemischer Sensoren Mbh Elektrochemischer Nachweis von Nukleinsäuren
CA2522750A1 (en) * 2003-01-06 2004-07-22 Bioinvent International Ab Immobilisation of dna-labelled lipid vesicles on dna arrays
JPWO2006033400A1 (ja) * 2004-09-22 2008-05-15 ダイキン工業株式会社 Ｄｎａ検出方法及びレポータプローブ
JP4561451B2 (ja) * 2005-04-15 2010-10-13 セイコーエプソン株式会社 多型検出方法および多型検出用キット
JP4561478B2 (ja) * 2005-05-26 2010-10-13 セイコーエプソン株式会社 多型検出方法、多型検出用キット
DE102006003603B4 (de) * 2006-01-25 2010-02-04 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vernetzbare multifunktionelle Träger für (niedermolekulare) Liganden und deren Anwendung in der Analytik sowie Verfahren zu deren Herstellung und Vernetzung
WO2008095493A1 (en) * 2007-02-06 2008-08-14 Atonomics A/S Reporter unit for detection of target molecules using polymerisable substrate
CN101241097B (zh) * 2007-09-18 2011-08-03 中国科学院上海应用物理研究所 一种采用茎环结构检测探针的电化学dna检测方法及其试剂盒
DE102010000906B4 (de) * 2009-01-22 2012-07-12 RUHR-UNIVERSITäT BOCHUM Konjugate für den Nachweis doppelsträngiger Nukleinsäuren
JP2013513790A (ja) * 2009-12-09 2013-04-22 アイティーアイ・スコットランド・リミテッド 検体の検出
CN104698043A (zh) * 2013-12-09 2015-06-10 重庆医科大学 检测大肠杆菌的dna电化学生物传感器及其制备方法
WO2019026517A1 (ja) * 2017-07-31 2019-02-07 国立大学法人大阪大学 溶液中に存在する対象物質の検出方法および検出するためのシステム
CN111044580B (zh) * 2019-12-20 2021-10-01 中国农业大学 一种快速实时监测淡水鱼腌制程度的方法
JP2023119143A (ja) * 2022-02-16 2023-08-28 株式会社エヌエフホールディングス 被験検体中の目的核酸配列を測定するための測定装置および測定方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5695926A (en) * 1990-06-11 1997-12-09 Bio Merieux Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support
CA2095611A1 (en) * 1990-11-14 1992-05-15 Jennifer Kyoko Ishii Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor

Also Published As

Publication number Publication date
EP1135533B1 (en) 2004-04-21
DE69916650T2 (de) 2005-04-21
EP1135533A1 (en) 2001-09-26
DE69916650D1 (de) 2004-05-27
ATE264923T1 (de) 2004-05-15
WO2000032813A1 (en) 2000-06-08
AU1407400A (en) 2000-06-19
AU770025B2 (en) 2004-02-12
CA2353562A1 (en) 2000-06-08
JP2002531100A (ja) 2002-09-24
IL132966A0 (en) 2001-03-19

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