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ES2191028T3 - Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado. - Google Patents

Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado.

Info

Publication number
ES2191028T3
ES2191028T3 ES94904344T ES94904344T ES2191028T3 ES 2191028 T3 ES2191028 T3 ES 2191028T3 ES 94904344 T ES94904344 T ES 94904344T ES 94904344 T ES94904344 T ES 94904344T ES 2191028 T3 ES2191028 T3 ES 2191028T3
Authority
ES
Spain
Prior art keywords
plants
plant
modified
obtaining
fructosiltranspherase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94904344T
Other languages
English (en)
Inventor
Josephus Christianus Smeekens
Michael Johannes Marcu Ebskamp
Petrus Jacobus Weisbeek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
STICHTING SCHEIKUNDIG ONDERZOE
Original Assignee
STICHTING SCHEIKUNDIG ONDERZOE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from NL9300646A external-priority patent/NL9300646A/nl
Application filed by STICHTING SCHEIKUNDIG ONDERZOE filed Critical STICHTING SCHEIKUNDIG ONDERZOE
Application granted granted Critical
Publication of ES2191028T3 publication Critical patent/ES2191028T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/20—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents
    • A23L29/206—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin
    • A23L29/244—Foods or foodstuffs containing additives; Preparation or treatment thereof containing gelling or thickening agents of vegetable origin from corms, tubers or roots, e.g. glucomannan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Physiology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cultivation Of Plants (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Packaging Of Special Articles (AREA)
  • Paper (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

EL INVENTO TRATA DE UN METODO PARA OBTENER PLANTAS TRANSGENICAS QUE MUESTRAN UN MODELO DE FRUCTAN MODIFICADO COMPARADAS CON PLANTAS NO TRANFORMADAS, COMPRENDIENDO LOS SIGUIENTES PASOS: A) PREPARAR UNA CONSTRUCCION DE DNA QUE COMPRENDA UN O MAS GENES FRUCTOSILTRANSFERASA, O VERSIONES MODIFICADAS DE LOS MISMOS, SIENDO LIGABLES OPERACIONALMENTE A UNA SECUENCIA PROMOTORA ACTIVA EN PLANTAS Y UNA SECUENCIA TERMINADORA ACTIVA EN PLANTAS; B) TRANSFORMAR UNA CELULA DE PLANTA CON LA CONSTRUCCION; Y C) REGENERAR UNA PLANTA TRANSGENICA DE LA CELULA TRANSFORMADA DE LA PLANTA. PREFERIBLEMENTE, LA REGION 5`INAMOVIBLE DEL GEN FRUCTOSILTRANSFERASA SE MODIFICA DE TAL MANERA QUE LA EXPRESION DE LA FRUCTOSILTRANSFERASA NO SE VEA AFECTADA NEGATIVAMENTE. LA CONSTRUCCION DE DNA, PREFERIBLEMENTE, TAMBIEN COMPRENDE LA SECUENCIA OBJETIVA DE MOVIMIENTO DEL GEN DE LA FRUCTOSILTRANSFERASA PARA DIRIGIRLA AL TEJIDO ESPECIFICO DE LA PLANTA O AL COMPARTIMENTO CELULAR DE LA MISMA. LA FRUCTOSILTRANSFERASA PRODUCIDA EN LAS PLANTAS TRANSGENICAS CAMBIANDO EL MODELO FRUCTAN DE LAS PLANTAS PRINCIPALES AL COMPORTAMIENTO DE DIFERENTES PLANTAS DEBIDO A ALTERACIONES EN SU ORIGEN Y EN LA PRODUCCION, O INCREMENTA LA TOLERANCIA A LA SEQUIA, FRIO, U OTROS FACTORES DE ESTRES, CONTIENE UN INDICE MAYOR DE SEQUEDAD, MEJOR SABOR Y ALMACENAMIENTO Y SE MEJORA SU VALOR NUTRITIVO. LAS PLANTAS PUEDEN SER TAMBIEN USADAS COMO MATERIA CRUDA PARA LA PRODUCCION DE FRUCTAN.
ES94904344T 1992-12-28 1993-12-28 Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado. Expired - Lifetime ES2191028T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP92204098 1992-12-28
NL9300646A NL9300646A (nl) 1992-12-28 1993-04-15 Werkwijze voor het verkrijgen van transgene planten welke een gemodificeerd fructaan-patroon vertonen.

