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ES2114504B1 - METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. - Google Patents

METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT.

Info

Publication number
ES2114504B1
ES2114504B1 ES9601945A ES9601945A ES2114504B1 ES 2114504 B1 ES2114504 B1 ES 2114504B1 ES 9601945 A ES9601945 A ES 9601945A ES 9601945 A ES9601945 A ES 9601945A ES 2114504 B1 ES2114504 B1 ES 2114504B1
Authority
ES
Spain
Prior art keywords
solid support
deoxyoligonucleotides
enzymatic synthesis
synthesis
transferase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES9601945A
Other languages
Spanish (es)
Other versions
ES2114504A1 (en
Inventor
Cabrera Vicente Martinez
Infante Carlos Flores
Rojo Marta Izquierdo
Ferrer Mariano Hernandez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad Autonoma de Madrid
Original Assignee
Universidad Autonoma de Madrid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad Autonoma de Madrid filed Critical Universidad Autonoma de Madrid
Priority to ES9601945A priority Critical patent/ES2114504B1/en
Publication of ES2114504A1 publication Critical patent/ES2114504A1/en
Application granted granted Critical
Publication of ES2114504B1 publication Critical patent/ES2114504B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

METODO PARA LA SINTESIS ENZIMATICA DE DESOXIOLIGONUCLEOTIDOS EN SOPORTE SOLIDO, QUE CONSISTE EN REALIZAR LA SINTESIS SOBRE UN SOPORTE SOLIDO EN LA DIRECCION (5'') A (3''), UTILIZANDO LA TRANSFERASA TERMINAL, UNA ADN POLIMERASA INDEPENDIENTE DE MOLDE QUE PREFERENTEMENTE, CATALIZA LA INCORPORACION DE DESOXIRRIBONUCLEOSIDOS 5" TRIFOSFATOS A TERMINALES 3", HIDROXILO DE ADN MONOCATENARIO Y SE EMPLEA DESOXIRRIBONUCLEOTIDOS CON SU HIDROXILO (3'') TRANSITORIAMENTE PROTEGIDO CON OBJETO DE AÑADIR UN SOLO NUCLEOTIDO EN CADA CICLO.METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT, WHICH CONSISTS OF PERFORMING THE SYNTHESIS ON A SOLID SUPPORT IN THE DIRECTION (5 '') TO (3 ''), USING THE TERMINAL TRANSFERASE, INDEPENDENT MANDATORY TRANSFERASE, CATALYSIS THE INCORPORATION OF 5 "TRIPHOSPHATES TO 3" TERMINALS, HYDROXYL OF MONOCATENARY DNA AND DEOXYRIBONUCLEOTIDES ARE USED WITH ITS TRANSITIONALLY PROTECTED NUCLEAR CANDIDATE WITH A 3-YEAR OBJECT.

ES9601945A 1996-09-13 1996-09-13 METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT. Expired - Fee Related ES2114504B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES9601945A ES2114504B1 (en) 1996-09-13 1996-09-13 METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9601945A ES2114504B1 (en) 1996-09-13 1996-09-13 METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT.

Publications (2)

Publication Number Publication Date
ES2114504A1 ES2114504A1 (en) 1998-05-16
ES2114504B1 true ES2114504B1 (en) 1999-02-01

Family

ID=8296028

Family Applications (1)

Application Number Title Priority Date Filing Date
ES9601945A Expired - Fee Related ES2114504B1 (en) 1996-09-13 1996-09-13 METHOD FOR THE ENZYMATIC SYNTHESIS OF DEOXYOLIGONUCLEOTIDES IN SOLID SUPPORT.

Country Status (1)

Country Link
ES (1) ES2114504B1 (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3301833A1 (en) * 1983-01-20 1984-07-26 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig METHOD FOR SIMULTANEOUS SYNTHESIS OF SEVERAL OLIGONOCLEOTIDES IN A SOLID PHASE
US4605735A (en) * 1983-02-14 1986-08-12 Wakunaga Seiyaku Kabushiki Kaisha Oligonucleotide derivatives
US4816571A (en) * 1987-06-04 1989-03-28 Applied Biosystems, Inc. Chemical capping by phosphitylation during oligonucleotide synthesis
SE9003743D0 (en) * 1990-11-26 1990-11-26 Pharmacia Ab METHOD AND MEANS FOR OLIGONUCLEOTIDE SYNTHESIS
US5872244A (en) * 1994-09-02 1999-02-16 Andrew C. Hiatt 3' protected nucleotides for enzyme catalyzed template-independent creation of phosphodiester bonds

Also Published As

Publication number Publication date
ES2114504A1 (en) 1998-05-16

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