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ES2101706T3 - Procedimiento para la deteccion, especifica para un genero y para una especie, de bacterias en un liquido de muestra. - Google Patents

Procedimiento para la deteccion, especifica para un genero y para una especie, de bacterias en un liquido de muestra.

Info

Publication number
ES2101706T3
ES2101706T3 ES91116745T ES91116745T ES2101706T3 ES 2101706 T3 ES2101706 T3 ES 2101706T3 ES 91116745 T ES91116745 T ES 91116745T ES 91116745 T ES91116745 T ES 91116745T ES 2101706 T3 ES2101706 T3 ES 2101706T3
Authority
ES
Spain
Prior art keywords
species
bacteria
specific
genus
rna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES91116745T
Other languages
English (en)
Inventor
Wolfgang Ludwig
Karl-Heinz Schleifer
Christoph Kessler
Ruediger Rueger
Anne Stern
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Application granted granted Critical
Publication of ES2101706T3 publication Critical patent/ES2101706T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PARA LA DETENCCION ESPECIFICA DEL GENERO Y/O DE LA ESPECIE DE BACTERIAS EN UN LIQUIDO DE MUESTRA SE HIBRIDIZA RNA BACTERIANO CON UNA IMPRIMACION, QUE ES GENERALMENTE COMPLEMENTARIA DE UNA REGION ESPECIFICA DEL GENERO O DE LA ESPECIE DE RNA DE DETERMINADAS BACTERIAS O DE UNA REGION ALTAMENTE CONSERVADA DE LA RNA DE BACTERIAS, PERO EN SU EXTREMO-3'' NO ES COMPLEMENTARIA DE LA RNA DE BACTERIAS DE OTRA FAMILIA O ESPECIE, SE ALARGA LA IMPRIMACION EN PRESENCIA DE UNA POLIMERASA APROPIADA Y LOS CUATRO DESOXIRRIBONUCLEOTIDOS, DADO EL CASO CON MARCACION SIMULTANEA O SIGUIENTE DEL PRODUCTO DE ALARGAMIENTO, SE HIBRIDIZA UN PRODUCTO DE ALARGAMIENTO FORMADO DESPUES DE LA DESNATURALIZACION CON UN OLIGONUCLEOTIDO ESPECIFICO DEL GENERO O DE LA ESPECIE Y SE COMPRUEBA LA HIBRIDACION SOBRE LA MARCA DEL OLIGONUCLEOTIDO.
ES91116745T 1990-10-09 1991-10-01 Procedimiento para la deteccion, especifica para un genero y para una especie, de bacterias en un liquido de muestra. Expired - Lifetime ES2101706T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4032024 1990-10-09
DE4038804A DE4038804A1 (de) 1990-10-09 1990-12-05 Verfahren zur genus- oder/und spezies-spezifischen detektion von bakterien in einer probenfluessigkeit

Publications (1)

Publication Number Publication Date
ES2101706T3 true ES2101706T3 (es) 1997-07-16

Family

ID=25897574

Family Applications (1)

Application Number Title Priority Date Filing Date
ES91116745T Expired - Lifetime ES2101706T3 (es) 1990-10-09 1991-10-01 Procedimiento para la deteccion, especifica para un genero y para una especie, de bacterias en un liquido de muestra.

Country Status (7)

Country Link
US (1) US6225094B1 (es)
EP (1) EP0480289B1 (es)
JP (1) JPH0640839B2 (es)
AT (1) ATE150092T1 (es)
CA (1) CA2052668C (es)
DE (2) DE4038804A1 (es)
ES (1) ES2101706T3 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6001564A (en) * 1994-09-12 1999-12-14 Infectio Diagnostic, Inc. Species specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial pathogens and associated antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
FR2730234B1 (fr) * 1995-02-08 1997-03-28 Bio Merieux Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques
US5994079A (en) * 1998-02-06 1999-11-30 Digene Corporation Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function
US7399589B2 (en) 1998-02-06 2008-07-15 Digene Corporation Immunological detection of RNA:DNA hybrids on microarrays
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
ATE541947T1 (de) * 1999-05-14 2012-02-15 Entpr Ie Trd As Bioresearch Ie Diagnosetests auf basis einer nukleinsäuresonde für prokaryotische und eukaryotische organismen
AU5371099A (en) * 1999-07-22 2001-02-13 Artus Gesellschaft Fur Molekularbiologische Diagnostik Und Entwicklung Mbh Method for the species-specific detection of organisms
US20050050101A1 (en) * 2003-01-23 2005-03-03 Vockley Joseph G. Identification and use of informative sequences
US20090317798A1 (en) * 2005-06-02 2009-12-24 Heid Christian A Analysis using microfluidic partitioning devices
US9840732B2 (en) 2012-05-21 2017-12-12 Fluidigm Corporation Single-particle analysis of particle populations

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
FI883482A7 (fi) * 1986-11-24 1988-07-22 Gen Probe Inc Nukleiinihappokoettimia ei-virusperäisten organismien toteamista ja/ta i kvantitatiivista määritystä varten.
AU622104B2 (en) * 1987-03-11 1992-04-02 Sangtec Molecular Diagnostics Ab Method of assaying of nucleic acids, a reagent combination and kit therefore
JP2823566B2 (ja) * 1987-08-14 1998-11-11 アンスティテュ パストゥール バクテリア診断プローブ
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
EP0333465B1 (en) 1988-03-18 1994-07-13 Baylor College Of Medicine Mutation detection by competitive oligonucleotide priming
CA2012982A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid
US5232829A (en) * 1989-09-29 1993-08-03 Hoffmann-La Roche Inc. Detection of chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes

Also Published As

Publication number Publication date
DE4038804A1 (de) 1992-04-16
ATE150092T1 (de) 1997-03-15
CA2052668C (en) 2000-12-19
DE59108610D1 (de) 1997-04-17
US6225094B1 (en) 2001-05-01
JPH04258299A (ja) 1992-09-14
CA2052668A1 (en) 1992-04-10
EP0480289A1 (de) 1992-04-15
JPH0640839B2 (ja) 1994-06-01
EP0480289B1 (de) 1997-03-12

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