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ES2193873B1 - Metodo para la deteccion de presencia bacteriana en un sujeto. - Google Patents

Metodo para la deteccion de presencia bacteriana en un sujeto.

Info

Publication number
ES2193873B1
ES2193873B1 ES200200677A ES200200677A ES2193873B1 ES 2193873 B1 ES2193873 B1 ES 2193873B1 ES 200200677 A ES200200677 A ES 200200677A ES 200200677 A ES200200677 A ES 200200677A ES 2193873 B1 ES2193873 B1 ES 2193873B1
Authority
ES
Spain
Prior art keywords
subject
sample
target sequence
bacterial
detection
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES200200677A
Other languages
English (en)
Other versions
ES2193873A1 (es
Inventor
Carlos Muñoz Ruiz
Ruben Frances Guarinos
Jose Francisco Such Ronda
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to ES200200677A priority Critical patent/ES2193873B1/es
Publication of ES2193873A1 publication Critical patent/ES2193873A1/es
Application granted granted Critical
Publication of ES2193873B1 publication Critical patent/ES2193873B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

El método comprende (i) recoger una muestra biológica de un sujeto; (ii) tratar dicha muestra bajo condiciones que permiten poner en contacto el ADN contenido en dicha muestra que eventualmente comprende ADN bacteriano, con una mezcla de reacción PCR, comprendiendo dicha mezcla de reacción un par de cebadores que permiten amplificar una secuencia diana altamente conservada en el genoma bacteriano, bajo condiciones en las que dicha secuencia diana es amplificada para formar un producto de amplificación; y (iii) analizar el producto de amplificación para determinar la presencia o ausencia de ADN bacteriano que lleva la secuencia diana seleccionada, en donde la detección de ADN bacteriano en dicha muestra es indicativa de la presencia de bacterias en dicho sujeto. De aplicación en la determinación de la existencia de bacterias circulantes en un sujeto, especialmente en un paciente asintomático.
ES200200677A 2002-03-22 2002-03-22 Metodo para la deteccion de presencia bacteriana en un sujeto. Expired - Fee Related ES2193873B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES200200677A ES2193873B1 (es) 2002-03-22 2002-03-22 Metodo para la deteccion de presencia bacteriana en un sujeto.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES200200677A ES2193873B1 (es) 2002-03-22 2002-03-22 Metodo para la deteccion de presencia bacteriana en un sujeto.

Publications (2)

Publication Number Publication Date
ES2193873A1 ES2193873A1 (es) 2003-11-01
ES2193873B1 true ES2193873B1 (es) 2004-08-16

Family

ID=29763006

Family Applications (1)

Application Number Title Priority Date Filing Date
ES200200677A Expired - Fee Related ES2193873B1 (es) 2002-03-22 2002-03-22 Metodo para la deteccion de presencia bacteriana en un sujeto.

Country Status (1)

Country Link
ES (1) ES2193873B1 (es)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991014002A2 (en) * 1990-03-07 1991-09-19 F.Hoffmann-La Roche Ag Method for diagnosis of lyme disease
ES2112824T3 (es) * 1986-11-24 1998-04-16 Gen Probe Inc Sondas de acidos nucleicos para deteccion y/o cuantificacion de organismos no virales.
WO2002010444A1 (en) * 2000-07-28 2002-02-07 University Of Sydney A method of detecting microorganisms

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2112824T3 (es) * 1986-11-24 1998-04-16 Gen Probe Inc Sondas de acidos nucleicos para deteccion y/o cuantificacion de organismos no virales.
WO1991014002A2 (en) * 1990-03-07 1991-09-19 F.Hoffmann-La Roche Ag Method for diagnosis of lyme disease
WO2002010444A1 (en) * 2000-07-28 2002-02-07 University Of Sydney A method of detecting microorganisms

Also Published As

Publication number Publication date
ES2193873A1 (es) 2003-11-01

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