Publications (1)

Publication Number Publication Date
ES2191028T3 true ES2191028T3 (es) 2003-09-01

Family

ID=26131899

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94904344T Expired - Lifetime ES2191028T3 (es) 1992-12-28 1993-12-28 Metodo para obtener plantas transgenicas que presentan un patron de fructano modificado.

Country Status (12)

Country Link
US (2) US6025542A (es)
EP (1) EP0677112B1 (es)
JP (1) JPH08507918A (es)
AT (1) ATE235557T1 (es)
AU (1) AU5843794A (es)
DE (1) DE69332803T2 (es)
DK (1) DK0677112T3 (es)
ES (1) ES2191028T3 (es)
HU (1) HU225704B1 (es)
PL (1) PL178789B1 (es)
PT (1) PT677112E (es)
WO (1) WO1994014970A1 (es)

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US6329574B1 (en) 1990-01-22 2001-12-11 Dekalb Genetics Corporation High lysine fertile transgenic corn plants
HU220773B1 (hu) 1990-01-22 2002-05-28 Dekalb Genetics Corporation Eljárás termő transzgenikus kukoricanövények előállítására
DE4227061A1 (de) * 1992-08-12 1994-02-17 Inst Genbiologische Forschung DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
AU5843794A (en) * 1992-12-28 1994-07-19 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
US6326527B1 (en) 1993-08-25 2001-12-04 Dekalb Genetics Corporation Method for altering the nutritional content of plant seed
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US5780709A (en) * 1993-08-25 1998-07-14 Dekalb Genetics Corporation Transgenic maize with increased mannitol content
EP0728213B2 (en) * 1993-11-09 2008-12-10 E.I. Du Pont De Nemours And Company Transgenic fructan accumulating crops and methods for their production
NL1000064C1 (nl) 1994-07-08 1996-01-08 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
NL9401140A (nl) * 1994-07-08 1996-02-01 Stichting Scheikundig Onderzoe Produktie van oligosacchariden in transgene planten.
AU4634396A (en) * 1995-01-06 1996-07-24 Centrum Voor Plantenveredelings- En Reproduktieonderzoek (Cpro - Dlo) Dna sequences encoding carbohydrate polymer synthesizing enzymes and method for producing transgenic plants
ATE393231T1 (de) 1995-12-28 2008-05-15 Suntory Ltd Verfahren zur herstellung temperatur-toleranter pflanzen
US6833491B2 (en) 1996-02-07 2004-12-21 D. J. Van Der Have B.V. Modification of polysaccharides
NL1002275C2 (nl) * 1996-02-07 1997-08-08 Have D J Van Der Bv Modificatie van polysacchariden.
DE19617687C2 (de) * 1996-05-03 2000-11-16 Suedzucker Ag Verfahren zur Herstellung transgener, Inulin erzeugender Pflanzen
BE1010449A3 (fr) 1996-08-01 1998-08-04 Raffinerie Tirlemontoise Sa Procede de preparation d'une composition polydipersee de saccharides, composition polydispersee de saccharides, produit alimentaire, pharmaceutique et/ou cosmetique comprenant ladite composition polydispersee.
DE19708774A1 (de) * 1997-03-04 1998-09-17 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme die Fructosylpolymeraseaktivität besitzen
DE19749122A1 (de) 1997-11-06 1999-06-10 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen
US6365800B1 (en) * 1998-03-12 2002-04-02 E. I. Du Pont Nemours & Company Transgenic crops accumulating fructose polymers and methods for their production
