ES2139761T3 - Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union. - Google Patents
Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union.Info
- Publication number
- ES2139761T3 ES2139761T3 ES94931655T ES94931655T ES2139761T3 ES 2139761 T3 ES2139761 T3 ES 2139761T3 ES 94931655 T ES94931655 T ES 94931655T ES 94931655 T ES94931655 T ES 94931655T ES 2139761 T3 ES2139761 T3 ES 2139761T3
- Authority
- ES
- Spain
- Prior art keywords
- polypeptide
- binding
- ligand
- molecules
- polypeptides
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 9
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 9
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 9
- 230000027455 binding Effects 0.000 abstract 5
- 239000003446 ligand Substances 0.000 abstract 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract 2
- 102000000584 Calmodulin Human genes 0.000 abstract 2
- 108010041952 Calmodulin Proteins 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 229910052791 calcium Inorganic materials 0.000 abstract 2
- 239000011575 calcium Substances 0.000 abstract 2
- 230000001419 dependent effect Effects 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000010494 dissociation reaction Methods 0.000 abstract 1
- 230000005593 dissociations Effects 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 238000002372 labelling Methods 0.000 abstract 1
- MASXKPLGZRMBJF-MVSGICTGSA-N mastoparan Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(N)=O MASXKPLGZRMBJF-MVSGICTGSA-N 0.000 abstract 1
- 108010019084 mastoparan Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 230000009870 specific binding Effects 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4728—Calcium binding proteins, e.g. calmodulin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43563—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
- C07K14/43568—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from wasps
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Insects & Arthropods (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biotechnology (AREA)
- Food Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
Abstract
LAS MOLECULAS COMPRENDEN: I) UN POLIPEPTIDO (TAL COMO CALMODULINA) QUE TIENE UNA AFINIDAD DE UNION DEPENDIENTE DEL CALCIO PARA UN LIGANTE Y II) OTRO POLIPEPTIDO, LOS POLIPEPTIDOS ESTAN PREFERIBLEMENTE UNIDOS POR UN ENLACE DE PEPTIDO Y SE PRODUCEN MEDIANTE EXPRESION RECOMBINANTE A PARTIR DE UNA FUSION DE GENES. LAS MOLECULAS SON UTILES EN LA DETECCION, INMOVILIZACION, OBJETIVACION Y PURIFICACION, ETIQUETADO DE CELULAS, Y ENSAYOS DE DESPLAZAMIENTO DE BANDA PARA DETERMINAR LA UNION DE UN MIEMBRO DE UN PAR DE UNION ESPECIFICO (SBP) PARA UN MIEMBRO SBP COMPLEMENTARIO. PARA PROPOSITOS DE ENSAYOS DE DESPLAZAMIENTO DE BANDA EL POLIPEPTIDO I) NO NECESITA TENER AFINIDAD DE UNION DEPENDIENTE DEL CALCIO PARA UN LIGANTE, PERO DEBERIA TENER UNA DISOCIACION CONSTANTE PARA UN LIGANTE DE 10 NM O MENOR, MEDIDA A UN PH DE ENTRE 6 Y 9, A 20 (GRADOS) C. EN UNA CONFORMACION ALTERNATIVA, UN POLIPEPTIDO DE UNION DE LA CALMODULINA QUE ES, O SE DERIVA DEL METASPORAN, SE UNE, COMO POLIPEPTIDO I) EN VEZ DE UN POLIPEPTIDO DE UNION, AL OTRO POLIPEPTIDO.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB939322772A GB9322772D0 (en) | 1993-11-04 | 1993-11-04 | Tagging |
| GB9405927A GB9405927D0 (en) | 1993-11-04 | 1994-03-25 | Tagging |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2139761T3 true ES2139761T3 (es) | 2000-02-16 |
Family
ID=26303796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES94931655T Expired - Lifetime ES2139761T3 (es) | 1993-11-04 | 1994-11-04 | Produccion y utilizacion de polipeptidos marcados por medio de moleculas de union. |
Country Status (10)
| Country | Link |
|---|---|
| EP (1) | EP0726951B1 (es) |
| JP (1) | JPH09504694A (es) |
| AT (1) | ATE185373T1 (es) |
| AU (1) | AU695107B2 (es) |
| CA (1) | CA2175209A1 (es) |
| DE (1) | DE69421086T2 (es) |
| DK (1) | DK0726951T3 (es) |
| ES (1) | ES2139761T3 (es) |
| GR (1) | GR3031943T3 (es) |
| WO (1) | WO1995012672A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2667197A (en) * | 1996-04-16 | 1997-11-07 | University Of Miami | Stabilized preparations of human troponins and modifications thereof, diagnostic assay methods and assay kits |
| GB9608540D0 (en) * | 1996-04-25 | 1996-07-03 | Medical Res Council | Isolation of enzymes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5747334A (en) * | 1990-02-15 | 1998-05-05 | The University Of North Carolina At Chapel Hill | Random peptide library |
-
1994
- 1994-11-04 DE DE69421086T patent/DE69421086T2/de not_active Expired - Fee Related
- 1994-11-04 DK DK94931655T patent/DK0726951T3/da active
- 1994-11-04 WO PCT/GB1994/002420 patent/WO1995012672A1/en not_active Ceased
- 1994-11-04 JP JP7513097A patent/JPH09504694A/ja active Pending
- 1994-11-04 ES ES94931655T patent/ES2139761T3/es not_active Expired - Lifetime
- 1994-11-04 EP EP94931655A patent/EP0726951B1/en not_active Expired - Lifetime
- 1994-11-04 AU AU80654/94A patent/AU695107B2/en not_active Ceased
- 1994-11-04 CA CA002175209A patent/CA2175209A1/en not_active Abandoned
- 1994-11-04 AT AT94931655T patent/ATE185373T1/de not_active IP Right Cessation
-
1999
- 1999-11-25 GR GR990403035T patent/GR3031943T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0726951B1 (en) | 1999-10-06 |
| AU8065494A (en) | 1995-05-23 |
| CA2175209A1 (en) | 1995-05-11 |
| DE69421086D1 (de) | 1999-11-11 |
| EP0726951A1 (en) | 1996-08-21 |
| ATE185373T1 (de) | 1999-10-15 |
| WO1995012672A1 (en) | 1995-05-11 |
| JPH09504694A (ja) | 1997-05-13 |
| DE69421086T2 (de) | 2000-03-02 |
| AU695107B2 (en) | 1998-08-06 |
| GR3031943T3 (en) | 2000-03-31 |
| DK0726951T3 (da) | 2000-03-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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