CN1116417C (zh) * 1998-04-08 2003-07-30 中国科学院遗传研究所 用转基因技术提高植物耐盐性的方法
DE19840028A1 (de) * 1998-09-02 2000-03-09 Max Planck Gesellschaft Nucleinsäuremoleküle codierend Enzyme, die Fructosyltransferaseaktivität besitzen, und deren Verwendung
JP2000350583A (ja) * 1999-03-25 2000-12-19 Hokkaido National Agricultural Experiment Station 低温発現型フルクタン代謝酵素遺伝子
CA2331674A1 (en) * 2000-01-28 2001-07-28 Southern Illinois University Isolated polynucleotides and polypeptides relating to loci underlying resistance to soybean cyst nematode and soybean sudden death syndrome and methods employing same
US20040064846A1 (en) * 2001-02-28 2004-04-01 Henry Daniell Genetic engineering of drought tolerance via a plastid genome
AUPQ815500A0 (en) 2000-06-14 2000-07-06 Molecular Plant Breeding Nominees Ltd Modification of fructan biosynthesis
US6791015B2 (en) 2000-10-30 2004-09-14 E. I. Du Pont De Nemours And Company Fructan biosynthetic enzymes
US20040086902A1 (en) * 2000-12-21 2004-05-06 Dirk Engels Method for the production of polyfructans
US7608754B2 (en) * 2001-06-25 2009-10-27 Ses Europe N.V./S.A. Double fructan beets
US20050150008A1 (en) * 2001-11-07 2005-07-07 Agrigenesis Biosciences Limited Compositions isolated from forage grasses and methods of use
AU2002360228B8 (en) * 2001-11-07 2008-12-04 Genesis Research And Development Corporation Limited Compositions from the grasses lolium perenne and festuca arundinacea
US20040133944A1 (en) * 2003-01-08 2004-07-08 Delta And Pine Land Company Seed oil suppression to enhance yield of commercially important macromolecules
AU2003902253A0 (en) * 2003-05-12 2003-05-29 The University Of Queensland Method for increasing product yield
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JP5805534B2 (ja) * 2008-09-15 2015-11-04 アグリカルチャー ビクトリア サービシーズ プロプライエタリー リミテッド フルクタン生合成の修飾、植物バイオマスの増大、および植物における生化学的経路の生産性を増強する方法
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DE4227061A1 (de) * 1992-08-12 1994-02-17 Inst Genbiologische Forschung DNA-Sequenzen, die in der Pflanze die Bildung von Polyfructanen (Lävanen) hervorrufen, Plasmide enthaltend diese Sequenzen sowie Verfahren zur Herstellung transgener Pflanzen
AU5843794A (en) * 1992-12-28 1994-07-19 Stichting Scheikundig Onderzoek In Nederland Method for obtaining transgenic plants showing a modified fructan pattern
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EP0728213B2 (en) * 1993-11-09 2008-12-10 E.I. Du Pont De Nemours And Company Transgenic fructan accumulating crops and methods for their production

Also Published As

Publication number Publication date
PT677112E (pt) 2003-08-29
PL178789B1 (pl) 2000-06-30
JPH08507918A (ja) 1996-08-27
EP0677112B1 (en) 2003-03-26
EP0677112A1 (en) 1995-10-18
HU9501912D0 (en) 1995-08-28
DK0677112T3 (da) 2003-07-21
US6025542A (en) 2000-02-15
ATE235557T1 (de) 2003-04-15
AU5843794A (en) 1994-07-19
HUT71782A (en) 1996-02-28
US5986173A (en) 1999-11-16
WO1994014970A1 (en) 1994-07-07
DE69332803T2 (de) 2004-03-04
HU225704B1 (en) 2007-06-28
PL309606A1 (en) 1995-10-30
DE69332803D1 (de) 2003-04-30

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